Article
Chemistry, Multidisciplinary
Marcel Gausmann, Christian Kocks, Johannes Pastoors, Jochen Buchs, Nick Wierckx, Andreas Jupke
Summary: This work presents a biobased production route for itaconic acid, featuring low pH value fermentation, reactive extraction, and electrochemical product recovery, resulting in a purity of more than 99% recovery of itaconic acid in crystalline form from the fermentation broth. The proposed downstream process can efficiently recover itaconic acid while eliminating the generation of waste salts, operating with electricity costs lower than sulfuric acid and sodium hydroxide typically required for pH-swing steps.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Sinah Tonjes, Evelien Uitterhaegen, Pieter De Brabander, Ellen Verhoeven, Tom Delmulle, Karel De Winter, Wim Soetaert
Summary: In this study, a fermentation strategy with in situ product recovery was developed to improve the production efficiency of muconic acid and overcome product inhibition. By integrating reactive extraction into the fermentation process, bacterial growth and muconic acid titer were increased by 44% and 18% respectively. The strategy was successfully applied in a 10 L bioreactor with a final muconic acid titer of 4.33 g L-1 and a maximum productivity of 0.053 g L-1, demonstrating its potential for enhancing challenging fermentation processes in the industrial biochemical field.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Chemical
Qiyu Meng, Jiemiao Yu, Liangrong Yang, Yanqing Li, Mo Xian, Huizhou Liu
Summary: Effective isolation of 1,2,4-butanetriol from fermentation broth with low concentrations was achieved through anionic reactive extraction using a boronic acid carrier and A336 dissolved in the organic phase. The study explored the impact of various factors on extraction efficiency and successfully concentrated 1,2,4-butanetriol by 4.6 times compared to the original fermentation broth.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Engineering, Chemical
Moritz Doeker, Vincent Huettche, Andreas Jupke
Summary: Biotechnological transformations offer an efficient alternative for producing amines and amino alcohols, but face limitations due to amine toxicity and reaction equilibrium. This study explores liquid-liquid reactive extraction for recovering amines from aqueous streams, achieving high extraction yields and providing a mathematical tool for designing tailor-made extraction systems while meeting biotechnological pH requirements.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Patchara Chaichol, Nopphon Weeranoppanant
Summary: Liquid-liquid extraction (LLE) is commonly used for downstream purification in bioprocesses. The in situ mode performs LLE simultaneously with fermentation inside the fermenter, while the in-line mode separates LLE in an external extraction unit. Both modes intensify the bioprocess and enhance productivity by removing inhibitory species continuously. This review provides an overview of applications and highlights the roles of these two modes in bioprocess intensification. Advances in equipment and process designs, along with key examples, are also discussed.
REACTION CHEMISTRY & ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Pieter De Brabander, Evelien Uitterhaegen, Ellen Verhoeven, Cedric Vander Cruyssen, Karel De Winter, Wim Soetaert
Summary: The study identified biocompatible solvents and solvent mixtures for the extraction of industrial platform chemicals, enhancing the efficiency of extractive fermentations.
FERMENTATION-BASEL
(2021)
Article
Chemistry, Physical
Lea Nolte, Christoph Brandenbusch
Summary: The downstream processing of biocatalytic (di-)carboxylic acids is challenging, but an innovative approach that utilizes hydrophobic and water soluble amine extractants for reactive extraction and back-extraction demonstrates industrial applicability. A deeper understanding of the extraction processes on a molecular level is necessary for optimized process design and downstream processing.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biotechnology & Applied Microbiology
Shourabh Singh Raghuwanshi, Kailas L. Wasewar, Kanti Kumar Athankar
Summary: This study focused on extracting itaconic acid using reactive tri-n-butyl phosphate dissolved in natural, non-toxic diluents like sunflower oil, rice bran oil, and sesame oil. The results showed that sunflower oil had the highest extraction efficiency and distribution coefficient for itaconic acid.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Review
Agricultural Engineering
Priskila Adjani Diankristanti, I-Son Ng
Summary: Microbial biomanufacturing is a promising approach for the production of high-value compounds with minimal environmental impact and economic benefits. Itaconic acid (IA) is an important platform chemical with various applications, and efforts have been made to engineer non-native hosts for its production. This review provides an update on the progress made in IA bioproduction, from native to engineered hosts, and discusses both in vivo and in vitro approaches, as well as potential combination strategies. The challenges and recent endeavors in renewable IA production are also addressed for sustainable development goals (SDGs).
