From dead leaf, to new life: TAL effectors as tools for synthetic biology

BurrH: a new modular DNA binding protein for genome engineering

(2014) Alexandre Juillerat et al.   Scientific Reports

Plant Genome Engineering with Sequence-Specific Nucleases

(2013) Daniel F. Voytas   Annual Review of Plant Biology

Creating a TALE protein with unbiased 5′-T binding

(2013) Shogo Tsuji et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

New and TALENted Genome Engineering Toolbox

(2013) Jarryd M. Campbell et al.   CIRCULATION RESEARCH

Activities and specificities of homodimeric TALENs in Saccharomyces cerevisiae

(2013) Mustapha Aouida et al.   CURRENT GENETICS

Designer TAL Effectors Induce Disease Susceptibility and Resistance to Xanthomonas oryzae pv. Oryzae in Rice

(2013) Ting Li et al.   Molecular Plant

Genome-scale engineering for systems and synthetic biology

(2013) K. M. Esvelt et al.   Molecular Systems Biology

An Efficient Antiviral Strategy for Targeting Hepatitis B Virus Genome Using Transcription Activator-Like Effector Nucleases

(2013) Jieliang Chen et al.   MOLECULAR THERAPY

TET enzymes, TDG and the dynamics of DNA demethylation

(2013) Rahul M. Kohli et al.   NATURE

Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins

(2013) Morgan L Maeder et al.   NATURE BIOTECHNOLOGY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Locus-specific editing of histone modifications at endogenous enhancers

(2013) Eric M Mendenhall et al.   NATURE BIOTECHNOLOGY

A library of TAL effector nucleases spanning the human genome

(2013) Yongsub Kim et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Robust, synergistic regulation of human gene expression using TALE activators

(2013) Morgan L Maeder et al.   NATURE METHODS

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Synergistic and tunable human gene activation by combinations of synthetic transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

Live visualization of chromatin dynamics with fluorescent TALEs

(2013) Yusuke Miyanari et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Breaking the DNA-binding code ofRalstonia solanacearumTAL effectors provides new possibilities to generate plant resistance genes against bacterial wilt disease

(2013) Orlando de Lange et al.   NEW PHYTOLOGIST

Five phylogenetically close riceSWEETgenes confer TAL effector-mediated susceptibility toXanthomonas oryzaepv.oryzae

(2013) Jana Streubel et al.   NEW PHYTOLOGIST

Quantitative analysis of TALE–DNA interactions suggests polarity effects

(2013) Joshua F. Meckler et al.   NUCLEIC ACIDS RESEARCH

Transcription activator like effector (TALE)-directed piggyBac transposition in human cells

(2013) Jesse B. Owens et al.   NUCLEIC ACIDS RESEARCH

Targeting and tracing of specific DNA sequences with dTALEs in living cells

(2013) Katharina Thanisch et al.   NUCLEIC ACIDS RESEARCH

Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats

(2013) Lilia Gabsalilow et al.   NUCLEIC ACIDS RESEARCH

Two- and three-input TALE-based AND logic computation in embryonic stem cells

(2013) Florian Lienert et al.   NUCLEIC ACIDS RESEARCH

Directed evolution of the TALE N-terminal domain for recognition of all 5′ bases

(2013) Brian M. Lamb et al.   NUCLEIC ACIDS RESEARCH

Synthetic nucleases for genome engineering in plants: prospects for a bright future

(2013) Holger Puchta et al.   PLANT JOURNAL

An Improved Method for TAL Effectors DNA-Binding Sites Prediction Reveals Functional Convergence in TAL Repertoires of Xanthomonas oryzae Strains

(2013) Alvaro L. Pérez-Quintero et al.   PLoS One

TAL Effectors Specificity Stems from Negative Discrimination

(2013) Basile I. M. Wicky et al.   PLoS One

TALE-PvuII Fusion Proteins – Novel Tools for Gene Targeting

(2013) Mert Yanik et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA-targeting proteins

(2013) Erin L. Doyle et al.   TRENDS IN CELL BIOLOGY

Compact designer TALENs for efficient genome engineering

(2013) Marine Beurdeley et al.   Nature Communications

RNA-Guided Genome Editing for Target Gene Mutations in Wheat

(2013) Santosh Kumar Upadhyay et al.   G3-Genes Genomes Genetics

Computational Predictions Provide Insights into the Biology of TAL Effector Target Sites

(2013) Jan Grau et al.   PLoS Computational Biology

Predicting promoters targeted by TAL effectors in plant genomes: from dream to reality

(2013) Laurent D. Noël et al.   Frontiers in Plant Science

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the novel modular DNA-binding protein BurrH in its apo form and in complex with its target DNA

