☆ 4.5 Article

Increased Fibroblast Cell Proliferation and Migration Using Atmospheric N2/Ar Micro-Plasma for the Stimulated Release of Fibroblast Growth Factor-7

PLASMA PROCESSES AND POLYMERS (2014)

Journal

PLASMA PROCESSES AND POLYMERS

Volume 11, Issue 1, Pages 80-88

Publisher

WILEY-V C H VERLAG GMBH

DOI: 10.1002/ppap.201300098

Keywords

cell culture; cell functions; fibroblast growth factor-7; N-2; Ar micro-plasma; optical emission spectroscopy; reactive oxygen species

Categories

Physics, Applied Physics, Fluids & Plasmas Physics, Condensed Matter Polymer Science

Funding

National Science Council of Taiwan [NSC 101-2321-B-006-012]

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Abstract

In situ assessment of cell functions on N-2/Ar micro-plasma exposed fibroblast cells is examined to better understand the effect of atmospheric low-dose plasma treatment. The cells number increased threefolds for plasma exposure time of 5 or 10s, followed by cell culture for 48h. The cell coverage rate rose 20% for the same plasma exposure time, followed by cell culture for 6 or 12h. 0.5% N-2/Ar micro-plasma exposure can particularly be used to achieve the stimulated release of FGF7 and subsequent enhancement of cells proliferation and migration. This work thus provides a potential of using micro-plasma system for cells related studies and the improvement of cutaneous wound healing.

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