Article
Multidisciplinary Sciences
Marc Bathe-Peters, Philipp Gmach, Horst-Holger Boltz, Jurgen Einsiedel, Michael Gotthardt, Harald Huebner, Peter Gmeiner, Martin J. Lohse, Paolo Annibale
Summary: Two beta-adrenergic receptor subtypes, beta(1) and beta(2), show different localizations and diffusions in cardiomyocytes, with the segregation depending on the development of the T-tubular network, leading to distinct signaling and functional effects.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Xin Shen, Jonas van den Brink, Anna Bergan-Dahl, Terje R. Kolstad, Einar S. Norden, Yufeng Hou, Martin Laasmaa, Yuriana Aguilar-Sanchez, Ann P. Quick, Emil K. S. Espe, Ivar Sjaastad, Xander H. T. Wehrens, Andrew G. Edwards, Christian Soeller, William E. Louch
Summary: This study investigates the promotion of RyR cluster dispersion by prolonged beta-adrenergic stimulation in heart failure, and examines the underlying mechanisms. The findings demonstrate the intimate connection between RyR localization and function through channel phosphorylation by CaMKII and PKA, which plays a protective role in healthy cardiomyocytes but contributes to impaired cardiac function during heart failure.
Article
Biochemistry & Molecular Biology
Ingrid Matzer, Julia Voglhuber, Mara Kiessling, Natasa Djalinac, Viktoria Trummer-Herbst, Nishani Mabotuwana, Lavinia Rech, Michael Holzer, Samuel Sossalla, Peter P. Rainer, Andreas Zirlik, Senka Ljubojevic-Holzer
Summary: This study investigates the effects of excessive beta-adrenergic stimulation and tachycardia on gene expression profiles of cardiomyocytes. The results show that tachycardia leads to upregulation of sodium-calcium exchanger (NCX) and activation of calcium/calmodulin-dependent kinase II (CaMKII). Isoprenaline treatment ameliorates NCX upregulation and potentiate CaMKII activity, while propranolol abolishes these effects. Additionally, tachycardia induces upregulation of interleukin-6 receptor (IL6R), which can be prevented by isoprenaline and the inhibition of NCX.
Editorial Material
Cell Biology
Liliana Felicia Iannucci, Giulietta Di Benedetto, Konstantinos Lefkimmiatis
Summary: Macroautophagy/autophagy is a cellular process responsible for eliminating and recycling aggregated proteins and damaged organelles. The compartmentalization of PRKA/PKA determines its effects on autophagy, with increased cAMP levels generating different PRKA activity signatures. The distribution of PRKA holoenzymes plays a role in affecting the autophagic flux in specific cell types.
Article
Biochemistry & Molecular Biology
Philipp Gmach, Marc Bathe-Peters, Narasimha Telugu, Duncan C. Miller, Paolo Annibale
Summary: The potential of human-induced pluripotent stem cells (hiPSCs) to differentiate into cardiomyocytes (CMs) has been studied, but the evolution of CM-specific receptor proteins during differentiation has received limited attention. This study uses fluorescent spectroscopy to observe the changes in plasma membrane abundance of beta(1)- and beta(2)-adrenergic receptors in hiPSC-derived CMs during long-term culture. The observations are compared to mRNA levels in wildtype hiPSCs and CRISPR/Cas9 knock-in clones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cardiac & Cardiovascular Systems
Peyton B. Sandroni, Kelsey H. Fisher-Wellman, Brian C. Jensen
Summary: This article reviews recent studies demonstrating the differential regulation of cardiac contractility by alpha 1- and beta-ARs in cardiomyocytes, with evidence suggesting that alpha 1-ARs may mitigate the deleterious effects of chronic beta-AR activation.
