Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates

Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies

(2017) Rey W. Martin et al.   ACS Synthetic Biology

Antibodies to watch in 2017

(2016) Janice M. Reichert   mAbs

Phage display-derived human antibodies in clinical development and therapy

(2016) André Frenzel et al.   mAbs

Single Chain Antibodies as Tools to Study transforming growth factor-β-Regulated SMAD Proteins in Proximity Ligation-Based Pharmacological Screens

(2016) Andries Blokzijl et al.   MOLECULAR & CELLULAR PROTEOMICS

Protein synthesis directly from PCR: progress and applications of cell-free protein synthesis with linear DNA

(2016) Song-Min Schinn et al.   New Biotechnology

Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of “Difficult-to-Express” Proteins and Future Perspectives

(2016) Lena Thoring et al.   PLoS One

Antibodies to watch in 2016

(2015) Janice M. Reichert   mAbs

Production of Site-Specific Antibody–Drug Conjugates Using Optimized Non-Natural Amino Acids in a Cell-Free Expression System

(2014) Erik S. Zimmerman et al.   BIOCONJUGATE CHEMISTRY

Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

(2014) Marlitt Stech et al.   ENGINEERING IN LIFE SCIENCES

Engineering toward a bacterial “endoplasmic reticulum” for the rapid expression of immunoglobulin proteins

(2014) Dan Groff et al.   mAbs

A Continuous-Exchange Cell-Free Protein Synthesis System Based on Extracts from Cultured Insect Cells

(2014) Marlitt Stech et al.   PLoS One

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

The influence of antibody fragment format on phage display based affinity maturation of IgG

(2013) Miriam Steinwand et al.   mAbs

CHO cell engineering to prevent polypeptide aggregation and improve therapeutic protein secretion

(2013) Valérie Le Fourn et al.   METABOLIC ENGINEERING

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

(2013) Andreas K. Brödel et al.   PLoS One

Optimized signal peptides for the development of high expressing CHO cell lines

(2012) Lars Kober et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of functional antibody fragments in a vesicle-based eukaryotic cell-free translation system

(2012) Marlitt Stech et al.   JOURNAL OF BIOTECHNOLOGY

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient disulfide bond formation in virus-like particles

(2011) Bradley C. Bundy et al.   JOURNAL OF BIOTECHNOLOGY

Rise and Fall of an Anti-MUC1 Specific Antibody

(2011) Holger Thie et al.   PLoS One

Site-Specific Incorporation ofp-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation

(2010) Bradley C. Bundy et al.   BIOCONJUGATE CHEMISTRY

A human scFv antibody generation pipeline for proteome research

(2010) Michael Hust et al.   JOURNAL OF BIOTECHNOLOGY

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs

Rational improvement of cell-free protein synthesis

(2010) Anders Pedersen et al.   New Biotechnology

How antibodies fold

(2009) Matthias J. Feige et al.   TRENDS IN BIOCHEMICAL SCIENCES

Recombinant antibody therapeutics: the impact of glycosylation on mechanisms of action

(2009) Royston Jefferis   TRENDS IN PHARMACOLOGICAL SCIENCES

Human antibody RNase fusion protein targeting CD30+ lymphomas

(2008) C. Menzel et al.   BLOOD

Synthesis and characterization of a functional intact IgG in a prokaryotic cell-free expression system

(2007) Stephan Frey et al.   BIOLOGICAL CHEMISTRY