Article
Biochemistry & Molecular Biology
Haruhito A. Uchida, Tetsuharu Takatsuka, Yoshiko Hada, Ryoko Umebayashi, Hidemi Takeuchi, Kenichi Shikata, Venkateswaran Subramanian, Alan Daugherty, Jun Wada
Summary: The study found that edaravone can attenuate angiotensin II-induced abdominal aortic aneurysms and atherosclerosis through its antioxidant and anti-inflammatory effects.
Article
Multidisciplinary Sciences
Qi-Quan Huang, Yiwei Hang, Renee Doyle, Qinwen Mao, Deyu Fang, Richard M. Pope
Summary: Tregs are a potential therapeutic target in autoimmune diseases such as rheumatoid arthritis. However, the mechanisms responsible for maintaining Tregs in chronic inflammatory conditions are poorly understood.
Article
Biochemistry & Molecular Biology
Weibin Shi, Jing Li, Kelly Bao, Mei-Hua Chen, Zhenqi Liu
Summary: Comparing C3H-Ldlr(-/-) mice with C3H-Apoe(-/-) mice, we found that a Western diet and hyperlipidemia lead to the development of type 2 diabetes, regardless of genetic causes.
Article
Endocrinology & Metabolism
Biana Shtaif, Shay Henry Hornfeld, Michal Yackobovitch-Gavan, Moshe Phillip, Galia Gat-Yablonski
Summary: Using transgenic collagen type II-specific Sirt1 knockout (CKO) mice, this study investigated the role of Sirt1 in nutritional induced catch up growth (CUG), finding that CKO mice had less organized growth plate and reduced efficiency of CUG. Moreover, the increased weight in CKO mice was associated with differences in activity and brain function.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Tsvetelina Batsalova, Balik Dzhambazov
Summary: Collagen type II (COL2) is significantly affected by autoimmune responses in rheumatoid arthritis (RA), and posttranslational modifications (PTMs) of COL2 have been implicated in RA autoimmunity. The discovery of anti-citrullinated protein response in RA has led to improved diagnostic assays and classification criteria for the disease. Immunological tolerance induced by modified COL2 peptides is considered as a potential strategy for RA therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Integrative & Complementary Medicine
Ju-Yeon Park, Young-Won Kwon, Sun-Ah Kim, Sun-Dong Park, Chang-Hyun Kim, Jin-Hee Kim, Ju-Hee Lee
Summary: The study demonstrated the anti-arthritic effects of SC-E3 in a mouse model of type-II collagen-induced arthritis, showing reduced paw swelling, bone erosion, inflammatory cell infiltration, and inflammation. SC-E3 was found to inhibit pro-inflammatory cytokines and osteoclastogenesis, indicating its potential therapeutic use for rheumatoid arthritis.
JOURNAL OF INTEGRATIVE MEDICINE-JIM
(2021)
Article
Pharmacology & Pharmacy
Xiaolu Yu, Junnan Zhou, Fuli Zhao, Xuan Liu, Yuhang Mao, Li Diao, Chuanjun Wen, Mei Liu
Summary: Tomatidine (Td) shows inhibitory effects on inflammatory responses in arthritis and therapeutic effects on synovial inflammation and joint destruction in rats. Td can significantly suppress the production of pro-inflammatory cytokines and may act by reducing the activation of MAPKs and NF-κB.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Rui Xiong, Wenyang Jiang, Ning Li, Bohao Liu, Ruyuan He, Bo Wang, Qing Geng
Summary: The study found that exposure to PM2.5 can increase inflammatory response, oxidative stress, and apoptosis levels in lung tissue. Knockout of macrophage-specific NLRP3 gene can mitigate these effects.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Immunology
Nadine Szumilas, Odilia B. J. Corneth, Christian H. K. Lehmann, Heike Schmitt, Svenia Cunz, Jolie G. Cullen, Talyn Chu, Anita Marosan, Attila Mocsai, Vladimir Benes, Dietmar Zehn, Diana Dudziak, Rudi W. Hendriks, Lars Nitschke
Summary: Siglec-H regulates TLR-9-dependent inflammatory responses after virus infections, but not TLR-7 dependent responses. Lack of Siglec-H in pDCs leads to impaired Hck expression and downregulation of chemokine receptor CCR9, affecting pDC function.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Hiromi Nakai-Shimoda, Tatsuhito Himeno, Tetsuji Okawa, Emiri Miura-Yura, Sachiko Sasajima, Makoto Kato, Yuichiro Yamada, Yoshiaki Morishita, Shin Tsunekawa, Yoshiro Kato, Yusuke Seino, Rieko Inoue, Masaki Kondo, Susumu Seino, Keiko Naruse, Koichi Kato, Hiroki Mizukami, Jiro Nakamura, Hideki Kamiya
Summary: Glucose-responsive ATP-sensitive potassium channels (K-ATP) play important roles in the peripheral nervous system, affecting neurite outgrowth and nerve conduction velocity.
