Energizing eukaryotic cell-free protein synthesis with glucose metabolism

Improving Cell-Free Protein Synthesis through Genome Engineering ofEscherichia coliLacking Release Factor 1

(2015) Seok Hoon Hong et al.   CHEMBIOCHEM

A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system

(2015) Filippo Caschera et al.   METABOLIC ENGINEERING

Creating small transcription activating RNAs

(2015) James Chappell et al.   Nature Chemical Biology

Implications of macromolecular crowding and reducing conditions for in vitro ribosome construction

(2015) Brian R. Fritz et al.   NUCLEIC ACIDS RESEARCH

Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis

(2014) Alaksh Choudhury et al.   BIOCHEMICAL ENGINEERING JOURNAL

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system

(2014) Filippo Caschera et al.   BIOCHIMIE

A combined cell-free transcription-translation system fromSaccharomyces cerevisiaefor rapid and robust protein synthe

(2014) Rui Gan et al.   Biotechnology Journal

The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction

(2014) Brian R. Fritz et al.   NUCLEIC ACIDS RESEARCH

Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures

(2014) Waleed Ahmad Khattak et al.   PROCESS BIOCHEMISTRY

Optimized extract preparation methods and reaction conditions for improved yeast cell-free protein synthesis

(2013) C. Eric Hodgman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

(2013) Jennifer A. Schoborg et al.   Biotechnology Journal

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation

(2013) M. C. Jewett et al.   Molecular Systems Biology

IRES-Mediated Translation of Membrane Proteins and Glycoproteins in Eukaryotic Cell-Free Systems

(2013) Andreas K. Brödel et al.   PLoS One

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System

(2013) Zachary Z. Sun et al.   ACS Synthetic Biology

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

(2012) Gang Yin et al.   mAbs

Cell-free protein synthesis: Applications come of age

(2011) Erik D. Carlson et al.   BIOTECHNOLOGY ADVANCES

Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines

(2011) James F. Zawada et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell-free synthetic biology: Thinking outside the cell

(2011) C. Eric Hodgman et al.   METABOLIC ENGINEERING

Expression optimization and synthetic gene networks in cell-free systems

(2011) David K. Karig et al.   NUCLEIC ACIDS RESEARCH

Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source

(2011) Ho-Cheol Kim et al.   PROCESS BIOCHEMISTRY

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

(2011) Jonghyeon Shin et al.   ACS Synthetic Biology

Practical cell-free protein synthesis system using purified wheat embryos

(2010) Kazuyuki Takai et al.   Nature Protocols

Cell-free protein synthesis energized by slowly-metabolized maltodextrin

(2009) Yiran Wang et al.   BMC BIOTECHNOLOGY

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology

An Economical Method for Cell-Free Protein Synthesis using Glucose and Nucleoside Monophosphates

(2005) Kara A. Calhoun et al.   BIOTECHNOLOGY PROGRESS