Article
Biochemistry & Molecular Biology
Jae-Won Hyun, Kibin Lee, Ji-Hun Kim, Dae-Won Sim, Kyu-Tae Byun, Seung-Jae Jung, Jin Chul Paeng, Tae-Bong Kang, Jooho Park, Chan-Gil Kim, Hyung-Sik Won
Summary: The study demonstrates a method for extracellular production of anti-HER2 in Escherichia coli via nonpeptide-guided secretion, enabling purification of the intact protein with high affinity and dissociation constant for potential therapeutic or diagnostic applications in cancer.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yui Nakata, Yuuto Kitazaki, Hitomi Kanaoka, Erika Shingen, Rina Uehara, Kunihiro Hongo, Yasushi Kawata, Tomohiro Mizobata
Summary: The molecular chaperones HdeA and HdeB help protect client proteins from acid denaturation in E. coli. Both proteins can form fibrils at low pH, but the fibrils can be resolubilized at neutral pH. However, extended incubation at low pH can lead to the formation of irreversible fibrils. The secondary structure of HdeB fibrils remained alpha helical regardless of the conditions under which the fibrils were formed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Paola Munoz-Lopez, Rosa Maria Ribas-Aparicio, Elayne Irene Becerra-Baez, Karla Fraga-Perez, Luis Fernando Flores-Martinez, Armando Alfredo Mateos-Chavez, Rosendo Luria-Perez
Summary: Recombinant antibody fragments, particularly the single-chain fragment variable (scFv), have shown great potential in cancer diagnosis and therapy with their high specificity, affinity, and ability to penetrate tumor tissues. They offer a promising alternative for patients who fail to respond to conventional treatments.
Article
Food Science & Technology
Long Li, Xiaoqing Wang, Ren Hou, Yulian Wang, Xu Wang, Changqing Xie, Yushuang Chen, Shuangmin Wu, Dapeng Peng
Summary: The study generated a single-chain fragment variable (scFv) for the detection of macrolide antibiotics (MAs) residues through biopanning and comparative analysis, revealing that mutations in the FR framework region can affect the active pocket and recognition ability of the antibody. Through homology modeling and molecular docking, the binding sites between the optimal MA-specific scFv candidate and MA were identified.
Article
Immunology
Chi-Hsin Lee, Chu-Hsuan Tsai, Sy-Jye Leu, Ko-Jiunn Liu, Wei-Chu Wang, Bor-Yu Tsai, Liao-Chun Chiang, Yan-Chiao Mao, Nhlanhla Benedict Dlamini, Chen-Hsin Tsai, Yi-Yuan Yang
Summary: This study demonstrates that overexpression of human alpha-enolase (hEno1) is closely associated with a wide range of cancers and poor prognosis, making it a significant biomarker and therapeutic target. Antibodies derived from hEno1-immunized chickens show a specific humoral response. Phage display technology was used to construct antibody libraries, which were able to identify three distinguish antigenic epitopes on the hEno1 protein. Selected anti-hEno1 antibodies showed potential to inhibit the growth and migration of lung cancer cells. These findings suggest that chicken-derived antibodies could be valuable for the diagnosis and treatment of lung cancer patients with high hEno1 expression levels.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chihiro Aikawa, Kiyosumi Kawashima, Chihiro Fukuzaki, Makoto Nakakido, Kazunori Murase, Takashi Nozawa, Kouhei Tsumoto, Ichiro Nakagawa
Summary: Group A Streptococcus causes various diseases, with toxins like streptolysin O playing a key role in severe infections. Research has identified an anti-SLO single-chain variable fragment, SLO-I4, which effectively inhibits GAS-induced cell lysis and may be beneficial in combating GAS infections.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yoshimi Shimizu, Takehiro Shinoda, Yoshitaka Shirasago, Masuo Kondoh, Naoko Shinya, Kentaro Hanada, Kiyohito Yagi, Tetsuro Suzuki, Takaji Wakita, Tomomi Kimura-Someya, Mikako Shirouzu, Masayoshi Fukasawa
Summary: The study demonstrates that the conversion of monoclonal antibodies to monovalent antigen-binding fragments and single-chain variable fragment antibodies maintains high binding specificity and affinity, and also shows inhibition of HCV infection. These functional monovalent OCLN-binding probes have potential as drug candidates and tools for studies of OCLN in biology and pharmaceuticals.
Article
Biochemical Research Methods
Qiyi He, Li Yang, Mingxia Lin, Huiyi Yang, Xiping Cui, Mark R. McCoy, Bruce D. Hammock, Yanxiong Fang, Suqing Zhao
Summary: Ferritin is a serological biomarker for liver diseases and cancers and can be used as an important tool in health diagnosis. Researchers constructed a phage library and obtained a highly selective and clinically applicable dual functional immunoprobe for rapid, reliable, and highly sensitive ferritin detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Fisheries
Siyou Huang, Yujia Wu, Lianpan Su, Taowen Su, Qiong Zhou, Jing Zhang, Zhiying Zhao, Shaoping Weng, Jianguo He, Junfeng Xie
Summary: In this study, an anti-NNV strategy using a single-chain variable fragment (ScFv) antibody named Sc3F9 was applied to eliminate the possibility of NNV infection during fertilization. The antibody showed broad neutralizing capacity and stable use in seawater, making it suitable for NNV decontamination, especially in obtaining NNV-free grouper eggs. Furthermore, the antibody demonstrated strong neutralization of OGNNV.
