Article
Chemistry, Analytical
Beatriz Lozano-Torres, Juan F. Blandez, Irene Galiana, Jose A. Lopez-Dominguez, Miguel Rovira, Marta Paez-Ribes, Estela Gonzalez-Gualda, Daniel Munoz-Espin, Manuel Serrano, Felix Sancenon, Ramon Martinez-Manez
Summary: Advances in the detection of cellular senescence are crucial for monitoring age-related diseases, and the use of the HeckGal probe allows reliable detection of senescence both in vitro and in vivo. This technology provides a potential tool for detecting senescence in aged or damaged tissues.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Nicolas Malausse, Sylvie van der Werf, Nadia Naffakh, Sandie Munier
Summary: Co-infections between influenza A virus (IAV) and influenza B virus (IBV) were studied by infecting A549 cells with recombinant IAV-GFP and IBV-mCherry. Surprisingly, IBV replication was enhanced when co-infected with IAV, particularly when IAV was added 1 hour before IBV. This enhancing effect was dependent on transcription/replication of the IAV genome.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yong-Xiang Wu, Dailiang Zhang, Xiaoxiao Hu, Ruizi Peng, Junbin Li, Xiaobing Zhang, Weihong Tan
Summary: Novel theranostic nanosystems based on two-photon fluorescence have been designed and prepared for the diagnosis and therapy of various cancers. These nanosystems can be used for intracellular imaging and drug delivery in cancer cells, enhancing imaging depth and tissue spatial localization.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Immunology
Alvaro Lopez-Valinas, Marta Valle, Miaomiao Wang, Ayub Darji, Guillermo Cantero, Chiara Chiapponi, Joaquim Segales, Llilianne Ganges, Jose I. Nunez
Summary: Influenza A viruses have the ability to infect a wide range of bird and mammal species. They constantly evolve due to low proofreading activity and genomic reassortment, posing a threat to human and animal health. This study found that vaccination can reduce the likelihood of genomic reassortment and may influence the evolution of swine influenza viruses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Developmental Biology
Sydney Leaman, Nicolas Marichal, Benedikt Berninger
Summary: Cellular identity can be reprogrammed through gene regulatory networks, and recent research suggests that this reprogramming can mitigate disease phenotypes.
Article
Multidisciplinary Sciences
Joseph D. Trimarco, Sarah L. Nelson, Ryan R. Chaparian, Alexandra Wells, Nathan B. Murray, Parastoo Azadi, Carolyn B. Coyne, Nicholas S. Heaton
Summary: The study identifies the glycosyltransferase B3GAT1 as a host protein that restricts a broad spectrum of influenza and other respiratory viruses in vitro and in mice when ectopically overexpressed.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Xing Xie, Maoda Pang, Shan Liang, Yan Lin, Yanbing Zhao, Dong Qiu, Jin Liu, Yuhao Dong, Yongjie Liu
Summary: The study found that miRNAs associated with innate immunity and inflammatory response, cfa-miR-125b and cfamiR-151, were significantly decreased in CIV-infected canine primary cells, suggesting they may act as negative regulators of CIV replication.
VETERINARY MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xinyu Miao, Yinyan Yin, Yulian Chen, Wenhui Bi, Yuncong Yin, Sujuan Chen, Daxin Peng, Lizeng Gao, Tao Qin, Xiufan Liu
Summary: A novel multi-antiviral metastable iron sulfides (mFeS) has been developed to target various influenza A/B subtype viruses. mFeS induces high levels of lipid peroxidation and center dot OH free radicals in the viral envelope, leading to viral ferroptosis and the loss of viral infectibility and pathogenicity. The decoction of mFeS inhibits intracellular viral replication by correcting virus-induced reprogrammed sulfur metabolism. mFeS represents a broad-spectrum antiviral alternative with potential therapeutic effect against influenza virus.