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jinhee H. Hwang, Michael E. Spurlock, John C. Kube, Xiang Z. Li, Stephen B. Smith
Summary: The study revealed that lubabegron fumarate is highly selective for the beta(3)-adrenergic receptor subtype and has minimal agonistic activity at the beta(2)-adrenergic receptor. Differences in mRNA expression levels of beta(1)-, beta(2)-, and beta(3)-AR were observed between subcutaneous and intramuscular adipose tissues. The response to beta-adrenergic ligands, especially agonists for the beta(1)- and beta(3)-receptor subtypes, was found to be lower in intramuscular adipose tissue compared to subcutaneous adipose tissue.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Meng-Meng Du, Zhan-Tao Zhu, Ge-Ge Zhang, Yun-Qiu Zhao, Bei Gao, Xin-Yi Tao, Min Liu, Yu-Hong Ren, Feng-Qing Wang, Dong-Zhi Wei
Summary: This study aimed to enhance beta-amyrin production in Saccharomyces cerevisiae by compartmentalizing the enhanced squalene synthesis pathway into peroxisomes. Through this approach, combined with the push-pull-restrain strategy, the production of beta-amyrin was significantly increased, reaching the highest reported titer to date. The study provides a new perspective on engineering yeasts for triterpene production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Meng-Meng Du, Zhan-Tao Zhu, Ge-Ge Zhang, Yun-Qiu Zhao, Bei Gao, Xin-Yi Tao, Min Liu, Yu-Hong Ren, Feng-Qing Wang, Dong-Zhi Wei
Summary: The study aims to enhance the production of beta-amyrin in Saccharomyces cerevisiae through peroxisome compartmentalization and the push-pull-restrain strategy. The results show that peroxisome compartmentalization can mitigate the inhibitory effect of overaccumulated squalene on beta-amyrin synthase activity and significantly increase its production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Jinhee H. Hwang, John C. Kube, Stephen B. Smith
Summary: The study demonstrated that lubabegron fumarate acts as an antagonist to beta-adrenergic receptor subtypes in subcutaneous adipocytes and exhibits different responses to beta-AA and LUB in subcutaneous and intramuscular adipocytes.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
Shijie Liu, Li Tang, Xiaolei Zhao, Bao Nguyen, Todd R. Heallen, Min Li, Jianxin Wang, Jun Wang, James F. Martin
Summary: The study found that Wntless (Wls) is a direct target gene of Yap during neonatal heart regeneration, involved in communication between cardiomyocytes and cardiac fibroblasts. Deletion of Wls in neonatal cardiomyocytes disrupts Wnt signaling pathway, which impairs heart regeneration process.
CIRCULATION RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Kimberly J. McCabe, Padmini Rangamani
Summary: The cAMP/PKA pathway plays a crucial role in regulating excitation-contraction coupling in cardiomyocytes, with downstream effects on cardiac function. Experimental advances have revealed compartmentation of cAMP and PKA, while computational modeling helps to understand the spatial and temporal complexities of this system. Various compartmental and spatial signaling models have been used to study adrenergic signaling pathways in different simulation conditions, with newer subcellular models of cardiovascular function being explored for future study.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lorenzo Fassina, Maria Rita Assenza, Michele Miragoli, Andrea M. Isidori, Fabio Naro, Federica Barbagallo
Summary: Intracellular calcium plays a crucial role in heart contractility and its alterations can lead to cardiac dysfunction. Simultaneous analysis of contractile function and intracellular calcium is necessary to evaluate cardiomyocyte activity. This study presents a user-friendly image processing and analysis tool based on ImageJ-Fiji/MATLAB (R) software to assess major cardiomyocyte functional parameters. The method successfully analyzed fractional cell shortening, calcium transient amplitude, and kinematics/dynamics parameters in isolated adult mouse cardiomyocytes.
Article
Cell Biology
Jin-Sook Kwon, Eric W. Barr, J. Kurt Chuprun, Walter J. Koch
Summary: Stimulation of alpha- or beta-adrenergic receptors in mice led to changes in the content of circulating blood EVs, with differential expression patterns of miRs. Pathway analysis revealed common and distinct pathways affected by PE and ISO treatment, indicating regulation of specific pathways by alpha- or beta-AR agonists. This study suggests that chronic sympathetic nervous system activation in heart failure may alter the content of EVs secreted from the heart and other organs, potentially impacting pathological remodeling.
