Modulating protein stability - directed evolution strategies for improved protein function

Improved Thermostability of Clostridium thermocellum Endoglucanase Cel8A by Using Consensus-Guided Mutagenesis

(2012) Michael Anbar et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Increasing activity and thermal resistance ofBacillus gibsoniialkaline protease (BgAP) by directed evolution

(2012) Ronny Martinez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improved thermostability of AEH by combining B-FIT analysis and structure-guided consensus method

(2012) Janna K. Blum et al.   JOURNAL OF BIOTECHNOLOGY

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica

(2012) Sai Wen et al.   JOURNAL OF BIOTECHNOLOGY

Protein Quality Control Acts on Folding Intermediates to Shape the Effects of Mutations on Organismal Fitness

(2012) Shimon Bershtein et al.   MOLECULAR CELL

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Chemical chaperones assist intracellular folding to buffer mutational variations

(2012) Anannya Bandyopadhyay et al.   Nature Chemical Biology

Evolutionary transitions to new DNA methyltransferases through target site expansion and shrinkage

(2012) Liat Rockah-Shmuel et al.   NUCLEIC ACIDS RESEARCH

Assessing Predictors of Changes in Protein Stability upon Mutation Using Self-Consistency

(2012) Grant Thiltgen et al.   PLoS One

PROTS-RF: A Robust Model for Predicting Mutation-Induced Protein Stability Changes

(2012) Yunqi Li et al.   PLoS One

Soluble oligomerization provides a beneficial fitness effect on destabilizing mutations

(2012) S. Bershtein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Global analysis of chaperone effects using a reconstituted cell-free translation system

(2012) T. Niwa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59

(2012) O. Khersonsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Advanced evolutionary molecular engineering to produce thermostable cellulase by using a small but efficient library

(2012) Y. Ito et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Highly thermostable fungal cellobiohydrolase I (Cel7A) engineered using predictive methods

(2012) Russell S. Komor et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Increased thermostability of microbial transglutaminase by combination of several hot spots evolved by random and saturation mutagenesis

(2011) Karin Buettner et al.   AMINO ACIDS

Directed evolution of a highly active Yersinia mollaretii phytase

(2011) Amol V. Shivange et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

PoPMuSiC 2.1: a web server for the estimation of protein stability changes upon mutation and sequence optimality

(2011) Yves Dehouck et al.   BMC BIOINFORMATICS

Protein stability by number: high-throughput and statistical approaches to one of protein science's most difficult problems

(2011) Thomas J Magliery et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Utilizing natural diversity to evolve protein function: applications towards thermostability

(2011) Megan F Cole et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Strategy and success for the directed evolution of enzymes

(2011) Paul A Dalby   CURRENT OPINION IN STRUCTURAL BIOLOGY

The Transition of Human Estrogen Sulfotransferase from Generalist to Specialist Using Directed Enzyme Evolution

(2011) Dotan Amar et al.   JOURNAL OF MOLECULAR BIOLOGY

SNPeffect 4.0: on-line prediction of molecular and structural effects of protein-coding variants

(2011) G. De Baets et al.   NUCLEIC ACIDS RESEARCH

SDM--a server for predicting effects of mutations on protein stability and malfunction

(2011) C. L. Worth et al.   NUCLEIC ACIDS RESEARCH

Computational design and selections for an engineered, thermostable terpene synthase

(2011) Juan E. Diaz et al.   PROTEIN SCIENCE

The linear interaction energy method for the prediction of protein stability changes upon mutation

(2011) Lauren Wickstrom et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Genetic Selection for Enhanced Folding In Vivo Targets the Cys14-Cys38 Disulfide Bond in Bovine Pancreatic Trypsin Inhibitor

(2010) Linda Foit et al.   ANTIOXIDANTS & REDOX SIGNALING

Protein kinetic stability

(2010) Jose M. Sanchez-Ruiz   BIOPHYSICAL CHEMISTRY

Chemical and Pharmacological Chaperones: Application for Recombinant Protein Production and Protein Folding Diseases