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Chemical
Tamas Rozsenberszki, Peter Komaromy, Eva Hulber-Beyer, Peter Bakonyi, Nandor Nemestothy, Katalin Belafi-Bako
Summary: This study assessed the feasibility of IA separation from fermentation broths produced by Aspergillus terreus using commercialized electrodialysis unit with mono-and bipolar membranes. Experimental results showed that higher initial pH resulted in faster IA separation but decreased current efficiency, with by-products affecting separation efficiency.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2021)
Article
Chemistry, Physical
Lea Nolte, Mike Nowaczyk, Christoph Brandenbusch
Summary: This study successfully applied Fourier Transform Infrared (FTIR) spectroscopy to detect and quantify the acid-extractant complex formed between itaconic acid (IA) and tri-noctylamine (TNOA) in 1-decanol, and the experimental setup enabled online monitoring of the complex concentration and interaction mechanism.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Agricultural Engineering
Samarpita Roy, Opeyemi Olokede, Haoran Wu, Mark Holtzapple
Summary: By utilizing methane-arrested mixed cultures, organic substrates were fermented to mixed carboxylic acids at higher yields, and the product rate was increased through the use of ion-exchange resins and other methods. The results suggest that further improvements in the MixAlco (R) process configurations have great potential.
BIOMASS & BIOENERGY
(2021)
Article
Biology
Nur Imanina Abdullah Thaidi, Rosfarizan Mohamad, Helmi Wasoh, Mohammad Rizal Kapri, Ahmad Badruddin Ghazali, Joo Shun Tan, Leonardo Rios-Solis, Murni Halim
Summary: High broth viscosity due to HA accumulation limits HA yield in Streptococcus zooepidemicus production. Extractive fermentation via in situ product recovery (ISPR) was used to enhance HA production. The resin IRA67 showed high adsorption capacity for HA. The cultivation with an internal column using IRA67 resin resulted in 1.36-fold improvement in HA production and significant reduction in viscosity, indicating the effectiveness of ISPR adsorption.
Article
Green & Sustainable Science & Technology
Wenyan Zhao, Veeriah Jegatheesan, Qiaochu Liang, Khantong Soontarapa, Heqing Jiang, Yang Zhang, Binghua Yan
Summary: The study integrated a tailored electrodialysis stack with a fermenter to improve the recovery of carboxylic acids from organic wastes and achieve minimum carbon footprint. Experimental results showed that electrodialysis process helped increase carboxylic acids yield and impact the stability of microbial community.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Multidisciplinary
Patrick O. Saboe, Lorenz P. Manker, Hanna R. Monroe, William E. Michener, Stefan Haugen, Eric C. D. Tan, Ryan L. Prestangen, Gregg T. Beckham, Eric M. Karp
Summary: Bio-based carboxylic acids are established as adaptable precursors to renewable biofuels and chemicals, but their separation poses significant energy and cost challenges. The use of weak-base adsorption processes shows promising results in reducing operating costs, energy consumption, and greenhouse gas emissions compared to conventional methods.