Article
Medicine, Research & Experimental
Min Cheng, Can Chen, Kunwu Yu, Xiao Lv, Qiutang Zeng, Nianguo Dong, Feng Zhu
Summary: CXCR4 activation in cardiomyocytes promotes cardiomyocyte survival and heart function during beta-adrenergic stress, while the lack of CXCR4 leads to worsened cardiac function, increased cardiomyocyte apoptosis, and interstitial fibrosis. Treatment with SDF-1 attenuates the apoptotic effects of beta-adrenergic stress by promoting Akt phosphorylation, and this effect is dependent on CXCR4 and Gi signaling.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Rui Xu, Raghavender Reddy Gopireddy, Yudi Wu, Lei Wu, Xiang Tao, Ji Shao, Wenxin Wang, Li Li, Aleksandra Jovanovic, Bing Xu, Nicolas J. Kenyon, Quan Lu, Yang K. Xiang, Qin Fu
Article
Cardiac & Cardiovascular Systems
Polina Gross, Jaslyn Johnson, Carlos M. Romero, Deborah M. Eaton, Claire Poulet, Jose Sanchez-Alonso, Carla Lucarelli, Jean Ross, Andrew A. Gibb, Joanne F. Garbincius, Jonathan Lambert, Erdem Varol, Yijun Yang, Markus Wallner, Eric A. Feldsott, Hajime Kubo, Remus M. Berretta, Daohai Yu, Victor Rizzo, John Elrod, Abdelkarim Sabri, Julia Gorelik, Xiongwen Chen, Steven R. Houser
Summary: The joining region in JPH2 interacts with LTCC-alpha 1C subunit, causing LTCCs to distribute to dyads and colocalize with RyRs, facilitating dyad assembly and maintaining normal CICR in cardiomyocytes. Disruption of this interaction leads to T-tubule remodeling, impaired excitation-contraction coupling, and altered myocyte bioenergetics.
CIRCULATION RESEARCH
(2021)
Review
Physiology
Chong Liu, Ping Ke, Jingjing Zhang, Xiaoying Zhang, Xiongwen Chen
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Medicine, Research & Experimental
Lixiao Zhen, Qian Zhao, Jinhui Lu, Shengqiong Deng, Zhen Xu, Lin Zhang, Yuzhen Zhang, Huimin Fan, Xiongwen Chen, Zhongmin Liu, Yuying Gu, Zuoren Yu
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Article
Pharmacology & Pharmacy
Jing Wang, Tianshu Liu, Xiongwen Chen, Qiaofeng Jin, Yihan Chen, Li Zhang, Zhengyang Han, Dandan Chen, Yuman Li, Qing Lv, Mingxing Xie
Summary: Th17 cells play a significant role in the pathogenesis of myocarditis, and Bazedoxifene can affect STAT3 signaling to effectively inhibit the progression of cardiac inflammation. Therefore, Bazedoxifene may be a promising therapeutic strategy for the treatment of experimental autoimmune myocarditis.
FRONTIERS IN PHARMACOLOGY
(2021)
Editorial Material
Pharmacology & Pharmacy
Ya Liu, Xiongwen Chen, Hai-Gang Zhang
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xiang Tao, Haiqing He, Jiangtong Peng, Rui Xu, Jing Fu, Yuting Hu, Li Li, Xiaoyan Yang, Xiuling Feng, Chao Zhang, Lingmin Zhang, Xiyong Yu, Ao Shen, Kai Huang, Qin Fu
Summary: Recent studies have shown that PDE4-cAMP signaling is relevant to metabolic disorders. PDE4 plays a crucial role in the pathogenesis of NAFLD and associated hypertension, with PDE4 inhibitor roflumilast having the potential for future use in prevention or treatment of these conditions.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
Rui Xu, Jing Fu, Yuting Hu, Xiaoyan Yang, Xiang Tao, Long Chen, Kai Huang, Qin Fu
Summary: Patients with type 2 diabetes have a high risk of developing cardiovascular disease. Dysregulation of phosphodiesterase 4 (PDE4) is of pathophysiological importance in metabolic disorders. Inhibition of PDE4 can reverse diabetic cardiac dysfunction caused by a high-fat diet, suggesting a therapeutic potential in preventing or treating cardiac dysfunction in diabetes.
