High-content screening technology for studying drug-induced hepatotoxicity in cell models

A simple transcriptomic signature able to predict drug-induced hepatic steatosis

(2014) Marta Benet et al.   ARCHIVES OF TOXICOLOGY

A testing strategy to predict risk for drug-induced liver injury in humans using high-content screen assays and the ‘rule-of-two’ model

(2014) Minjun Chen et al.   ARCHIVES OF TOXICOLOGY

High-Content Analysis/Screening for Predictive Toxicology: Application to Hepatotoxicity and Genotoxicity

(2014) Mikael Persson et al.   BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY

High-Content Analysis in Toxicology: Screening Substances for Human Toxicity Potential, Elucidating Subcellular Mechanisms andIn VivoUse as Translational Safety Biomarkers

(2014) Peter J. O'Brien   BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY

Organ-on-a-chip platforms for studying drug delivery systems

(2014) Nupura S. Bhise et al.   JOURNAL OF CONTROLLED RELEASE

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

High Content Analysis Provides Mechanistic Insights on the Pathways of Toxicity Induced by Amine-Modified Polystyrene Nanoparticles

(2014) Sergio Anguissola et al.   PLoS One

Drug-Induced Hepatic Steatosis

(2014) Naga Chalasani et al.   SEMINARS IN LIVER DISEASE

Integrated testing strategies for safety assessments

(2014) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

HepG2 cells simultaneously expressing five P450 enzymes for the screening of hepatotoxicity: identification of bioactivable drugs and the potential mechanism of toxicity involved

(2013) Laia Tolosa et al.   ARCHIVES OF TOXICOLOGY

Validation of a HTS-amenable assay to detect drug-induced mitochondrial toxicity in the absence and presence of cell death

(2013) Rachel Swiss et al.   TOXICOLOGY IN VITRO

Evaluation of the use of imaging parameters for the detection of compound-induced hepatotoxicity in 384-well cultures of HepG2 cells and cryopreserved primary human hepatocytes

(2013) Helen Garside et al.   TOXICOLOGY IN VITRO

Towards a three-dimensional microfluidic liver platform for predicting drug efficacy and toxicity in humans

(2013) Abhinav Bhushan et al.   Stem Cell Research & Therapy

A high content screening assay to predict human drug-induced liver injury during drug discovery

(2013) Mikael Persson et al.   JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS

Drug-induced Liver Steatosis and Phospholipidosis: Cell-Based Assays for Early Screening of Drug Candidates

(2012) M. Teresa Donato et al.   CURRENT DRUG METABOLISM

Comparisons betweenin vitrowhole cell imaging andin vivozebrafish-based approaches for identifying potential human hepatotoxicants earlier in pharmaceutical development

(2012) Adrian Hill et al.   DRUG METABOLISM REVIEWS

Mitochondria and cell signalling

(2012) Stephen W. G. Tait et al.   JOURNAL OF CELL SCIENCE

Differential Mitochondrial Toxicity Screening and Multi-Parametric Data Analysis

(2012) Maria V. Tsiper et al.   PLoS One

Development of a Multiparametric Cell-based Protocol to Screen and Classify the Hepatotoxicity Potential of Drugs

(2012) Laia Tolosa et al.   TOXICOLOGICAL SCIENCES

Cytotoxic effects of 109 reference compounds on rat H4IIE and human HepG2 hepatocytes. III: Mechanistic assays on oxygen consumption with MitoXpress and NAD(P)H production with Alamar Blue™

(2012) Willem G.E.J. Schoonen et al.   TOXICOLOGY IN VITRO

Real-time concurrent monitoring of apoptosis, cytosolic calcium, and mitochondria permeability transition for hypermulticolor high-content screening of drug-induced mitochondrial dysfunction-mediated hepatotoxicity

(2012) Jae-Ah Kim et al.   TOXICOLOGY LETTERS

Central role of mitochondria in drug-induced liver injury

(2011) Dominique Pessayre et al.   DRUG METABOLISM REVIEWS

Induction of vesicular steatosis by amiodarone and tetracycline is associated with up-regulation of lipogenic genes in heparg cells