Article
Food Science & Technology
Neha Babbar, Winnie Dejonghe, Stefano Sforza, Kathy Elst
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2017)
Article
Chemistry, Applied
Stefania Baldassarre, Neha Babbar, Sandra Van Roy, Winnie Dejonghe, Miranda Maesen, Stefano Sforza, Kathy Elst
Article
Biochemistry & Molecular Biology
Barbara Prandi, Stefania Baldassarre, Neha Babbar, Elena Bancalari, Pieter Vandezande, David Hermans, Geert Bruggeman, Monica Gatti, Kathy Elst, Stefano Sforza
Article
Chemistry, Multidisciplinary
Guneet Kaur, Miranda Maesen, Linsey Garcia-Gonzalez, Heleen De Weyer, Kathy Elst
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2018)
Article
Biochemistry & Molecular Biology
Kathy Elst, Miranda Maesen, Griet Jacobs, Leen Bastiaens, Stefan Voorspoels, Kelly Servaes
Article
Biotechnology & Applied Microbiology
Emre Gunerken, Els D'Honde, Michel H. M. Eppink, Rene H. Wijffels, Kathy Elst
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2019)
Article
Chemistry, Applied
Barbara Prandi, Andrea Faccini, Francesca Lambertini, Mariangela Bencivenni, Montse Jorba, Bart Van Droogenbroek, Geert Bruggeman, Juergen Schoeber, Janos Petrusan, Kathy Elst, Stefano Sforza
Article
Engineering, Chemical
Alexandra Bouriakova, Pedro S. F. Mendes, Kathy Elst, Jeriffa De Clercq, Joris W. Thybaut
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2020)
Review
Food Science & Technology
Jose Villacis-Chiriboga, Kathy Elst, John Van Camp, Edwin Vera, Jenny Ruales
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2020)
Article
Biotechnology & Applied Microbiology
Guneet Kaur, Linsey Garcia-Gonzalez, Kathy Elst, Federica Truzzi, Lorenzo Bertin, Ankita Kaushik, Malini Balakrishnan, Heleen De Wever
BIOCHEMICAL ENGINEERING JOURNAL
(2020)
Article
Biochemistry & Molecular Biology
Sandra Corderi, Tom Renders, Kelly Servaes, Karolien Vanbroekhoven, Tony De Roo, Kathy Elst
Summary: The study presents two simple methods for removing polysaccharides from biorefinery lignin samples, which can reduce the polysaccharide content of lignin and have economic potential for the production of bio-based chemicals and materials.
Article
Food Science & Technology
Jose Villacis-Chiriboga, Stefan Voorspoels, Maarten Uyttebroek, Jenny Ruales, John Van Camp, Edwin Vera, Kathy Elst
Summary: The potential of supercritical CO2 for extracting bioactive compounds from mango by-products was evaluated using a design of experiments. Optimal conditions were found for carotenoid extraction, with the peel fraction showing significantly higher bioactive content compared to the pulp fraction. Supercritical CO2 is a promising technology for isolating valuable compounds from mango by-products.
Article
Engineering, Environmental
Deepak Raikwar, Korneel Van Aelst, Thijs Vangeel, Sandra Corderi, Joost Van Aelst, Sander Van den Bosch, Kelly Servaes, Karolien Vanbroekhoven, Kathy Elst, Bert F. Sels
Summary: Unsatisfactory solubility and recalcitrant condensed structures of isolated lignins (technical lignins) severely limit their processability. Understanding the structural and physicochemical properties of isolated lignins is crucial for explaining their performance in solubilization and further depolymerization. This study investigated the solubilization and depolymerization of organosolv lignins from three different sources. Solubility tests were conducted in six organic solvents, and solubilization behavior was described based on structural properties and investigated under different conditions. Strong structural correlations between parent lignin/subfractions and depolymerized lignin oils were derived.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Food Science & Technology
Jose Villacis-Chiriboga, Barbara Prandi, Jenny Ruales, John Van Camp, Stefano Sforza, Kathy Elst
Summary: This research investigated the valorization of soursop seeds to produce oil and proteins. A new mild protein extraction method (Pressurized Water Extraction, PWE) was developed and optimized, and compared with conventional alkaline extraction. The impact of the de-oiling technique was also investigated. The extracted proteins were evaluated in terms of physicochemical composition, amino acid content, and techno-functional properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Kathy Elst, Neha Babbar, Sandra Van Roy, Stefania Baldassarre, Winnie Dejonghe, Miranda Maesen, Stefano Sforza
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2018)