Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase

Improving Cell-Free Protein Synthesis through Genome Engineering ofEscherichia coliLacking Release Factor 1

(2015) Seok Hoon Hong et al.   CHEMBIOCHEM

Energizing eukaryotic cell-free protein synthesis with glucose metabolism

(2015) Mark J. Anderson et al.   FEBS LETTERS

Creating small transcription activating RNAs

(2015) James Chappell et al.   Nature Chemical Biology

pH responsive granulocyte colony-stimulating factor variants with implications for treating Alzheimer's disease and other central nervous system disorders

(2015) Pete Heinzelman et al.   PROTEIN ENGINEERING DESIGN & SELECTION

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

(2015) Yong-Chan Kwon et al.   Scientific Reports

Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

(2014) Robert B. Quast et al.   ANALYTICAL BIOCHEMISTRY

Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis

(2014) Alaksh Choudhury et al.   BIOCHEMICAL ENGINEERING JOURNAL

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

A combined cell-free transcription-translation system fromSaccharomyces cerevisiaefor rapid and robust protein synthe

(2014) Rui Gan et al.   Biotechnology Journal

Paper-Based Synthetic Gene Networks

(2014) Keith Pardee et al.   CELL

Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes

(2014) Rita Sachse et al.   FEBS LETTERS

Quantifying Position-Dependent Codon Usage Bias

(2014) Adam J. Hockenberry et al.   MOLECULAR BIOLOGY AND EVOLUTION

Characterizing IGR IRES-mediated translation initiation for use in yeast cell-free protein synthesis

(2014) C. Eric Hodgman et al.   New Biotechnology

In vitro Fab display: a cell-free system for IgG discovery

(2014) R. L. Stafford et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The HydG Enzyme Generates an Fe(CO)2(CN) Synthon in Assembly of the FeFe Hydrogenase H-Cluster

(2014) J. M. Kuchenreuther et al.   SCIENCE

Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard”

(2014) Dan Siegal-Gaskins et al.   ACS Synthetic Biology

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis

(2014) Seok Hoon Hong et al.   Frontiers in Chemistry

Molecular Cloning and Heterologous Expression of Laccase from Aeromonas hydrophila NIU01 in Escherichia coli with Parameters Optimization in Production

(2013) I-Son Ng et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

(2013) Jennifer A. Schoborg et al.   Biotechnology Journal

Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation

(2013) Cem Albayrak et al.   NUCLEIC ACIDS RESEARCH

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology

(2013) James Chappell et al.   NUCLEIC ACIDS RESEARCH

A Radical Intermediate in Tyrosine Scission to the CO and CN- Ligands of FeFe Hydrogenase

(2013) J. M. Kuchenreuther et al.   SCIENCE

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System

(2013) Zachary Z. Sun et al.   ACS Synthetic Biology

Integrating cell-free biosyntheses of heme prosthetic group and apoenzyme for the synthesis of functional P450 monooxygenase

(2012) Yong-Chan Kwon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

New Insights into [FeFe] Hydrogenase Activation and Maturase Function

(2012) Jon M. Kuchenreuther et al.   PLoS One

Transforming biochemical engineering with cell-free biology

(2011) James R. Swartz   AICHE JOURNAL

High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery

(2011) Tae-Wan Kim et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins

(2011) Cassandra Guarino et al.   GLYCOBIOLOGY

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

Cell-free H-cluster Synthesis and [FeFe] Hydrogenase Activation: All Five CO and CN− Ligands Derive from Tyrosine

(2011) Jon M. Kuchenreuther et al.   PLoS One

Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunitafold similar to the protein in the cell membrane

(2011) Eva-Maria E. Uhlemann et al.   PROTEIN SCIENCE

Expression of industrially relevant laccases: prokaryotic style

(2011) Navaneetha Santhanam et al.   TRENDS IN BIOTECHNOLOGY

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Cloning-Independent Expression and Analysis of  -Transaminases by Use of a Cell-Free Protein Synthesis System

(2010) Y.-C. Kwon et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Site-Specific Incorporation ofp-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation

(2010) Bradley C. Bundy et al.   BIOCONJUGATE CHEMISTRY

Molecular determinants of peculiar properties of a Pleurotus ostreatus laccase: Analysis by site-directed mutagenesis

(2009) Flavia Autore et al.   ENZYME AND MICROBIAL TECHNOLOGY

Crystal Structure of a Two-domain Multicopper Oxidase

(2009) Thomas J. Lawton et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Homologous cloning, expression, and characterisation of a laccase from Streptomyces coelicolor and enzymatic decolourisation of an indigo dye

(2008) Etienne Dubé et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology

Human protein factory for converting the transcriptome into an in vitro–expressed proteome

(2008) Naoki Goshima et al.   NATURE METHODS

Cell-free synthesis and maturation of [FeFe] hydrogenases

(2007) Marcus E. Boyer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Rapid Expression and Purification of 100 nmol Quantities of Active Protein Using Cell-Free Protein Synthesis

(2004) Michael C. Jewett et al.   BIOTECHNOLOGY PROGRESS