Article
Chemistry, Analytical
Bingzhi Li, Anqi Xia, Siying Xie, Lei Lin, Zhirun Ji, Tiying Suo, Xing Zhang, He Huang
Summary: A signal-amplified detection method of FEN1 based on cleavage-induced ligation and rolling circle amplification was developed in this study, achieving highly sensitive and specific detection of FEN1 in real samples, as well as evaluating the inhibitory effects of chemicals on FEN1. This novel biosensor method enriches the tools for FEN1-based diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yingli Han, Yuehua Wang, Fangyu Zhou, Yuyang Jiang, Feng Liu
Summary: This article introduces a new tetrahedral molecular beacon (TMB) probe for the detection of APE 1 activity. The probe has the advantages of being able to enter cells, simple operation, and low cost. The extracellular detection limit of the probe is very low and it has successfully distinguished cancer cells overexpressing APE 1 from normal cells. Additionally, the probe can be used as an effective means for screening APE 1 inhibitors and monitoring the response of APE 1 to different drugs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Inorganic & Nuclear
Ratish R. Nair, Jong Min An, Jaehoon Kim, Dokyoung Kim
Summary: The use of biofluids as a diagnostic tool for chronic health conditions has gained popularity in recent years. These fluids can be obtained non-invasively or invasively, offering a convenient and rapid means of diagnosis.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Chen Wang, Di Hao, Yaofei Bao, Xiaoxiao Xu, Yifan Li, Bingjie Zou, Li Ding, Qinxin Song
Summary: In this study, a nano firework was introduced as a fluorescent sensor to detect and report changes in FEN1 activity in living cells. The nano firework showed high selectivity, anti-interference ability, stability, and quantitative performance in both tubes and living cells. It accurately reported changes in FEN1 activity in different cells and could be used for high-throughput screening applications. Additionally, two new candidate compounds, myricetrin and neoisoliquritin, were discovered as potential FEN1 inhibitors for further research.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Aijiao Yuan, Hang Xiao, Fan Yang, Huifang Hao, Xiaoyan Wang, Jinhua Li, Minghua Jin, Qiang Zhao, Rui Sha, Zhaojia Deng, Hanyong Peng
Summary: The rapid development of DNA nanotechnology allows for the programmable operation of advanced DNA walkers, which mimic the function of protein motors in living organisms. These walkers require four main components for construction, including motor, track, energy, and cargo, and can autonomously perform specific functions following a predesigned trajectory with nanometer precision. Recent research has focused on improving the progressivity, speed, and directionality of DNA walkers, and new DNA nano techniques have shown powerful signal amplification during their operation. This review covers the breakthrough design and principle of walking systems and highlights the recent application of DNA walkers for signal amplification in living cells. The engineered DNA walkers have great potential for diverse applications, such as imaging intracellular molecules, modulating cellular functions, and facilitating drug delivery.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Han Zhou, Xin Du, Zhenguo Zhang
Summary: The increasing incidence and mortality of cancer in recent years have driven the development of accurate and rapid early diagnosis methods. Electrochemical cytosensors have attracted wide attention in tumor cell detection due to their high sensitivity and low cost.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Mingtai Sun, Tian Wang, Xin Yang, Huan Yu, Suhua Wang, Dejian Huang
Summary: A new fluorescent probe BV-1 based on BODIPY group was synthesized and demonstrated to detect viscosity in living cells with high selectivity and sensitivity. The probe showed significant enhancement in fluorescence with redshift in emission wavelength under high viscosity conditions, providing efficient ways for the development of viscosity probes and monitoring of intracellular viscosity properties and functions.
Article
Spectroscopy
Boxin Zhou, Beibei Wang, Mengqi Bai, Mingdong Dong, Xu Tang
Summary: Cysteine plays an important role in the human body, and abnormal concentration can lead to various diseases. Detecting cysteine in vivo with high selectivity and sensitivity is therefore significant. In this study, a small organic fluorescent probe ZHJ-X was designed and synthesized based on cyanobiphenyl, which specifically recognized cysteine. The probe ZHJ-X demonstrated specific selectivity, high sensitivity, short response time, good anti-interference ability, and a low detection limit of 3.8 x 10-6 M. It was successfully applied for visualizing cysteine in living cells and holds potential for cell imaging and detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biophysics
Yuhua Chen, Yuxin Xing, Zhenqiang Wang, Lin Li, Hailing Wang, Shuqi Tang, Kaiyong Cai, Jixi Zhang
Summary: A dual-factor coactivation strategy was developed to enhance tumor imaging by increasing the accessibility of cytosolic biomarkers. Application of a lysosomotropic agent facilitated the release of nanosensors, leading to a significant increase in intracellular fluorescence in vitro. The coactivation strategy also improved the cancer-to-normal cell signal ratio and resulted in high tumor-to-normal tissue ratio values in the in vivo imaging results. This strategy establishes a new paradigm for selective and specific tumor imaging using fluorescent nanosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Materials Science, Multidisciplinary
Fengying Qin, Juanyan Wu, Dongmei Fu, Ye Feng, Chao Gao, Dazhi Xie, Shaoming Fu, Suyi Liu, Daniela A. Wilson, Fei Peng
Summary: The study proposes a DNA detection system based on a magnetic helical hydrogel micromotor, which can detect tumor DNA in a non-invasive, quick, and simple manner, exhibiting excellent moving performance and high efficiency response.
