Anti-LRP5/6 VHHs promote differentiation of Wnt-hypersensitive intestinal stem cells

DIALS: implementation and evaluation of a new integration package

(2018) Graeme Winter et al.   Acta Crystallographica Section D-Structural Biology

Inhibition of HER3 activation and tumor growth with a human antibody binding to a conserved epitope formed by domain III and IV

(2017) Lisa C. Schmitt et al.   mAbs

Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling

(2017) Claudia Y. Janda et al.   NATURE

Interplay between metabolic identities in the intestinal crypt supports stem cell function

(2017) Maria J. Rodríguez-Colman et al.   NATURE

Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics

(2017) Peter Bannas et al.   Frontiers in Immunology

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids

(2016) Amanda Andersson-Rolf et al.   DEVELOPMENTAL BIOLOGY

Novel Bispecific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-Induced Wnt Signaling and Tumor Growth

(2016) H. Jackson et al.   MOLECULAR CANCER RESEARCH

Wnt signaling in cancer

(2016) T Zhan et al.   ONCOGENE

Identification of Novel Candidate Genes for Early-Onset Colorectal Cancer Susceptibility

(2016) Richarda M. de Voer et al.   PLoS Genetics

Nanobodies as Versatile Tools to Understand, Diagnose, Visualize and Treat Cancer

(2016) Isabel Van Audenhove et al.   EBioMedicine

Control of Wnt Receptor Turnover by R-spondin-ZNRF3/RNF43 Signaling Module and Its Dysregulation in Cancer

(2016) Huai-Xiang Hao et al.   Cancers

Targeting Wnt signaling in colorectal cancer. A Review in the Theme: Cell Signaling: Proteins, Pathways and Mechanisms

(2015) Laura Novellasdemunt et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Nanobody-based cancer therapy of solid tumors

(2015) Marta Kijanka et al.   Nanomedicine

Targeting PTPRK-RSPO3 colon tumours promotes differentiation and loss of stem-cell function

(2015) Elaine E. Storm et al.   NATURE

Wnt addiction of genetically defined cancers reversed by PORCN inhibition

(2015) B Madan et al.   ONCOGENE

Porcupine inhibitor suppresses paracrine Wnt-driven growth ofRnf43;Znrf3-mutant neoplasia

(2015) Bon-Kyoung Koo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stem Cells Marked by the R-Spondin Receptor LGR5

(2014) Bon-Kyoung Koo et al.   GASTROENTEROLOGY

The R-spondin/Lgr5/Rnf43 module: regulator of Wnt signal strength

(2014) W. de Lau et al.   GENES & DEVELOPMENT

Comprehensive molecular characterization of gastric adenocarcinoma

(2014) Adam J. Bass et al.   NATURE

WNT signaling in bone development and homeostasis

(2014) Zhendong Zhong et al.   Wiley Interdisciplinary Reviews-Developmental Biology

How good are my data and what is the resolution?

(2013) Philip R. Evans et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny

(2013) Xiaolei Yin et al.   NATURE METHODS

Adult intestinal stem cells: critical drivers of epithelial homeostasis and regeneration

(2013) Nick Barker   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Antagonistic TNF Receptor One-Specific Antibody (ATROSAB): Receptor Binding and In Vitro Bioactivity

(2013) Fabian Richter et al.   PLoS One

Selection of a high-affinity WW domain against the extracellular region of VEGF receptor isoform-2 from a combinatorial library using CIS display

(2013) S. Patel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Structural Evaluation of EGFR Inhibition Mechanisms for Nanobodies/VHH Domains

(2013) Karl R. Schmitz et al.   STRUCTURE

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Single-cycle kinetic analysis of ternary DNA complexes by surface plasmon resonance on a decaying surface

(2012) William Palau et al.   BIOCHIMIE

Redundant Sources of Wnt Regulate Intestinal Stem Cells and Promote Formation of Paneth Cells

(2012) Henner F. Farin et al.   GASTROENTEROLOGY

ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner

(2012) Huai-Xiang Hao et al.   NATURE

Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors

(2012) Bon-Kyoung Koo et al.   NATURE

The complex world of WNT receptor signalling

(2012) Christof Niehrs   NATURE REVIEWS MOLECULAR CELL BIOLOGY

LRP5 and LRP6 in development and disease

(2012) Danese M. Joiner et al.   TRENDS IN ENDOCRINOLOGY AND METABOLISM

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structural Basis of Wnt Signaling Inhibition by Dickkopf Binding to LRP5/6

(2011) Victoria E. Ahn et al.   DEVELOPMENTAL CELL

Structural and Functional Studies of LRP6 Ectodomain Reveal a Platform for Wnt Signaling

(2011) Shuo Chen et al.   DEVELOPMENTAL CELL

Crystal structures of the extracellular domain of LRP6 and its complex with DKK1

(2011) Zhihong Cheng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dissecting Molecular Differences between Wnt Coreceptors LRP5 and LRP6

(2011) Bryan T. MacDonald et al.   PLoS One

Whole-exome sequencing of neoplastic cysts of the pancreas reveals recurrent mutations in components of ubiquitin-dependent pathways

(2011) J. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Wnt Antagonists Bind through a Short Peptide to the First β-Propeller Domain of LRP5/6

(2011) Eric Bourhis et al.   STRUCTURE

Reconstitution of a Frizzled8·Wnt3a·LRP6 Signaling Complex Reveals Multiple Wnt and Dkk1 Binding Sites on LRP6

(2010) Eric Bourhis et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Loss of the Tumor Suppressor CYLD Enhances Wnt/β-Catenin Signaling through K63-Linked Ubiquitination of Dvl

(2010) Daniele V.F. Tauriello et al.   MOLECULAR CELL

Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

(2010) Toshiro Sato et al.   NATURE

Wnt Isoform-Specific Interactions with Coreceptor Specify Inhibition or Potentiation of Signaling by LRP6 Antibodies

(2010) Yan Gong et al.   PLoS One

Inhibition of tumorigenesis driven by different Wnt proteins requires blockade of distinct ligand-binding regions by LRP6 antibodies

(2010) S. A. Ettenberg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation

(2009) Norihisa Yasui et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The First Propeller Domain of LRP6 Regulates Sensitivity to DKK1

(2009) Minke E. Binnerts et al.   MOLECULAR BIOLOGY OF THE CELL

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Small molecule–mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer

(2009) Baozhi Chen et al.   Nature Chemical Biology

An in vitro selection strategy for conferring protease resistance to ligand binding peptides

(2009) B. Eldridge et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Wnt-mediated self-renewal of neural stem/progenitor cells

(2008) M. Y. S. Kalani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA