Terpenoid Metabolic Engineering in Photosynthetic Microorganisms

Biotechnology of cyanobacterial isoprene production

(2018) Julie E. Chaves et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Toolboxes for cyanobacteria: Recent advances and future direction

(2018) Tao Sun et al.   BIOTECHNOLOGY ADVANCES

Developing a Cas9-based tool to engineer native plasmids in Synechocystis sp. PCC 6803

(2018) Yi Xiao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Photomixotrophic chemical production in cyanobacteria

(2018) Morgan M Matson et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae

(2018) Bingyin Peng et al.   METABOLIC ENGINEERING

Integrated analysis of isopentenyl pyrophosphate (IPP) toxicity in isoprenoid-producing Escherichia coli

(2018) Kevin W. George et al.   METABOLIC ENGINEERING

Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound

(2018) Elias Englund et al.   METABOLIC ENGINEERING

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

(2018) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Tailored carbon partitioning for phototrophic production of ( E )-α-bisabolene from the green microalga Chlamydomonas reinhardtii

(2018) Julian Wichmann et al.   METABOLIC ENGINEERING

Enhanced structural diversity in terpenoid biosynthesis: enzymes, substrates and cofactors

(2018) Abith Vattekkatte et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Engineering the unicellular alga Phaeodactylum tricornutum for high-value plant triterpenoid production

(2018) Sarah D'Adamo et al.   PLANT BIOTECHNOLOGY JOURNAL

Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

(2018) Charles M. Denby et al.   Nature Communications

An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum

(2018) Samuel S. Slattery et al.   ACS Synthetic Biology

Bio-solar cell factories for photosynthetic isoprenoids production

(2018) Sung Cheon Ko et al.   PLANTA

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

(2018) Pierre Crozet et al.   ACS Synthetic Biology

Direct Conversion of CO2 to α-Farnesene Using Metabolically Engineered Synechococcus elongatus PCC 7942

(2017) Hyun Jeong Lee et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Relationship between isoprene emission and photosynthesis in diatoms, and its implications for global marine isoprene estimates

(2017) K.G. Srikanta Dani et al.   MARINE CHEMISTRY

Releasing the potential power of terpene synthases by a robust precursor supply platform

(2017) Guangkai Bian et al.   METABOLIC ENGINEERING

A squalene synthase protein degradation method for improved sesquiterpene production in Saccharomyces cerevisiae

(2017) Bingyin Peng et al.   METABOLIC ENGINEERING

Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies

(2017) Emil D. Jensen et al.   Microbial Cell Factories

Responses of Synechocystis sp. PCC 6803 to heterologous biosynthetic pathways

(2017) Konstantinos Vavitsas et al.   Microbial Cell Factories

Photosynthetic fuel for heterologous enzymes: the role of electron carrier proteins

(2017) Silas Busck Mellor et al.   PHOTOSYNTHESIS RESEARCH

Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803

(2017) Po-Cheng Lin et al.   Scientific Reports

Engineering Isoprene Synthase Expression and Activity in Cyanobacteria

(2017) Julie E. Chaves et al.   ACS Synthetic Biology

Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii

(2017) Irini Pateraki et al.   eLife

Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria

(2016) Sun Young Choi et al.   Biotechnology for Biofuels

Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO2

(2016) Xiang Gao et al.   Energy & Environmental Science

Engineering cytosolic acetyl-coenzyme A supply in Saccharomyces cerevisiae : Pathway stoichiometry, free-energy conservation and redox-cofactor balancing

(2016) Harmen M. van Rossum et al.   METABOLIC ENGINEERING

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

(2016) Hung Li et al.   METABOLIC ENGINEERING

Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803

(2016) Artur Wlodarczyk et al.   METABOLIC ENGINEERING

Synergy between methylerythritol phosphate pathway and mevalonate pathway for isoprene production in Escherichia coli

(2016) Chen Yang et al.   METABOLIC ENGINEERING

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

(2016) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Nineteen-step total synthesis of (+)-phorbol

(2016) Shuhei Kawamura et al.   NATURE

Rewriting yeast central carbon metabolism for industrial isoprenoid production

(2016) Adam L. Meadows et al.   NATURE

Why do plants produce so many terpenoid compounds?