Article
Biochemistry & Molecular Biology
Fan Rui, Kang Jiawei, Hao Yuntao, Liu Xinran, Hu Jiani, Mao Ruixue, Liu Rui, Zhu Na, Xu Meihong, Li Yong
Summary: The study aimed to confirm the preventive and therapeutic effects of undenatured type II collagen (UC II) on osteoarthritis in aging db/db mice and T2DM patients. By UC II intervention, significant improvement in motor functions and serum biomarker levels was observed in aging db/db mice, as well as pain relief, enhanced physical function, and increased walking ability and mobility distance in T2DM patients with knee OA.
Article
Chemistry, Applied
Yadong Zhao, Kunyu Lu, Xinyue Piao, Yan Song, Libin Wang, Rusen Zhou, Pingping Gao, Heng Yen Khong
Summary: This study evaluated the effect of adding collagen on the gel properties of surimi and observed the influence of collagen type for the first time. Compared to type II, collagen type I has higher water solubility and more charged amino acids, which allows it to better intertwine with surimi myofibrillar proteins, resulting in higher exposure of protein functional domains, more conformational changes of myosin, and stronger formation of chemical forces among proteins. These improvements accelerate the gelation rate, leading to a well-stabilized surimi gel. Surimi gels containing collagen I have more compact structures with uniformly distributed smaller pores compared to those containing collagen II, resulting in higher water holding capacity and better texture. The fortification performance of collagen I in surimi gel and the elucidated interaction mechanism between collagen and myofibrillar protein will guide the further use of collagen as an effective additive in the food industry.
Article
Medicine, Research & Experimental
Alena Karnosova, Veronika Strnadova, Blanka Zelezna, Jaroslav Kunes, Petr Kasparek, Lenka Maletinska
Summary: A study on NPFFR2-deficient mice found that NPFFR2 is involved in energy balance and thermogenesis. This study reports the metabolic impact of NPFFR2 deficiency in male and female mice fed either a standard diet or a high-fat diet. It was found that NPFFR2 knockout mice showed severe glucose intolerance, especially when fed a high-fat diet. Male NPFFR2 knockout mice had lower body weights, white adipose tissues, and liver weight, while female knockout mice had reduced expression of Adra3(3 and Ppar gamma, inhibiting lipolysis in adipose tissue.
Article
Cell Biology
Lisa Gambarotto, Samuele Metti, Matteo Corpetti, Martina Baraldo, Patrizia Sabatelli, Silvia Castagnaro, Matilde Cescon, Bert Blaauw, Paolo Bonaldo
Summary: COL6-related myopathies (COL6-RM) are inherited muscle disorders caused by mutations of COL6 genes, characterized by early-onset muscle weakness. Targeting autophagy through dietary spermidine administration has shown beneficial effects in col6a1(-/-) mice and COL6-RM patients. A 100-day-long spermidine regimen rescues muscle strength in col6a1(-/-) mice and improves mitochondria and neuromuscular junction integrity, suggesting its potential application in clinical trials for COL6-RM.