(2011) Sébastien Anthérieu et al.   HEPATOLOGY

Development and validation of a high-content screening in vitro micronucleus assay in CHO-k1 and HepG2 cells

(2011) Walter M.A. Westerink et al.   MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

Automated detection of hepatotoxic compounds in human hepatocytes using HepaRG cells and image-based analysis of mitochondrial dysfunction with JC-1 dye

(2011) K. Pernelle et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Comparison of a genomic and a multiplex cell imaging approach for the detection of phospholipidosis

(2011) K. Tilmant et al.   TOXICOLOGY IN VITRO

High content screening analysis of phospholipidosis: Validation of a 96-well assay with CHO-K1 and HepG2 cells for the prediction of in vivo based phospholipidosis

(2011) F.M. van de Water et al.   TOXICOLOGY IN VITRO

Evaluation and validation of multiple cell lines and primary mouse macrophages to predict phospholipidosis potential

(2011) Lloyd LeCureux et al.   TOXICOLOGY IN VITRO

Upgrading cytochrome P450 activity in HepG2 cells co-transfected with adenoviral vectors for drug hepatotoxicity assessment

(2011) Laia Tolosa et al.   TOXICOLOGY IN VITRO

Enhanced oxidative stress and increased mitochondrial mass during Efavirenz-induced apoptosis in human hepatic cells

(2010) N Apostolova et al.   BRITISH JOURNAL OF PHARMACOLOGY

In Vitro Evaluation of Potential Hepatotoxicity Induced by Drugs

(2010) M.J. Gomez-Lechon et al.   CURRENT PHARMACEUTICAL DESIGN

Cell viability assessment: toward content-rich platforms

(2010) Christina Nicole Ramirez et al.   Expert Opinion on Drug Discovery

Drug-induced toxicity on mitochondria and lipid metabolism: Mechanistic diversity and deleterious consequences for the liver

(2010) Karima Begriche et al.   JOURNAL OF HEPATOLOGY

Cytometric analysis for drug-induced steatosis in HepG2 cells

(2009) M. Teresa Donato et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Applications of High Content Screening in Life Science Research

(2009) Joseph Zock   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

In vitroassay for drug-induced hepatosteatosis using rat primary hepatocytes, a fluorescent lipid analog and gene expression analysis

(2009) Hisako Fujimura et al.   JOURNAL OF APPLIED TOXICOLOGY

Role of Hepatic Transporters in the Disposition and Hepatotoxicity of a HER2 Tyrosine Kinase Inhibitor CP-724,714

(2009) Bo Feng et al.   TOXICOLOGICAL SCIENCES

Synergistic drug–cytokine induction of hepatocellular death as an in vitro approach for the study of inflammation-associated idiosyncratic drug hepatotoxicity

(2009) Benjamin D. Cosgrove et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Relationship between in vitro phospholipidosis assay using HepG2 cells and 2-week toxicity studies in rats

(2009) Saku Miyamoto et al.   TOXICOLOGY MECHANISMS AND METHODS

High-Content Screening as a Universal Tool for Fingerprinting of Cytotoxicity of Nanoparticles

(2008) Edward Jan et al.   ACS Nano

Phospholipidosis Assay in HepG2 Cells and Rat or Rhesus Hepatocytes Using Phospholipid Probe NBD-PE

(2008) Neetesh Bhandari et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Drug-induced liver injury through mitochondrial dysfunction: mechanisms and detection during preclinical safety studies

(2008) Gilles Labbe et al.   FUNDAMENTAL & CLINICAL PHARMACOLOGY

In Vitro Assessment of Mitochondrial Dysfunction and Cytotoxicity of Nefazodone, Trazodone, and Buspirone

(2008) James A. Dykens et al.   TOXICOLOGICAL SCIENCES

Cellular Imaging Predictions of Clinical Drug-Induced Liver Injury

(2008) Jinghai J. Xu et al.   TOXICOLOGICAL SCIENCES

High concentrations of stavudine impair fatty acid oxidation without depleting mitochondrial DNA in cultured rat hepatocytes

(2008) Anissa Igoudjil et al.   TOXICOLOGY IN VITRO