Rapid construction of metabolite biosensors using domain-insertion profiling

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Enzymatic protein switches built from paralogous input domains

(2015) Jennifer Tullman et al.   BIOTECHNOLOGY AND BIOENGINEERING

The ensemble nature of allostery

(2014) Hesam N. Motlagh et al.   NATURE

Computational design of ligand-binding proteins with high affinity and selectivity

(2013) Christine E. Tinberg et al.   NATURE

Pfam: the protein families database

(2013) Robert D. Finn et al.   NUCLEIC ACIDS RESEARCH

Quantitative Imaging with Fluorescent Biosensors

(2012) Sakiko Okumoto et al.   Annual Review of Plant Biology

Cellular Metabolism and Disease: What Do Metabolic Outliers Teach Us?

(2012) Ralph J. DeBerardinis et al.   CELL

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

(2012) J. Akerboom et al.   JOURNAL OF NEUROSCIENCE

Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing

(2012) Timothy A Whitehead et al.   NATURE BIOTECHNOLOGY

Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity

(2011) He Li et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

A genetically encoded, high-signal-to-noise maltose sensor

(2011) Jonathan S. Marvin et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Deep mutational scanning: assessing protein function on a massive scale

(2011) Carlos L. Araya et al.   TRENDS IN BIOTECHNOLOGY

Systems biology of microbial metabolism

(2010) Matthias Heinemann et al.   CURRENT OPINION IN MICROBIOLOGY

Protein dynamics and allostery: an NMR view

(2010) Shiou-Ru Tzeng et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

High-resolution mapping of protein sequence-function relationships

(2010) Douglas M Fowler et al.   NATURE METHODS

Differential expression analysis for sequence count data

(2010) Simon Anders et al.   GENOME BIOLOGY

Genetically encoded biosensors based on engineered fluorescent proteins

(2009) Wolf B. Frommer et al.   CHEMICAL SOCIETY REVIEWS

Designs and applications of fluorescent protein-based biosensors

(2009) Andreas Ibraheem et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Trehalose Metabolism and Signaling

(2008) Matthew J. Paul et al.   Annual Review of Plant Biology

Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design

(2008) Jasper Akerboom et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Linking the functions of unrelated proteins using a novel directed evolution domain insertion method

(2008) Wayne R. Edwards et al.   NUCLEIC ACIDS RESEARCH

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One

Allostery and cooperativity revisited

(2008) Qiang Cui et al.   PROTEIN SCIENCE

Surface Sites for Engineering Allosteric Control in Proteins

(2008) J. Lee et al.   SCIENCE