Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion

Genetically Encoded Glutamate Indicators with Altered Color and Topology

(2018) Jiahui Wu et al.   ACS Chemical Biology

Development of novel metabolite-responsive transcription factors via transposon-mediated protein fusion

(2018) Andrew K D Younger et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Protein Engineering Strategies to Expand CRISPR-Cas9 Applications

(2018) Lucas F. Ribeiro et al.   International Journal of Genomics

Smart insulin: redesign could end hypoglycemia risk

(2018) Brian Owens   NATURE BIOTECHNOLOGY

The Application of Whole Cell-Based Biosensors for Use in Environmental Analysis and in Medical Diagnostics

(2017) Qingyuan Gui et al.   SENSORS

Small Molecule-Induced Domain Swapping as a Mechanism for Controlling Protein Function and Assembly

(2017) Joshua M. Karchin et al.   Scientific Reports

Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering

(2017) Yang Liu et al.   Frontiers in Microbiology

A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination

(2016) Lucas Ferreira Ribeiro et al.   Biotechnology for Biofuels

SnapShot: Timescales in Cell Biology

(2016) Maya Shamir et al.   CELL

Introduction to Protein Ensembles and Allostery

(2016) Ruth Nussinov   CHEMICAL REVIEWS

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

The interplay between effector binding and allostery in an engineered protein switch

(2016) Jay H. Choi et al.   PROTEIN SCIENCE

Rapid construction of metabolite biosensors using domain-insertion profiling

(2016) Dana C. Nadler et al.   Nature Communications

Engineering Modular Biosensors to Confer Metabolite-Responsive Regulation of Transcription

(2016) Andrew K. D. Younger et al.   ACS Synthetic Biology

Application of genetically engineered microbial whole-cell biosensors for combined chemosensing

(2015) Wei He et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Experimental Strategies for Functional Annotation and Metabolism Discovery: Targeted Screening of Solute Binding Proteins and Unbiased Panning of Metabolomes

(2015) Matthew W. Vetting et al.   BIOCHEMISTRY

Electrochemical activation of engineered protein switches

(2015) Jay H. Choi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enzymatic protein switches built from paralogous input domains

(2015) Jennifer Tullman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

(2015) Lucas Ferreira Ribeiro et al.   Biotechnology for Biofuels

Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein

(2015) Brennal Pierre et al.   CHEMBIOCHEM

Engineered Domain Swapping as an On/Off Switch for Protein Function

(2015) Jeung-Hoi Ha et al.   CHEMISTRY & BIOLOGY

Optogenetic Inhibitor of the Transcription Factor CREB

(2015) Ahmed M. Ali et al.   CHEMISTRY & BIOLOGY

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Synthetic protein switches: design principles and applications

(2015) Viktor Stein et al.   TRENDS IN BIOTECHNOLOGY

Design of protein switches based on an ensemble model of allostery

(2015) Jay H. Choi et al.   Nature Communications

A general strategy to construct small molecule biosensors in eukaryotes

(2015) Justin Feng et al.   eLife

Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes

(2014) Matthew F Copeland et al.   CURRENT OPINION IN BIOTECHNOLOGY

The ensemble nature of allostery

(2014) Hesam N. Motlagh et al.   NATURE

Rational Design of a Fusion Protein to Exhibit Disulfide-Mediated Logic Gate Behavior

(2014) Jay H. Choi et al.   ACS Synthetic Biology

Systematic Transfer of Prokaryotic Sensors and Circuits to Mammalian Cells

(2014) Brynne C. Stanton et al.   ACS Synthetic Biology

Rational design of FRET sensor proteins based on mutually exclusive domain interactions

(2013) Maarten Merkx et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The High-Affinity Maltose Switch MBP317-347 has Low Affinity for Glucose: Implications for Targeting Tumors with Metabolically Directed Enzyme Prodrug Therapy

