Review
Pharmacology & Pharmacy
Jie Li, Joachim Justad Raise, Maomao He, Riddha Das, Niren Murthy
Summary: Genome-editing tools like CRISPR have revolutionized various fields, but the low delivery efficiency of genome editing proteins in vivo remains a major challenge, with the most successful strategy currently involving potentially immunogenic viral vectors.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Peng Yang, Athena Yue-Tung Lee, Jingjing Xue, Shih-Jie Chou, Calvin Lee, Patrick Tseng, Tiffany X. Zhang, Yazhen Zhu, Junseok Lee, Shih-Hwa Chiou, Hsian-Rong Tseng
Summary: The CRISPR/Cas9 genome editing system has revolutionized the field of therapeutic applications for genetic diseases and cancers. Efficient delivery of the system in vivo remains a significant challenge. Non-viral nano-vectors offer advantages over viral vectors and can be used to deliver CRISPR/Cas9 cargoes for targeted gene editing. The article highlights recent advances in nano-vector delivery for treating cancers and genetic diseases and proposes strategies for future development.
Review
Chemistry, Multidisciplinary
Weiqi Cai, Tianli Luo, Lanqun Mao, Ming Wang
Summary: Recent innovations in genome editing have led to precise manipulation of genetic information in mammals using stimuli-responsive nanoparticles for the delivery and release of CRISPR/Cas9 genome editing machinery. These approaches can utilize biological signals or external signals to achieve improved genome editing outcomes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Biochemistry & Molecular Biology
Muhammad Naeem, Mubasher Zahir Hoque, Muhammad Ovais, Chanbasha Basheer, Irshad Ahmad
Summary: The innovative CRISPR-Cas technology has enabled genetic engineers to manipulate genomes effectively, but barriers in clinical applications remain, including efficient delivery and off-target effects. Various viral and non-viral vectors are designed to address these issues, but challenges such as immune response and lack of specificity persist.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Anchu Viswan, Ayana Yamagishi, Masamichi Hoshi, Yuichi Furuhata, Yoshio Kato, Natsumi Makimoto, Toshihiro Takeshita, Takeshi Kobayashi, Futoshi Iwata, Mitsuhiro Kimura, Takeshi Yoshizumi, Chikashi Nakamura
Summary: This article introduces a novel microneedle array-based delivery system for genome-editing proteins, which can control the point of entry precisely and successfully deliver the proteins into plant tissues.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Min Qiu, Zachary Glass, Jinjin Chen, Mary Haas, Xin Jin, Xuewei Zhao, Xuehui Rui, Zhongfeng Ye, Yamin Li, Feng Zhang, Qiaobing Xu
Summary: Loss-of-function mutations in Angiopoietin-like 3 (Angptl3) are linked to reduced blood lipid levels, making it a potential therapeutic target for lipid metabolism disorders. A lipid nanoparticle delivery system successfully edited Angptl3 gene in vivo, leading to significant reductions in lipid levels without off-target effects or toxicity, highlighting the potential of this platform for safe and effective gene editing therapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biotechnology & Applied Microbiology
Namita Khajanchi, Krishanu Saha
Summary: Biomedical research has been revolutionized by CRISPR-Cas systems, but there are still challenges in terms of editing efficiency and safety. Recent studies have made progress in controlling the expression and activity of Cas systems, including the use of small molecules as regulatory switches, which have the potential to improve the efficiency of in vivo genome editing and reduce adverse effects.
Review
Urology & Nephrology
Yoshiharu Muto, Benjamin D. Humphreys
Summary: Lineage tracing was originally developed to identify all progeny of a single cell during morphogenesis, but has since been applied to organ homeostasis and injury recovery. Modern techniques rely on reporter gene expression induced by cell-specific DNA recombination, and recent advances include intersectional genetics and sequencing-based genomic lineage tracing.
KIDNEY INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin
Summary: Lactobacillus, important in food production and probiotics, can benefit from CRISPR/Cas9-based genome editing to improve strain efficiency and achieve traceless genome modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Chao Zhang, Shanhe Liu, Xuan Li, Ruixuan Zhang, Jun Li
Summary: CRISPR/Cas-based genome editing technologies have revolutionized plant science by allowing precise manipulation of plant genomes. Virus-induced genome editing (VIGE) has emerged as a powerful method for delivering CRISPR/Cas reagents into plant cells, with high editing efficiency and simplified process. This article provides an overview of CRISPR/Cas-based genome editing and focuses on the VIGE systems, types of viruses used for CRISPR/Cas9 cassette delivery, recent applications, and challenges and potential of VIGE in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Tao Yang, Haobo Han, Ying Chen, Liu Yang, Rachael Parker, Yamin Li, David L. Kaplan, Qiaobing Xu
Summary: The study demonstrates that synthetic lipid nanoparticles and protein cargo nanocomplexes can successfully cross the intestinal epithelial layer and deliver the protein cargo to targeted cells. These nanocomplexes can penetrate intestinal monolayer cells by temporarily interrupting tight junctions between cells, inducing gene recombination inside the cells below.
BIOACTIVE MATERIALS
(2021)
Article
Plant Sciences
Yuichi Furuhata, Emiko Egi, Tomi Murakami, Yoshio Kato
Summary: The Cre/lox recombination system is a powerful tool for studying gene function in different cell types and organisms. In this study, we successfully delivered Cre protein into tobacco-derived BY-2 cells with intact cell walls using electroporation. This method provides useful insights for genome engineering in various plant cells with different types of cell walls.
Review
Medicine, Research & Experimental
Asma Ghaemi, Elnaz Bagheri, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona Alibolandi
Summary: The prokaryotic CRISPR-Cas systems serve as a revolutionary genome editing tool for modifying, visualizing, and identifying DNA and RNA sequences in live cells of various species. Besides genome editing, the DNA-targeting modifications by CRISPR-Cas offer opportunities for diagnosis, therapy, and genetic disorders investigation. Preclinical studies and CRISPR-Cas9-based therapeutics may encounter challenges, but there is potential for its development in cancer therapy.
Review
Biochemistry & Molecular Biology
Chenya Zhuo, Jiabin Zhang, Jung-Hwan Lee, Ju Jiao, Du Cheng, Li Liu, Hae-Won Kim, Yu Tao, Mingqiang Li
Summary: The CRISPR/Cas9 gene editing technology shows promise in treating genetic and infectious diseases but faces challenges in precise editing. Emerging strategies to regulate CRISPR/Cas9 activity have shown potential in improving spatiotemporal control, but obstacles and limitations remain for clinical translation. Further exploration is needed to enhance the spatiotemporal operability of CRISPR/Cas9.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