(2010) Rahul S. Rajan et al.   CURRENT MEDICINAL CHEMISTRY

A systematic survey of in vivo obligate chaperonin-dependent substrates

(2010) Kei Fujiwara et al.   EMBO JOURNAL

Performance of protein stability predictors

(2010) Sofia Khan et al.   HUMAN MUTATION

Enhancing the Stability and Solubility of the Glucocorticoid Receptor Ligand-Binding Domain by High-Throughput Library Screening

(2010) Tobias Seitz et al.   JOURNAL OF MOLECULAR BIOLOGY

Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast

(2010) Eva García-Ruiz et al.   Microbial Cell Factories

RosettaBackrub--a web server for flexible backbone protein structure modeling and design

(2010) F. Lauck et al.   NUCLEIC ACIDS RESEARCH

In vivo protein stabilization based on fragment complementation and a split GFP system

(2010) S. Lindman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improvement of Bacillus circulans β-amylase activity attained using the ancestral mutation method

(2010) Kan Yamashiro et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A survey of proteins encoded by non-synonymous single nucleotide polymorphisms reveals a significant fraction with altered stability and activity

(2009) Abdellah Allali-Hassani et al.   BIOCHEMICAL JOURNAL

Directed Evolution of Serum Paraoxonase PON3 by Family Shuffling and Ancestor/Consensus Mutagenesis, and Its Biochemical Characterization

(2009) Olga Khersonsky et al.   BIOCHEMISTRY

Stability effects of mutations and protein evolvability

(2009) Nobuhiko Tokuriki et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Disaggregating Chaperones: An Unfolding Story

(2009) Sandeep Sharma et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series

(2009) Olga Khersonsky et al.   JOURNAL OF MOLECULAR BIOLOGY

Optimizing Protein Stability In Vivo

(2009) Linda Foit et al.   MOLECULAR CELL

Chaperonin overexpression promotes genetic variation and enzyme evolution

(2009) Nobuhiko Tokuriki et al.   NATURE

Finding better protein engineering strategies

(2009) Romas J Kazlauskas et al.   Nature Chemical Biology

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Converging concepts of protein folding in vitro and in vivo

(2009) F Ulrich Hartl et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assessing computational methods for predicting protein stability upon mutation: good on average but not in the details

(2009) V. Potapov et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Relative Tolerance of an Enzymatic Molten Globule and Its Thermostable Counterpart to Point Mutation†

(2008) Kenneth J. Woycechowsky et al.   BIOCHEMISTRY

Rational improvement of simvastatin synthase solubility inEscherichia colileads to higher whole-cell biocatalytic activity

(2008) Xinkai Xie et al.   BIOTECHNOLOGY AND BIOENGINEERING

Evolutionary History of a Specialized P450 Propane Monooxygenase

(2008) Rudi Fasan et al.   JOURNAL OF MOLECULAR BIOLOGY

Increased Folding Stability of TEM-1 β-Lactamase by In Vitro Selection

(2008) Insa Kather et al.   JOURNAL OF MOLECULAR BIOLOGY

Intense Neutral Drifts Yield Robust and Evolvable Consensus Proteins

(2008) Shimon Bershtein et al.   JOURNAL OF MOLECULAR BIOLOGY

Predicting free energy changes using structural ensembles

(2008) Alexander Benedix et al.   NATURE METHODS

SPROUTS: a database for the evaluation of protein stability upon point mutation

(2008) Mathieu Lonquety et al.   NUCLEIC ACIDS RESEARCH

Identification of soluble protein fragments by gene fragmentation and genetic selection

(2008) Michael R. Dyson et al.   NUCLEIC ACIDS RESEARCH

New Molecular Reporters for Rapid Protein Folding Assays

(2008) Stéphanie Cabantous et al.   PLoS One

How Protein Stability and New Functions Trade Off

(2008) Nobuhiko Tokuriki et al.   PLoS Computational Biology

Engineering proteins with tunable thermodynamic and kinetic stabilities

(2007) Angel L. Pey et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS