Article
Chemistry, Analytical
Pian Wu, Man Zhang, Xiaoting Xue, Ping Ding, Lei Ye
Summary: A novel dual-amplification system based on CRISPR-Cas12a and horseradish peroxidase (HRP) was developed for colorimetric determination of MC-LR. The system combines the nuclease activity of CRISPR-Cas12a with the redox activity of HRP. The dual-signal amplification enables the detection of MC-LR in a range of 0.01 to 50 ng·mL(-1), with a limit of detection of 4.53 pg·mL(-1).
Article
Chemistry, Multidisciplinary
Guangming Li, Hao Liu, Tianding Hu, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study reports a dimensionality engineering strategy to convert conventional 2D Fe-N-4 centers into 3D structures by integrating oxidized sulfur functionalities onto the carbon plane. The results suggest that oxidized sulfur functionalities can serve as binding sites for assisting substrate orientation and facilitating the desorption of H2O, resulting in an outstanding specific activity of up to 119.77 U mg(-1), which is 6.8 times higher than that of conventional FeN4C SAzymes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Yue He, Jing Zhou, Ruijie Fu, Yanlin Liu, Yiwen Wang, Haoran Liu, Jing Zhao, Yongliang Cui, Bining Jiao
Summary: An ultrasensitive colorimetric method for detecting genomic DNA of Alternaria from citrus fruit samples was described in this work, utilizing a system of five groups of reporter probes that form a DNA-heptamer sandwich structure on magnetic beads. The method has a low detection limit and is suitable for Alternaria genetic detection with its high sensitivity.
Article
Chemistry, Inorganic & Nuclear
Yue Liu, Renata Pereira Lopes, Tanja Ludtke, Desire Di Silvio, Sergio Moya, Jean-Rene Hamon, Didier Astruc
Summary: Dendrimers have been successfully used in nanoparticle catalysis, particularly for Pd NP protection towards carbon-carbon coupling reactions. This study found that dendrimer 1-Pd nanoparticles showed the best catalytic activity. Additionally, both dendrimer-Pd nanocatalysts exhibited excellent robustness and detection sensitivity.
INORGANIC CHEMISTRY FRONTIERS
(2021)
Review
Chemistry, Multidisciplinary
Lei Jiao, Weiqing Xu, Yu Wu, Hongye Yan, Wenling Gu, Dan Du, Yuehe Lin, Chengzhou Zhu
Summary: The development of highly sensitive biosensors has been a focus in recent years. Single-atom catalysts show promise in enhancing signal amplification in biosensing, offering improved sensitivity and selectivity through tunable metal-support interactions and the structural features of active sites.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Spectroscopy
Fatemeh Behrouzifar, Seyed-Ahmad Shahidi, Fereshteh Chekin, Shabnam Hosseini, Azade Ghorbani-HasanSaraei
Summary: A simple and sensitive colorimetric assay for hydrogen peroxide detection based on ABTS oxidation by UV-Vis spectroscopy was developed in this study. The HRP-rGO hybrid biosensor, with excellent detection limit and wide linear ranges, showed great potential for monitoring H2O2 in different beverages. The formation of green product with absorption peaks at 421, 655 and 737 nm was catalyzed by the HRP-rGO hybrid, which was confirmed by various characterization techniques.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Ziyi Zhang, Dan Liu, Xiaoshuo Zhang, Xueli Luo, Wanmei Lin, Zhonghong Li, Jihong Huang
Summary: Novel method using Ag nanoparticles deposited on carbon microspheres was developed for high-performance detection of Hg2+ in seafood. The Ag NPs-CMs nanocomplex showed excellent peroxidase-like activity and could specifically enhance catalytic activity in the presence of Hg2+. The method exhibited a low detection limit and satisfactory recoveries for Hg2+ detection in seafood, and a portable hydrogel kit was designed for on-site detection. This work provides a reliable method for visual Hg2+ detection in seafood and a feasible strategy for the design of high-performance nanozymes.
Article
Chemistry, Analytical
Ruixiang Liu, Zhongyu Cai, Qingsong Zhang, Heng Yuan, Guanglei Zhang, De'an Yang
Summary: A novel sensor for monitoring the concentration of H2O2 has been developed using a label-free two-dimensional photonic crystal (PhC) and protein composite hydrogel. The sensor demonstrates good selectivity and sensitivity, and has the potential to accurately detect low concentrations of H2O2 without the need for complex instruments or high costs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Engineering, Environmental
Yu Mao, Shengjie Gao, Lili Yao, Lu Wang, Hao Qu, Yuen Wu, Ying Chen, Lei Zheng
Summary: The article introduces a colorimetric sensing method for Cr(VI) based on single-atom nanozymes, achieving detection through specific interactions, with high sensitivity and selectivity, and successful application in Cr(VI) detection in water and food samples.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Sundas Jamil, Muhammad Nasir, Yaqeen Ali, Sohail Nadeem, Sidra Rashid, Muhammad Yaqoob Javed, Akhtar Hayat
Summary: A portable and biomimetic nanosensor modified with Cr2O3-TiO2 was designed for robust and colorimetric sensing of hydrogen peroxide. Utilizing a paper-based colorimetric platform, the sensor showed linear detection of H2O2 with excellent anti-interference potential.
Article
Chemistry, Multidisciplinary
Elissa K. Leonard, Miguel Aller Pellitero, Boris Juelg, Jamie B. Spangler, Netzahualcoyotl Arroyo-Curras
Summary: Rapid and accurate diagnostics are crucial for controlling the COVID-19 pandemic and future infectious disease outbreaks. Researchers have developed a novel enzymatic reporter that can detect disease-specific antibodies by producing glucose when binding to patient antibodies. The fusion protein used in this method has shown precise agreement with commercial immunoassays, making it a versatile tool for detecting disease-specific antibodies.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Justyna Glowacka, Kamil Strzelak, Robert Koncki
Summary: The fully mechanized multicommutated flow analysis (MCFA) system developed is dedicated to determining horseradish peroxidase (HRP) activity, featuring a broad linear response range, low detection limit, high throughput, and acceptable precision. Moreover, it can be utilized for detecting HRP inhibitors and selected thiols at micromolar levels, showcasing its potential practical utility in various applications.
Article
Chemistry, Analytical
Zixin Zhou, Wenting He, Hao Chao, Han Wang, Ping Su, Jiayi Song, Yi Yang
Summary: In this study, a specific hemin-based mimetic enzyme was synthesized by assembling zeolitic imidazolate framework-L (ZIF-L) and hemin. The obtained mimetic enzyme displayed enhanced peroxidase activity and resistance to severe conditions. It also served as a colorimetric sensor for uranium detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Li Wu, Shihan Xu, Jingang Wang, Amy L. Paguirigan, Jerald P. Radich, Yuling Qin, Daniel T. Chiu
Summary: Researchers have developed a single cell dispenser inspired by electrohydrodynamic jet printing, which can be used for single-cell multiomics studies, achieving accurate droplet generation and single-cell dispensing through systematic parameter optimization.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiaofang Chen, Min Feng, Xiaoyi Xie, Yi Zhang, Jie Zhang, Xiupei Yang
Summary: Single-atom catalysts based on single Fe atoms on fluorine-doped ultrathin carbon nanosheets were synthesized and demonstrated superior catalytic activity and stability. A sensitive and selective colorimetric detection method for p-phenylenediamine in hair dyes and products after dyeing was developed using this catalyst.