Article
Biotechnology & Applied Microbiology
Nguyen Hieu Nghia, Yoichi Kumada, Michimasa Kishimoto, Jun-ichi Horiuchi
Summary: The study successfully increased the production and solubility of anti-CRP scFv using the DO-stat fed-batch culture, while effectively suppressing the formation of inclusion bodies.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Philipp Diebolder, Cedric Mpoy, Jalen Scott, Truc T. Huynh, Ryan Fields, Dirk Spitzer, Nilantha Bandara, Buck E. Rogers
Summary: Glycoprotein CD44 and its splice variants are overexpressed in many cancers and cancer stem cells, with hyaluronic acid binding to CD44 to activate cell signaling pathways. Developing a CD44-specific antibody fragment for imaging CD44-positive cancers using PET shows potential clinical utility in cancer therapy.
JOURNAL OF NUCLEAR MEDICINE
(2021)
Article
Biochemical Research Methods
Manman Lin, Yuan Liu, Xiao Zhang, Jianfeng Zhong, Xiaodan Hu, Chongxin Xu, Yajing Xie, Cunzheng Zhang, Ying Liang, Xianjin Liu, Johnson Lin
Summary: The study obtained seven anti-Cry2Aa toxin anti-idiotypic scFv antibodies from a phage-displayed mutant library, with mutant 2-12B showing the best binding ability. 2-12B and Cry2Aa toxin shared a potential receptor of polycalin in P. xylostella BBMVs and had seven common binding amino acid residues in polycalin. Insect bioassay results indicated that 2-12 had insecticidal efficacy against P. xylostella larvae, suggesting that the 2-12B antibody partially mimics the structure and function of Cry2Aa toxin.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Jialiang Zhao, Jingjing Xu, Tianbin Yang, Xinze Yu, Cheng Cheng, Tong Zhang, Ze Ren, Na Li, Fang Yang, Guiying Li
Summary: This study successfully expressed and purified a novel anti-CDK4 scFv antibody that can specifically recognize and interact with endogenous CDK4 in tumor cells, suggesting a promising new tool for CDK4-targeted diagnosis, prognosis, and treatment of various cancer types.
Article
Immunology
Chia-Yu Chang, Yong-Sheng Wang, Jou-Fei Wu, Tzu-Jing Yang, Yen-Chen Chang, Chanhee Chae, Hui-Wen Chang, Shang-Te Danny Hsu
Summary: The emergence of the G2 genotype and re-emergence of G1 porcine epidemic diarrhea virus have caused severe economic impacts, and the development of new variant vaccines has been challenging. The scFv specific to G2b PEDV could potentially be used as a therapeutic candidate against the virulent PEDV.
Article
Biophysics
Mukund Khanwalker, Rinko Fujita, Jinhee Lee, Ellie Wilson, Kohei Ito, Ryutaro Asano, Kazunori Ikebukuro, Jeffrey LaBelle, Koji Sode
Summary: This study focuses on utilizing anti-insulin single chain variable fragment as a biosensing molecule combined with single frequency faradaic electrochemical impedance spectroscopy to develop a sensor for efficient insulin detection. By comparing two immobilization methods, the study demonstrates the sensor's effectiveness and determines the optimal measurement frequency.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Agricultural Engineering
Xin Jiat Lee, Pau Loke Show, Tomohisa Katsuda, Wei-Hsin Chen, Jo-Shu Chang
BIORESOURCE TECHNOLOGY
(2018)
Meeting Abstract
Biochemical Research Methods
H. Yamaji, T. Tanijima, T. Matsuda, K. Masumi-Koizumi, T. Katsuda
Article
Cell Biology
Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2019)
Article
Engineering, Chemical
Hui Yi Leong, Chien Wei Ooi, Chung Lim Law, Advina Lizah Julkifle, Tomohisa Katsuda, Pau Loke Show
SEPARATION AND PURIFICATION TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Yu Mizote, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Ryou Nakanuma, Kyoko Masumi-Koizumi, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji
Summary: This study found that adding autophagy inducers rapamycin or everolimus can prolong the growth cycle of insect cells, improve survival rates, and increase the production of secreted antibodies, potentially through the autophagy pathway.
Article
Biotechnology & Applied Microbiology
Yuki Ohmuro-Matsuyama, Keiko Gomi, Takuya Shimoda, Hideki Yamaji, Hiroshi Ueda
Summary: The study developed a protein-protein interaction assay, FlimPIA, based on mutant firefly luciferases, improving the stability and sensitivity of the assay system through strain modification and mutant screening.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Luca Giannelli, Chihiro Watanabe, Hideki Yamaji, Tomohisa Katsuda
Summary: Computational fluid dynamics (CFD) was used to investigate the effect of flow regimes on the photosynthetic productivity in cascade photobioreactors (PBRs) with different bottom configurations. The study found that increasing flow rate in the flat-bottom PBR decreased areal biomass productivity due to a decrease in volumetric photon flux densities. On the other hand, the swirling flow in the wavy-bottom PBR improved photosynthetic productivity by extending the culture's stay in the illuminated lane.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Engineering, Chemical
Tomohisa Katsuda, Tomoki Yamaguchi, Yuki Kase, Kou Ota, Hideki Yamaji
Summary: Mass transfer in a protein A medium with a silica matrix was studied using confocal laser scanning microscopy. A theoretical model based on Fick's law of diffusion and Langmuir adsorption was used to approximate the mass transfer properties of IgG in single particles. The findings were validated by comparing with previous studies and reproducing the experimental breakthrough curve.
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2023)
Article
Biotechnology & Applied Microbiology
Takuya Matsuda, Toshikazu Tanijima, Akito Hirose, Kyoko Masumi-Koizumi, Tomohisa Katsuda, Hideki Yamaji
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Biotechnology & Applied Microbiology
Yuki Ohmuro-Matsuyama, Hideki Yamaji