Steric Accessibility of the Cleavage Sites Dictates the Proteolytic Vulnerability of the Anti‐HIV‐1 Antibodies 2F5, 2G12, and PG9 in Plants

Commonality despite exceptional diversity in the baseline human antibody repertoire

(2019) Bryan Briney et al.   NATURE

Low Protease Content in Medicago truncatula Cell Cultures Facilitates Recombinant Protein Production

(2018) Rita B. Santos et al.   Biotechnology Journal

Structural analyses ofArabidopsis thalianalegumain γ reveal differential recognition and processing of proteolysis and ligation substrates

(2018) Florian B. Zauner et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in Nicotiana benthamiana

(2018) Friederike Grosse-Holz et al.   PLANT BIOTECHNOLOGY JOURNAL

Low Protease Content in Medicago truncatula Cell Cultures Facilitates Recombinant Protein Production

(2018) Rita B. Santos et al.   Biotechnology Journal

Combination therapy with anti-HIV-1 antibodies maintains viral suppression

(2018) Pilar Mendoza et al.   NATURE

Comparative Antigenicity of Thiourea and Adipic Amide Linked Neoglycoconjugates Containing Modified Oligomannose Epitopes for the Carbohydrate-Specific anti-HIV Antibody 2G12

(2018) Nino Trattnig et al.   BIOCONJUGATE CHEMISTRY

Two-faced Fcab prevents polymerization with VEGF and reveals thermodynamics and the 2.15 Å crystal structure of the complex

(2017) Elisabeth Lobner et al.   mAbs

From structure to function - a family portrait of plant subtilases

(2017) Andreas Schaller et al.   NEW PHYTOLOGIST

The transcriptome, extracellular proteome and active secretome of agroinfiltrated Nicotiana benthamiana uncover a large, diverse protease repertoire

(2017) Friederike Grosse-Holz et al.   PLANT BIOTECHNOLOGY JOURNAL

Nicotiana benthamiana cathepsin B displays distinct enzymatic features which differ from its human relative and aleurain-like protease

(2016) Melanie Niemer et al.   BIOCHIMIE

Site-targeted mutagenesis for stabilization of recombinant monoclonal antibody expressed in tobacco (Nicotiana tabacum) plants

(2016) Verena K. Hehle et al.   FASEB JOURNAL

CHO cell production and sequence improvement in the 13C6FR1 anti-Ebola antibody

(2016) Dean K. Pettit et al.   mAbs

Tackling Unwanted Proteolysis in Plant Production Hosts Used for Molecular Farming

(2016) Manoj K. Mandal et al.   Frontiers in Plant Science

Overcoming low yields of plant-made antibodies by a protein engineering approach

(2015) Julia Zischewski et al.   Biotechnology Journal

The increasing value of plant-made proteins

(2015) Markus Sack et al.   CURRENT OPINION IN BIOTECHNOLOGY

Substrate Specificity and Possible Heterologous Targets of Phytaspase, a Plant Cell Death Protease

(2015) Raisa A. Galiullina et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Viraemia suppressed in HIV-1-infected humans by broadly neutralizing antibody 3BNC117

(2015) Marina Caskey et al.   NATURE

Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants

(2015) Julian K-C. Ma et al.   PLANT BIOTECHNOLOGY JOURNAL

Glycan modulation and sulfoengineering of anti–HIV-1 monoclonal antibody PG9 in plants

(2015) Andreas Loos et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Overcoming low yields of plant-made antibodies by a protein engineering approach

(2015) Julia Zischewski et al.   Biotechnology Journal

Pharmacokinetics and Immunogenicity of Broadly Neutralizing HIV Monoclonal Antibodies in Macaques

(2015) Yvonne Rosenberg et al.   PLoS One

Inhibition of protease activity by antisense RNA improves recombinant protein production inNicotiana tabacumcv. Bright Yellow 2 (BY-2) suspension cells

(2014) Manoj K. Mandal et al.   Biotechnology Journal

The human anti-HIV antibodies 2F5, 2G12, and PG9 differ in their susceptibility to proteolytic degradation: Down-regulation of endogenous serine and cysteine proteinase activities could improve antibody production in plant-based expression platforms

(2014) Melanie Niemer et al.   Biotechnology Journal

Broadly Neutralizing Anti-HIV-1 Antibodies Require Fc Effector Functions for In Vivo Activity

(2014) Stylianos Bournazos et al.   CELL

Controlled glycosylation of plant-produced recombinant proteins

(2014) Richard Strasser et al.   CURRENT OPINION IN BIOTECHNOLOGY

Global Shape and Ligand Binding Efficiency of the HIV-1-neutralizing Antibodies Differ from Those of Antibodies That Cannot Neutralize HIV-1

(2014) Ashish K. Solanki et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies

(2014) Nicole A. Doria-Rose et al.   NATURE

Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase inNicotiana benthamiana

(2014) Jeannine D. Schneider et al.   PLANT BIOTECHNOLOGY JOURNAL

Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants

(2014) Verena K. Hehle et al.   PLANT BIOTECHNOLOGY JOURNAL

Proteolytic and N-Glycan Processing of Human  1-Antitrypsin Expressed in Nicotiana benthamiana

(2014) A. Castilho et al.   PLANT PHYSIOLOGY

Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco

(2013) Audrey Y-H. Teh et al.   PLANT BIOTECHNOLOGY JOURNAL

Antibody degradation in tobacco plants: a predominantly apoplastic process

(2011) Verena K Hehle et al.   BMC BIOTECHNOLOGY

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9

(2011) Jason S. McLellan et al.   NATURE

Broad neutralization coverage of HIV by multiple highly potent antibodies

(2011) Laura M. Walker et al.   NATURE

Increasing the Potency and Breadth of an HIV Antibody by Using Structure-Based Rational Design

(2011) R. Diskin et al.   SCIENCE

A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield

(2011) R. Pejchal et al.   SCIENCE

Fc-Glycosylation Influences Fc  Receptor Binding and Cell-Mediated Anti-HIV Activity of Monoclonal Antibody 2G12

(2010) D. N. Forthal et al.   JOURNAL OF IMMUNOLOGY

Antibody 2G12 Recognizes Di-Mannose Equivalently in Domain- and Nondomain-Exchanged Forms but Only Binds the HIV-1 Glycan Shield if Domain Exchanged

(2010) Katie J. Doores et al.   JOURNAL OF VIROLOGY

Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1

(2010) R. Pejchal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01

(2010) T. Zhou et al.   SCIENCE

Improved Virus Neutralization by Plant-produced Anti-HIV Antibodies with a Homogeneous β1,4-GalactosylatedN-Glycan Profile

(2009) Richard Strasser et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Design and Expression of a Dimeric Form of Human Immunodeficiency Virus Type 1 Antibody 2G12 with Increased Neutralization Potency

(2008) A. P. West et al.   JOURNAL OF VIROLOGY

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL

Cost-effective production of a vaginal protein microbicide to prevent HIV transmission

(2008) K. Ramessar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA