Article
Biology
Nicholas Sciolino, Anna Liu, Leonard Breindel, David S. Burz, Todd Sulchek, Alexander Shekhtman
Summary: This study demonstrates a new method for in-cell NMR using microfluidics to deliver labeled target proteins to cells for high-resolution structural studies. This technique does not affect cell viability and allows for long-term eukaryotic in-cell NMR experiments.
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Marti Checa, Ruben Millan-Solsona, Adrianna Glinkowska Mares, Silvia Pujals, Gabriel Gomila
Summary: Mapping the dielectric properties of cells with nanoscale spatial resolution is crucial for nanomedicine and nanotoxicity analysis, complementing structural and mechanical measurements. This study demonstrates the feasibility of obtaining dielectric constant maps of fixed cells in liquid environments, which helps preserve cell structure while accessing local dielectric properties under fully hydrated conditions. Further developments are needed to address the nanoscale dielectric imaging of living cells.
Article
Chemistry, Physical
Adria Botet-Carreras, M. Teresa Montero, Jesus Sot, Oscar Domenech, Jordi H. Borrell
Summary: The researchers engineered two different lipid model membranes mimicking the lipid composition of the HeLa cell plasma membrane, and studied the fusion process, finding a primary electrostatic interaction and confirming the enhancing effect of cholesterol. The results support the usefulness of the engineered model in the design of liposomes for drug delivery.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Chemistry, Multidisciplinary
Shu Zhang, Yamin Yang, Sijia Liu, Rui Dong, Zhiyu Qian
Summary: The paper examines the effects of high CO2 concentrations on tumor cell proliferation and metastasis, using a gas gradient-generating apparatus to study the response of Hela cells to different levels of CO2. The results show a significant reduction in cell viability with increasing CO2 concentration and incubation time, accompanied by intracellular acidification and elevated levels of reactive oxygen species.
Article
Nanoscience & Nanotechnology
Juan Carlos Camacho-Fernandez, Genesis Karendash Gonzalez-Quijano, Childerick Severac, Etienne Dague, Veronique Gigoux, Jaime Santoyo-Salazar, Adrian Martinez-Rivas
Summary: The study investigated the impact of magnetic nanoparticles interacting with HeLa cells under high frequency alternating magnetic field. It was found that these interactions modified the biomechanical behavior of the cell interfaces, particularly with Fe3O4@SiO2-NH2 MNPs incubated HeLa cells exposed to AMF showing the most significant difference in elasticity and stiffness.
Article
Oncology
Iwan Setiawan, Ronny Lesmana, Hanna Goenawan, Dodi Suardi, Vesara Ardhe Gatera, Rizky Abdulah, Raden Tina Dewi Judistiani, Unang Supratman, Budi Setiabudiawan
Summary: This study evaluated the effect of Calcitriol on autophagy and mitochondria homeostasis in HeLa cells and found that Calcitriol can induce cell death by activating the PI3K-AKT-mTOR pathway and inhibiting autophagy.
JOURNAL OF CANCER RESEARCH AND THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Juan Alcalde, Mads Munk, Maria Gonzalez-Munoz, Svetlana Panina, Martin W. Berchtold, Antonio Villalobo
Summary: In this study, a novel genetic system using CRISPR/Cas9-mediated gene deletion and conditional CaM expression was introduced to investigate the function of CaM in HeLa cells. The results showed that CaM downregulation inhibited both 2D and 3D migration in HeLa cells, with an impact on 2D migration in the presence of EGF. Additionally, downregulation of CaM decreased the expression of Racl, Cdc42, and RhoA, known to play crucial roles in cell migration.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Environmental Sciences
Yuming Zhang, Fengyu Yuan, Pei Li, Jihai Gu, Junjun Han, Zhihua Ni, Fengsong Liu
Summary: This study found that Resveratrol (RES) inhibits the proliferation of HeLa cells by perturbing mitochondrial structure and function, and ROS-induced autophagy plays a critical role in this process.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Microbiology
Maram Sbehat, Mohammad Altamimi, Mohammad Sabbah, Gianluigi Mauriello
Summary: Probiotics were coated using a layer-by-layer technique with black seed protein as a coating material, and the viability of coated Lacticaseibacillus rhamnosus was significantly improved. The use of inulin and black seed protein as coating materials showed the best protection for L. rhamnosus under high temperatures. In addition, edible films prepared with coated cells showed better physical properties compared to films without coating.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Bu Liu, Jielun Hu, Haodong Yao, Lin Zhang, Huan Liu
Summary: The aim of this study was to encapsulate probiotics in zein nanoparticles and pectin using layer-by-layer assembly, and investigate their influences on cell viabilities during various processes. High survival rates were achieved (>95%) during fabrication. Increasing the number of coating layers enhanced surface structure density, but showed poor correlation with cell viabilities during heating and storage. Pectin as the outermost layer promoted viability during heating, while ZNP was more beneficial during storage. Insufficient coating layers during gastrointestinal digestion exposed cells to harsh conditions faster, but sufficient coating offset this adverse impact. Hence, the matrix of the outermost layer and the number of coating layers played a crucial role in cell viabilities to external adverse conditions for LbL assembled probiotic microcapsules.
