Cisgenesis and intragenesis in microalgae: promising advancements towards sustainable metabolites production

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

(2016) Thanyanan Wannathong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Progress toward isolation of strains and genetically engineered strains of microalgae for production of biofuel and other value added chemicals: A review

(2016) Ashmita Ghosh et al.   ENERGY CONVERSION AND MANAGEMENT

Statistical framework for detection of genetically modified organisms based on Next Generation Sequencing

(2016) Sander Willems et al.   FOOD CHEMISTRY

A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica

(2016) Da-Wei Li et al.   JOURNAL OF BIOTECHNOLOGY

Cisgenesis and genome editing: combining concepts and efforts for a smarter use of genetic resources in crop breeding

(2016) Teodoro Cardi   PLANT BREEDING

Opportunities for Products of New Plant Breeding Techniques

(2016) Jan G. Schaart et al.   TRENDS IN PLANT SCIENCE

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Durable Late Blight Resistance in Potato Through Dynamic Varieties Obtained by Cisgenesis: Scientific and Societal Advances in the DuRPh Project

(2016) A. J. Haverkort et al.   POTATO RESEARCH

Genetic Engineering: A Promising Tool to Engender Physiological, Biochemical, and Molecular Stress Resilience in Green Microalgae

(2016) Freddy Guihéneuf et al.   Frontiers in Plant Science

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Genetic tools and techniques for Chlamydomonas reinhardtii

(2015) Jan H. Mussgnug   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improving microalgae for biotechnology — From genetics to synthetic biology

(2015) Monika Hlavova et al.   BIOTECHNOLOGY ADVANCES

Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

(2015) Yuki Kasai et al.   Biotechnology for Biofuels

Regulation of astaxanthin and its intermediates through cloning and genetic transformation of β-carotene ketolase in Haematococcus pluvialis

(2015) S. Kathiresan et al.   JOURNAL OF BIOTECHNOLOGY

Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

(2015) Antonia A. Dominguez et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Are all GMOs the same? Consumer acceptance of cisgenic rice in India

(2015) Aaron M. Shew et al.   PLANT BIOTECHNOLOGY JOURNAL

Molecular techniques to interrogate and edit theChlamydomonasnuclear genome

(2015) Robert E. Jinkerson et al.   PLANT JOURNAL

Chlamydomonas as a model for biofuels and bio-products production

(2015) Melissa A. Scranton et al.   PLANT JOURNAL

Non-GMO genetically edited crop plants

(2015) Chidananda Nagamangala Kanchiswamy et al.   TRENDS IN BIOTECHNOLOGY

Towards social acceptance of plant breeding by genome editing

(2015) Motoko Araki et al.   TRENDS IN PLANT SCIENCE

Comparative genomics reveals diversified CRISPR-Cas systems of globally distributed Microcystis aeruginosa, a freshwater bloom-forming cyanobacterium

(2015) Chen Yang et al.   Frontiers in Microbiology

In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity

(2015) Javier A. Gimpel et al.   Frontiers in Microbiology

Genetic engineering of the green alga Chlorella zofingiensis: a modified norflurazon-resistant phytoene desaturase gene as a dominant selectable marker

(2014) Jin Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cisgenesis and Intragenesis: New tools For Improving Crops

(2014) C Espinoza et al.   BIOLOGICAL RESEARCH

Production of recombinant proteins in microalgae at pilot greenhouse scale

(2014) Javier A. Gimpel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Delta 5 Fatty Acid Desaturase Upregulates the Synthesis of Polyunsaturated Fatty Acids in the Marine Diatom Phaeodactylum tricornutum

(2014) Kun-Tao Peng et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Genetically engineered crops that fly under the US regulatory radar

(2014) Alex Camacho et al.   NATURE BIOTECHNOLOGY

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis

(2014) Philip D. Weyman et al.   PLANT BIOTECHNOLOGY JOURNAL

From dead leaf, to new life: TAL effectors as tools for synthetic biology

(2014) Orlando de Lange et al.   PLANT JOURNAL

Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker

(2014) Yulin Cui et al.   PLoS One

Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas

(2014) KaiJing Zheng et al.   Science China-Life Sciences

Caution required for handling genome editing technology

(2014) Motoko Araki et al.   TRENDS IN BIOTECHNOLOGY

The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins

(2014) Fariba Akbari et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Precision Genome Engineering and Agriculture: Opportunities and Regulatory Challenges

