Overexpression of membrane proteins in mammalian cells for structural studies

Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells

(2012) Sarika Chaudhary et al.   Nature Protocols

Overproduction of human M3 muscarinic acetylcholine receptor: An approach toward structural studies

(2011) Wilber Romero-Fernandez et al.   BIOTECHNOLOGY PROGRESS

High throughput platforms for structural genomics of integral membrane proteins

(2011) Filippo Mancia et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The structural basis of agonist-induced activation in constitutively active rhodopsin

(2011) Jörg Standfuss et al.   NATURE

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II

(2011) X. Deupi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1

(2011) Karolina Corin et al.   Scientific Reports

High-throughput expression and purification of membrane proteins

(2010) Filippo Mancia et al.   JOURNAL OF STRUCTURAL BIOLOGY

Recent Advances in Lentiviral Vector Development and Applications

(2010) Janka Mátrai et al.   MOLECULAR THERAPY

Function of human Rh based on structure of RhCG at 2.1 A

(2010) F. Gruswitz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Functional expression of milligram quantities of the synthetic human serotonin transporter gene in a tetracycline-inducible HEK293 cell line

(2010) Hidehito Takayama et al.   PROTEIN EXPRESSION AND PURIFICATION

High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAAand serotonin receptors

(2010) Zuzana Dostalova et al.   PROTEIN SCIENCE

Structures of membrane proteins

(2010) Kutti R. Vinothkumar et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Location of the Retinal Chromophore in the Activated State of Rhodopsin*

(2009) Shivani Ahuja et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Thermostabilization of the Neurotensin Receptor NTS1

(2009) Yoko Shibata et al.   JOURNAL OF MOLECULAR BIOLOGY

Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4

(2009) B. L. Cook et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development and crystallization of a minimal thermostabilised G protein-coupled receptor

(2009) Tony Warne et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression, purification and in vitro functional reconstitution of the chemokine receptor CCR1

(2009) Samantha J. Allen et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression of glucagon receptors in tetracycline-inducible HEK293S GnT1− stable cell lines: An approach toward purification of receptor protein for structural studies

(2008) Cecilia G. Unson   BIOPOLYMERS

High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81

(2008) Hidehito Takayama et al.   PLoS One

Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line

(2008) Brian L. Cook et al.   PLoS One

Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

(2008) F. Magnani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conformational thermostabilization of the  1-adrenergic receptor in a detergent-resistant form

(2008) M. J. Serrano-Vega et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

(2008) Abhiram Dukkipati et al.   PROTEIN EXPRESSION AND PURIFICATION

Reflections on more than 10 years of TGE approaches

(2008) Sabine Geisse   PROTEIN EXPRESSION AND PURIFICATION