(2016) Eran Pichersky et al.   NEW PHYTOLOGIST

Sustainable heterologous production of terpene hydrocarbons in cyanobacteria

(2016) Cinzia Formighieri et al.   PHOTOSYNTHESIS RESEARCH

Extending the biosynthetic repertoires of cyanobacteria and chloroplasts

(2016) Agnieszka Zygadlo Nielsen et al.   PLANT JOURNAL

Synthetic biology for production of natural and new-to-nature terpenoids in photosynthetic organisms

(2016) Philipp Arendt et al.   PLANT JOURNAL

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

(2016) Amanda Hopes et al.   Plant Methods

Genome-Scale Model Reveals Metabolic Basis of Biomass Partitioning in a Model Diatom

(2016) Jennifer Levering et al.   PLoS One

Carnosic acid biosynthesis elucidated by a synthetic biology platform

(2016) Codruta Ignea et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced limonene production in cyanobacteria reveals photosynthesis limitations

(2016) Xin Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Unique attributes of cyanobacterial metabolism revealed by improved genome-scale metabolic modeling and essential gene analysis

(2016) Jared T. Broddrick et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Tapping the Unused Potential of Photosynthesis with a Heterologous Electron Sink

(2016) Adokiye Berepiki et al.   ACS Synthetic Biology

Limitations in the biosynthesis of fucoxanthin as targets for genetic engineering in Phaeodactylum tricornutum

(2015) Ulrike Eilers et al.   JOURNAL OF APPLIED PHYCOLOGY

Reconstructing the chemical diversity of labdane-type diterpene biosynthesis in yeast

(2015) Codruta Ignea et al.   METABOLIC ENGINEERING

Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae

(2015) Wenping Xie et al.   METABOLIC ENGINEERING

Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration

(2015) Chiam Yu Ng et al.   METABOLIC ENGINEERING

Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase

(2015) Codruta Ignea et al.   METABOLIC ENGINEERING

Cyanobacteria as photosynthetic biocatalysts: a systems biology perspective

(2015) Steinn Gudmundsson et al.   Molecular BioSystems

Distributing a metabolic pathway among a microbial consortium enhances production of natural products

(2015) Kang Zhou et al.   NATURE BIOTECHNOLOGY

Flux balance analysis of primary metabolism in the diatomPhaeodactylum tricornutum

(2015) Joomi Kim et al.   PLANT JOURNAL

The essential gene set of a photosynthetic organism

(2015) Benjamin E. Rubin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

(2015) Elias Englund et al.   ACS Synthetic Biology

Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

(2015) Thiyagarajan Gnanasekaran et al.   Journal of Biological Engineering

To Gibberellins and Beyond! Surveying the Evolution of (Di)Terpenoid Metabolism

(2014) Jiachen Zi et al.   Annual Review of Plant Biology

Genetically engineering cyanobacteria to convert CO2, water, and light into the long-chain hydrocarbon farnesene

(2014) Charles Halfmann et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways

(2014) Jianming Yang et al.   Microbial Cell Factories

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Tracking the sterol biosynthesis pathway of the diatomPhaeodactylum tricornutum

(2014) Michele Fabris et al.   NEW PHYTOLOGIST

Toward a photosynthetic microbial platform for terpenoid engineering

(2014) Fiona K. Davies et al.   PHOTOSYNTHESIS RESEARCH

Metabolic engineering of volatile isoprenoids in plants and microbes

(2014) CLAUDIA E. VICKERS et al.   PLANT CELL AND ENVIRONMENT

Regulation of β-phellandrene synthase gene expression, recombinant protein accumulation, and monoterpene hydrocarbons production in Synechocystis transformants

(2014) Cinzia Formighieri et al.   PLANTA

Production of Squalene in Synechocystis sp. PCC 6803

(2014) Elias Englund et al.   PLoS One

Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16  hydroxylase from Bupleurum falcatum

(2014) T. Moses et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering Acetyl Coenzyme A Supply: Functional Expression of a Bacterial Pyruvate Dehydrogenase Complex in the Cytosol of Saccharomyces cerevisiae

(2014) B. U. Kozak et al.   mBio

Paradigm of Monoterpene (β-phellandrene) Hydrocarbons Production via Photosynthesis in Cyanobacteria

