Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors

(2018) Thomas Vogl et al.   BIOTECHNOLOGY AND BIOENGINEERING

Superior proteina titers in half the fermentation time: Promoter and process engineering for the glucose-regulated GTH1 promoter of Pichia pastoris

(2018) Roland Prielhofer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

(2017) Xiao-Wei Yu et al.   Scientific Reports

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

(2017) Elena Cámara et al.   Scientific Reports

Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris

(2017) Jinjia Wang et al.   Scientific Reports

Methanol Expression Regulator 1 (Mxr1p) Is Essential for the Utilization of Amino Acids as the Sole Source of Carbon by the Methylotrophic Yeast,Pichia pastoris

(2016) Umakant Sahu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter inPichia pastoris

(2016) Xiaolong Wang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A novel methanol-free Pichia pastoris system for recombinant protein expression

(2016) Wei Shen et al.   Microbial Cell Factories

PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris

(2015) Xiaolong Wang et al.   BIOTECHNOLOGY LETTERS

Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level

(2015) Roland Prielhofer et al.   BMC GENOMICS

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Trm1p, a Zn(II)2Cys6-type transcription factor, is essential for the transcriptional activation of genes of methanol utilization pathway, in Pichia pastoris

(2014) Umakant Sahu et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

(2014) Claudia Ruth et al.   Microbial Cell Factories

Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks

(2014) Zharain Bawa et al.   Microbial Cell Factories

Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

(2013) Shuli Liang et al.   BMC GENOMICS

Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1

(2013) Nikolai V Ravin et al.   BMC GENOMICS

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

(2013) Roland Prielhofer et al.   Microbial Cell Factories

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Overexpression and characterization of a thermostable, pH-stable and organic solvent-tolerant Ganoderma fornicatum laccase in Pichia pastoris

(2011) Wan-Ting Huang et al.   PROCESS BIOCHEMISTRY

Catabolite Repression of Aox in Pichia pastoris Is Dependent on Hexose Transporter PpHxt1 and Pexophagy

(2010) P. Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering of protein secretion in yeast: strategies and impact on protein production

(2010) Alimjan Idiris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

The Effect of Temperature on the Proteome of RecombinantPichia pastoris

(2009) Martin Dragosits et al.   JOURNAL OF PROTEOME RESEARCH

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

(2008) Brigitte Gasser et al.   Microbial Cell Factories

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH