Article
Biochemistry & Molecular Biology
Natalie Laspata, Parminder Kaur, Sofiane Yacine Mersaoui, Daniela Muoio, Zhiyan Silvia Liu, Maxwell Henry Bannister, Hai Dang Nguyen, Caroline Curry, John M. Pascal, Guy G. Poirier, Hong Wang, Jean-Yves Masson, Elise Fouquerel
Summary: PARP1 is a DNA-dependent ADP-Ribose transferase that is involved in resolving DNA breaks and non-B DNA structures. It has been identified as a component of the R-loop-associated protein-protein interaction network, suggesting its potential role in resolving R-loop structures. R-loops are three-stranded nucleic acid structures that can cause genome instability if persistently unresolved.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Morgan Dasovich, Morgan Q. Beckett, Scott Bailey, Shao-En Ong, Marc M. Greenberg, Anthony K. L. Leung
Summary: The study synthesized a PAR photoaffinity probe to identify numerous PAR binding proteins involved in DNA repair, RNA processing, and metabolism, demonstrating that polymer length may regulate the outcome and timing of PAR signaling pathways. This investigation provides the first census of PAR-binding proteins, offers a proteomics analysis of length-selective PAR binding, and associates PAR binding with RNA metabolism and the formation of biomolecular condensates.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Johannes Rudolph, Genevieve Roberts, Karolin Luger
Summary: PARP1 and PARP2 are key enzymes in the DNA damage response. HPF1 plays an essential role in modulating the PARylation of histones by forming a complex with both PARP1 and PARP2. Results show how HPF1 can affect the binding affinity of certain inhibitors to PARP1.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Huanyi Fu, Rongdiao Liu, Zixuan Jia, Ran Li, Feifeng Zhu, Wenxuan Zhu, Yangqing Shao, Yiyang Jin, Yuhua Xue, Jun Huang, Kunxin Luo, Xiang Gao, Huasong Lu, Qiang Zhou
Summary: DNA damage leads to transcriptional stalling to prevent mutagenesis and initiate repair signaling. Poly(ADP-ribose) polymerase (PARP1) inhibits Pol II elongation by inactivating the transcription elongation factor P-TEFb, promoting DNA repair and cell survival.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Evgeniia Prokhorova, Florian Zobel, Rebecca Smith, Siham Zentout, Ian Gibbs-Seymour, Kira Schutzenhofer, Alessandra Peters, Josephine Groslambert, Valentina Zorzini, Thomas Agnew, John Brognard, Michael L. Nielsen, Dragana Ahel, Sebastien Huet, Marcin J. Suskiewicz, Ivan Ahel
Summary: Recent findings suggest that serine ADP-ribosylation plays a vital role in cellular responses to PARP1/PARP2 inhibitors. The efficient modification of three serine residues within PARP1 by HPF1 counters PARP1 trapping and contributes to inhibitor tolerance, implicating these residues as potential biomarkers for PARP inhibitor therapy.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Johannes Rudolph, Genevieve Roberts, Uma M. Muthurajan, Karolin Luger
Summary: HPF1 redirects PARylation by PARP1 towards histones, leading to shorter PAR chains and production of free ADP-ribose. This switch from polymerase to hydrolase has important implications for DNA damage response mediated by PARP1 and raises questions about intracellular ADPR and depletion of NAD(+).
Article
Biology
Tatyana A. Kurgina, Nina A. Moor, Mikhail M. Kutuzov, Konstantin N. Naumenko, Alexander A. Ukraintsev, Olga Lavrik
Summary: The histone PARylation factor 1 (HPF1) has been found to stimulate the autoPARylation of PARP1 and PARP2 as well as the heteroPARylation of histones in the complex with nucleosome. The stimulatory action is dependent on the concentrations of HPF1 and NAD(+), with PARP2 showing more efficiency than PARP1 in the reaction. The dual function of HPF1 in maintaining PARP activity is also discussed.
COMMUNICATIONS BIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Yan Wang, David Pleasure, Wenbin Deng, Fuzheng Guo
Summary: PARP1 plays a critical role in DNA repair and gene expression, and its dysregulation is associated with immune activation and disease severity. Studies have shown that PARP1 dysfunction is present in the immune and central nervous system of MS patients and animal models, and its function is complex and context-dependent.