Article
Materials Science, Multidisciplinary
Tiziana Ritacco, Dante M. Aceti, Gianfranco De Domenico, Michele Giocondo, Alfredo Mazzulla, Gabriella Cipparrone, Pasquale Pagliusi
Summary: Cholesteric liquid crystals with self-organized helical arrangement allow for tuning the structure to achieve custom optical properties, demonstrated by polymeric microstructures with tailored photonic band gaps.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Immunology
Jenna M. Kastenschmidt, Suhas Sureshchandra, Aarti Jain, Jenny E. Hernandez-Davies, Rafael de Assis, Zachary W. Wagoner, Andrew M. Sorn, Mahina Tabassum Mitul, Aviv I. Benchorin, Elizabeth Levendosky, Gurpreet Ahuja, Qiu Zhong, Douglas Trask, Jacob Boeckmann, Rie Nakajima, Algimantas Jasinskas, Naresha Saligrama, D. Huw Davies, Lisa E. Wagar
Summary: This study investigated the effects of different antigen formats on mucosal adaptive immune responses using a human tonsil organoid model. The results showed significant differences in the B and T cell responses elicited by different antigen formats, which led to changes in the corresponding antibody response. The ability of antigen formats to recruit naive and memory B and T cells to the response was identified as a major source of variability.
Article
Biochemistry & Molecular Biology
Takashi Saitou, Takeshi Imamura
Summary: This study aimed to construct a light-sheet microscope with higher resolution imaging while maintaining a large field of view. They used a low magnification (16x) and high numerical aperture (0.8) objective to address potential illumination and detection mismatch. By applying a depth of focus extension method, they demonstrated that the extended DOF system combined with wide-field two-photon light-sheet microscopy offers a simple and easy setup for live imaging of large multicellular organisms with sub-cellular resolution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Spectroscopy
Yu-Xu Tu, Natarajan Vijay, Han-Xiang Ko, Yuan-Pin Lo, Sivan Velmathi, Shu-Pao Wu
Summary: The new fluorescent probe ANP can rapidly and efficiently detect cysteine, and it can evaluate the distribution of cysteine in living cells and tissues.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Barbara Szutkowska, Klaudia Wieczorek, Ryszard Kierzek, Pawel Zmora, Jake M. Peterson, Walter N. Moss, David H. Mathews, Elzbieta Kierzek
Summary: This study proposes a secondary structure for segment 8 vRNA of A/California/04/2009 (H1N1) for the first time and highlights the location of conserved structural motifs within IAV strains. The research has implications for understanding the functionality of viral RNA structures and developing inhibition methods against influenza A virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Katherine Kaugars, Joseph Dardick, Anna Paula de Oliveira, Kayla A. Weiss, Regy Lukose, John Kim, Lawrence Leung, Saranathan Rajagopalan, Sydney Wolin, Leor Akabas, David M. Knipe, Goran Bajic, William R. Jacobs Jr
Summary: Despite annual vaccination efforts, influenza continues to cause significant morbidity and mortality worldwide. To improve vaccine efficacy, researchers are exploring the use of antibodies that can activate immune cell effector functions, such as ADCC. Evidence suggests that ADCC is crucial for protection against influenza and other infectious diseases, leading to the development of innovative vaccine strategies such as using Delta gD-2 as a vector for a broadly protective influenza vaccine.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Vanessa Gerlt, Juliane Mayr, Juliana Del Sarto, Stephan Ludwig, Yvonne Boergeling