(2013) Stefano Stella et al.   Acta Crystallographica Section F-Structural Biology Communications

Crystal structure of a TALE protein reveals an extended N-terminal DNA binding region

(2012) Haishan Gao et al.   CELL RESEARCH

Recognition of methylated DNA by TAL effectors

(2012) Dong Deng et al.   CELL RESEARCH

TAL effectors: function, structure, engineering and applications

(2012) Amanda Nga-Sze Mak et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Overcoming Transcription Activator-like Effector (TALE) DNA Binding Domain Sensitivity to Cytosine Methylation

(2012) Julien Valton et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

FLASH assembly of TALENs for high-throughput genome editing

(2012) Deepak Reyon et al.   NATURE BIOTECHNOLOGY

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

TAL effector RVD specificities and efficiencies

(2012) Jana Streubel et al.   NATURE BIOTECHNOLOGY

A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes

(2012) Jonathan L Schmid-Burgk et al.   NATURE BIOTECHNOLOGY

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Chimeric TALE recombinases with programmable DNA sequence specificity

(2012) Andrew C. Mercer et al.   NUCLEIC ACIDS RESEARCH

Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells

(2012) Maarten Holkers et al.   NUCLEIC ACIDS RESEARCH

Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers

(2012) Sebastian Bultmann et al.   NUCLEIC ACIDS RESEARCH

Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers

(2012) Adrian W. Briggs et al.   NUCLEIC ACIDS RESEARCH

A tunable zinc finger-based framework for Boolean logic computation in mammalian cells

(2012) Jason J. Lohmueller et al.   NUCLEIC ACIDS RESEARCH

Engineering synthetic TAL effectors with orthogonal target sites

(2012) Abhishek Garg et al.   NUCLEIC ACIDS RESEARCH

Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology

(2012) Benjamin A. Blount et al.   PLoS One

Targeting G with TAL Effectors: A Comparison of Activities of TALENs Constructed with NN and NK Repeat Variable Di-Residues

(2012) Michelle L. Christian et al.   PLoS One

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

(2012) A. N.-S. Mak et al.   SCIENCE

Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors

(2012) D. Deng et al.   SCIENCE

Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains

(2012) Le Cong et al.   Nature Communications

Transcription activator-like effector hybrids for conditional control and rewiring of chromosomal transgene expression

(2012) Yi Li et al.   Scientific Reports

Synthetic Biology and Metabolic Engineering

(2012) Gregory Stephanopoulos   ACS Synthetic Biology

Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription

(2011) Feng Zhang et al.   NATURE BIOTECHNOLOGY

Transcription by RNA polymerase III: more complex than we thought

(2011) Robert J. White   NATURE REVIEWS GENETICS

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Assembly of custom TALE-type DNA binding domains by modular cloning

(2011) Robert Morbitzer et al.   NUCLEIC ACIDS RESEARCH

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

(2011) Ting Li et al.   NUCLEIC ACIDS RESEARCH

A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity

(2011) Claudio Mussolino et al.   NUCLEIC ACIDS RESEARCH

Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein

(2011) Magdy M. Mahfouz et al.   PLANT MOLECULAR BIOLOGY

Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway

(2011) Mauricio S. Antunes et al.   PLoS One

Transcriptional Activators of Human Genes with Programmable DNA-Specificity

(2011) René Geiβler et al.   PLoS One

TAL Effectors: Customizable Proteins for DNA Targeting

(2011) A. J. Bogdanove et al.   SCIENCE

XanthomonasAvrBs3 Family-Type III Effectors: Discovery and Function

(2010) Jens Boch et al.   Annual Review of Phytopathology

DNA Transposons: Nature and Applications in Genomics

(2010) Martin Munoz-Lopez et al.   CURRENT GENOMICS

Complete Genome Sequence of Burkholderia rhizoxinica, an Endosymbiont of Rhizopus microsporus

(2010) G. Lackner et al.   JOURNAL OF BACTERIOLOGY

A comparative analysis of synthetic genetic oscillators

(2010) O. Purcell et al.   Journal of the Royal Society Interface

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors

(2010) R. Morbitzer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recognition of AvrBs3-Like Proteins Is Mediated by Specific Binding to Promoters of Matching Pepper Bs3 Alleles

(2009) P. Romer et al.   PLANT PHYSIOLOGY

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Purple as a tomato: towards high anthocyanin tomatoes

(2009) Silvia Gonzali et al.   TRENDS IN PLANT SCIENCE

Knowledge-making distinctions in synthetic biology

(2007) Maureen A. O'Malley et al.   BIOESSAYS