Article
Biochemistry & Molecular Biology
Tianyang Lan, Kang Zhang, Feifei Lin, Qifu He, Shenghui Wu, Zhiming Xu, Yong Zhang, Fusheng Quan
Summary: MICU1 modulators enhance mitochondrial activity, pyruvate metabolism, and developmental competence of thawed oocytes after vitrification. Vitrification leads to adverse effects on mitochondrial function, but these effects can be mitigated by MICU1 activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Keiko Tanaka, Yohei Hayashi, Asuka Takehara, Yumi Ito-Matsuoka, Masahito Tachibana, Nobuo Yaegashi, Yasuhisa Matsui
Summary: Inhibition of pyruvate uptake into mitochondria in fetal ovarian germ cells results in repressed early folliculogenesis, which may be a possible cause of defective folliculogenesis in humans. The abnormal pyruvate metabolism leads to insufficient Gdf9 expression, impacting early follicular dysgenesis.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Xiuying Jiang, Xiangning Xu, Bicheng Wang, Ke Song, Jiaqi Zhang, Ye Chen, Ying Tian, Jing Weng, Yuanjing Liang, Wei Ma
Summary: 2-Methoxyestradiol (2-ME2), a metabolite of 17 beta-estradiol, is being studied as an antitumor agent. This study found that while 2-ME2 levels remain stable in the ovaries of aging mice, it declines in the serum. Exposure to 2-ME2 affects the meiotic maturation of mouse oocytes, leading to abnormal spindle structure and chromosome alignment. The study suggests that the impairment of oocyte maturation caused by 2-ME2 exposure is due to mitochondrial imbalance, oxidative stress, and apoptosis.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Cell Biology
Xiao-Long Mo, Feng Liu, Chun-Hua Xing, Meng-Meng Shan, Bo Yao, Qi-Qi Sun, Yuan-Jing Zou, Kun-Huan Zhang, Jun Tan, Shao-Chen Sun, Yan-Ping Ren
Summary: The protein kinase PLK1 is found to be modified by SUMOylation in mouse oocytes, playing a crucial role in regulating normal meiosis. The expression of PLK1 increased and the expression of SUMO-1 and SUMO-2/3 decreased in aged mouse oocytes, suggesting a potential relationship between PLK1 SUMOylation and aging in mice.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Hyuk-Joon Jeon, Minsung Kang, Jae-Sung Kim, Jeong Su Oh
Summary: The study found that telomeres in oocytes experience shortening during postovulatory aging, but overexpression of Translationally Controlled Tumor Protein (TCTP) can reverse this shortening by upregulating telomerase activity. TCTP prevents telomere attrition during postovulatory aging in mouse oocytes by increasing telomerase activity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Obstetrics & Gynecology
Lu Liu, Jie Huang, Anlan He, Shuai Zhou, Qianneng Lu, Xiaoning Yu, Xue Bai, Mingrui Li, Ran Huo
Summary: This study found that 4,4'-dimethoxychalcone (DMC) can improve the quality of postovulatory ageing (POA) oocytes by eliminating accumulated reactive oxygen species (ROS), reducing apoptosis, improving mitochondrial distribution, and restoring the actin cap formation. DMC supplementation activates autophagy to protect oocytes from the effects of POA, leading to improved fertilization and developmental potential.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Cell Biology
Xue Yue, Sai-Li Liu, Jia-Ni Guo, Tie-Gang Meng, Xin-Ran Zhang, Hong-Xia Li, Chun-Ying Song, Zhen-Bo Wang, Heide Schatten, Qing-Yuan Sun, Xing-Ping Guo
Summary: Epitalon can delay the in vitro aging process of oocytes by reducing intracellular reactive oxygen species, decreasing spindle defects, and increasing mitochondrial activity.
Article
Cell Biology
Mingrui Li, Chao Ren, Shuai Zhou, Yuanlin He, Yueshuai Guo, Hao Zhang, Lu Liu, Qiqi Cao, Congjing Wang, Jie Huang, Yue Hu, Xue Bai, Xuejiang Guo, Wenjie Shu, Ran Huo
Summary: The study found that abnormal expression of splicing regulation proteins in aged oocytes affects the splicing of DNA repair-related genes in 2-cell embryos, reducing their developmental potential.
Article
Cell Biology
Ramya Potabattula, Tom Trapphoff, Marcus Dittrich, Kinga Fic, Grazyna E. Ptak, Stefan Dieterle, Thomas Haaf
Summary: An age-dependent increase in rDNA methylation was observed in oocytes from women with male infertility undergoing fertility treatment. The methylation levels of rDNA core promoter and UCE increased with age in both human and mouse oocytes. Variability in rDNA methylation levels suggests different epigenetic ages within oocytes from the same woman.