Summary: The study demonstrates the importance of PP2A in efficient replication of various IAV subtypes, as decreased PP2Ac levels result in reduced cell viability and increased cell death after IAV infection. This is attributed to a synergistic action of hyper-activated PI3K/Akt, MAPK/JAK-STAT, and NF-kB signaling pathways related to apoptosis, indicating the critical role of PP2A in orchestrating cell survival mechanisms during IAV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Prarthana J. Dalal, David P. Sullivan, Evan W. Weber, David B. Sacks, Matthias Gunzer, Isabella M. Grumbach, Joan Heller Brown, William A. Muller
Summary: The research highlights the important roles of endothelial CaM and CaMKIIδ in transducing specific calcium signals required for TEM, which interferes with the delivery of the lateral border recycling compartment (LBRC) and reduces TEM.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Immunology
Jack Winneberger, Sebastian Schoels, Katrin Lessmann, Javier Randez-Garbayo, Alexander T. Bauer, Ayan Mohamud Yusuf, Dirk M. Hermann, Matthias Gunzer, Stefan W. Schneider, Jens Fiehler, Christian Gerloff, Mathias Gelderblom, Peter Ludewig, Tim Magnus
Summary: The study demonstrated the role of PECAM-1 in regulating trans-endothelial immune cell migration in ischemic brain injury using genetic Pecam-1 depletion and PECAM-1 blockade. The inhibition of PECAM-1 showed reduced infarct volume, edema formation, and brain atrophy in mice. Blocking PECAM-1 could potentially be a target to reduce post-stroke neuroinflammation and enhance post-ischemic brain remodeling.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Immunology
Kerstin Muelling, Alexa Josephine Fischer, Elena Siakaeva, Mathis Richter, Sharareh Bordbari, Ilona Spyra, Christian Koester, Dirk M. Hermann, Matthias Gunzer, Ursula Felderhoff-Mueser, Ivo Bendix, Jadwiga Jablonska, Josephine Herz
Summary: This study found that neutrophils in the neonatal brain are rapidly activated after experimental hypoxia-ischemia, producing reactive oxygen species and showing an increased proportion of hyperactivated/aged cells compared to peripheral neutrophils. Delayed neutrophil depletion led to reduced cellular neurodegeneration and decreased microglial and astroglial activation, offering new possibilities for therapeutic approaches in post-hypoxic brain injury.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Immunology
Leoni Rolfes, Monika Riek-Burchardt, Marc Pawlitzki, Jens Minnerup, Stefanie Bock, Mariella Schmidt, Sven G. Meuth, Matthias Gunzer, Jens Neumann
Summary: This study characterized the dynamics of neutrophil granulocytes in two models of permanent stroke, confirming their involvement in capillary stalls in permanent cerebral ischemia. Flow cytometric analysis revealed an increase in neutrophil proportion with selective upregulation of endothelial adherence markers in peripheral blood. The dynamic microcirculatory stall phenomenon after transient ischemia followed by reperfusion also occurs after permanent small- or large-vessel stroke and is clearly attributable to neutrophils.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Cell Biology
Michaela Duemig, Jasmin Binder, Anastasia Gaculenko, Franziska Daul, Lex Winandy, Mike Hasenberg, Matthias Gunzer, Reinhard Fischer, Markus Kuenzler, Sven Krappmann
Summary: Fungal spores play a crucial role in dispersal and survival, with specialized spores serving as infectious particles. The pathogenic fungus Aspergillus fumigatus produces conidia, which contain antimicrobial peptides that provide a growth advantage over bacterial competitors. This has implications for the ecological niche of A. fumigatus and its infectious process in causing aspergillosis.