Article
Multidisciplinary Sciences
Fen Yang, Jianji Chen, Bin Liu, Guozhen Gao, Manu Sebastian, Collene Jeter, Jianjun Shen, Maria D. Person, Mark T. Bedford
Summary: SPINDOC interacts with histone code effector protein SPIN1 and forms two distinct protein complexes, one with SPIN1 and the other with PARP1. It plays a role in regulating PARP1-mediated PARylation and the DNA damage response. Knockout of SPINDOC leads to reduced PARylation levels, smaller body size in mice, and hypersensitivity to IR-induced DNA damage.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Albert T. Lam, Xiao-Nan Zhang, Valentine V. Courouble, Timothy S. Strutzenberg, Hua Pei, Bangyan L. Stiles, Stan G. Louie, Patrick R. Griffin, Yong Zhang
Summary: Protein poly-ADP-ribosylation is a heterogeneous and dynamic post-translational modification regulated by various factors, participating in biological events and human diseases. Currently, tools are lacking for clearly defining readers and erasers of PARylated proteins. The newly developed bifunctional NAD(+) serves as an important tool for studying cellular interaction networks centered on protein PARylation.
ACS CHEMICAL BIOLOGY
(2021)
Article
Cell Biology
Wei Chen, E. Qiukai, Bo Sun, Pengxue Zhang, Nan Li, Shujia Fei, Yingnan Wang, Shuting Liu, Xiaoqiu Liu, Xuesen Zhang
Summary: By studying a mouse follicle reconstitution model, researchers found that PARP1 enzymatic activity in granulosa cells determines the activation of dormant primordial follicles. Inhibiting PARP1 can prevent the depletion of the primordial follicle pool caused by excessive endoplasmic reticulum stress. This study demonstrates the important regulatory role of PARP1 in granulosa cells and suggests it as a potential therapeutic target for maintaining the primordial follicle pool in females.
CELL DEATH & DISEASE
(2023)
Article
Chemistry, Multidisciplinary
Xiao-Nan Zhang, Albert T. Lam, Qinqin Cheng, Valentine V. Courouble, Timothy S. Strutzenberg, Jiawei Li, Yiling Wang, Hua Pei, Bangyan L. Stiles, Stan G. Louie, Patrick R. Griffin, Yong Zhang
Summary: Researchers have reported a synthetic NAD(+) compound featuring high activity and specificity for human PARP1, which can be used as an ADP-ribose donor to label and analyze potential protein substrates of endogenous PARP1.
Article
Cell Biology
Marek Adamowicz, Richard Hailstone, Annie A. Demin, Emilia Komulainen, Hana Hanzlikova, Jan Brazina, Amit Gautam, Sophie E. Wells, Keith W. Caldecott
Summary: Toxic PARP1 activity induced by mutations in XRCC1 impairs global transcription recovery during DNA base excision repair by promoting aberrant recruitment and activity of the ubiquitin protease USP3. Inhibition or deletion of PARP1 or USP3 restores transcriptional recovery in XRCC1-deficient cells, suggesting them as potential therapeutic targets in neurological disease.
NATURE CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anka Gueldenpfennig, Ann-Katrin Hopp, Lukas Muskalla, Patrick Manetsch, Fabio Raith, Lars Hellweg, Cyril Doerdelmann, Deena M. Leslie Pedrioli, Kai Johnsson, Giulio Superti-Furga, Michael O. Hottiger
Summary: Although the effect of SLC25A51 on glucose metabolism is known, its role in other NAD(+)-dependent processes such as ADP-ribosylation is unclear. This study shows that absence of SLC25A51 leads to increased NAD(+) concentration in both the cytoplasm and nucleus, resulting in enhanced PARP1-mediated nuclear ADP-ribosylation and accelerated repair of DNA lesions. Furthermore, the absence of SLC25A51 reduces the sensitivity of breast cancer cells to PARP1 inhibition. These findings suggest that SLC25A51 has potential as a target for improving PARP1 inhibitor-based therapies.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Perumal Arumugam Desingu, Sneha Mishra, Lavanya Dindi, Shalini Srinivasan, Raju S. Rajmani, Venkatraman Ravi, Ankit Kumar Tamta, Sukanya Raghu, Krishnega Murugasamy, Anwit Shriniwas Pandit, Nagalingam R. Sundaresan
Summary: This study demonstrates that inhibition of PARP1 can protect against Japanese encephalitis virus (JEV) infection by inhibiting autophagy.
