Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

Effect of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris

(2016) Jun Yang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Droplet digital PCR-aided screening and characterization ofPichia pastorismultiple gene copy strains

(2016) Elena Cámara et al.   BIOTECHNOLOGY AND BIOENGINEERING

A step forward to improve recombinant protein production in Pichia pastoris : From specific growth rate effect on protein secretion to carbon-starving conditions as advanced strategy

(2016) Xavier Garcia-Ortega et al.   PROCESS BIOCHEMISTRY

High-level expression of Proteinase K from Tritirachium album Limber in Pichia pastoris using multi-copy expression strains

(2016) Hu Yang et al.   PROTEIN EXPRESSION AND PURIFICATION

Molecular optimization of rabies virus glycoprotein expression inPichia pastoris

(2016) Safa Ben Azoun et al.   Microbial Biotechnology

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

Systems-level organization of yeast methylotrophic lifestyle

(2015) Hannes Rußmayer et al.   BMC BIOLOGY

Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

(2015) Bryn Edwards-Jones et al.   PLoS One

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Isolation and characterization of the plasma membrane from the yeast Pichia pastoris

(2014) Karlheinz Grillitsch et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel

(2014) Jinjin Huang et al.   Biotechnology for Biofuels

Nucleocytosolic Depletion of the Energy Metabolite Acetyl-Coenzyme A Stimulates Autophagy and Prolongs Lifespan

(2014) Tobias Eisenberg et al.   Cell Metabolism

Evolving models for peroxisome biogenesis

(2014) Ewald H Hettema et al.   CURRENT OPINION IN CELL BIOLOGY

Direct Evaluation of the Effect of Gene Dosage on Secretion of Protein from Yeast Pichia pastoris by Expressing EGFP

(2014) Hailong Liu et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

(2014) Claudia Ruth et al.   Microbial Cell Factories

Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

(2013) Andrew R. Hesketh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

InPichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response

(2013) Corinna Rebnegger et al.   Biotechnology Journal

Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using deep paired-end RNA sequencing

(2013) Shuli Liang et al.   BMC GENOMICS

Process Control and Optimization for Heterologous Protein Production by Methylotrophic Pichia pastoris

(2013) Minjie GAO et al.   CHINESE JOURNAL OF CHEMICAL ENGINEERING

Enhancement of lipase r27RCL production in Pichia pastoris by regulating gene dosage and co-expression with chaperone protein disulfide isomerase

(2013) Chong Sha et al.   ENZYME AND MICROBIAL TECHNOLOGY

Atg18 phosphoregulation controls organellar dynamics by modulating its phosphoinositide-binding activity

(2013) Naoki Tamura et al.   JOURNAL OF CELL BIOLOGY

Overcoming the metabolic burden of protein secretion in Schizosaccharomyces pombe – A quantitative approach using 13C-based metabolic flux analysis

(2013) Tobias Klein et al.   METABOLIC ENGINEERING

A Defined Methodology for Reliable Quantification of Western Blot Data

(2013) Sean C. Taylor et al.   MOLECULAR BIOTECHNOLOGY

Peroxisomes take shape

(2013) Jennifer J. Smith et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived 13C-labelling data from proteinogenic amino acids

(2013) Joel Jordà et al.   New Biotechnology

Correction: Transcriptome Analysis of Medicinal Plant Salvia miltiorrhiza and Identification of Genes Related to Tanshinone Biosynthesis

(2013) Lei Yang et al.   PLoS One

Peroxisomal Fatty Acid Uptake Mechanism inSaccharomyces cerevisiae

(2012) Carlo W. T. van Roermund et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Acetyl-CoA Carboxylase Regulates Global Histone Acetylation

(2012) Luciano Galdieri et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

(2012) Joel Jordà et al.   Microbial Cell Factories

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: catabolic adaptation, stress responses, and autophagic processes

(2012) Ana Leticia Vanz et al.   Microbial Cell Factories

Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction

(2012) Pabitra K. Parua et al.   MOLECULAR MICROBIOLOGY

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes

(2010) Taicheng Zhu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A multi-level study of recombinant Pichia pastoris in different oxygen conditions

(2010) Kristin Baumann et al.   BMC Systems Biology

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

Identification of Mxr1p-binding sites in the promoters of genes encoding dihydroxyacetone synthase and peroxin 8 of the methylotrophic yeast Pichia pastoris

(2010) Balla Venkata Kranthi et al.   YEAST

ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks

(2009) Gabriela Bindea et al.   BIOINFORMATICS

Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeastPichia pastoris

(2009) T. Zhu et al.   JOURNAL OF APPLIED MICROBIOLOGY

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter

(2009) David Resina et al.   New Biotechnology

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

(2008) Alexandra Graf et al.   BMC GENOMICS