APPLIED MATERIALS TODAY
(2022)
Article
Chemistry, Analytical
Qi Dai, Xiaojuan Wang, Cai Liu, Zhenzhen Feng, Baosheng Ge, Xiqi Ma, Zhixiong Zhang, Jinyi Yu, Xiaoqiang Wang, Fang Huang
Summary: A novel fluorescent probe ECDY was developed in this study to sensitively detect tyrosinase activity in melanoma cells through specific recognition of tyrosine groups. Experimental results showed that ECDY accurately measured tyrosinase activity in different cell lines and specifically detected melanoma cell lysate. Confocal fluorescence imaging experiments further demonstrated ECDY's ability to significantly distinguish melanoma cells from others.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Kunhan Nie, Yongjian Jiang, Na Wang, Yajun Wang, Di Li, Lei Zhan, Chengzhi Huang, Chunmei Li
Summary: The study proposes a programmable DNAzyme amplifier strategy for the simple, rapid, and universal detection of pancreatic cancer-related miRNAs. Through rational sequence design, the method demonstrates high selectivity and sensitivity, with potential for wide application.
Article
Chemistry, Analytical
Jing Lei, Lu Shi, Wei Liu, Baoxin Li, Yan Jin
Summary: A simple and sensitive chemiluminescence method using a handheld luminometer was proposed for portable detection of cancer cells. The method utilizes a cyclic strand displacement reaction for signal amplification and achieves sensitive detection of cancer cells with a low detection limit. This provides a new option for the early diagnosis of cancers.
Article
Chemistry, Analytical
Longyang Yang, Yinxiang Xu, Junming Dong, Yadong Lu, Hai-Liang Zhu, Zhen Li
Summary: A novel fluorescent probe, GTP, was developed for monitoring the GGT level in living cells and biopsies. It showed a ratio response between the signal intensity at 560 nm and 500 nm (RI560/I500), with a linear range of 0-50 U/L and a detection limit of 0.23 mu M. GTP exhibited high selectivity, good anti-interference, and low cytotoxicity, making it suitable for physiological applications. By monitoring the GGT level using ratio values in the green and blue channels, GTP could distinguish cancer cells and tumor tissues from normal cells and tissues.
Article
Chemistry, Multidisciplinary
Yousef Bagheri, Ahsan Ausaf Ali, Puspam Keshri, James Chambers, Anne Gershenson, Mingxu You
Summary: A new DNA-based probe called DNA Zipper was introduced in this study, which enables imaging of membrane order and transient interactions in living cells. These programmable DNA probes, by fine-tuning DNA hybridization, can precisely extend the duration of membrane lipid interactions and have been applied to study the correlation between membrane order and T-cell receptor signaling activation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Analytical
Xue-Nan Feng, Yun-Xi Cui, Jing Zhang, An-Na Tang, Han-Bin Mao, De-Ming Kong
ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Sagun Jonchhe, Shankar Pandey, Deepak Karna, Pravin Pokhrel, Yunxi Cui, Shubham Mishra, Hiroshi Sugiyama, Masayuki Endo, Hanbin Mao
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Yun-Xi Cui, Guo-Ying Sun, Yong-Hui Li, An-Na Tang, Li-Na Zhu, De-Ming Kong
ADVANCED MATERIALS INTERFACES
(2020)
Article
Biochemical Research Methods
Deepak Karna, Morgan Stilgenbauer, Sagun Jonchhe, Kazuya Ankai, Ibuki Kawamata, Yunxi Cui, Yao-Rong Zheng, Yuki Suzuki, Hanbin Mao
Summary: By utilizing DNA origami nanosprings, the clustering of integrin molecules can be controlled under different pH conditions to modulate cell motions. The nanospring coils and inhibits cell movement under acidic conditions, while it uncoils and allows cells to move under neutral conditions.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yujia Wang, Hao Zhang, Yunxi Cui, Shaoxiang Duan, Wei Lin, Bo Liu
Summary: A biocompatible pH biosensor based on a DNA-functionalized microfiber-assisted Mach-Zehnder interferometer was proposed, utilizing i-motif and complementary DNA enhancement mechanism for improved sensitivity. Experimental results showed stable performance with wide pH range and high sensitivity, making it suitable for physiological applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Ya-Xin Wang, Dong-Xia Wang, Jing Wang, Yi-Chen Du, Yun-Xi Cui, An-Na Tang, Hong-Xin Jiang, De-Ming Kong
Summary: By controlling the assembly/disassembly of DNA tetrahedron, three binary INHIBIT logic gates were constructed, and various sensing systems were successfully developed for the ratiometric detection of microRNA, Hg2+, cysteine and pH value. The proposed sensing systems demonstrated efficient internalization of DNA nanostructures by cells for ratiometric imaging of microRNA, Hg2+ and pH in living cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Qiong Wang, Jing Wang, Yan Huang, Yichen Du, Yi Zhang, Yunxi Cui, De-ming Kong
Summary: Molecular biomarkers are crucial for disease diagnosis and biological research, with DNA-based biosensors emerging as versatile tools for their detection. The development of highly efficient probes for biomarker detection is an attractive research field, especially in the context of urgent demands for improved biosensor performance in clinical applications. The rapid and sensitive detection methods developed in response to the COVID-19 pandemic showcase the importance of DNA-based biosensors in advancing disease diagnosis and treatment.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Multidisciplinary
Jing Wang, Dong-Xia Wang, Bo Liu, Xiao Jing, Dan-Ye Chen, An-Na Tang, Yun-Xi Cui, De-Ming Kong
Summary: DNA nanotechnology utilizes the unique properties and biocompatibility of DNA to construct structurally-defined supramolecular structures, and it has been widely applied in the field of biosensors. With the continuous development of technology, the assembly methods of DNA nanostructures have become more diverse. This review summarizes the latest construction methods and discusses the problems and future directions in this field.
