Article
Agronomy
Cao Zhi, Muhammad Moaaz Ali, Shariq Mahmood Alam, Shaista Gull, Sajid Ali, Ahmed F. Yousef, Mohamed A. A. Ahmed, Songfeng Ma, Faxing Chen
Summary: This study identified and characterized the PEPC gene family in Rosaceae species, including loquat, apple, peach, strawberry, and pear. The results showed that PEPC genes have multiple potential roles in plant metabolism and have undergone duplication and selection during evolution.
Article
Plant Sciences
Marco Cirilli, Stefano Gattolin, Remo Chiozzotto, Irina Baccichet, Thierry Pascal, B. N. Dicte Quilot-Turion, Laura Rossini, Daniele Bassi
Summary: This study identified a major QTL capable of delaying blooming date in peach by increasing heat requirement. A genetic variant (pet DEL) in the PETALOSA gene was found to be strictly associated with this QTL, affecting both flower morphology and flowering time. Analysis on segregating genome-edited tobacco plants further confirmed the potential ability of PET variations to delay flowering time.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Food Science & Technology
Qiaoyu Sun, Wenqing Xu, Yanan Liu, Shengnan Zhan, Xingfeng Shao, Zufang Wu, Peifang Weng, Kejun Cheng, Xin Zhang
Summary: The study shows that peach polysaccharides (PPS) can regulate the structural disorder of intestinal microbiota, ameliorate cell dysfunction, and have preventive and therapeutic effects on circadian rhythm disorder and related cognitive impairment.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Plant Sciences
Ana Puertes, Helin Polat, Luis Andres Ramon-Nunez, Matilde Gonzalez, Gema Ancillo, Elena Zuriaga, Gabino Rios
Summary: Transcriptomic and gene expression analysis help identify and characterize transcriptional regulatory factors and effectors in bud development. Single-bud gene analysis in peach flower buds reveals the variability in gene expression during dormancy release, contributing to the understanding of co-expression modules and regulatory aspects in this tissue.
Article
Genetics & Heredity
Silvia Farinati, Cristian Forestan, Monica Canton, Giulio Galla, Claudio Bonghi, Serena Varotto
Summary: Research shows that a slow ripening phenotype in peach is associated with misregulation of genes involved in mesocarp identity, resulting in reduced fruit size. Endoreduplication progressively increases in mesocarp during fruit development, occurring in normal fruit but not in slow ripening fruit.
Article
Horticulture
Haiying Yue, Yaming Yang, Guili Cha, Shichang Ren, Jiyan Wang, Xiaoyan Shi, Qinggang Zhu, Qianjin Li, Xiaolin Ren, Jianwen Tian, Yuduan Ding
Summary: This study investigated the function of the BES1 gene family in 48 horticultural crops through phylogenetic and genomic colinearity network analysis. Transcriptional regulatory networks during various conditions in kiwifruit and peach were also analyzed. The results provide a comprehensive understanding of the phylogenetic relationships of the BES1 family in plant genomes and predictions of growth and development of kiwifruit and peach fruits.
Article
Plant Sciences
Shuaishuai Wang, Mengyun Shi, Yang Zhang, Zhifei Pan, Xingbin Xie, Linzhong Zhang, Peipei Sun, Huan Feng, Hao Xue, Congbing Fang, Jing Zhao
Summary: FaMYB63 is a transcription factor that plays a crucial role in regulating eugenol biosynthesis in strawberry fruit. The study found that FaMYB63 directly activates the biosynthetic genes involved in the phenylpropanoid pathway, leading to the accumulation of eugenol. These findings are important for understanding the regulatory network of eugenol metabolism in edible fruits.
Article
Plant Sciences
Xiaohan Jiang, Kangchen Liu, Huixiang Peng, Jing Fang, Aidi Zhang, Yuepeng Han, Xiujun Zhang
Summary: In this study, a 3D transcriptome data of five different peach varieties was used to construct gene co-expression networks during fruit ripening. Comparative analysis of these networks identified modules related to organic acid synthesis and metabolism under high-acid and low-acid conditions, and 20 candidate genes were identified as regulatory factors for organic acid metabolism in peach.
Article
Biochemistry & Molecular Biology
Yuntian Ye, Yongqiang Liu, Xiaolong Li, Guangyi Wang, Quan Zhou, Qing Chen, Jiale Li, Xiaorong Wang, Haoru Tang
Summary: This study identified BBX transcription factors in cultivated and wild strawberries, revealing the expansion of gene family is related to whole-genome or segmental duplication. Loss and acquisition events of FaBBX genes might affect agronomic traits, and some FaBBX genes showed changes in transcriptional activity in response to blue light treatment. FaBBX28c1, in particular, could play a crucial role in flowering time regulation in cultivated strawberry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Zhiqi Li, Qian Xie, Jiahui Yan, Jianqing Chen, Qingxi Chen
Summary: This study identified 10 GRF genes in the genome of the diploid woodland strawberry and found that these genes potentially play roles in the growth, development, and stress response of plants. The results provide a theoretical and empirical foundation for understanding the mechanisms of abiotic stress responses in strawberry.