Article
Multidisciplinary Sciences
Richard King, Zesen Lin, Ginette Balbin-Cuesta, Gregg Myers, Ann Friedman, Guojing Zhu, Beth McGee, Thomas L. Saunders, Ryo Kurita, Yukio Nakamura, James Douglas Engel, Pavan Reddy, Rami Khoriaty
Summary: The expression of full SEC23A protein from endogenous regulatory elements of Sec23b can completely rescue the SEC23B-deficient mouse phenotype, while erythroid-specific deletion of all four Sec23 alleles in mice results in mid-embryogenesis death with features of CDAII. The study also suggests a novel therapeutic strategy for CDAII by increasing the expression of SEC23A in human erythroid cells.
Article
Cell & Tissue Engineering
Lucia Beltran-Camacho, Margarita Jimenez-Palomares, Marta Rojas-Torres, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Sara Eslava-Alcon, Ma Carmen Perez-Segura, Ana Serrano, Borja Antequera-Gonzalez, Jose Angel Alonso-Pinero, Almudena Gonzalez-Rovira, Ma Jesus Extremera-Garcia, Manuel Rodriguez-Pinero, Rafael Moreno-Luna, Martin Rossel Larsen, Ma Carmen Duran-Ruiz
STEM CELL RESEARCH & THERAPY
(2020)
Article
Rheumatology
Pilar Alvarez, Juan Jesus Augustin, Esther Tamayo, Marcos Iglesias, Olga Acinas, Maria Angeles Mendiguren, Jose Andres Vazquez, Fernanda Genre, David San Segundo, Jesus Merino, Ramon Merino
ARTHRITIS & RHEUMATOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Daniela Cerezo-Wallis, Marta Contreras-Alcalde, Kevin Troule, Xavier Catena, Cynthia Mucientes, Tonantzin G. Calvo, Estela Canon, Cristina Tejedo, Paula C. Pennacchi, Sabrina Hogan, Peter Koelblinger, Hector Tejero, Andrew X. Chen, Nuria Ibarz, Osvaldo Grana-Castro, Lola Martinez, Javier Munoz, Pablo Ortiz-Romero, Jose L. Rodriguez-Peralto, Gonzalo Gomez-Lopez, Fatima Al-Shahrour, Raul Rabadan, Mitchell P. Levesque, David Olmeda, Maria S. Soengas
Article
Surgery
Marcos Iglesias, Saami Khalifian, Byoung C. Oh, Yichuan Zhang, Devin Miller, Sarah Beck, Gerald Brandacher, Giorgio Raimondi
Summary: Research shows that the combination of JAK/STAT inhibitor Tofacitinib and CTLA4-Ig can enhance the regulatory effect on transplant recipients, offering a promising strategy for improved management of transplanted patients.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Biochemistry & Molecular Biology
Daniela Cerezo-Wallis, Ivan Ballesteros
Summary: The article discusses the important role of neutrophils in various solid tumors, exploring their functional diversity, tumor specificity, and research on therapeutic enhancement.