(2014) Daniel F. Voytas et al.   PLOS BIOLOGY

TALE activation of endogenous genes in Chlamydomonas reinhardtii

(2014) Han Gao et al.   Algal Research-Biomass Biofuels and Bioproducts

Algae-based oral recombinant vaccines

(2014) Elizabeth A. Specht et al.   Frontiers in Microbiology

Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

(2014) Alma Lorena Almaraz-Delgado et al.   AMB Express

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

(2013) James A. Gregory et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of anti-cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners

(2013) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

Biofuels for the 21st century

(2013) Michael D Burkart et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Advances in microalgae engineering and synthetic biology applications for biofuel production

(2013) Javier A Gimpel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Expression of a type 2 diacylglycerol acyltransferase fromThalassiosira pseudonanain yeast leads to incorporation of docosahexaenoic acid β-oxidation intermediates into triacylglycerol

(2013) Jingyu Xu et al.   FEBS Journal

High-value products from microalgae—their development and commercialisation

(2013) Michael A. Borowitzka   JOURNAL OF APPLIED PHYCOLOGY

Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum

(2013) Ying-Fang Niu et al.   Marine Drugs

Identification and Functional Characterization of Genes Encoding Omega-3 Polyunsaturated Fatty Acid Biosynthetic Activities from Unicellular Microalgae

(2013) Royah Vaezi et al.   Marine Drugs

Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids

(2013) Mary L. Hamilton et al.   METABOLIC ENGINEERING

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Intragenesis and cisgenesis as alternatives to transgenic crop development

(2013) Inger Baeksted Holme et al.   PLANT BIOTECHNOLOGY JOURNAL

Synthetic nucleases for genome engineering in plants: prospects for a bright future

(2013) Holger Puchta et al.   PLANT JOURNAL

Precise plant breeding using new genome editing techniques: opportunities, safety and regulation in the EU

(2013) Frank Hartung et al.   PLANT JOURNAL

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection

(2013) Olivia C. Demurtas et al.   PLoS One

Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids

(2013) Jin Liu et al.   PROCESS BIOCHEMISTRY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead

(2013) Yann Devos et al.   TRANSGENIC RESEARCH

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Carotenoid and lipid production by the autotrophic microalga Chlorella protothecoides under nutritional, salinity, and luminosity stress conditions

(2012) L. Campenni’ et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii

(2012) Carla S. Jones et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Advances in genetic engineering of marine algae

(2012) Song Qin et al.   BIOTECHNOLOGY ADVANCES

Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern

(2012) Narendra Tuteja et al.   JOURNAL OF BIOSCIENCES

CHLOROPLAST GENETIC TOOL FOR THE GREEN MICROALGAE HAEMATOCOCCUS PLUVIALIS (CHLOROPHYCEAE, VOLVOCALES)1

(2012) Carla L. Gutiérrez et al.   JOURNAL OF PHYCOLOGY

Isolation and Characterization of a Lycopene ε-Cyclase Gene of Chlorella (Chromochloris) zofingiensis. Regulation of the Carotenogenic Pathway by Nitrogen and Light

(2012) Baldo F. Cordero et al.   Marine Drugs

Microalgal biofactories: a promising approach towards sustainable omega-3 fatty acid production

(2012) T Adarme-Vega et al.   Microbial Cell Factories

FLASH assembly of TALENs for high-throughput genome editing

(2012) Deepak Reyon et al.   NATURE BIOTECHNOLOGY

Deployment of new biotechnologies in plant breeding

(2012) Maria Lusser et al.   NATURE BIOTECHNOLOGY

Nuclear gene targeting inChlamydomonasusing engineered zinc-finger nucleases

(2012) Irina Sizova et al.   PLANT JOURNAL

Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes

(2012) Bulukani Mlalazi et al.   PLANTA

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

(2012) M. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Initial risk assessment of genetically modified (GM) microalgae for commodity-scale biofuel cultivation

(2012) William J. Henley et al.   Algal Research-Biomass Biofuels and Bioproducts

Steps toward a globally available malaria vaccine

(2012) Carla S. Jones et al.   Bioengineered

Toward two decades of plant biotechnology: successes, failures, and prospects

(2012) Nigel G. Halford   Food and Energy Security

Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis

(2011) Baldo F. Cordero et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Screening of marine microalgae for biodiesel feedstock

