Review
Chemistry, Multidisciplinary
Minji Wang, Yifan Da, Yang Tian
Summary: Genetically encoded fluorescent sensors are powerful tools for visualizing physiological processes in living cells and animals. This study reviews the history and structural basis of fluorescent proteins and discusses the design of genetically encoded biosensors. Several major types of genetically encoded biosensors currently used are also briefly reviewed, providing insight for future fluorescent biosensor design.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Analytical
Simiao Zhang, Ning Zhang, Shishuang Wang, Zhenshun Li, Weiqing Sun, Mei Zhou, Yurong Zhang, Long Wu, Jing Ma
Summary: A highly sensitive fluorescence probe based on rhodamine 6G-loaded and MnO2 nanosheets-coated mesoporous silica nanospheres was developed for the detection of biogenic amines (BAs) in fish or fish products. The probe utilized R6G as an energy donor and MnO2 nanosheets as an energy acceptor. The method showed a wide linear detection range and high sensitivity, and was successfully validated using spiked fish samples, demonstrating its potential application in food safety.
MICROCHEMICAL JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Vera S. Ovechkina, Suren M. Zakian, Sergey P. Medvedev, Kamila R. Valetdinova
Summary: Genetically encoded fluorescent biosensors allow visualization of biological processes in real time, providing benefits such as monitoring drug action and screening at the single-cell resolution. Research aims to improve biomedical applications by exploring their potential and addressing challenges.
Review
Cell Biology
Hyunbin Kim, In-Yeop Baek, Jihye Seong
Summary: This review introduces genetically encoded fluorescent biosensors that can precisely monitor the real-time activation process of G protein-coupled receptors (GPCRs) in live cells. These sensors allow observation of the activation process of GPCRs, including the binding of extracellular ligands, conformational changes of GPCRs, recruitment of G proteins or beta-arrestin, internalization and trafficking of GPCRs, and downstream signaling events associated with GPCRs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan N. Haidey, Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Marie-Eve Paquet, Kaspar Podgorski, Mikhail Drobizhev, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell
Summary: L-Lactate, traditionally considered a metabolic waste product, is now recognized as an important intercellular energy currency in mammals. A genetically encoded biosensor called eLACCO1.1 has been developed to enable cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Fangfang Zhang, Anna B. Meier, Christine M. Poch, Qinghai Tian, Stefan Engelhardt, Daniel Sinnecker, Peter Lipp, Karl-Ludwig Laugwitz, Alessandra Moretti, Tatjana Dorn
Summary: Cardiomyocytes derived from hiPSCs are used as an excellent in vitro model in cardiovascular research, with changes in action potential dynamics being studied using high-throughput optical AP recordings. The study validated a voltage indicator hiPSC line expressing VSFP and established optical AP recordings in different subtypes and environments, expanding possibilities for non-invasive and long-term AP analysis in cardiovascular research and drug discovery.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Mehavesh K. Hameed, Javad B. M. Parambath, Sofian M. Kanan, Ahmed A. Mohamed
Summary: Biocompatible and luminescent nanostructures synthesized by capping gold-carbon nanoparticles with amino acids were successfully utilized for the quantitative estimation of the drug ranitidine. The method showed a detection limit of 0.174, 0.56, and 0.332 mu M for different amino acid bioconjugates, and was also successful in quantifying ranitidine in spiked serum samples. The study demonstrates the potential of this approach for sensitive and accurate drug quantification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Lan Mi, Qikun Yu, Aruni P. K. K. Karunanayake Mudiyanselage, Rigumula Wu, Zhining Sun, Ru Zheng, Kewei Ren, Mingxu You
Summary: RNA-based nanostructures and molecular devices have been widely used for biosensors and genetic regulators. However, the high reliance on fluorescence as the readout can cause issues such as high background and phototoxicity. In this study, we developed the first genetically encoded RNA-based bioluminescence resonance energy transfer (BRET) sensors for real-time target detection in living cells. This novel system has potential applications in bioanalysis and nanomedicine for engineering biosensors, characterizing cellular RNA-protein interactions, and high-throughput screening or in vivo imaging.
Article
Chemistry, Analytical
Xiaoke Bi, Connor Beck, Yiyang Gong
Summary: Genetically encoded fluorescent indicators, combined with optical imaging, have improved the detection of physiologically or behaviorally relevant neural activity with high spatiotemporal resolution. Protein engineering and screening strategies have enhanced the properties of genetically encoded fluorescent indicators, detecting neurotransmitter and calcium dynamics with high signal-to-noise ratio both in vitro and in vivo.
Review
Chemistry, Analytical
Hyunbin Kim, Jeongmin Ju, Hae Nim Lee, Hyeyeon Chun, Jihye Seong
Summary: Genetically encoded biosensors based on fluorescent proteins enable real-time monitoring of molecular dynamics, requiring different sensing strategies depending on the molecular events being monitored. Understanding principles and critical factors of each strategy is essential for successful development of new biosensors.
