Article
Reproductive Biology
Ning Liu, Xuemeng Si, Yun Ji, Qing Yang, Jun Bai, Yu He, Hai Jia, Zhuan Song, Jingqing Chen, Ling Yang, Shenming Zeng, Ying Yang, Zhenlong Wu
Summary: Recent studies have shown that L-proline acts as an antioxidant to protect cells from oxidative stress. This study investigates the effect of proline on the developmental potential of mouse oocytes and its role in regulating glutathione-related redox homeostasis. The results demonstrate that proline supplementation enhances oocyte and embryo development, upregulates mRNA expression of key enzymes, increases glutathione concentration, and reduces reactive oxygen species levels in oocytes. Gap junctional communication and proline supplementation play important roles in maintaining redox balance and promoting embryo development.
Article
Reproductive Biology
Nazli Akin, Lucia von Mengden, Anamaria-Cristina Herta, Katy Billooye, Julia van Leersum, Berta Cava-Cami, Laura Saucedo-Cuevas, Fabio Klamt, Johan Smitz, Ellen Anckaert
Summary: The novel two-step IVM method CAPA-IVM has improved embryology outcomes by synchronizing oocyte nuclear and cytoplasmic maturation, although there is still a noticeable efficiency gap compared with traditional IVF. Glucose metabolism studies have revealed metabolic shortcomings within CAPA-IVM COCs, with specific alterations in mouse COC glucose metabolism characterized for the first time. Future optimization strategies for CAPA-IVM could focus on adjusting culture media energy substrate concentrations and/or implementing co-culture strategies.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Zhi Yin, Jing-Tao Sun, Hong-Di Cui, Chao-Qian Jiang, Yu-Ting Zhang, Sanghoon Lee, Zhong-Hua Liu, Jun-Xue Jin
Summary: The study showed that supplementation of 10 μg/mL tannic acid had beneficial effects on oocyte cytoplasmic maturation and subsequent embryonic development in pigs, while a high concentration of 100 μg/mL tannic acid might have negative impacts on cytoplasmic maturation and embryonic development.
Article
Cell Biology
G. D. Catandi, D. R. Bresnahan, S. O. Peters, K. J. Fresa, L. J. Maclellan, C. D. Broeckling, E. M. Carnevale
Summary: Many age-related changes in metabolite abundance suggest impaired mitochondrial metabolic function and oxidative stress in oocytes and follicular cells. The results also indicate impaired transfer of carbohydrate and free fatty acid substrates from cumulus cells to the oocytes of old mares, potentially related to disruption of transzonal projections between the cell types. The identification of age-associated alterations in the abundance of specific metabolites and their correlations among cells contribute to our understanding of follicular dysfunction with maternal aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Reproductive Biology
Seon Ah Ryu, Song Baek, Keun Cheon Kim, Eun Song Lee, Seung Tae Lee
Summary: The presence of cumulus cells is not essential for the completion of cytoplasmic maturation in oocytes after nuclear maturation. Removal of cumulus cells from porcine medium antral follicles (MAFs)-derived cumulus-oocyte complexes (COCs) after nuclear maturation results in significant improvements in cytoplasmic maturation and preimplantation development. The cumulus cells do not play a relevant role in cytoplasmic maturation of immature oocytes.
Article
Reproductive Biology
Zi-Bin Liu, Jia-Bao Zhang, Sheng-Peng Li, Wen-Jie Yu, Na Pei, Hai-Tao Jia, Ze Li, Wen-Fa Lv, Jun Wang, Nam-Hyung Kim, Bao Yuan, Hao Jiang
Summary: This study investigated the effects of inhibiting the expression of ID3 on cumulus cells (CCs) and uncovered the downstream regulatory network of ID3. The results showed that ID3 inhibition disrupted mitochondrial function, progesterone synthesis, and oocyte maturation. GO and KEGG analysis revealed the involvement of cholesterol-related processes and progesterone-mediated oocyte maturation. The study also found that ID3 inhibition led to increased apoptosis, inhibited phosphorylation of ERK1/2, and disrupted mitochondrial dynamics and function, resulting in poor oocyte maturation and quality. These findings contribute to the understanding of the biological roles of ID3 and cumulus cells.
Article
Reproductive Biology
Xiaotong Luo, Xuan Chen, Yanqiu Lv, Yue Han, Xinglin Qu, Yuyang Zhang, Xin Li, Yongsheng Yu, Yi Jin
Summary: This study compared miRNA expression in porcine cumulus cells before and after oocyte maturation using RNA-seq technology and found that miR-101 regulates oocyte maturation by targeting HAS2.
Article
Reproductive Biology
Ming Chen, Weizheng Yang, Yueshuai Guo, Xiaojing Hou, Shuai Zhu, Hongzheng Sun, Xuejiang Guo, Minjian Chen, Qiang Wang
Summary: In this study, the metabolic and proteomic profiling of cumulus cells during mouse oocyte maturation were analyzed. The findings revealed the active hyaluronic acid metabolism, steroid hormone synthesis, and prostaglandin E2 production in cumulus cells. Additionally, an increase in nucleotide and amino acid metabolism was observed in the surrounding cumulus cells as oocyte maturation progresses. These results provide valuable insights into the terminal differentiation of ovarian granulosa cells and have potential implications for improving and predicting oocyte quality.
