Article
Biology
Lingli Huang, Wenqing Li, Xingxing Dai, Shuai Zhao, Bo Xu, Fengsong Wang, Ren-Tao Jin, Lihua Luo, Limin Wu, Xue Jiang, Yu Cheng, Jiaqi Zou, Caoling Xu, Xianhong Tong, Heng-Yu Fan, Han Zhao, Jianqiang Bao
Summary: This study identified and characterized novel biallelic variants in MAD2L1BP responsible for oocyte maturation arrest at MI, providing new therapeutic avenues for female primary infertility.
Article
Cell Biology
Lili Guo, Yongda Zhao, Yanjun Huan
Summary: The study showed that chlorpyrifos had toxic effects on porcine oocytes, including delaying meiotic progression, decreasing polar body extrusion, disrupting spindle assembly and causing DNA damage, as well as impairing cytoplasmic maturation. Pterostilbene was able to effectively alleviate the damage caused by chlorpyrifos and improve oocyte maturation and embryonic development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Ranjha Khan, Xiaohua Jiang, Uzma Hameed, Qinghua Shi
Summary: The quality of oocytes from obese women can be compromised due to imbalanced lipid metabolism, which is crucial for oocyte development and maturation. Lipids play a significant role in various biological processes of oocyte development and are important for determining oocyte quality and cryopreservation for fertilization.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Meina He, Tuo Zhang, Yi Yang, Chao Wang
Summary: Meiosis is essential for sexual reproduction, with oocytes in female mammals beginning meiosis before birth and sustaining it through dictyate stage before ovulation. Fine-tuning of meiotic progression is crucial for fertility, as any disruption can lead to oogenesis failure and impact overall reproductive health. Understanding the regulatory network of oocyte meiosis is key to comprehending mammalian reproduction and the consequences of misregulation on infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Zhi-Yong Yang, Min Ye, Ya-Xin Xing, Qi-Gui Xie, Jian-Hong Zhou, Xin-Rui Qi, Kehkooi Kee, Ri-Cheng Chian
Summary: By performing single-cell analysis, this study identified the differentially expressed genes (DEGs) and pathways related to mitochondria during human oocyte maturation. The results showed that oxidative phosphorylation, pyruvate metabolism, peroxisome, and amino acid metabolism pathways played important roles in oocyte maturation. Additionally, it was observed that mitochondria were regulated by nuclear genes to meet the developmental needs, and the current in vitro maturation culture medium should be optimized based on these findings.
Article
Cell Biology
Long Jin, Hai-Ying Zhu, Xiang-Jin Kang, Li-Ping Lin, Pu-Yao Zhang, Tao Tan, Yang Yu, Yong Fan
Summary: Oxybenzone, a common UV filter in sunscreens and cosmetics, has been found to have negative effects on oocyte maturation, but melatonin shows protective effects against these detrimental effects.
Article
Reproductive Biology
Jianqiang Sun, Jian Li, Yaodi Wang, Jiadan Qu, Fanglong Bi, Hongxiao Xiang, Xintao Zhao, Mingju Sun, Yanjun Huan
Summary: This study found that cypermethrin (CYP) has a toxic effect on porcine oocyte maturation, while astaxanthin (AST) can protect oocyte maturation. CYP causes DNA damage and endoplasmic reticulum dysfunction in oocytes, while AST can alleviate these defects. This study provides a potential strategy to prevent pesticide toxicity and protect oocyte maturation in mammalian reproduction.
Article
Cell Biology
Song-Hee Lee, Ming-Hong Sun, Dongjie Zhou, Wen-Jie Jiang, Xiao-Han Li, Geun Heo, Xiang-Shun Cui
Summary: Exposure to high temperature has been found to negatively affect the development of porcine oocytes. It induces mitochondrial dysfunction, ER stress, abnormal Golgi apparatus distribution, and lysosomal damage, leading to the failure of meiotic maturation in the oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Fisheries
Robie Allan Bombardelli, Juliana Kasper Mewes, Alexandre Henrique Buzzi, Ahiana Cassia de Oliveira Pedreira, Mirna Adriane Syperreck, Anna Cristina Strieder Dalmaso, Tais Voelkl Chagas, Rogerio Jose Chiella, Fabio Meurer
Summary: The study evaluated the effects of feeding Nile tilapia broodstock with rations containing different levels of crude glycerin, finding that crude glycerin in the feed can have negative impacts on females by decreasing egg production and hatching rates.
Review
Cell Biology
Batara Sirait, Budi Wiweko, Ahmad Aulia Jusuf, Dein Iftitah, R. Muharam
Summary: This study examines several biomarkers in the cumulus-oocyte complex for their predictive significance in evaluating oocyte developmental competence and IVF outcomes. While these biomarkers show potential for predicting oocyte competency, challenges limit their practical application in IVF as prognostic tools.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Huaxing Zhao, Xiaohua He, Xianjun Zhang, Junsong Shi, Rong Zhou, Ranbiao Mai, Qiaoyun Su, Gengyuan Cai, Sixiu Huang, Zheng Xu, Zhenfang Wu, Zicong Li
Summary: The quality of in vitro matured oocytes is usually lower than that of in vivo matured oocytes, possibly due to the absence of some important signal regulators in vitro. In this study, we found that progesterone and androstenedione were upregulated in the follicular fluid (FF) during in vivo pig oocyte maturation. The supplementation of progesterone or androstenedione during in vitro maturation significantly improved the pig oocyte maturation rate and subsequent embryo developmental competence.
Review
Agriculture, Dairy & Animal Science
Camila Bortoliero Costa, Trudee Fair, Marcelo M. Seneda
Summary: The oocyte is crucial for the development of life and plays a significant role in embryonic survival and successful pregnancy. This article focuses on key aspects of oocyte and follicle development in cattle, as well as recent advancements in reproductive biotechnologies centered around the oocyte and follicle.
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Cell Biology
Jing Wang, Zhiyong Zhuo, Xiao Ma, Yunjie Liu, Jing Xu, Changjiu He, Yao Fu, Feng Wang, Pengyun Ji, Lu Zhang, Guoshi Liu
Summary: The study found that melatonin protects against nuclear inhibition caused by hypoxanthine and restores oocyte meiosis via MT1 by reducing the intracellular concentration of cAMP.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Luba Nemerovsky, Hadas Bar-Joseph, Anat Eldar-Boock, Irit Miller, Ido Ben-Ami, Ruth Shalgi
Summary: Molecular changes caused by environmental factors affect oocyte quality and development potential, with oxidative stress playing a major role. Pigment epithelium-derived factor (PEDF) can mitigate the adverse effects of oxidative stress on oocytes, improving maturation rate and preserving mtDNA levels and ATP content.
Article
Biochemical Research Methods
Hanna J. McLennan, Avishkar Saini, Georgina M. Sylvia, Erik P. Schartner, Kylie R. Dunning, Malcolm S. Purdey, Tanya M. Monro, Andrew D. Abell, Jeremy G. Thompson
JOURNAL OF BIOPHOTONICS
(2020)
Article
Endocrinology & Metabolism
Madeleine J. Cox, Melissa C. Edwards, Valentina Rodriguez Paris, Ali Aflatounian, William L. Ledger, Robert B. Gilchrist, Vasantha Padmanabhan, David J. Handelsman, Kirsty A. Walters
Review
Obstetrics & Gynecology
Dulama Richani, Kylie R. Dunning, Jeremy G. Thompson, Robert B. Gilchrist
Summary: Metabolism is crucial for oocyte quality, with glucose being essential for COC maturation and energy production. Mitochondria are the main ATP source in oocytes, while cumulus cells play a vital role in maintaining ATP levels. New insights in oocyte lipid metabolism and innovative strategies in IVM have shown positive effects on oocyte competence.
HUMAN REPRODUCTION UPDATE
(2021)
Article
Endocrinology & Metabolism
Irene E. Sucquart, Ruchi Nagarkar, Melissa C. Edwards, Valentina Rodriguez Paris, Ali Aflatounian, Michael J. Bertoldo, Rebecca E. Campbell, Robert B. Gilchrist, Denovan P. Begg, David J. Handelsman, Vasantha Padmanabhan, Richard A. Anderson, Kirsty A. Walters
Summary: PCOS is a prevalent endocrine condition with a range of endocrine, reproductive, and metabolic abnormalities; Androgen actions play a key role in PCOS pathogenesis; NK3R antagonism may be beneficial in treating adverse metabolic features associated with PCOS and support the development of novel therapeutic strategies.
Article
Genetics & Heredity
Megan Lim, Hannah M. Brown, Ryan D. Rose, Jeremy G. Thompson, Kylie R. Dunning
Summary: Hemoglobin and 2,3-BPG may have a role in the maturation of cumulus-oocyte complexes (COCs). The study found that in vitro maturation (IVM) increases the expression of Bpgm within COCs compared to in vivo. Decreased oxygen concentration and the addition of hemoglobin affected Bpgm expression, but not to the levels observed in vivo.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Editorial Material
Genetics & Heredity
Michel De Vos, Michael Grynberg, Tuong M. Ho, Ye Yuan, David F. Albertini, Robert B. Gilchrist
Summary: This review aims to help professionals understand the appropriate use of IVM, supporting its clinical potential and safety, and exploring the current and future role of IVM in human ART.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Genetics & Heredity
Annie Whitty, Karen L. Kind, Kylie R. Dunning, Jeremy G. Thompson
Summary: This study found that under low oxygen conditions (7%), oocyte developmental competence is negatively impacted when glucose availability is limited. However, under low oxygen with high glucose concentration or adequate availability, developmental rates can be restored to levels seen in 20% oxygen groups. This highlights the importance of considering glucose concentration and physical culture conditions when comparing the effects of different oxygen concentrations during in vitro maturation.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Genetics & Heredity
Suliman H. Yagoub, Jeremy G. Thompson, Antony Orth, Kishan Dholakia, Brant C. Gibson, Kylie R. Dunning
Summary: This study designed and fabricated a micrometer scale device to simplify the intracytoplasmic sperm injection procedure, aiming to improve embryo production and reduce variability among operators. The device demonstrated successful embryo culture and microinjection capabilities without the need for a holding pipette, enhancing traceability and efficiency in the process.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Genetics & Heredity
Carl A. Campugan, Megan Lim, Darren J. X. Chow, Tiffany C. Y. Tan, Tong Li, Avishkar A. Saini, Antony Orth, Philipp Reineck, Erik P. Schartner, Jeremy G. Thompson, Kishan Dholakia, Kylie R. Dunning
Summary: Our study investigates the impact of different wavelengths of light on the development of embryos. We found that exposure to the yellow wavelength led to a significant reduction in the number of embryos developing to the blastocyst stage. Increased DNA damage was observed in embryos exposed to the blue, green, or red wavelengths. Only the red wavelength showed a significant decrease in total cell number. Additionally, pregnancy rates were significantly reduced and weight at weaning was significantly higher when embryos were exposed to the red and yellow wavelengths respectively. Our results highlight the wavelength-specific and energy-dependent effects of light on embryo development.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Genetics & Heredity
Suliman H. Yagoub, Megan Lim, Tiffany C. Y. Tan, Darren J. X. Chow, Kishan Dholakia, Brant C. Gibson, Jeremy G. Thompson, Kylie R. Dunning
Summary: A novel photopolymerized device is presented for housing and preserving oocytes and embryos during vitrification, simplifying the procedure and improving traceability.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Correction
Genetics & Heredity
Suliman H. Yagoub, Megan Lim, Tiffany C. Y. Tan, Darren J. X. Chow, Kishan Dholakia, Brant C. Gibson, Jeremy G. Thompson, Kylie R. Dunning
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Multidisciplinary Sciences
Hanna J. McLennan, Adam J. Blanch, Samuel J. Wallace, Lesley J. Ritter, Shauna L. Heinrich, David K. Gardner, Kylie R. Dunning, Marty J. Gauvin, Allison K. Love, Jeremy G. Thompson
Summary: This study introduces a microfluidic chip that is designed and completely 3D-printed using two-photon polymerization (2PP). It consists of ten channels and ten retrievable cell sample seats, which can enhance cell-surface interactions at the nanoscale and support cell culture. This technology provides the precision needed for nanoliter fluidic devices constructed from multiple interlocking parts.
SCIENTIFIC REPORTS
(2023)
Article
Obstetrics & Gynecology
Robert B. Gilchrist, Johan Smitz
Summary: Oocyte in vitro maturation (IVM) is a minimally invasive technique involving the collection and culture of immature oocytes to maturity outside the body. It is now classified as nonexperimental and is primarily indicated for patients with a high antral follicle count and for fertility preservation in cancer patients. Significant advances in IVM culture methods have been made based on animal studies and a simplified patient treatment protocol, leading to its routine practice in specialized centers worldwide.
FERTILITY AND STERILITY
(2023)
Article
Endocrinology & Metabolism
Valentina Rodriguez Paris, Melissa C. Edwards, Ali Aflatounian, Michael J. Bertoldo, William L. Ledger, David J. Handelsman, Robert B. Gilchrist, Kirsty A. Walters
Summary: PCOS is a common disorder with unclear etiology and pathogenesis, managed based on symptoms. A PCOS mouse model showed that irregular cycles, anovulation, and weight gain are early features, while adiposity, impaired glucose tolerance, and dyslipidemia are later characteristics. These findings support early diagnosis with body weight criteria.
JOURNAL OF THE ENDOCRINE SOCIETY
(2021)