FOOD HYDROCOLLOIDS
(2023)
Article
Food Science & Technology
Xiaoqiang Chen, Ke Zhang, Yan 'an Wei, Yongyong Wang, Xiaofan Wu, Haifeng Wan
Summary: Adverse environments like the gastrointestinal tract can affect the effectiveness of probiotics, so protecting and fully utilizing their effects are important in probiotic research. In this study, green tea polysaccharide conjugate (gTPC) and gelatin were used as encapsulation materials for Lactobacillus acidophilus, and layer-by-layer self-assembly technology was applied. The results showed that increasing the number of deposited layers improved the encapsulation and protection of the probiotics, enhancing their survival rate.
Article
Chemistry, Physical
Xian-Gang Hu, Qinwei Wei, Yi-Ming Zhao, Peng-Xiang Hou, Wencai Ren, Chang Liu, Hui-Ming Cheng
Summary: Using a simple drop-casting method, a FeCl3-functionalized GO/SWCNT composite film was fabricated for heterojunction solar cells, which significantly increased the photocurrent, photovoltage, and fill factor, resulting in a high conversion efficiency of 17.5%.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Article
Biochemical Research Methods
Lisa Wehl, Constantin von Schirnding, Marie C. Bayer, Olga Zhuzhgova, Hanna Engelke, Thomas Bein
Summary: This study successfully synthesized biocompatible and biodegradable amorphous mesoporous magnesium phosphate-citrate nanoparticles (MPCs), which can serve as a drug delivery system with the potential to enhance the effectiveness of anticancer drugs. In vitro experiments demonstrated their ability to reduce premature release and efficiently release drugs in a stimuli-responsive manner, suggesting their potential for reducing the side effects of current chemotherapies.
BIOCONJUGATE CHEMISTRY
(2022)
Review
Crystallography
Silvia Maria Cristina Rotondi, Giorgia Ailuno, Simone Luca Mattioli, Alessandra Pesce, Ornella Cavalleri, Paolo Canepa
Summary: In this review, we discuss the progress in investigating macromolecular crystals using atomic force microscopy (AFM), a powerful tool for high-resolution imaging of surfaces and specimens. AFM enables visualization of soft samples at the nanoscale, providing detailed visual details from the molecular to micrometer range. AFM is well-suited for studying fragile samples like macromolecular crystals due to its nonperturbative nature, ability to scan in liquid environments, and no need for freezing or staining. This review covers the achievements of AFM in understanding crystal growth processes at both micro- and nanoscale, analyzes its capability to investigate sample structure at the single molecular level, and discusses the evolution of high-speed AFM to overcome the limitations of low imaging speed and observe molecular dynamics.
Article
Oncology
Yang Yu, Yanwen Li, Xinjie Yang, Qiuyi Deng, Bin Xu, Hua Cao, Jianwen Mao
Summary: This study designed and synthesized a series of novel imidazo[1,2-a]pyridine-derived compounds. The compound La23 showed high potential for suppressing the viability of HeLa cells by inducing cell apoptosis. The results suggest that La23 has potential antitumor effects.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Food Science & Technology
Jose C. Bonilla, Victor Bernal-Crespo, James A. Schaber, Arun K. Bhunia, Jozef L. Kokini
FOOD RESEARCH INTERNATIONAL
(2019)
Article
Engineering, Chemical
Nathalia Olivera, Tahrima B. Rouf, Jose C. Bonilla, Jose G. Carriazo, Nathania Dianda, Jozef L. Kokini
JOURNAL OF FOOD ENGINEERING
(2019)
Article
Materials Science, Multidisciplinary
Hazal Turasan, Mukerrem Cakmak, Jozef Kokini
JOURNAL OF MATERIALS SCIENCE
(2019)
Article
Agriculture, Multidisciplinary
Fei Jia, Emma Barber, Hazal Turasan, Sujin Seo, Ruitong Dai, Logan Liu, Xingmin Li, Arun K. Bhunia, Jozef L. Kokini
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Engineering, Chemical
Tuncay Yilmaz, Luis Maldonado, Hazal Turasan, Jozef Kokini
JOURNAL OF FOOD ENGINEERING
(2019)
Article
Chemistry, Applied
Xingfei Li, Luis Maldonado, Morgan Malmr, Tahrima B. Rouf, Yufei Hua, Jozef Kokini
FOOD HYDROCOLLOIDS
(2019)
Article
Food Science & Technology
Secil Turksoy, Merve Y. Erturk, Jose Bonilla, Hazal Turasan, Jozef L. Kokini
JOURNAL OF CEREAL SCIENCE
(2020)
Article
Food Science & Technology
Jose C. Bonilla, Merve Y. Erturk, James A. Schaber, Jozef L. Kokini
JOURNAL OF CEREAL SCIENCE
(2020)
Review
Engineering, Chemical
Hazal Turasan, Jose Bonilla, Fatih Bozkurt, Luis Maldonado, Xingfei Li, Tuncay Yilmaz, Rohollah Sadeghi, Jozef Kokini
JOURNAL OF FOOD PROCESS ENGINEERING
(2020)
Article
Chemistry, Applied
Merve Yildirim Erturk, Jose C. Bonilla, Jozef Kokini
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Applied
Jose C. Bonilla, Mathias P. Clausen