JOURNAL OF OVARIAN RESEARCH
(2023)
Article
Environmental Sciences
Noelia Nikoloff, Ana C. Carranza Martin, Mariana C. Fabra, Cecilia C. Furnus
Summary: The study evaluated the genotoxic and cytotoxic effects of amitraz on bovine cumulus cells and oocyte nuclear maturation. Results showed that amitraz induced late apoptosis and necrotic cells at certain concentrations, while decreasing DNA damage. There was also a decrease in metaphase II and observation of degenerate oocytes at specific concentrations. No significant differences were found in oxidative stress parameters.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Cell Biology
Batara Sirait, Budi Wiweko, Ahmad Aulia Jusuf, Dein Iftitah, R. Muharam
Summary: This study examines several biomarkers in the cumulus-oocyte complex for their predictive significance in evaluating oocyte developmental competence and IVF outcomes. While these biomarkers show potential for predicting oocyte competency, challenges limit their practical application in IVF as prognostic tools.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Bing-Wang Zhao, Si-Min Sun, Ke Xu, Yuan-Yuan Li, Wen-Long Lei, Li Li, Sai-Li Liu, Ying-Chun Ouyang, Qing-Yuan Sun, Zhen-Bo Wang
Summary: This study uncovered the important role of F-box protein FBXO34 in oocyte meiotic maturation, where its depletion resulted in failure of meiotic resumption and overexpression promoted germinal vesicle breakdown. Additionally, overexpression of FBXO34 also led to continuous activation of the spindle assembly checkpoint and subsequent MI arrest in oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Anamaria-Cristina Herta, Lucia Mengden, Nazli Akin, Katy Billooye, Wim Coucke, Julia Leersum, Berta Cava-Cami, Laura Saucedo-Cuevas, Fabio Klamt, Johan Smitz, Ellen Anckaert
Summary: This study explored the glucose-related pathways in different follicle cell types and found altered metabolic patterns in in vitro-cultured mouse follicles, suggesting potential impacts on oocyte competence.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Ishani Dinisri, Sandarenu Kodikara, Madhusha Prasadani, Indunil Pathirana, Chathura Rathnayake, Basil Alexander, Kai-Fai Lee, Suranga Pradeep Kodithuwakku
Summary: Mancozeb negatively impacts caprine oocytes and granulosa cells, potentially impairing fertilization ability and developmental potential of the oocytes, and suppressing ovulation. It suppresses granulosa cells viability, reduces cumulus expansion, and suppresses metaphase plate formation in oocytes, while also inducing progesterone secretion from granulosa cells.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Review
Obstetrics & Gynecology
Dulama Richani, Kylie R. Dunning, Jeremy G. Thompson, Robert B. Gilchrist
Summary: Metabolism is crucial for oocyte quality, with glucose being essential for COC maturation and energy production. Mitochondria are the main ATP source in oocytes, while cumulus cells play a vital role in maintaining ATP levels. New insights in oocyte lipid metabolism and innovative strategies in IVM have shown positive effects on oocyte competence.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Biochemistry & Molecular Biology
Ralf Poehland, Jens Vanselow, Fabiana Melo Sterza
Summary: This study investigated the presence of HES1 in both compartments of bovine cumulus oocyte complexes (COCs) and its distribution after in vitro maturation (IVM). HES1 mRNA was detectable in oocytes but not in cumulus cells, while the protein was mainly visible in cumulus cells. GFP-HES1-mRNA injection showed signal in both oocytes and cumulus cells, suggesting protein transfer from oocytes to cumulus cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Na Liu, Yan-Guang Wu, Guo-Cheng Lan, Hong-Shu Sui, Li Ge, Jun-Zu Wang, Yong Liu, Tian-Wu Qiao, Jing-He Tan
Article
Developmental Biology
Jun-Zuo Wang, Hong-Shu Sui, De-Qiang Miao, Na Liu, Ping Zhou, Li Ge, Jing-He Tan
Article
Cell Biology
Jing-Jing Li, Hua-yu Lian, Si-Yu Zhang, Wei Cui, Hong-Shu Sui, Dong Han, Na Liu, Jing-He Tan
Article
Biochemistry & Molecular Biology
Guangming Wu, Na Liu, Ina Rittelmeyer, Amar Deep Sharma, Malte Sgodda, Holm Zaehres, Martina Bleidissel, Boris Greber, Luca Gentile, Dong Wook Han, Cornelia Rudolph, Doris Steinemann, Axel Schambach, Michael Ott, Hans R. Schoeler, Tobias Cantz
Article
Developmental Biology
Na Liu, Fatimunnisa Qadri, Hauke Busch, Stefanie Huegel, Gabin Sihn, Ilya Chuykin, Enno Hartmann, Michael Bader, Franziska Rother
Summary: The protein Kpna6 plays a crucial role in spermatogenesis and male fertility, with its deficiency leading to infertility and various effects on sperm and Sertoli cells. Its absence results in impaired Sertoli cell function, incomplete sperm maturation, and altered expression of genes involved in sperm production.
Article
Biochemistry & Molecular Biology
Tian-Wu Qiao, Na Liu, De-Qiang Miao, Xia Zhang, Dong Han, Li Ge, Jing-He Tan
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2008)