Article
Veterinary Sciences
Shimaa Rakha, Mohammed A. Elmetwally, Hossam El-Sheikh Ali, Ahmed Zaky Balboula, Abdelmonem Montaser Mahmoud, Samy M. Zaabel
Summary: This study found that adding lycopene to in vitro aged mouse oocytes can reduce oxidative stress damage and apoptosis, thereby delaying the postovulatory aging process. It suggests that lycopene may serve as a natural supplement to improve the success rate of assisted reproductive technologies.
VETERINARY SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jiadan Qu, Yunyao Luo, Lifeng Qin, Jing Guo, Ling Zhu, Chong Li, Juan Xie, Chunmeng Shi, Guoning Huang, Jingyu Li
Summary: In this study, the near-infrared fluorophore IR-61 was found to accumulate in oocyte mitochondria and improve mitochondrial function in postovulatory aging. It also rescued oocyte quality and embryonic developmental potential. RNA sequencing analysis indicated that IR-61 might inhibit the oxidative stress pathway induced by postovulatory aging.
Article
Biochemistry & Molecular Biology
Michele Mannelli, Tania Gamberi, Rachele Garella, Francesca Magherini, Roberta Squecco, Tania Fiaschi
Summary: Cachexia, a systemic disease associated with several pathologies, can be prevented in myotubes by the addition of sodium pyruvate in conditioned media from CT26 colon cancer cells. Sodium pyruvate inhibits the metabolic modifications responsible for the acquisition of cachectic features, and also decreases phosphorylation level of STAT3.
Article
Obstetrics & Gynecology
Usama Al-Zubaidi, Deepak Adhikari, Ozgur Cinar, Qing-Hua Zhang, Wai Shan Yuen, Michael P. Murphy, Luk Rombauts, Rebecca L. Robker, John Carroll
Summary: The study showed that MitoQ and BGP-15 during in vitro maturation can protect against spindle and chromosomal defects in oocytes exposed to oxidative stress and from aged mice. In human oocytes, MitoQ promotes nuclear maturation and protects against chromosomal misalignments. These findings suggest that mitochondrially targeted molecules could be a novel therapeutic approach against maternal aging-related spindle and chromosomal abnormalities.
HUMAN REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Guillaume Fle, Elijah Van Houten, Gaudeline Remillard-Labrosse, Greg FitzHarris, Guy Cloutier
Summary: In recent years, researchers have explored cellular biomechanical properties as a new method of oocyte selection in reproductive science. The reconstruction of viscoelastic parameter images in such materials remains a challenge. This study proposes a framework for mapping viscoelasticity at the subcellular scale and successfully applies it to live mouse oocytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Di Wu
Summary: The germinal vesicle (GV) stage is a critical transition point in mammalian oocyte development that determines oocyte quality through active RNA degradation and transcriptome reprofiling. Oocyte RNA-seq has revealed differences between non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) phases. Single oocyte RNA-seq (soRNA-seq) shows higher reproducibility and a better illustration of gene changes during oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Li Ge, Hong-Shu Sui, Guo-Cheng Lan, Na Liu, Jun-Zuo Wang, Jing-He Tan
FERTILITY AND STERILITY
(2008)
Article
Developmental Biology
Jun-Zuo Wang, Hong-Shu Sui, De-Qiang Miao, Na Liu, Ping Zhou, Li Ge, Jing-He Tan
Article
Cell Biology
Jing-Jing Li, Hua-yu Lian, Si-Yu Zhang, Wei Cui, Hong-Shu Sui, Dong Han, Na Liu, Jing-He Tan
Article
Biochemistry & Molecular Biology
Guangming Wu, Na Liu, Ina Rittelmeyer, Amar Deep Sharma, Malte Sgodda, Holm Zaehres, Martina Bleidissel, Boris Greber, Luca Gentile, Dong Wook Han, Cornelia Rudolph, Doris Steinemann, Axel Schambach, Michael Ott, Hans R. Schoeler, Tobias Cantz
Article
Developmental Biology
Na Liu, Fatimunnisa Qadri, Hauke Busch, Stefanie Huegel, Gabin Sihn, Ilya Chuykin, Enno Hartmann, Michael Bader, Franziska Rother
Summary: The protein Kpna6 plays a crucial role in spermatogenesis and male fertility, with its deficiency leading to infertility and various effects on sperm and Sertoli cells. Its absence results in impaired Sertoli cell function, incomplete sperm maturation, and altered expression of genes involved in sperm production.
Article
Biochemistry & Molecular Biology
Tian-Wu Qiao, Na Liu, De-Qiang Miao, Xia Zhang, Dong Han, Li Ge, Jing-He Tan
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2008)