CELLULAR MICROBIOLOGY
(2021)
Article
Neurosciences
Ayan Mohamud Yusuf, Nina Hagemann, Sarah Schulten, Olessja Rausch, Kristina Wagner, Tanja Hussner, Yachao Qi, Matthias Totzeck, Christoph Kleinschnitz, Anthony Squire, Matthias Gunzer, Dirk M. Hermann
Summary: The advancement of light sheet microscopy enables 3D analysis of cerebral microvasculature in cleared brains. The technique of reverse clearing and rehydration allows for immunohistochemistry in cleared brains. Microvascular remodeling in the early phase after stroke shows reduced branching point density.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Dirk M. Hermann, Matthias Gunzer
Summary: Microglia play a vital role in brain remodeling and repair after injury, potentially offering opportunities for promoting neurological recovery in human patients. Through the development of pharmacological and transgenic mouse models, researchers can gain valuable insights into the functions of microglia in neurodegenerative and neurorestorative processes.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Cardiac & Cardiovascular Systems
Tienush Rassaf, Matthias Totzeck, Amir A. Mahabadi, Ulrike Hendgen-Cotta, Sebastian Korste, Stephan Settelmeier, Peter Luedike, Ulf Dittmer, Frank Herbstreit, Thorsten Brenner, Karin Klingel, Mike Hasenberg, Bernd Walkenfort, Matthias Gunzer, Thomas Schlosser, Alexander Weymann, Markus Kamler, Bastian Schmack, Arjang Ruhparwar
Summary: COVID-19 poses challenges in caring for cardiovascular patients, but LVAD implantation may be a successful treatment for pre-diseased hearts affected by the virus.
Article
Immunology
Vikramjeet Singh, Alexander Beer, Andreas Kraus, Felix Mang, Xiaoni Zhang, Jinhua Xue, Nina Hagemann, Dirk M. Hermann, Matthias Gunzer
Summary: The study found specific increases in alveolar ACE2 levels and inflammation in murine lungs after experimental stroke, suggesting that patients with brain injuries may have increased binding affinity to SARS-CoV-2 in their lungs, potentially explaining the increased susceptibility to develop COVID-19 in stroke patients.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Cardiac & Cardiovascular Systems
Jonas Gregorius, Chen Wang, Oumaima Stambouli, Tanja Hussner, Yachao Qi, Tobias Tertel, Verena Borger, Ayan Mohamud Yusuf, Nina Hagemann, Dongpei Yin, Robin Dittrich, Yanis Mouloud, Fabian D. Mairinger, Fouzi El Magraoui, Aurel Popa-Wagner, Christoph Kleinschnitz, Thorsten R. Doeppner, Matthias Gunzer, Helmut E. Meyer, Bernd Giebel, Dirk M. Hermann
Summary: Obtained from the right cell-type, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) promote stroke recovery by enhancing microvascular remodeling. In vitro, sEVs from hypoxic MSCs, but not 'normoxic' MSCs, dose-dependently promoted endothelial proliferation, migration, and tube formation, as well as increased post-ischemic endothelial survival.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Article
Multidisciplinary Sciences
Korbinian Kienle, Katharina M. Glaser, Sarah Eickhoff, Michael Mihlan, Konrad Knoepper, Eduardo Reategui, Maximilian W. Epple, Matthias Gunzer, Ralf Baumeister, Teresa K. Tarrant, Ronald N. Germain, Daniel Irimia, Wolfgang Kastenmueller, Tim Laemmermann
Summary: The study found that neutrophils have evolved an intrinsic mechanism to limit self-aggregation during inflammation, with GPCR desensitization acting as a negative feedback control to stop migration when high concentrations of attractants are detected. However, interfering with this process may hinder bacterial clearance, as bacteria proliferation within neutrophil clusters becomes impeded. This highlights the delicate balance of neutrophil chemotaxis and arrest in combating bacterial escape.