Article
Plant Sciences
Yibo Bai, Hui Liu, Haimeng Lyu, Liyao Su, Jinsong Xiong, Zong-Ming (Max) Cheng
Summary: By using single-cell RNA-seq technology, this study analyzed the gene expression patterns in leaves of woodland strawberry during infection by Botrytis cinerea, and identified the signaling and gene expression patterns among different cell types. The results revealed the defense response of plants during infection and provide guidance for the identification of disease resistance genes and the development of resistant cultivars.
HORTICULTURE RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Zhipeng Sun, Todd G. Nystul, Guohua Zhong
Summary: In this study, the researchers used single-cell RNA sequencing, RNAi screen, and bioinformatic analysis to identify genes involved in germ cell differentiation. They discovered that a gene named eggpl is highly expressed in ovarian germline stem cells and downregulated in early daughter cells. Knockdown of eggpl resulted in defects in germ cell proliferation and differentiation. Furthermore, the downregulation of eggpl was found to link nutritional status to germ cell proliferation and differentiation. This study provides new insights into the signaling networks regulating early germ cell development and identifies eggpl as an important player in this process.
Article
Biochemistry & Molecular Biology
Qiuju Chen, Min Lian, Jian Guo, Binbin Zhang, Sankui Yang, Kexin Huang, Futian Peng, Yuansong Xiao
Summary: Nitrogen is an important nutrient element, but excessive nitrogen has negative effects on plants and the environment. This study revealed the different responses of two peach rootstocks to KNO3, identifying differentially expressed genes (DEGs) involved in nitrogen metabolism and plant hormone signal transduction pathways. The functional role of PpNRT2.1 in nitrogen metabolism was verified through heterologous expression in Arabidopsis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Mengying Zhang, Xiyun Jin, Junyi Li, Yi Tian, Qi Wang, Xinhui Li, Juan Xu, Yongsheng Li, Xia Li
Summary: CeRNASeek is an R package that integrates multiple-omics data to identify and analyze ceRNA-ceRNA interactions. It provides six computational methods and various downstream analyses, suitable for studying the function of ceRNAs in complex diseases.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Federico Bocci, Peijie Zhou, Qing Nie
Summary: Using spliceJAC, a method to quantify multivariate mRNA splicing from single-cell RNA sequencing, enables the extraction of dynamical information from single-cell transcriptomics. By applying spliceJAC to biological systems, specific signaling genes in different cell states have been predicted, important differentially expressed genes have been recovered, and new transition genes exclusive or shared between different cell state transitions have been predicted.
MOLECULAR SYSTEMS BIOLOGY
(2022)
Article
Environmental Sciences
Jiyu Li, Lu Zheng, Ye Fan, Yuerong Wang, Yurou Ma, Dongfang Gu, Yaping Lu, Shaoling Zhang, Xi Chen, Wei Zhang
Summary: PbMTP8.1 is a specific manganese transporter localized to the pre-vacuolar compartment, which enhances plant tolerance to manganese by sequestering manganese into vacuoles.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Chao Gu, Ruo-Fan Wu, Cai-Yun Yu, Kai-Jie Qi, Chen Wu, Hu-Ping Zhang, Shao-Ling Zhang
Summary: Sorbitol accumulation in pear fruit is cooperatively mediated by various SOTs, including SOT3, SOT6/20, SOT19/21, and SOT22. Sorbitol concentration showed positive correlations to fructose and glucose concentrations, indicating its role in determining fruit sweetness.
Article
Plant Sciences
Yun Wang, Guoming Wang, Wenyu Xu, Zhenwu Zhang, Xun Sun, Shaoling Zhang
Summary: This study found that exogenous melatonin treatment can enhance pear resistance to B. dothidea by increasing autophagic activity and the accumulation of soluble sugars and organic acids.
Article
Biotechnology & Applied Microbiology
Guoming Wang, Kaijie Qi, Xin Gao, Lei Guo, Peng Cao, Qionghou Li, Xin Qiao, Chao Gu, Shaoling Zhang
Summary: In this study, the evolution, characteristics, and expression patterns of PYL genes in Rosaceae plants were investigated. Whole-genome duplication and dispersed duplication were found to be the main drivers for the expansion of PYL gene family, and purifying selection was the major force in PYL gene evolution. qRT-PCR validation revealed the important roles of PYL genes in seed germination and abiotic stress responses in Chinese white pear.
Article
Agronomy
Hai-Nan Liu, Mao-Song Pei, Tong-Lu Wei, Yi-He Yu, Da-Long Guo
Summary: In this study, postharvest grapes treated with hypotaurine (HT) were found to delay the softening process and improve the quality of the fruit. The researchers identified differentially expressed genes and molecular pathways involved in this delay, shedding light on the molecular mechanisms of delaying berry softening.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Plant Sciences
Mao-Song Pei, Hai-Nan Liu, Charles Ampomah-Dwamena, Tong-Lu Wei, Yi-He Yu, Jia-Bing Jiao, Ying-Ying Lv, Feng Li, Hong-Chao Li, Xue-Jie Zhu, Da-Long Guo
Summary: This study has developed a simple and efficient transient transformation protocol for grape berries, which can be used as a valuable tool for the rapid testing of gene function and the study of key regulatory networks.