CHEMISTRY-AN ASIAN JOURNAL
(2022)
Article
Cell Biology
Fan Yu, Qianping Zhang, Hanyu Liu, Jinming Liu, Song Yang, Xiaofan Luo, Wei Liu, Hao Zheng, Qiqi Liu, Yunxi Cui, Guo Chen, Yanjun Li, Xinglu Huang, Xiyun Yan, Jun Zhou, Quan Chen
Summary: The O-GlcNAcylation of proteins plays a role in regulating ferroptosis by modulating ferritinophagy and mitophagy. Inhibition of O-GlcNAcylation promotes ferritinophagy and enhances mitophagy, resulting in the accumulation of labile iron and increased sensitivity to ferroptosis.
Article
Materials Science, Paper & Wood
Peixuan Wu, Yunxi Cui, Zhicheng Sun, Meijuan Cao, Yuanyuan Liu, Kun Fu, Yang Zhou
Summary: In this study, an antibacterial sodium alginate derivative was successfully developed through covalent coupling of organosilane quaternary ammonium salt with sodium alginate. The material exhibited high antibacterial efficiency against Staphylococcus aureus and Escherichia coli, reaching up to 99.6% and 99.3%, respectively. Additionally, the material demonstrated good biocompatibility and potential application in the fabrication of sodium alginate beads.
Article
Chemistry, Multidisciplinary
Dong-Xia Wang, Jing Wang, Ya-Xin Wang, Jia-Yi Ma, Bo Liu, An-Na Tang, De-Ming Kong
Summary: The separation and detection of circulating tumor cells (CTCs) have a significant impact on clinical diagnosis and treatment. This study presents a strategy using a dual-aptamer DNA network to specifically capture and controllably release CTCs, allowing downstream analysis of live cells. The programmability of CRISPR/Cas12a is utilized to activate the responsive release of captured CTCs. This strategy provides a powerful and effective method for capturing and releasing CTCs from complex physiological samples.
Article
Chemistry, Multidisciplinary
Dong-Xia Wang, Ya-Xin Wang, Jing Wang, Jia-Yi Ma, Bo Liu, An-Na Tang, De-Ming Kong
Summary: This study demonstrates that MnO2 nanosheets are an excellent carrier for the CRISPR/Cas12a system, enabling efficient delivery and rapid release in living cells. The use of MnO2 nanosheets improves the performance of live-cell biosensing analysis compared to commercial carriers.
Review
Chemistry, Multidisciplinary
Jia-Yi Ma, Ya-Xin Wang, Yan Huang, Yi Zhang, Yun-Xi Cui, De-Ming Kong
Summary: Cell-cell aggregation is a well-known mode of intercellular communication that plays a vital role in multicellularity and organism development. In recent decades, scientists in biology and bioengineering have shown great interest in understanding the bioprocesses and molecular mechanisms related to cell-cell aggregation. They have developed various strategies to artificially regulate cell-cell aggregation using chemical-biological approaches. This review discusses the recent advances in manipulating cell-cell aggregation, including the use of chemical reactions and biological conjugations, and explores their advantages and potential applications. Additionally, the challenges and future perspectives for cell manipulation and cell-based therapy are proposed. This review aims to inspire innovative work in the field of cell-cell aggregation manipulation and cell-cell interactions in bio/chemical research.
Review
Chemistry, Multidisciplinary
Dong-Xia Wang, Jing Wang, Ya-Xin Wang, Yi-Chen Du, Yan Huang, An-Na Tang, Yun-Xi Cui, De-Ming Kong
Summary: DNA nanostructure-based probes have shown advantages such as enhanced cell internalization efficiency and accelerated reaction rate, thus improving the efficiency of applications in biosensing, imaging analysis, and cell assembly.