Article
Oncology
Huifang Shi, Juechao Zhang, Xiaoqing Han, Huihan Li, Mingshu Xie, Yingying Sun, Wenguang Liu, Xueqing Ba, Xianlu Zeng
INTERNATIONAL JOURNAL OF CANCER
(2017)
Article
Cell Biology
Peter German, David Saenz, Peter Szaniszlo, Leopoldo Aguilera-Aguirre, Lang Pan, Muralidhar L. Hegde, Attila Bacsi, Gyorgy Hajas, Zsolt Radak, Xueqing Ba, Sankar Mitra, John Papaconstantinou, Istvan Boldogh
MECHANISMS OF AGEING AND DEVELOPMENT
(2017)
Article
Multidisciplinary Sciences
Yueshuang Ke, Yanlong Han, Xiaolan Guo, Jitao Wen, Ke Wang, Xue Jiang, Xue Tian, Xueqing Ba, Istvan Boldogh, Xianlu Zeng
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Yueshuang Ke, Ke Wang, Hui Xu, Chenxin Wang, Jing Zhang, Xianlu Zeng, Ruoxi Wang, Istvan Boldogh, Xueqing Ba
MOLECULAR AND CELLULAR PROBES
(2018)
Article
Cell Biology
Ruoxi Wang, Chunshuang Li, Ping Qiao, Yaoyao Xue, Xu Zheng, Hongyu Chen, Xianlu Zeng, Wenguang Liu, Istvan Boldogh, Xueqing Ba
CELL DEATH & DISEASE
(2018)
Article
Cell Biology
Liangping Su, Hongyuan Li, Cheng Huang, Tingting Zhao, Yongjun Zhang, Xueqing Ba, Zhongwei Li, Yu Zhang, Baiqu Huang, Jun Lu, Yanmei Zhao, Xiaoxue Li
Article
Biochemistry & Molecular Biology
Wenjing Hao, Tianyang Qi, Lang Pan, Ruoxi Wang, Bing Zhu, Leopoldo Aguilera-Aguirre, Zsolt Radak, Tapas K. Hazra, Spiros A. Vlahopoulos, Attila Bacsi, Allan R. Brasier, Xueqing Ba, Istvan Boldogh
Review
Biochemistry & Molecular Biology
Xueqing Ba, Istvan Boldogh
Article
Oncology
Huifang Shi, Xiaoqing Han, Yingying Sun, Chao Shang, Min Wei, Xueqing Ba, Xianlu Zeng
Review
Biochemistry & Molecular Biology
Ruoxi Wang, Wenjing Hao, Lang Pan, Istvan Boldogh, Xueqing Ba
CELLULAR AND MOLECULAR LIFE SCIENCES
(2018)
Article
Multidisciplinary Sciences
Torkild Visnes, Armando Cazares-Korner, Wenjing Hao, Olov Wallner, Geoffrey Masuyer, Olga Loseva, Oliver Mortusewicz, Elisee Wiita, Antonio Sarno, Aleksandr Manoilov, Juan Astorga-Wells, Ann-Sofie Jemth, Lang Pan, Kumar Sanjiv, Stella Karsten, Camilla Gokturk, Maurice Grube, Evert J. Homan, Bishoy M. F. Hanna, Cynthia B. J. Paulin, Therese Pham, Azita Rasti, Ulrika Warpman Berglund, Catharina von Nicolai, Carlos Benitez-Buelga, Tobias Koolmeister, Dag Ivanic, Petar Iliev, Martin Scobie, Hans E. Krokan, Pawel Baranczewski, Per Artursson, Mikael Altun, Annika Jenmalm Jensen, Christina Kalderen, Xueqing Ba, Roman A. Zubarev, Pal Stenmark, Istvan Boldogh, Thomas Helleday
Article
Biochemistry & Molecular Biology
Yueshuang Ke, Xueping Lv, Xingyue Fu, Jing Zhang, Ameer Ali Bohio, Xianlu Zeng, Wenjing Hao, Ruoxi Wang, Istvan Boldogh, Xueqing Ba
Summary: PARylation is an important post-translational modification that increases the formation of HuR oligomer/multimer, which in turn promotes the disassociation of miRISC and stabilizes pro-inflammatory gene mRNAs.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lang Pan, Wenjing Hao, Yaoyao Xue, Ke Wang, Xu Zheng, Jixian Luo, Xueqing Ba, Yang Xiang, Xiaoqun Qin, Jesper Bergwik, Lloyd Tanner, Arne Egesten, Allan R. Brasier, Istvan Boldogh
Summary: This study reveals that reactive oxygen species (ROS) can induce epithelial cell-state transition and ECM deposition during airway injury. They found that oxidative DNA damage, specifically the formation of 8-oxoG lesions, functions as a pioneer factor in transcriptional reprogramming of airway epithelial cells. These findings provide important insights into the role of oxidative DNA damage in mediating profibrotic processes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Physiology
Leopoldo Aguilera-Aguirre, Wenging Hao, Lang Pan, Xiaoxue Li, Alfredo Saavedra-Molina, Attila Bacsi, Zsolt Radak, Sanjiv Sur, Allan R. Brasier, Xueqing Ba, Istvan Boldogh
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2017)