Article
Horticulture
Guoming Wang, Xin Gao, Xueping Wang, Peizhuo Liu, Sophia Lee Guan, Kaijie Qi, Shaoling Zhang, Chao Gu
Summary: This study investigates the formation process and regulatory mechanism of pear fruit size through measuring fruit characteristics, cell numbers, and transcriptome sequencing. The results suggest that fruit size is determined by both cell numbers and cell sizes, and differentially expressed genes related to pear fruit size have been identified.
SCIENTIA HORTICULTURAE
(2022)
Article
Plant Sciences
Guang-Qi He, Xi-Xi Huang, Mao-Song Pei, Hui-Ying Jin, Yi-Zhe Cheng, Tong-Lu Wei, Hai-Nan Liu, Yi-He Yu, Da-Long Guo
Article
Biochemistry & Molecular Biology
Guoming Wang, Lei Guo, Zhihua Guo, Sophia Lee Guan, Nan Zhu, Kaijie Qi, Chao Gu, Shaoling Zhang
Summary: In this study, the expression patterns of PbrEBF1, PbrEBF2, and PbrEBF3 in response to ethylene and 1-MCP treatment were investigated. PbrEBF3 showed potential association with fruit ripening and interaction with PbrEILs. PbrEBF3 plays a central role in pear fruit ripening through mediation of the ethylene signaling pathway.
Article
Agronomy
Mao -Song Pei, Hai-Nan Liu, Yi-He Yu, Tong-Lu Wei, Da-Long Guo
Summary: This study aimed to investigate the effect of folic acid (FA) treatment on the postharvest quality of table grape berries. The results showed that FA treatment reduced the rates of berry abscission, water loss, and berry softening, and decreased the accumulation of reactive oxygen species (ROS). Transcriptome analysis revealed differentially expressed genes (DEGs) between FA-treated and non-treated berries, and a hub transcription factor, VvWRKY31, was identified. The study provides insights into the potential molecular mechanism underlying FA-induced delay in postharvest quality deterioration of grape berries.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2023)
Article
Genetics & Heredity
Hai-Nan Liu, Mao-Song Pei, Charles Ampomah-Dwamena, Guang-Qi He, Tong-Lu Wei, Qiao-Fang Shi, Yi-He Yu, Da-Long Guo
Summary: This study assessed the distribution of long terminal repeat retrotransposons (LTR-RTs) in four cucurbit species and found that they played a significant role in the diversification of cucurbit species, especially in genome size variation. The study also revealed that LTR-RTs had an impact on genes related to dietary fiber synthesis and increased our understanding of genome evolution and trait characterization in cucurbits.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Agriculture, Multidisciplinary
Mao-Song Pei, Hai-Nan Liu, Tong-Lu Wei, Da-Long Guo
Summary: A 4D label-free quantitative proteomics approach was used to study non-histone lysine methylation during grape berry ripening. A total of 822 methylation sites in 416 methylated proteins were identified, with a conserved motif of xxExxx_K_xxxxxx. Functional annotation showed that these non-histone proteins with methylated lysine residues were mainly associated with ripening and senescence, energy metabolism, oxidation-reduction process, and stimulus response. The study also investigated the correlation of methylated proteins with QTLs, SNPs, and selective regions associated with fruit quality and development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Plant Sciences
Mao-Song Pei, Hai-Nan Liu, Tong-Lu Wei, Yi-He Yu, Da-Long Guo
Summary: This study extensively identified non-conventional peptides (NCPs) in grape using peptidogenomic methods. The NCPs were mainly derived from unannotated regions and had specific functions in grape berry development. These findings provide important insights for studying the functions of NCPs and discovering new functional genes in grape.
HORTICULTURE RESEARCH
(2022)
Article
Biology
Guoming Wang, Zhihua Guo, Xueping Wang, Sophia Lee Guan, Hongru Gao, Kaijie Qi, Chao Gu, Shaoling Zhang
Summary: In this study, 10 candidate reference genes were identified and evaluated for their expression stability during pear fruit development stages. The results showed that Pbr028511, Pbr038418, and Pbr041114 were the most stable reference genes in different pear cultivars. These findings provide valuable references for gene function investigations and molecular mechanism studies in fruit development and ripening for various pear cultivars.
Article
Biology
Hui-Ying Jin, Mao-Song Pei, Da-Long Guo
Summary: This study identified and analyzed the molecular characteristics of the grape miR477 gene family (Vvi-miR477) and predicted its target genes. The Vvi-miR477 family consists of two precursor sequences and three mature sequences, which are relatively conservative in sequence. The predicted target genes of Vvi-miR477 include 26S proteasome, DEAD-box, and other genes. In addition, the target genes are mainly involved in catalytic activity and carboxylic ester hydrolase activity according to the GO function enrichment analysis.