Article
Immunology
I-Jung Lee, Yu-Hua Lan, Ping-Yi Wu, Yan-Wei Wu, Yu-Hung Chen, Sheng-Che Tseng, Tzu-Jiun Kuo, Cheng-Pu Sun, Jia-Tsrong Jan, Hsiu-Hua Ma, Chun-Che Liao, Jian-Jong Liang, Hui-Ying Ko, Chih-Shin Chang, Wen-Chun Liu, Yi-An Ko, Yen-Hui Chen, Zong-Lin Sie, Szu- Tsung, Yi-Ling Lin, I-Hsuan Wang, Mi-Hua Tao
Summary: This study developed a subunit vaccine, ASD254, encapsulating the SARS-CoV-2 spike receptor-binding domain (RBD) protein using a nanoparticle vaccine platform. ASD254 showed higher RBD-specific antibody titers and generated significantly more neutralizing antibodies compared to the aluminum-adjuvant RBD vaccine. Mice vaccinated with ASD254 exhibited protective immune responses against SARS-CoV-2, with no detectable viral loads and reduced lung lesions, and the neutralizing antibodies lasted for at least one year and were effective against various SARS-CoV-2 variants.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Biotechnology & Applied Microbiology
Cheng-Pu Sun, Chi-Wen Chiu, Ping-Yi Wu, Szu- Tsung, I-Jung Lee, Chih-Wei Hu, Min-Feng Hsu, Tzu-Jiun Kuo, Yu-Hua Lan, Li-Yao Chen, Hui-Yee Ng, Meng-Jhe Chung, Hsin-Ni Liao, Sheng-Che Tseng, Chia-Hui Lo, Yung-Jiun Chen, Chun-Che Liao, Chih-Shin Chang, Jian-Jong Liang, Piotr Draczkowski, Sarita Puri, Yuan-Chih Chang, Jing-Siou Huang, Cheng-Cheung Chen, Jyh-Hwa Kau, Yen-Hui Chen, Wen-Chun Liu, Han-Chun Wu, Shang-Te Danny, I-Hsuan Wang, Mi-Hua Tao
Summary: The study isolated and humanized a monoclonal anti-human angiotensin-converting enzyme 2 (hACE2) antibody, ch2H2, which effectively blocked the binding of viral receptor-binding domain (RBD) to hACE2. In vitro and in vivo experiments demonstrated that the ch2H2 antibody exhibited potent inhibitory activity against multiple SARS-CoV-2 variants, including the Omicron variant. The adeno-associated virus (AAV)-mediated delivery of the antibody also showed promising results in reducing viral load and mitigating pulmonary pathological changes.
Article
Cell Biology
Chao-Fu Yang, Chun-Che Liao, Hung-Wei Hsu, Jian-Jong Liang, Chih-Shin Chang, Hui-Ying Ko, Rue-Hsin Chang, Wei-Chun Tang, Ming-Hao Chang, I-Hsuan Wang, Yi-Ling Lin
Summary: The expression level of hACE2 controls the mode of SARS-CoV-2 transmission. Cells with limited hACE2 expression have an advantage for cell-free transmission, while enhanced hACE2 expression facilitates cell-to-cell transmission. This cell-to-cell transmission is likely facilitated by hACE2-containing vesicles.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Cell Biology
I-Jung Lee, Cheng-Pu Sun, Ping-Yi Wu, Yu-Hua Lan, I-Hsuan Wang, Wen-Chun Liu, Joyce Pei-Yi Yuan, Yu-Wei Chang, Sheng-Che Tseng, Szu- Tsung, Yu-Chi Chou, Monika Kumari, Yin-Shiou Lin, Hui-Feng Chen, Tsung-Yen Chen, Chih-Chao Lin, Chi-Wen Chiu, Chung-Hsuan Hsieh, Cheng-Ying Chuang, Chao-Min Cheng, Hsiu-Ting Lin, Wan-Yu Chen, Fu-Fei Hsu, Ming-Hsiang Hong, Chun-Che Liao, Chih-Shin Chang, Jian-Jong Liang, Hsiu-Hua Ma, Ming-Tsai Chiang, Hsin-Ni Liao, Hui-Ying Ko, Liang-Yu Chen, Yi-An Ko, Pei-Yu Yu, Tzu-Jing Yang, Po-Cheng Chiang, Shang-Te Hsu, Yi-Ling Lin, Chong-Chou Lee, Han-Chung Wu, Mi-Hua Tao
Summary: This study reports an mRNA-based vaccine using an engineered hybrid receptor binding domain that can generate neutralizing antibodies and T cell responses against various SARS-CoV-2 variants. The results demonstrate that including different antigenic mutations from different variants is a feasible approach to develop vaccines with broad protection.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)