Fluorescent Proteins as Genetically Encoded FRET Biosensors in Life Sciences

Type I cell ROS kinetics under hypoxia in the intact mouse carotid body ex vivo: a FRET-based study

(2015) A. Bernardini et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

ROZA-XL, an improved FRET based biosensor with an increased dynamic range for visualizing Zeta Associated Protein 70 kD (ZAP-70) tyrosine kinase activity in live T cells

(2015) Sophie Cadra et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Application of a fluorescence resonance energy transfer (FRET)-based biosensor for detection of drug-induced apoptosis in a 3D breast tumor model

(2015) Padmaja Anand et al.   BIOTECHNOLOGY AND BIOENGINEERING

Dynamic visualization of calcium-dependent signaling in cellular microdomains

(2015) Sohum Mehta et al.   CELL CALCIUM

Understanding FRET as a Research Tool for Cellular Studies

(2015) Dilip Shrestha et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin

(2015) Toru Hiratsuka et al.   eLife

Development of Fluorescent Substrates and Assays for the Key Autophagy-Related Cysteine Protease Enzyme, ATG4B

(2014) Thanh G. Nguyen et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Shining light on cell death processes - a novel biosensor for necroptosis, a newly described cell death program

(2014) François Sipieter et al.   Biotechnology Journal

The spatio-temporal dynamics of PKA activity profile during mitosis and its correlation to chromosome segregation

(2014) Pauline Vandame et al.   CELL CYCLE

How to Measure Molecular Forces in Cells: A Guide to Evaluating Genetically-Encoded FRET-Based Tension Sensors

(2014) Anna-Lena Cost et al.   Cellular and Molecular Bioengineering

A Genetically-Encoded YFP Sensor with Enhanced Chloride Sensitivity, Photostability and Reduced pH Interference Demonstrates Augmented Transmembrane Chloride Movement by Gerbil Prestin (SLC26a5)

(2014) Sheng Zhong et al.   PLoS One

The Use of a Stably Expressed FRET Biosensor for Determining the Potency of Cancer Drugs

(2014) William P. Bozza et al.   PLoS One

Optimization of ERK Activity Biosensors for both Ratiometric and Lifetime FRET Measurements

(2014) Pauline Vandame et al.   SENSORS

Monitoring of dual bio-molecular events using FRET biosensors based on mTagBFP/sfGFP and mVenus/mKOκ fluorescent protein pairs

(2013) Ting Su et al.   BIOSENSORS & BIOELECTRONICS

Intravital FLIM-FRET Imaging Reveals Dasatinib-Induced Spatial Control of Src in Pancreatic Cancer

(2013) M. Nobis et al.   CANCER RESEARCH

Development of a FRET Biosensor with High Specificity for Akt

(2013) Haruko Miura et al.   CELL STRUCTURE AND FUNCTION

Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension across VE-Cadherin and PECAM-1

(2013) Daniel E. Conway et al.   CURRENT BIOLOGY

Single-fluorophore biosensors based on conformation-sensitive GFP variants

(2013) Agnès Bonnot et al.   FASEB JOURNAL

Fluorescence Resonance Energy Transfer Based on Interaction of PII and PipX Proteins Provides a Robust and Specific Biosensor for 2-Oxoglutarate, a Central Metabolite and a Signaling Molecule

(2013) Hai-Lin Chen et al.   FEBS Journal

Stochastic ERK Activation Induced by Noise and Cell-to-Cell Propagation Regulates Cell Density-Dependent Proliferation

(2013) Kazuhiro Aoki et al.   MOLECULAR CELL

Quantitative FRET Imaging to Visualize the Invasiveness of Live Breast Cancer Cells

(2013) Shaoying Lu et al.   PLoS One

A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the Warburg Effect in Single Cancer Cells

(2013) Alejandro San Martín et al.   PLoS One

Simultaneous Quantitative Live Cell Imaging of Multiple FRET-Based Biosensors

(2013) Andrew Woehler   PLoS One

Parallelized TCSPC for Dynamic Intravital Fluorescence Lifetime Imaging: Quantifying Neuronal Dysfunction in Neuroinflammation

(2013) Jan Leo Rinnenthal et al.   PLoS One

Distinct biophysical mechanisms of focal adhesion kinase mechanoactivation by different extracellular matrix proteins

(2013) J. Seong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells

(2013) Sara M. Müller et al.   Frontiers in Plant Science

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

(2013) Jason D. Vevea et al.   Jove-Journal of Visualized Experiments

A high-throughput FRET-based assay for determination of Atg4 activity

(2012) Min Li et al.   Autophagy

The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model

(2012) Katsuya Kominami et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor

(2012) Janko Potzkei et al.   BMC BIOLOGY

Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors

(2012) Yuji Kamioka et al.   CELL STRUCTURE AND FUNCTION

Monitoring Biosensor Activity in Living Cells with Fluorescence Lifetime Imaging Microscopy

(2012) Julia Hum et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Real-time hyperspectral fluorescence imaging of pancreatic  -cell dynamics with the image mapping spectrometer

(2012) A. D. Elliott et al.   JOURNAL OF CELL SCIENCE

Red-shifted fluorescent proteins monitor enzymatic activity in live HT-1080 cells with fluorescence lifetime imaging microscopy (FLIM)

