Article
Chemistry, Analytical
Xiaohua Chen, Qi Chen, Man Chen, Wenjuan Wang, Chuan Sun, Xinru Wang, Peng Ning, Liling Hou, Yan Feng, Xiangming Meng
Summary: The level of apoptosis is closely related to the occurrence of cancer and other diseases. Real-time monitoring of apoptosis in living cells has potential for early disease diagnosis, therapeutic evaluation, and drug screening. A two-photon fluorescent probe Lyso-PCE was developed for pH detection and lysosome-targeting, demonstrating applications in monitoring pH changes, apoptosis, and evaluating apoptotic drugs like bpV(phen).
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Rui Chen, Kangqiang Qiu, Daniel C. Y. Leong, Bidyut Kumar Kundu, Chengying Zhang, Prasenjit Srivastava, Katie E. White, Guodong Li, Guanqun Han, Ziyuan Guo, Christopher G. Elles, Jiajie Diao, Yujie Sun
Summary: This article introduces three new small-molecule probes with good fluorescence quantum yield and aqueous solubility, which exhibit excellent two-photon absorption in the near-infrared region. Lyso-2arm and Mito-3arm show good targeting abilities for lysosomes and mitochondria, respectively. In two-photon microscopy experiments, Mito-3arm outperforms a commercial two-photon dye, delivering enhanced resolution, broader NIR light excitation window, and higher signal-to-noise ratio. Moreover, the two-photon bioimaging of 3D human forebrain organoids confirms the successful deep tissue imaging capabilities of both Lyso-2arm and Mito-3arm. Overall, this work presents a rational design strategy for developing competent two-photon-absorbing probes by varying the number of conjugated arms for bioimaging applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Hyun Seung No, Taemin Kim, Jong-In Hong
Summary: Glutathione is an abundant biological thiol involved in antioxidant defense systems in human cells, and its lack can increase the risk of oxidative stress, leading to cancer progression. Dual sensors based on iridium complexes were developed for the selective detection of GSH, with high selectivity and successful application for determining GSH concentrations in human serum using ECL methods.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Ben Liu, Jing Long, Meng Zhang, Kaiming Cheng, Xin Gao, Yibo Zhou, Yi Li, Zilong Tang, Wei Zhang
Summary: This study successfully achieved the goal of detecting H2S in mitochondria through exploring an iridium complex, which displays high selectivity and sensitivity, low cell toxicity, and has been successfully used for in vitro and in vivo H2S imaging.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Yibiao Jin, Donghao Hu, Jiamiao Yin, Kuangshi Sun, Lijun Chen, Shujuan Liu, Fuyou Li, Qiang Zhao
Summary: In this study, an iridium-based probe decorated with a p-nitrophenyl group was designed and investigated for the detection and imaging of nitroreductase (NTR). The probe showed selective and precise detection of NTR using photoluminescence lifetime imaging (PLIM) within living cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Multidisciplinary
Rongji Zhu, Peiling Dai, Jun Yang, Jie Zhou, Jin Zhang, Kenneth Yin Zhang, Yonghua Li, Shujuan Liu, Kenneth Kam-Wing Lo, Qiang Zhao
Summary: In this study, dual-emissive iridium(III) complexes were developed to exhibit phosphorescence responses to copper(II) ions at a distinct wavelength from oxygen quenching. These complexes demonstrated colorimetric phosphorescence response in aqueous solutions under different copper(II) and oxygen conditions. In cellular imaging, the intensity and lifetime of green phosphorescence can be modulated by variation in oxygen concentration without affecting the response of red phosphorescence towards intracellular copper(II) ions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Inorganic & Nuclear
Carmen Lorenzo-Aparicio, Mar Gomez Gallego, Carmen Ramirez de Arellano, Miguel A. Sierra
Summary: In this study, new types of neutral and cationic phosphorescent complexes derived from 6-phenylpurine nucleosides and nucleotides were prepared and studied. The neutral complexes exhibit orange-red emission upon photoexcitation, while the cationic complexes emit yellow-green light.
DALTON TRANSACTIONS
(2022)
Article
Chemistry, Applied
Chan Hee Ryu, Junseop Lim, Mingi Kim, Ilsup Shin, Jun Yeob Lee, Kang Mun Lee
Summary: Researchers have developed a blue-emitting iridium complex, IrTrz, based on a 1,2,4-triazole ligand, which exhibits stable blue emission, high thermal stability, and excellent electrochemical stability. Multilayer phosphorescent OLEDs using IrTrz as the blue emitter show low turn-on voltages and high external quantum efficiencies, with stable operational lifetimes exceeding 690 h.