(2011) Thi Thai Yen Doan et al.   BIOMASS & BIOENERGY

The development of a cisgenic apple plant

(2011) Thalia Vanblaere et al.   JOURNAL OF BIOTECHNOLOGY

Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis

(2011) Y.-J. Zhong et al.   JOURNAL OF EXPERIMENTAL BOTANY

Marine Carotenoids: Biological Functions and Commercial Applications

(2011) Carlos Vílchez et al.   Marine Drugs

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Cisgenic barley with improved phytase activity

(2011) Inger B. Holme et al.   PLANT BIOTECHNOLOGY JOURNAL

TAL Effectors: Customizable Proteins for DNA Targeting

(2011) A. J. Bogdanove et al.   SCIENCE

Fruit improvement using intragenesis and artificial microRNA

(2011) Barbara Molesini et al.   TRENDS IN BIOTECHNOLOGY

Perspectives on metabolic engineering for increased lipid contents in microalgae

(2011) Holger Schuhmann et al.   Biofuels-UK

XanthomonasAvrBs3 Family-Type III Effectors: Discovery and Function

(2010) Jens Boch et al.   Annual Review of Phytopathology

Die vielfältige Anwendung von Mikroalgen als nachwachsende Rohstoffe

(2010) R. Rosello Sastre et al.   CHEMIE INGENIEUR TECHNIK

TAL effectors: finding plant genes for disease and defense

(2010) Adam J Bogdanove et al.   CURRENT OPINION IN PLANT BIOLOGY

Genetic Engineering of Algae for Enhanced Biofuel Production

(2010) Randor Radakovits et al.   EUKARYOTIC CELL

Analysis of an Essential Carotenogenic Enzyme: ζ-Carotene Desaturase from Unicellular AlgaDunaliella salina

(2010) Zhi-Wei Ye et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISOLATION AND CHARACTERIZATION OF A LYCOPENE β-CYCLASE GENE FROM THE ASTAXANTHIN-PRODUCING GREEN ALGA CHLORELLA ZOFINGIENSIS (CHLOROPHYTA)1

(2010) Baldo F. Cordero et al.   JOURNAL OF PHYCOLOGY

CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea

(2010) Luciano A. Marraffini et al.   NATURE REVIEWS GENETICS

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

(2010) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) gene from the microalga O. tauri

(2010) Martin Wagner et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Commercial application of microalgae other than as biofuels: a brief review

(2010) John J. Milledge   REVIEWS IN ENVIRONMENTAL SCIENCE AND BIO-TECHNOLOGY

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Biofuels from algae: challenges and potential

(2010) Michael Hannon et al.   Biofuels-UK

TheARG9Gene Encodes the Plastid-ResidentN-Acetyl Ornithine Aminotransferase in the Green AlgaChlamydomonas reinhardtii

(2009) Claire Remacle et al.   EUKARYOTIC CELL

Isolation and Characterisation of a High-Efficiency Desaturase and Elongases from Microalgae for Transgenic LC-PUFA Production

(2009) James R. Petrie et al.   MARINE BIOTECHNOLOGY

Phylogenetic relationships and species circumscription in Trentepohlia and Printzina (Trentepohliales, Chlorophyta)

(2009) Fabio Rindi et al.   MOLECULAR PHYLOGENETICS AND EVOLUTION

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens

(2009) P. Romer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products

(2009) Liam Brennan et al.   RENEWABLE & SUSTAINABLE ENERGY REVIEWS

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii

(2008) Xiaoyuan Chi et al.   JOURNAL OF MICROBIOLOGY

ISOLATION AND CHARACTERIZATION OF THE PHYTOENE DESATURASE GENE AS A POTENTIAL SELECTIVE MARKER FOR GENETIC ENGINEERING OF THE ASTAXANTHIN-PRODUCING GREEN ALGACHLORELLA ZOFINGIENSIS(CHLOROPHYTA)

(2008) Junchao Huang et al.   JOURNAL OF PHYCOLOGY

Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark-grown Chlorella zofingiensis (Chlorophyceae)

(2008) Yantao Li et al.   PLANTA

Cisgenesis and intragenesis, sisters in innovative plant breeding

(2008) Henk J. Schouten et al.   TRENDS IN PLANT SCIENCE