Summary: Modern research on food materials has advanced in characterizing them at both the molecular and macroscopic levels, yet there remains a gap in understanding their microscopic organization. Utilizing optical superresolution microscopy, this study shows how more detailed information about food microstructures can be obtained compared to conventional microscopy. By studying protein aggregation in egg-white using STED microscopy, it was found to provide a 5-fold increase in resolution and allowed for a more precise quantification of structures compared to confocal microscopy. This additional information from STED microscopy could be correlated with macroscopic rheological measurements to provide a more complete understanding of protein aggregation in food matrices.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Jose C. Bonilla, Jesper L. Sorensen, Amalie S. Warming, Mathias P. Clausen
Summary: Recent advances in software development and machine learning algorithms have revolutionized the analysis and quantification of microstructures in fields like biology and neuroscience. This article demonstrates the use of a deep-learning cellular segmentation algorithm, 'Cellpose', to identify and quantify foam microstructures for the study of food structure-function relationships. The algorithm successfully identified air bubbles in a protein foam matrix and allowed for further analysis of microstructural parameters. The study examined the effects of sugar concentration and acidic conditions on the microstructural mechanisms and rheological responses of the foam. The understanding gained from the algorithm-identified microstructures provides a new approach to characterize and study food foams.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Jose C. Bonilla, Merve Y. Erturk, Jozef L. Kokini
FOOD HYDROCOLLOIDS
(2020)
Article
Chemistry, Applied
Deepika Sharma, Gregory R. Ziegler, Federico M. Harte
Summary: Fabrication of bead-free nanofibers from pure casein is challenging due to its self-aggregation tendency. In this study, the influence of pH, ethanol content, ionic environment, and casein concentration on the solubility and solution characteristics of casein-based spinning dopes was analyzed. The addition of tetrasodium pyrophosphate (TSPP) reduced bead defects and improved the properties of the nanofibers. The viscosity of the solution depended on the casein concentration, and bead-free nanofibers were obtained at a concentration higher than the entanglement concentration (C-e).
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Smriti Shrestha, Leonie Van't Hag, Victoria Haritos, Sushil Dhital
Summary: This study investigated the pasting properties, structural formation, and molecular changes of mungbean, green lentil, and yellow pea proteins during high moisture extrusion (HME). The results showed that protein composition and conformation played important roles in the structural development and molecular changes during HME.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Shi Liu, Na Sun, Kunyu Ren, Xubin Tan, Lanxin Li, Zhe Wang, Shicheng Dai, Xiaohong Tong, Huan Wang, Lianzhou Jiang
Summary: In this study, self-assembled soy protein nanoparticles (SPN) were successfully prepared and used for encapsulation of lutein, 8-carotene, and curcumin. The encapsulated natural pigments showed improved stability, especially curcumin. This study provides a new approach for extending the use of lipophilic natural edible pigments in food products.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Jianming Wang, Ziyun Liu, Kaiwen Zheng, Zhe Yuan, Chen Yang
Summary: By combining with high methoxyl pectin (HMP), the emulsifying properties of oat protein isolate (OPI) were improved. The OPI-HMP complex formed soluble complexes under specific pH conditions, exhibiting excellent emulsifying activity and stability, making it suitable as a delivery system for lipid soluble bioactive compounds in food and biomedical applications.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Zihao Yin, Junzhe Zou, Mengwei Wang, Ruonan Huang, Yuemiao Qian, Mingyong Zeng, Fangwei Li
Summary: Phycocyanin, a protein prone to degradation under acidic conditions and unstable at high temperatures, has limited application in food. This study successfully combined phycocyanin with whey protein isolate using protein coprecipitation and chitosan coating to form composite particles, which maintained the color and colloidal stability of phycocyanin. The composite particles showed minimal color changes and retained high pigment retention rates after heating. Furthermore, the composite particles exhibited excellent antioxidant capacity. This breakthrough expands the application of phycocyanin in acidic beverages and other food forms.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Yuting Su, Qian Zhou, Hui Xu, Manting Huang, Siqian Li, Jiayi He, Ka-Wing Cheng, Mingfu Wang