Article
Chemistry, Analytical
Fangfang Meng, Jie Niu, Huamiao Zhang, Rui Yang, Qing Lu, Yi Yu, Zhiqiang Liu, Guangle Niu, Xiaoqiang Yu
Summary: The study developed a polarity-sensitive naphthalimide derivative fluorescent probe LD-Lys with high photostability and low cytotoxicity, which can simultaneously image LDs and lysosomes by showing strong and weak fluorescence, respectively. The dual-color imaging of LDs with bright yellow emission and lysosomes with weak red emission was achieved using a confocal laser scanning microscope. By monitoring the location changes, real-time tracking of LDs and lysosomes in cells and visualizing the size, morphology, and distribution of LDs and lysosomes in different tissues were feasible with LD-Lys, making it a promising fluorescent agent for studying the LD-lysosome interplay and metabolism diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Inorganic & Nuclear
Johannes Soellner, Thomas Strassner
Summary: Iridium(III) complexes with cyclometalating ligands have excellent properties for use as phosphorescent emitters in organic light emitting diodes. A series of complexes with 2-phenyl-pyridine and bis(pyrazolyl)borate ligands showed nearly unitary quantum yields and short excited state lifetimes in sky-blue to green emissions.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Inorganic & Nuclear
Guangfu Li, Tianzhi Yang, Kuizhan Shao, Ying Gao, Guogang Shan, Zhongmin Su, Xinlong Wang, Dongxia Zhu
Summary: Although some MCL-active iridium(III) complexes have been reported, the MCL mechanism is still unclear and requires further study. By comparing two new iridium(III) complex isomers, an extraordinary MCL mechanism has been presented for the first time.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiang-Yu Wang, You-Mei Lin, Xiao-Yan Sun, Ya-Qian Wu, Hui Miao, Jun Chu, Tian-Wen Bai, Ying-Long Fu
Summary: In this study, a fluorescent probe BML was synthesized for the detection of nitric oxide (NO) in lysosomes. The BML probe showed fast detection time, low detection limit, and resistance to interference and acidity, making it suitable for NO detection in lysosomes. The probe was successfully used to detect endogenous and exogenous NO in living cells and zebrafish.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Inorganic & Nuclear
Jie Zhou, Jinlu Li, Kenneth Yin Zhang, Shujuan Liu, Qiang Zhao
Summary: Luminescent iridium(III) complexes have been extensively studied as intracellular sensors for photoluminescence microscopy imaging due to their high phosphorescence quantum yields, photostability, good cell membrane permeability, and rich and sensitive photophysical properties. Photoluminescence lifetime imaging microscopy (PLIM) allows for visualizing and mapping the decay of luminescent dyes in the time domain during cellular imaging. This review article focuses on the design and applications of lifetime-responsive phosphorescent iridium(III) complexes as imaging reagents for mapping environmental parameters and sensing analytes of interest in intracellular conditions.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Chemistry, Inorganic & Nuclear
Zejing Chen, Jiayang Jiang, Weili Zhao, Xiaoming Hu, Mingjuan Xie, Feiyang Li, Shujuan Liu, Qiang Zhao
Summary: Fluoride anion is critical for bone and tooth growth, but overdose can lead to chronic diseases. A novel fluoride anion probe Ir-AF has been developed for accurate measurement in aqueous solutions and high signal-to-noise ratio sensing in living cells.
JOURNAL OF ORGANOMETALLIC CHEMISTRY
(2021)
Article
Crystallography
Song Guo, Chaoxiong Guo, Zhao Lu, Linlin Du, Man Gao, Shujuan Liu, Yuanli Liu, Qiang Zhao
Summary: A novel and sensitive fluorine probe based on an ionic iridium(III) complex was designed and synthesized for accurate detection of fluoride ions in organic solvents. The probe interacts with fluoride ions through hydrogen bonding, leading to significant phosphorescence quenching. This study provides insights into the mechanism using nuclear magnetic resonance titration and theoretical calculations.
Correction
Nanoscience & Nanotechnology
Zhou Liu, Lina Xie, Kangqiang Qiu, Xinxing Liao, Thomas W. Rees, Zizhuo Zhao, Liangnian Ji, Hui Chao
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Chemistry, Multidisciplinary
Shi Kuang, Lingli Sun, Xianrui Zhang, Xinxing Liao, Thomas W. Rees, Leli Zeng, Yu Chen, Xiting Zhang, Liangnian Ji, Hui Chao
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Chemistry, Multidisciplinary
Kai Xiong, Chen Qian, Yixian Yuan, Lin Wei, Xinxing Liao, Liting He, Thomas W. Rees, Yu Chen, Jian Wan, Liangnian Ji, Hui Chao
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Engineering, Biomedical
Jinchao Shen, Thomas W. Rees, Zhiguo Zhou, Shiping Yang, Liangnian Ji, Hui Chao
Article
Chemistry, Multidisciplinary
Cheng Ouyang, Yongguang Li, Thomas W. Rees, Xinxing Liao, Jianhua Jia, Yu Chen, Xiting Zhang, Liangnian Ji, Hui Chao
Summary: The organoplatinum(II) complex Pt1 can assemble into nanoaggregates via pi-pi stacking and complementary hydrogen bonds, exhibiting ratiometric dual emission in cells with intact membranes and disrupted membranes. The unique characteristics of Pt1 enable its use for tracking cell apoptosis, necrosis, and inflammation in zebrafish.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Lili Wang, Ruilin Guan, Lina Xie, Xinxing Liao, Kai Xiong, Thomas W. Rees, Yu Chen, Liangnian Ji, Hui Chao
Summary: The Ir1 iridium complex induces immunogenic cell death in non-small cell lung cancer cells, triggering an anti-tumor T-cell response and resulting in long-lasting anti-tumor immunity through the activation of ICD. Ir1 is the first Ir-based complex capable of developing an immunomodulatory response by inducing immunogenic cell death.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Inorganic & Nuclear
Fangmian Wei, Thomas W. Rees, Xinxing Liao, Liangnian Ji, Hui Chao
Summary: Photodynamic therapy is a non-invasive treatment for cancers and non-malignant diseases, but some agents are limited by their oxygen-dependent nature in hypoxic solid tumors. Recent advances in oxygen self-sufficient nanomaterials offer opportunities to overcome this challenge and improve the efficacy of PDT in hypoxia.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Analytical
Weijun Wu, Xinxing Liao, Yu Chen, Liangnian Ji, Hui Chao
Summary: Peroxynitrite and glutathione play essential roles in regulating oxidation and antioxidation in the body, and studying mitochondria ONOO-/GSH redox cycles is crucial for understanding various diseases. The synthesized Ir-diol fluorescent probe exhibits excellent properties, showing high sensitivity and selectivity towards ONOO-/GSH in aqueous solution and living cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Fangmian Wei, Shi Kuang, Thomas W. Rees, Xinxing Liao, Jiangping Liu, Diqing Luo, Jinquan Wang, Xiting Zhang, Liangnian Ji, Hui Chao
Summary: The novel oxygen self-sufficient photosensitizer Ru-g-C3N4, designed to overcome tumor hypoxia-induced resistance in photodynamic therapy, shows enhanced water solubility, visible light absorption, and biocompatibility. Through catalyzing decomposition of H2O2 and H2O to generate O2, Ru-g-C3N4 significantly improves PDT efficacy by producing multiple reactive oxygen species and alleviating tumor hypoxia. This metal complex-grafted g-C3N4 photosensitizer represents a groundbreaking development in oxygen-producing photodynamic therapy agents.
Review
Chemistry, Inorganic & Nuclear
Jinchao Shen, Thomas W. Rees, Liangnian Ji, Hui Chao
Summary: This review explores the fabrication strategies and potential applications of Ru(II) and Ir(III) complex containing nanosystems, highlighting their recent developments in cancer treatment and bioimaging. Challenges and opportunities in this field are also discussed for future research.
COORDINATION CHEMISTRY REVIEWS
(2021)
Review
Biochemistry & Molecular Biology
Thomas W. Rees, Po-Yu Ho, Jeannine Hess
Summary: Antimicrobial resistance (AMR) is a significant global problem, causing over 1 million deaths in 2019 alone. Antimicrobial photodynamic therapy (aPDT) is a promising solution that uses photosensitizers activated by light to selectively target and kill bacteria. Metal complex photosensitizers have emerged as a potential tool in aPDT due to their excellent optoelectronic properties. This review summarizes the progress made in the last five years in the development of small-molecule metal complex photosensitizers for aPDT.
Article
Chemistry, Inorganic & Nuclear
Jinchao Shen, Xinxing Liao, Weijun Wu, Tao Feng, Johannes Karges, Mingwei Lin, Hongjie Luo, Yu Chen, Hui Chao
Summary: Intracellular calcium levels are closely related to cell survival. This study proposes a novel nano-platform for calcium overload and two-photon photodynamic therapy, which releases Ir(III) complexes and excess calcium ions, resulting in a high cytotoxic effect. The loaded nanoplatform demonstrates a strong tumor growth inhibition effect in breast cancer.
INORGANIC CHEMISTRY FRONTIERS
(2022)
Article
Chemistry, Inorganic & Nuclear
Xinxing Liao, Jinchao Shen, Weijun Wu, Shi Kuang, Mingwei Lin, Johannes Karges, Zilong Tang, Hui Chao
Summary: A mitochondrial-targeting iridium(iii) prodrug responsive to the tumor microenvironment was developed to release photosensitizers for PDT and a glutathione scavenger, leading to increased vulnerability of cancer cells to oxidative stress caused by two-photon photodynamic therapy. This approach showed a strong phototoxic effect towards human gastric adenocarcinoma cellular and animal models upon two-photon laser irradiation (730 nm).
INORGANIC CHEMISTRY FRONTIERS
(2021)
Article
Chemistry, Multidisciplinary
Libing Ke, Fangmian Wei, Xinxing Liao, Thomas W. Rees, Shi Kuang, Zhou Liu, Yu Chen, Liangnian Ji, Hui Chao
Summary: By developing nano-photosensitizers RuS1 NPs and RuS2 NPs, enhanced uptake in cancer cells and improved PDT efficiency were achieved. These nanoparticles can release photosensitizers in high GSH environments while consuming GSH, leading to excellent therapeutic outcomes.
Correction
Chemistry, Inorganic & Nuclear
Ya Wen, Cheng Ouyang, Quanwen Li, Thomas W. Rees, Kangqiang Qiu, Liangnian Ji, Hui Chao
DALTON TRANSACTIONS
(2020)