Article
Immunology
Africa Martinez-Blanco, Marilu Dominguez-Pantoja, Maria Botia-Sanchez, Sonia Perez-Cabrera, Nerea Bello-Iglesias, Paula Carrillo-Rodriguez, Natividad Martin-Morales, Antonio Lario-Simon, Maria M. Perez-Sanchez-Canete, Laura Montosa-Hidalgo, Salvador Guerrero-Fernandez, Victoria M. Longobardo-Polanco, Sandra Redondo-Sanchez, Alberto Cornet-Gomez, Maria Torres-Saez, Ana Fernandez-Ibanez, Laura Terron-Camero, Eduardo Andres-Leon, Francisco O'Valle, Ramon Merino, Mercedes Zubiaur, Jaime Sancho
Summary: In a chronic graft-versus-host disease model, Cd38(-/-) mice exhibited milder lupus-like autoimmunity and lower levels of autoantibodies, along with decreased percentages of certain cell types compared to wild-type mice. This suggests that CD38 in B cells acts as a modulator receptor that controls autoimmune responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucia Beltran-Camacho, Margarita Jimenez-Palomares, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Marta Rojas-Torres, Sara Eslava-Alcon, Jose Angel Alonso-Pinero, Almudena Gonzalez-Rovira, Ma Jesus Extremera-Garcia, Rosario Conejero, Esther Doiz, Manuel Rodriguez-Pinero, Martin R. Larsen, Ma Carmen Duran-Ruiz
Summary: The study suggests that circulating angiogenic cells (CACs) have regenerative potential and can enhance blood flow recovery in mice with critical limb ischemia. Pre-stimulation of CACs with factors secreted by atherosclerotic plaques enhances their regenerative properties. Proteomics analysis identified potential proteins involved in the response to ischemia and the effect of CACs administration, indicating a possible mechanism for the therapeutic effects of CACs in CLI.
Article
Medicine, Research & Experimental
David Olmeda, Daniela Cerezo-Wallis, Cynthia Mucientes, Tonantzin G. Calvo, Estela Canon, Direna Alonso-Curbelo, Nuria Ibarz, Javier Munoz, Jose L. Rodriguez-Peralto, Pablo Ortiz-Romero, Sagrario Ortega, Maria S. Soengas
Summary: Through live imaging experiments in mouse models, this study identified double-stranded RNA mimics as effective inhibitors of neolymphangiogenesis, metastasis, and disease relapse. Mechanistically, these RNA mimics exert a suppressive function by involving cell-autonomous type I interferon signaling, which is not shared by FDA-approved antimelanoma treatments.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biochemical Research Methods
Nuria Colome, Joaquin Abian, Kerman Aloria, Jesus M. Arizmendi, Silvia Barcelo-Batllori, Sophie Braga-Lagache, Odile Burlet-Schiltz, Montse Carrascal, J. Ignacio Casal, Eduard Chicano-Galvez, Cristina Chiva, Luis Felipe Clemente, Felix Elortza, Josep M. Estanyol, Joaquin Fernandez-Irigoyen, Patricia Fernandez-Puente, Maria Jose Fidalgo, Carine Froment, Manuel Fuentes, Carlos Fuentes-Almagro, Marina Gay, Alexandre Hainard, Manfred Heller, Maria Luisa Hernandez, Nieves Ibarrola, Ibon Iloro, Thomas Kieselbach, Antonio Lario, Marie Locard-Paulet, Anabel Marina-Ramirez, Luna Martin, Esperanza Morato-Lopez, Javier Munoz, Rosana Navajas, M. Antonia Odena, Leticia Odriozola, Eliandre de Oliveira, Alberto Paradela, Carla Pasquarello, Vivian de los Rios, Cristina Ruiz-Romero, Eduard Sabido, Manuel Sanchez del Pino, Jaime Sancho, Enrique Santamaria, Christine Schaeffer-Reiss, Justine Schneider, Carolina de la Torre, M. Luz Valero, Marta Vilaseca, Shuai Wu, Linfeng Wu, Pilar Ximenez de Embun, Francesc Canals, Fernando J. Corrales
Summary: Global analysis of protein phosphorylation using mass spectrometry has become a powerful tool in biological and biomedical research, but it presents challenges such as low stoichiometry and the need for residue-specific information. The Proteomics Multicentric Experiment 11 (PME11) was launched to investigate the performance of phosphoprotein analysis protocols, with a reference sample distributed to 31 laboratories for evaluation. The results indicate the suitability of the PME11 reference sample for benchmarking and optimizing phosphoproteomics strategies while evaluating intra and inter laboratory performance.