Article
Biochemistry & Molecular Biology
Oksana M. Subach, Aleksandr Tashkeev, Anna V. Vlaskina, Dmitry E. Petrenko, Filipp A. Gaivoronskii, Alena Y. Nikolaeva, Olga I. Ivashkina, Konstantin Anokhin, Vladimir O. Popov, Konstantin M. Boyko, Fedor Subach
Summary: Genetically encoded monomeric blue-to-red fluorescent timers can change their fluorescent color over time, and have important applications in cell research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jennifer Park, Michael B. Cleary, Danyang Li, Joseph A. Mattocks, Jiansong Xu, Huan Wang, Somshuvra Mukhopadhyay, Eric M. Gale, Joseph A. Cotruvo Jr
Summary: The design of selective metal-binding sites is challenging in both small-molecule and macromolecular chemistry. In this study, researchers successfully engineered lanmodulin into genetically encoded fluorescent sensors for selective detection of MnII. These sensors have potential applications in studying photosynthesis, host-pathogen interactions, and neurobiology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Chemistry, Analytical
Namdoo Kim, Seunghan Shin, Se Won Bae
Summary: cAMP serves a crucial role in signal transduction pathways, with research focusing on its dynamics leading to insights for drug development and disease treatment. To enable real-time non-invasive imaging, genetically-encoded sensors based on fluorescent proteins and luciferases have been developed as powerful tools.
Article
Biochemistry & Molecular Biology
Kristina R. Denisova, Nikita A. Orlov, Sergey A. Yakimov, Elena A. Kryukova, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Alexey V. Feofanov, Oksana V. Nekrasova
Summary: Peptide pore blockers and their fluorescent derivatives are important molecular probes for studying the Kv1.3 channel. GFP-MgTx, a fluorescent ligand based on green fluorescent protein (GFP) and margatoxin (MgTx), can recognize and evaluate Kv1.3 pore blockers of peptide origin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Liang Wang, Chunling Wu, Wanling Peng, Ziliang Zhou, Jianzhi Zeng, Xuelin Li, Yini Yang, Shuguang Yu, Ye Zou, Mian Huang, Chang Liu, Yefei Chen, Yi Li, Panpan Ti, Wenfeng Liu, Yufeng Gao, Wei Zheng, Haining Zhong, Shangbang Gao, Zhonghua Lu, Pei-Gen Ren, Ho Leung Ng, Jie He, Shoudeng Chen, Min Xu, Yulong Li, Jun Chu
Summary: In this study, a new fluorescent indicator called G-Flamp1 was developed to accurately measure levels of cAMP in living cells. G-Flamp1 exhibited good brightness, appropriate affinity, and fast response kinetics. The results showed that G-Flamp1 enables sensitive monitoring of endogenous cAMP signals in living organisms such as fruit flies and mice.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Suzan Youssef, Wei Ren, Hui-wang Ai
ACS CHEMICAL BIOLOGY
(2016)
Article
Chemistry, Analytical
Wei Ren, Ao Ji, Omran Karmach, David G. Carter, Manuela M. Martins-Green, Hui-Wang Ai
Article
Biochemistry & Molecular Biology
Yichong Fan, Merna Makar, Michael X. Wang, Hui-wang Ai
NATURE CHEMICAL BIOLOGY
(2017)
Article
Biochemical Research Methods
Hsien-Wei Yeh, Omran Karmach, Ao Ji, David Carter, Manuela M. Martins-Green, Hui-Wang Ai
Article
Biochemistry & Molecular Biology
Linfeng Gao, Xiao-Feng Tan, Shen Zhang, Tianchen Wu, Zhi-Min Zhang, Hui-wang Ai, Jikui Song
Article
Chemistry, Multidisciplinary
Ao Ji, Yichong Fan, Wei Ren, Shen Zhang, Hui-wang Ai
Article
Chemistry, Analytical
Hsien-Wei Yeh, Hui-Wang Ai
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 12
(2019)
Article
Biochemistry & Molecular Biology
Hsien-Wei Yeh, Tianchen Wu, Minghai Chen, Hui-wang Ai
Article
Biochemistry & Molecular Biology
Hsien-Wei Yeh, Ying Xiong, Tianchen Wu, Minghai Chen, Ao Ji, Xinyu Li, Hui-wang Ai
ACS CHEMICAL BIOLOGY
(2019)
Review
Biochemical Research Methods
Ying Xiong, Xiaodong Tian, Hui-wang Ai
BIOCONJUGATE CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Suzan Youssef, Shen Zhang, Hui-wang Ai
Article
Biochemistry & Molecular Biology
Alecia Marie Achimovich, Huiwang Ai, Andreas Gahlmann
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2019)
Article
Chemistry, Analytical
Minghai Chen, Shen Zhang, Yuan Xing, Xinyu Li, Yi He, Yong Wang, Jose Oberholzer, Hui-wang Ai
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Tianchen Wu, Yu Pang, Hui-wang Ai
Summary: The study investigated the tolerance of far-red FPs mMaroon1 and mCarmine towards circular permutation, resulting in the identification of five circularly permuted far-red FPs with excitation and emission maxima longer than 600 nm. Some of these variants showed appreciable brightness and efficient chromophore maturation, making them intriguing starting points for further engineering far-red GEFPIs for in vivo tissue imaging.
Review
Chemistry, Analytical
Zhijie Chen, Tan M. Truong, Hui-wang Ai