(2013) Fiona K. Bentley et al.   BioEnergy Research

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production

(2013) Jorge Alonso-Gutierrez et al.   METABOLIC ENGINEERING

Experimental evidence and isotopomer analysis of mixotrophic glucose metabolism in the marine diatom Phaeodactylum tricornutum

(2013) Yuting Zheng et al.   Microbial Cell Factories

Heterologous Expression of the Mevalonic Acid Pathway in Cyanobacteria Enhances Endogenous Carbon Partitioning to Isoprene

(2013) Fiona K. Bentley et al.   Molecular Plant

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Synthetic non-oxidative glycolysis enables complete carbon conservation

(2013) Igor W. Bogorad et al.   NATURE

High-level diterpene production by transient expression in Nicotiana benthamiana

(2013) Kathleen Brückner et al.   Plant Methods

Gene Discovery of Modular Diterpene Metabolism in Nonmodel Systems

(2013) P. Zerbe et al.   PLANT PHYSIOLOGY

What makes a P450 tick?

(2013) Andrew W. Munro et al.   TRENDS IN BIOCHEMICAL SCIENCES

Programming adaptive control to evolve increased metabolite production

(2013) Howard H. Chou et al.   Nature Communications

Redirecting Photosynthetic Electron Flow into Light-Driven Synthesis of Alternative Products Including High-Value Bioactive Natural Compounds

(2013) Lærke Münter Lassen et al.   ACS Synthetic Biology

Engineering Monoterpene Production in Yeast Using a Synthetic Dominant Negative Geranyl Diphosphate Synthase

(2013) Codruta Ignea et al.   ACS Synthetic Biology

Rerouting Carbon Flux To Enhance Photosynthetic Productivity

(2012) Daniel C. Ducat et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Isoprene Production Via the Mevalonic Acid Pathway in Escherichia coli (Bacteria)

(2012) Andreas Zurbriggen et al.   BioEnergy Research

Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii

(2012) Nanette R Boyle et al.   BMC Systems Biology

Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production

(2012) Bilge Özaydın et al.   METABOLIC ENGINEERING

Microbial engineering for the production of advanced biofuels

(2012) Pamela P. Peralta-Yahya et al.   NATURE

The metabolic blueprint of Phaeodactylum tricornutum reveals a eukaryotic Entner-Doudoroff glycolytic pathway

(2012) Michele Fabris et al.   PLANT JOURNAL

Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E. coli

(2012) Jianming Yang et al.   PLoS One

Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway

(2011) Yaru Zhao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Metabolic modeling of Chlamydomonas reinhardtii: energy requirements for photoautotrophic growth and maintenance

(2011) Anna M. J. Kliphuis et al.   JOURNAL OF APPLIED PHYCOLOGY

Optimization of a heterologous mevalonate pathway through the use of variant HMG-CoA reductases

(2011) Suzanne M. Ma et al.   METABOLIC ENGINEERING

Metabolic network reconstruction of Chlamydomonas offers insight into light-driven algal metabolism

(2011) R. L. Chang et al.   Molecular Systems Biology

The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom

(2011) Feng Chen et al.   PLANT JOURNAL

Engineering cyanobacteria to generate high-value products

(2011) Daniel C. Ducat et al.   TRENDS IN BIOTECHNOLOGY

Grand Challenge Commentary: Chassis cells for industrial biochemical production

(2010) Claudia E Vickers et al.   Nature Chemical Biology

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

ChlamyCyc: an integrative systems biology database and web-portal for Chlamydomonas reinhardtii

(2009) Patrick May et al.   BMC GENOMICS

Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

(2009) Pia Lindberg et al.   METABOLIC ENGINEERING

A unified mechanism of action for volatile isoprenoids in plant abiotic stress

(2009) Claudia E Vickers et al.   Nature Chemical Biology

Gene silencing in the marine diatom Phaeodactylum tricornutum

(2009) Valentina De Riso et al.   NUCLEIC ACIDS RESEARCH

Reconstruction of a bacterial isoprenoid biosynthetic pathway inSaccharomyces cerevisiae

(2008) Jérôme Maury et al.   FEBS LETTERS

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL

A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis

(2008) Peter G. Kroth et al.   PLoS One