JOURNAL OF PROTEOMICS
(2022)
Article
Cell Biology
Yinan Guo, Franka Messner, Sarah E. Beck, Marcos Iglesias Lozano, Hubert Schwelberger, Yichuan Zhang, Kai Kammers, Byoung Chol Oh, Elizabeth D. Greene, Gerald Brandacher, Kelvin G. M. Brockbank
Summary: This study compared the preservative capacity of gluconate-lactobionate-dextran (Unisol) solutions and histidine-tryptophan-ketoglutarate (HTK) solution. The results showed that Unisol-based solutions demonstrated faster rebeating time and better contractility after cardiac transplantation compared to HTK. The expression of proinflammatory markers was also reduced in Unisol-treated animals.
Article
Cell Biology
Derek VanDyke, Marcos Iglesias, Jakub Tomala, Arabella Young, Jennifer Smith, Joseph A. Perry, Edward Gebara, Amy R. Cross, Laurene S. Cheung, Arbor G. Dykema, Brian T. Orcutt-Jahns, Tereza Henclova, Jaroslav Golias, Jared Balolong, Luke M. Tomasovic, David Funda, Aaron S. Meyer, Drew M. Pardoll, Joanna Hester, Fadi Issa, Christopher A. Hunter, Mark S. Anderson, Jeffrey A. Bluestone, Giorgio Raimondi, Jamie B. Spangler
Summary: Researchers have developed a fusion protein called F5111 immunocytokine (IC) that selectively activates and expands Tregs, providing a potential treatment for autoimmune diseases. This approach overcomes the limitations of traditional IL-2 treatment by preferentially stimulating Tregs while limiting off-target immune cell activation.
Review
Immunology
Marcos Iglesias, Daniel C. Brennan, Christian P. Larsen, Giorgio Raimondi
Summary: Calcineurin inhibitors (CNI)-based therapy has been the standard treatment for preventing organ transplant rejection, but long-term use leads to significant side effects. Costimulation Blockade (CoB) therapy, which interrupts T cell costimulatory signals, has shown potential for better management of transplant recipients compared to CNI-based therapy. CTLA4-Ig, a biologic therapy, has been the most successful approach in this field. However, monotherapy with CTLA4-Ig is insufficient for inducing long-term allograft acceptance.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Paula Carrillo-Rodriguez, Jose-Angel Robles-Guirado, Adrian Cruz-Palomares, Miguel Angel Palacios-Pedrero, Elena Gonzalez-Paredes, Alex Mas-Ciurana, Carolina Franco-Herrera, Paloma A. Ruiz-de-Castroviejo-Teba, Antonio Lario, Victoria Longobardo, Laura Montosa-Hidalgo, Maria M. Perez-Sanchez-Canete, Maria-Mercedes Corzo-Corbera, Sandra Redondo-Sanchez, Ana-Belen Jodar, Francisco J. Blanco, Esther Zumaquero, Ramon Merino, Jaime Sancho, Mercedes Zubiaur
Summary: This study analyzed the protein composition and functional clustering of extracellular vesicles (EV) released by peritoneal exudate cells (PECs) in a pristane-induced lupus model. Various EV subtypes were identified and potential protein markers related to inflammation and resolution of chronic inflammation were identified.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Kelly R. Rhodes, Stephany Y. Tzeng, Marcos Iglesias, Dongwoo Lee, Kaitlyn Storm, Sarah Y. Neshat, Derek VanDyke, Shirley M. Lowmaster, Jamie B. Spangler, Giorgio Raimondi, Jordan J. Green
Summary: Multiple sclerosis (MS) is an autoimmune disease where immune cells damage myelinated nerves, resulting in impaired brain function. We developed a therapy using biodegradable microparticles loaded with rapamycin, an interleukin-2 fusion protein, and a myelin peptide to promote Treg expansion and activity. This therapy showed promising results in a mouse model of MS.