Summary: By developing the CM-Cur/QT complex, the solubility and stability of quercetin were improved, leading to enhanced bioavailability. The complex showed increased bio-accessibility and antioxidant efficacy during in vitro digestion, and facilitated the internalization of quercetin in macrophages, exerting a potential synergistic anti-inflammatory effect.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Sheliang Zhao, David Julian Mcclements, Xuebo Liu, Fuguo Liu
Summary: The low oral bioavailability of some bioactive ingredients is a challenge in the supplement and functional food areas. This study designs natural carriers that can simultaneously deliver both hydrophobic and hydrophilic bioactive ingredients, thereby increasing their ability to exert synergistic effects.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Md Easdani, Shabbir Ahammed, Md Nazmus Saqib, Fei Liu, Fang Zhong
Summary: Compiling hydrophobic and hydrophilic proteins to form a mechanically reliable bilayer film with improved water barrier properties and hydrophobicity is challenging. By introducing cross-linkers, the film exhibits compatible and flexible surfaces, enhanced color and transparency, and controlled water vapor permeability. The bilayer film shows potential for improving value-added functional properties.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Tian-Qi Zhou, Xue-Chen Wang, Ling-Yi Gao, Jia-Nan Yan, Hai-Tao Wu
Summary: This study investigated the synergetic gelation of curdlan gum (CG) and gellan gum (GG) at different ratios. The optimal gelation was achieved at a mass ratio of 1:1 and a total concentration of 0.6%. The gel properties were influenced by the CG/GG ratio, with the strongest gel observed at a ratio of 5:5. The improved gel properties were attributed to hydrogen bonds between CG and GG. The CG/GG binary composite gel exhibited increased water holding capacity as the CG/GG ratio decreased. Furthermore, the microstructure of the CG/GG gel was denser and more homogeneous, supporting its stronger gel rigidity and water holding capacity.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Jialin Sun, Zihao Wei, Changhu Xue
Summary: In this study, porous gelatin microspheres (PGMs) with surface and internal porous structure were developed for the controlled release and rapid functional response of anthocyanins (ANCs). The loading of ACNs was achieved through electrostatic interaction, resulting in a high loading capacity. ACN@PGMs exhibited long-term controlled release, improved storage stability, and ammonia responsiveness, making them suitable for applications as antioxidant factors and food freshness indicators in the food industry.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Xinlai Dou, Yanling Hao, Ying Sun, Pin Yang, Linlin Liu, Yinyuan He, Yanguo Shi, Chunhua Yang, Fenglian Chen
Summary: In this study, chitosan hydrochloride/carboxymethyl starch sodium (CHC-CMS-Na) nanogels were prepared as a baking additive and showed potential in improving bread quality.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Xinya Wang, H. Douglas Goff, Steve W. Cui
Summary: This study thoroughly investigated the synergistic gelation between yellow mustard gum (YMG) and kappa-carrageenan using multiple techniques. The results showed that the interaction between YMG and kappa-carrageenan was mainly polymer associations, and YMG affected the gel formation process of kappa-carrageenan.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Jielong Zhang, Dasong Liu, Xiumei Tao, Jun Tang, Xiaoyu Peng, Thom Huppertz, Xiaoming Liu, Peng Zhou
Summary: The effect of enzymatic dephosphorylation on the structure of casein micelles in caprine micellar casein concentrate was studied. The results showed that with increasing dephosphorylation, the molar mass decreased, the radii decreased, the hydration increased, and the internal protein inhomogeneity disappeared.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Wentao Ma, Chao Yuan, Bo Cui, Tian Gao, Li Guo, Bin Yu, Meng Zhao, Feixue Zou
Summary: This study investigated the influence of highly-branched cyclic dextrin (HBCD) on the physiochemical properties of x-carrageenan (KC) gels. The addition of HBCD significantly enhanced the storage modulus and freeze-thaw stability of KC gels. HBCD promoted the distribution of KC chains and aggregation of KC helixes, resulting in a compact network structure and reduced water loss. Furthermore, HBCD strengthened the thermal reversibility of KC gels through enhanced hydrogen-bonding interactions.
FOOD HYDROCOLLOIDS
(2024)
Article
Chemistry, Applied
Ya-Ru Wang, Qin Yang, Yi-Xuan Jiang, Han-Qing Chen
Summary: This study successfully improved the aqueous solubility, thermal stability, and antioxidant activity of RES by using ovalbumin fibrils as nanocarriers. The pH value was found to have an impact on the formation and properties of the complex, with the best interaction observed at pH 2.0.
FOOD HYDROCOLLOIDS
(2024)