(2013) Gilmer Valdes et al.   Chemical Biology & Drug Design

Biosensors: sense and sensibility

(2013) Anthony P. F. Turner   CHEMICAL SOCIETY REVIEWS

Determinants of specificity in two-component signal transduction

(2013) Anna I Podgornaia et al.   CURRENT OPINION IN MICROBIOLOGY

The Designability of Protein Switches by Chemical Rescue of Structure: Mechanisms of Inactivation and Reactivation

(2013) Yan Xia et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Computational design of ligand-binding proteins with high affinity and selectivity

(2013) Christine E. Tinberg et al.   NATURE

An optimized fluorescent probe for visualizing glutamate neurotransmission

(2013) Jonathan S Marvin et al.   NATURE METHODS

Rational design of a ligand-controlled protein conformational switch

(2013) O. Dagliyan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Non-allosteric enzyme switches possess larger effector-induced changes in thermodynamic stability than their non-switch analogs

(2013) Jay H. Choi et al.   PROTEIN SCIENCE

Protein Logic: A Statistical Mechanical Study of Signal Integration at the Single-Molecule Level

(2012) Wiet de Ronde et al.   BIOPHYSICAL JOURNAL

Designing Allosteric Control into Enzymes by Chemical Rescue of Structure

(2012) Katelyn Deckert et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A designed bifunctional laccase/ -1,3-1,4-glucanase enzyme shows synergistic sugar release from milled sugarcane bagasse

(2012) G. P. Furtado et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Protein switches identified from diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA

(2011) Jennifer Tullman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Hot Spots for Allosteric Regulation on Protein Surfaces

(2011) Kimberly A. Reynolds et al.   CELL

Engineering Bifunctional Laccase-Xylanase Chimeras for Improved Catalytic Performance

(2011) Lucas F. Ribeiro et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of the Escherichia coli Phosphonate Binding Protein PhnD and Rationally Optimized Phosphonate Biosensors

(2011) Ismael Alicea et al.   JOURNAL OF MOLECULAR BIOLOGY

In Vitro Recombination of Non-Homologous Genes Can Result in Gene Fusions that Confer a Switching Phenotype to Cells

(2011) Richard A. Heins et al.   PLoS One

A protein therapeutic modality founded on molecular regulation

(2011) C. M. Wright et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A genetically encoded, high-signal-to-noise maltose sensor

(2011) Jonathan S. Marvin et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Design and application of genetically encoded biosensors

(2011) Amy E. Palmer et al.   TRENDS IN BIOTECHNOLOGY

Biosensors and their applications in microbial metabolic engineering

(2011) Fuzhong Zhang et al.   TRENDS IN MICROBIOLOGY

Domain assignment for protein structures using a consensus approach: Characterization and analysis

(2010) Susan Jones et al.   PROTEIN SCIENCE

An Ensemble View of Allostery

(2010) V. J. Hilser   SCIENCE

Designs and applications of fluorescent protein-based biosensors

(2009) Andreas Ibraheem et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

An externally tunable bacterial band-pass filter

(2009) T. Sohka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzyme stabilization by domain insertion into a thermophilic protein

(2009) C.-S. Kim et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The Evolutionary Mechanics of Domain Organization in Proteomes and the Rise of Modularity in the Protein World

(2009) Minglei Wang et al.   STRUCTURE

GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor

(2008) Hitomi Takanaga et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Effect of Interdomain Linker Length on an Antagonistic Folding–Unfolding Equilibrium between Two Protein Domains

(2008) Thomas A. Cutler et al.   JOURNAL OF MOLECULAR BIOLOGY

Allosteric regulation and catalysis emerge via a common route

(2008) Nina M Goodey et al.   Nature Chemical Biology

Surface Sites for Engineering Allosteric Control in Proteins

(2008) J. Lee et al.   SCIENCE

Structure Prediction of Domain Insertion Proteins from Structures of Individual Domains

(2008) Monica Berrondo et al.   STRUCTURE