Article
Oncology
Huili Ren, Yingxiang Chen, Zhou Ao, Qi Cheng, Xiaoyan Yang, Hua Tao, Lixin Zhao, Ao Shen, Peiyuan Li, Qin Fu
Summary: This study reveals that the expression of phosphodiesterase 4D (PDE4D) is positively correlated with poor survival in hepatocellular carcinoma (HCC) patients. PDE4D binds to and interacts with Yes-associated protein (YAP) to promote HCC progression. Targeting the PDE4D-YAP interaction may be an effective strategy for the treatment of HCC.
Article
Cardiac & Cardiovascular Systems
Yijia Li, Xiongwen Chen, Yue Qi, Yichen Qu, Akshay Kumar, Songbo Dong, Yan Yang, Qiong Zhao
Summary: This study evaluated the impact of gender on Sievers types, valvulopathy, aortopathy, and outcomes of aortic valve replacement (AVR) in Chinese patients with bicuspid aortic valve (BAV). The results showed that male patients had more severe valvulopathy and aortopathy, as well as a higher risk of left ventricular dysfunction after AVR. Importantly, male patients developed these conditions at a younger age.
ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES
(2022)
Article
Cell Biology
Xiang Tao, Can Chen, Yingxiang Chen, Luoying Zhang, Jiong Hu, Hongjun Yu, Minglu Liang, Qin Fu, Kai Huang
Summary: This study reveals that beta(2)-adrenergic receptor (beta(2)AR) plays a critical role in liver regeneration by interacting with c-met and activating ERK signaling, leading to increased hepatocyte proliferation.
CELL DEATH & DISEASE
(2022)
Article
Pharmacology & Pharmacy
Rui Xu, Shi Feng, Zhou Ao, Yingxiang Chen, Congping Su, Xiuling Feng, Qin Fu, Xiaoyan Yang
Summary: Psoriasis is a chronic inflammatory disease that affects a significant portion of the population worldwide. Salmeterol, a long-acting beta(2)-AR agonist, alleviates the severity of psoriasis by inhibiting the proliferation and promoting apoptosis of keratinocytes.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Jing Fu, Li Li, Long Chen, Congping Su, Xiuling Feng, Kai Huang, Laxi Zhang, Xiaoyan Yang, Qin Fu
Summary: Inflammation plays a crucial role in the development of heart failure, with PGE2 acting as a key mediator in the inflammatory process of heart disease. PGE2 reduces the synthesis of TGF-I31 signaling pathway by inhibiting the I3-AR activated pathway, which may be a potential therapeutic target for treating heart failure.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Yijia Li, Jaslyn P. Johnson, Yijun Yang, Daohai Yu, Hajime Kubo, Remus M. Berretta, Tao Wang, Xiaoying Zhang, Michael Foster, Jun Yu, Douglas G. Tilley, Steven R. Houser, Xiongwen Chen
Summary: Maternal hypothyroidism can negatively impact the cardiac disease responses of offspring by reducing early postnatal cardiomyocyte proliferation and resulting in a smaller number of larger cardiac myocytes. These changes are associated with more severe cardiac disease responses following injury.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xin Zhang, Yijia Li, Xiaoying Zhang, Valentino Piacentino, David M. Harris, Remus Berretta, Kenneth B. Margulies, Steven R. Houser, Xiongwen Chen