(2012) J.P. EICHORST et al.   JOURNAL OF MICROSCOPY

Fluorescence lifetime imaging - techniques and applications

(2012) W. BECKER   JOURNAL OF MICROSCOPY

Six-Color Time-Resolved Förster Resonance Energy Transfer for Ultrasensitive Multiplexed Biosensing

(2012) Daniel Geißler et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

(2012) Hellen C. Ishikawa-Ankerhold et al.   MOLECULES

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

In Vivo Imaging of Hierarchical Spatiotemporal Activation of Caspase-8 during Apoptosis

(2012) Katsuya Kominami et al.   PLoS One

E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch

(2012) N. Borghi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Apoptotic and Nonapoptotic Caspase Functions in Animal Development

(2012) M. Miura   Cold Spring Harbor Perspectives in Biology

Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure

(2011) Yoshifumi Yamaguchi et al.   JOURNAL OF CELL BIOLOGY

Pancreatic cancer

(2011) Audrey Vincent et al.   LANCET

Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy

(2011) Elizabeth Hinde et al.   MICROSCOPY RESEARCH AND TECHNIQUE

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

(2011) Michele L. Markwardt et al.   PLoS One

Some Secrets of Fluorescent Proteins: Distinct Bleaching in Various Mounting Fluids and Photoactivation of Cyan Fluorescent Proteins at YFP-Excitation

(2011) Naila Malkani et al.   PLoS One

Extracellular Matrix Degradation and Remodeling in Development and Disease

(2011) P. Lu et al.   Cold Spring Harbor Perspectives in Biology

Reversible Dimerization ofAequorea victoriaFluorescent Proteins Increases the Dynamic Range of FRET-Based Indicators

(2010) Ippei Kotera et al.   ACS Chemical Biology

Förster distances for fluorescence resonant energy transfer between mCherry and other visible fluorescent proteins

(2010) Nina Akrap et al.   ANALYTICAL BIOCHEMISTRY

Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters

(2010) T. Drepper et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A Novel FRET-Based Biosensor for the Measurement of BCR-ABL Activity and Its Response to Drugs in Living Cells

(2010) T. Mizutani et al.   CLINICAL CANCER RESEARCH

Progressive Activation of CyclinB1-Cdk1 Coordinates Entry to Mitosis

(2010) Olivier Gavet et al.   DEVELOPMENTAL CELL

Dasatinib Inhibits the Development of Metastases in a Mouse Model of Pancreatic Ductal Adenocarcinoma

(2010) Jennifer P. Morton et al.   GASTROENTEROLOGY

Fluorescence lifetime imaging microscopy in life sciences

(2010) Jan Willem Borst et al.   MEASUREMENT SCIENCE and TECHNOLOGY

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

(2010) Carsten Grashoff et al.   NATURE

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

(2010) Joachim Goedhart et al.   NATURE METHODS

Discovery of Green Fluorescent Protein (GFP) (Nobel Lecture)

(2009) Osamu Shimomura   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Phosphorylation-Driven Assembly of the RIP1-RIP3 Complex Regulates Programmed Necrosis and Virus-Induced Inflammation

(2009) YoungSik Cho et al.   CELL

Genetically encoded biosensors based on engineered fluorescent proteins

(2009) Wolf B. Frommer et al.   CHEMICAL SOCIETY REVIEWS

Genetically Encoded Forster Resonance Energy Transfer Sensors for the Conformation of the Src Family Kinase Lck

(2009) W. Paster et al.   JOURNAL OF IMMUNOLOGY

FRET Quenching of Photosensitizer Singlet Oxygen Generation

(2009) Jonathan F. Lovell et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Analysis of the matrix metalloproteinase family reveals that MMP8 is often mutated in melanoma

(2009) Lavanya H Palavalli et al.   NATURE GENETICS

mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures

(2009) Simone Kredel et al.   PLoS One

Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators

(2009) H. Imamura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RIP3, an Energy Metabolism Regulator That Switches TNF-Induced Cell Death from Apoptosis to Necrosis

(2009) D.-W. Zhang et al.   SCIENCE

Matrix Metalloproteinase-8 Functions as a Metastasis Suppressor through Modulation of Tumor Cell Adhesion and Invasion

(2008) A. Gutierrez-Fernandez et al.   CANCER RESEARCH

Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation

(2008) Johan F. Paulsson et al.   Experimental Diabetes Research

Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase Activity in Live Cells by Fluorescence Resonance Energy Transfer Imaging

(2008) Mingxing Ouyang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design

(2008) Jasper Akerboom et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Atg8 Controls Phagophore Expansion during Autophagosome Formation

(2008) Zhiping Xie et al.   MOLECULAR BIOLOGY OF THE CELL

Autophagy fights disease through cellular self-digestion

(2008) Noboru Mizushima et al.   NATURE

Mechanotransduction in vascular physiology and atherogenesis

(2008) Cornelia Hahn et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A genetically encoded ratiometric sensor to measure extracellular pH in microdomains bounded by basolateral membranes of epithelial cells

(2008) Javier Urra et al.   PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY

A Novel ZAP-70 Dependent FRET Based Biosensor Reveals Kinase Activity at both the Immunological Synapse and the Antisynapse

(2008) Clotilde Randriamampita et al.   PLoS One

A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

(2008) Gerard N. M. van der Krogt et al.   PLoS One

A genetically encoded fluorescent sensor of ERK activity

(2008) C. D. Harvey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters

(2008) S. A. Hires et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA