Article
Cell Biology
Long Jin, Hai-Ying Zhu, Xiang-Jin Kang, Li-Ping Lin, Pu-Yao Zhang, Tao Tan, Yang Yu, Yong Fan
Summary: Oxybenzone, a common UV filter in sunscreens and cosmetics, has been found to have negative effects on oocyte maturation, but melatonin shows protective effects against these detrimental effects.
Article
Cell Biology
Song-Hee Lee, Ming-Hong Sun, Dongjie Zhou, Wen-Jie Jiang, Xiao-Han Li, Geun Heo, Xiang-Shun Cui
Summary: Exposure to high temperature has been found to negatively affect the development of porcine oocytes. It induces mitochondrial dysfunction, ER stress, abnormal Golgi apparatus distribution, and lysosomal damage, leading to the failure of meiotic maturation in the oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Luba Nemerovsky, Hadas Bar-Joseph, Anat Eldar-Boock, Irit Miller, Ido Ben-Ami, Ruth Shalgi
Summary: Molecular changes caused by environmental factors affect oocyte quality and development potential, with oxidative stress playing a major role. Pigment epithelium-derived factor (PEDF) can mitigate the adverse effects of oxidative stress on oocytes, improving maturation rate and preserving mtDNA levels and ATP content.
Article
Agriculture, Dairy & Animal Science
Xiaoya Li, Jiaxin Duan, Shiyou Wang, Jianyong Cheng, Huali Chen, Zelin Zhang, Li Yang, Rongmao Hua, Qingwang Li
Summary: This study found that isorhamnetin can protect oocytes from damage caused by ZEA through the PI3K/Akt signaling pathway, improving developmental capability and inhibiting early apoptosis, oxidative stress, and endoplasmic reticulum stress.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Zongshuai Li, Yali Liu, Tian Ma, Chen Lv, Yina Li, Hongwei Duan, Xingxu Zhao, Jianlin Wang, Yong Zhang
Summary: Zearalenone (ZEN), a fungal toxin widely present in forage and food, poses a threat to animal and human reproductive health. This study using Smart-seq2 technology revealed that ZEN inhibits the in vitro maturation of ovine oocytes and reduces the proliferation of cumulus cells.
Article
Cell Biology
Ting Wang, Jie Na
Summary: The nucleolus undergoes significant functional changes during mammalian oocyte meiotic maturation, with FBL-GFP serving as a marker for nucleolus activity and correlating with transcription activity and oocyte quality. Different FBL-GFP patterns in oocytes can help identify those with superior meiotic maturation potential, with active nascent RNA transcription observed in NSN oocytes and transcriptionally quiescent state in SN oocytes. Global transcriptome profiling revealed significant differences in single SN and NSN oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hye Min Kim, Min Kook Kang, Se Yoon Seong, Jun Hyeon Jo, Min Ju Kim, Eun Kyeong Shin, Chang Geun Lee, Seung Jin Han
Summary: All eukaryotic cells, including oocytes, use cyclin-dependent kinase (Cdk) as an engine to drive the cell cycle. The key regulator of the oocyte cell cycle is the Cdk1-cyclin B1 complex. Recent studies have discovered the roles of other cyclins in mouse oocyte cell cycle regulation, as well as the translation and degradation of cyclins to modulate Cdk activity. This review provides new insights into the regulatory role and function of cyclins in oocyte cell cycle progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Z. Trebichalska, D. Kyjovska, S. Kloudova, P. Otevrel, A. Hampl, Z. Holubcova
Summary: Ultrastructural analysis of maturing human oocytes reveals complex changes in cytoplasmic architecture associated with the acquisition of egg's developmental competence. The study proposes a morphological map of cytoplasmic maturation, improving our understanding of human egg morphology, quality, and intracellular factors. This analysis provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Yumi Hoshino, Takafumi Uchida
Summary: Pin1, an enzyme that regulates the function of phosphorylated proteins, plays a crucial role in meiotic maturation. By altering the structure of proline peptide bond in proteins, Pin1 controls the progression of meiosis and affects the extrusion of polar bodies. The functional regulation of Pin1 is essential for oocyte production and holds potential applications in reproductive medicine and animal breeding.
Article
Cell Biology
Ying Chen, Chen Pan, Yajuan Lu, Yilong Miao, Bo Xiong
Summary: In this study, we found that HDAC8 plays a key role in regulating spindle assembly during porcine oocyte meiotic maturation. Depletion or inhibition of HDAC8 led to oocyte meiotic failure, including reduced polar body extrusion rate and aberrant spindle morphologies. The results suggest that HDAC8 is essential for proper chromosome alignment and spindle dynamics in female germ cell development.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Wen Gao, Chen Zhang, Bichun Li, Jeong Su Oh
Summary: Azoxystrobin (AZO), a widely used fungicide in agriculture, has been found to impair mouse oocyte maturation by disturbing spindle formation, chromosome alignment, mitochondrial distribution, and increasing oxidative stress. The toxicity of AZO to oocytes can be relieved by melatonin supplementation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Qi Jiang, Xin Qi, Chi Ding, Xingyu Liu, Yuanyuan Lei, Siying Li, Zubing Cao
Summary: Environmental pollutant dimethoate exposure impairs porcine oocyte maturation, but melatonin supplementation can restore meiotic maturation by suppressing the generation of reactive oxygen species, autophagy, and DNA damage accumulation. These findings suggest that melatonin could be a promising agent in improving the quality of dimethoate-exposed oocytes from humans and animals.
Article
Cell Biology
Di Wu
Summary: The germinal vesicle (GV) stage is a critical transition point in mammalian oocyte development that determines oocyte quality through active RNA degradation and transcriptome reprofiling. Oocyte RNA-seq has revealed differences between non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) phases. Single oocyte RNA-seq (soRNA-seq) shows higher reproducibility and a better illustration of gene changes during oocyte development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Ke Yan, Kexin Cui, Junyu Nie, Hengye Zhang, Lumin Sui, Huiting Zhang, Xiaogan Yang, Chang-Long Xu, Xingwei Liang
Summary: This study demonstrates that MV can protect oocytes from LPS-induced meiotic defects in part by reducing oxidative stress and maintaining m(6)A levels.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Lin Meng, Hongmei Hu, Zhiqiang Liu, Luyao Zhang, Qingrui Zhuan, Xue Li, Xiangwei Fu, Shien Zhu, Yunpeng Hou
Summary: Low calcium levels in oocytes can lead to delayed extrusion of the first polar body, impaired cytoplasmic maturation, and poor developmental phenotype of SCNT embryos. The study also found oxidative stress and apoptosis in low-calcium-model oocytes, with ID1 identified as a significant gene responsible for impaired reprogramming ability in oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.