Article
Oncology
Jie Zhou, Zengjie Lei, Jianfang Chen, Shengbo Liao, Yanrong Chen, Chengxiang Liu, Shuo Huang, Liuli Li, Yan Zhang, Pei Wang, Yinghui Huang, Jianjun Li, Houjie Liang
Summary: The study found that BATF2 is mainly localized in the cytoplasm rather than the nucleus of colorectal cancer (CRC) cells, and that cytoplasmic BATF2 expression is inversely correlated with CRC patient prognosis. The nuclear export and ubiquitin-mediated degradation of BATF2 in CRC cells were identified, revealing a functional nuclear export sequence (NES) in BATF2 through which it binds to CRM1 and translocates out of the nucleus, ultimately enhancing CRC growth via the AP-1/cyclin D1/pRb signaling pathway. Inhibiting nuclear export of BATF2 by mutating its NES or inhibiting CRM1 expression may be a promising therapeutic strategy for CRC.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Amrita Sule, Sarah E. Golding, Syed F. Ahmad, James Watson, Mostafa H. Ahmed, Glen E. Kellogg, Tytus Bernas, Sean Koebley, Jason C. Reed, Lawrence F. Povirk, Kristoffer Valerie
Summary: Ataxia telangiectasia mutated (ATM) is a key regulator of the DNA damage response (DDR) and plays a crucial role in the phosphorylation of the structural subunit A (PR65-S401) of protein phosphatase 2A (PP2A). Phosphorylation of PR65-S401 by ATM controls diverse cellular processes such as mitosis and cell growth, as well as the recovery from DNA damage. The study showed that mutations in S401 of PR65 resulted in chromosomal aberrations, impaired DNA double-strand break repair, and increased mitotic catastrophe after radiation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Mohamed Hamed, Birgit Caspar, Sarah A. Port, Ralph H. Kehlenbach
Summary: Nup214 is a major nucleoporin involved in nuclear protein and mRNA export processes, interacting with the export receptor CRM1 via specific FG repeats and a classic NES. Mutations in the NES of Nup214 lead to nuclear accumulation, suggesting a role in NPC biogenesis and CRM1-dependent protein export terminal steps. The rescue of mutant Nup214 nuclear export with artificial NES indicates a potential mechanism for correct localization of Nup88.
JOURNAL OF CELL SCIENCE
(2021)
Article
Oncology
Andrew B. Lassman, Patrick Y. Wen, Martin J. van den Bent, Scott R. Plotkin, Annemiek M. E. Walenkamp, Adam L. Green, Kai Li, Christopher J. Walker, Hua Chang, Sharon Tamir, Leah Henegar, Yao Shen, Mariano J. Alvarez, Andrea Califano, Yosef Landesman, Michael G. Kauffman, Sharon Shacham, Morten Mau-Sorensen
Summary: In patients with glioblastoma, single-agent Selinxor therapy achieved intratumoral penetration and demonstrated some efficacy with manageable side effects. Importantly, the dosage of Selinxor needs to be adjusted based on patient tolerability.
CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Moritz von Fallois, Friederike Katharina Kosyna, Markus Mandl, Yosef Landesman, Jurgen Dunst, Reinhard Depping
Summary: CRM1, as an overexpressed nuclear export receptor in cancer, can be selectively inhibited by Selinexor, a new FDA-approved drug. Selinexor significantly reduces the intranuclear level of HIF-1 alpha protein and enhances radiosensitivity in cancer cells, suggesting that targeting the HIF pathway by Selinexor could be a promising approach to overcome hypoxia-induced radioresistance.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Review
Oncology
Katherine L. B. Borden
Summary: This review highlights the importance of RNA export in cellular trafficking and the aberrant phenomena observed in cancer. Studies on RNA export machinery and relevant NPC components offer potential therapeutic strategies for targeting cancer.
Article
Hematology
Tiffany Tang, Peter Martin, Nagavalli Somasundaram, Cindy Lim, Miriam Tao, Eileen Poon, Maica J. D. Yunon, Shu Q. Toh, Sean X. Yan, Mohamad Farid, Jason Y. Chan, Soon T. Lim
Summary: The study investigated the safety and maximum tolerated dose of selinexor in combination with high-dose dexamethasone, ifosfamide, carboplatin, and etoposide in relapsed/refractory T-cell lymphoma and natural-killer/T-cell lymphoma. The combination therapy showed promising complete response rates in patients, but was poorly tolerated.
Article
Biochemistry & Molecular Biology
Sakalya Chavan, Deepak Khuperkar, Akshay Lonare, Swagatika Panigrahi, Jayesh Bellare, Srikanth Rapole, Vasudevan Seshadri, Jomon Joseph
Summary: The mechanism of small extracellular vesicle (sEV)-mediated intercellular communication is not yet clear. This study reveals that the key nucleo-cytoplasmic transport protein-RanGTPase is enriched in sEVs secreted by mammalian cells, and the recruitment of RanGTP into sEVs depends on the export receptor CRM1. The perturbation of nucleo-cytoplasmic transport affects the recruitment of cargo proteins into sEVs, suggesting a link between nucleo-cytoplasmic transport and sEV-mediated intercellular communication.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Xiaohu Lin, Qianqian Pang, Jie Hu, Jiaqi Sun, Siya Dai, Yijia Yu, Jinghong Xu
Summary: Sentrin/small ubiquitin-like modifier (SUMO) is a critical mediator in human diseases, including cancer and fibrosis. However, the mechanism of sumoylation in keloids is not well understood. We found that SUMO and RanGAP1 synergistically regulate keloids by modulating the nuclear export of Smad4. This study provides new insights into the mechanisms of keloids and nucleocytoplasmic transport.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Review
Endocrinology & Metabolism
Dan Du, Yugang Zhang, Canjun Zhu, Hong Chen, Jia Sun
Summary: The earliest function of hypoxia-inducible factor (HIF) was to respond to hypoxic conditions as a transcription factor. Recent studies have shown that HIF plays an essential role in central regulation of metabolism, affecting the central nervous system's response to glucose, inflammation, and hormonal influence on systemic metabolism. HIF primarily inhibits energy uptake and promotes energy expenditure in the hypothalamus, with its role depending on various factors and mechanisms within the hypothalamus.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bo Zhang, Yan Chen, Xiaolei Shi, Mi Zhou, Lei Bao, Kimmo J. Hatanpaa, Toral Patel, Ralph J. DeBerardinis, Yingfei Wang, Weibo Luo
Summary: This study reveals that hypoxia induces upregulation of the BCAA transporter LAT1 and the BCAA metabolic enzyme BCAT1 in human glioblastoma cells through the mediation of HIF-1 and HIF-2. HIF-1α plays a critical role in hypoxia-induced BCAT1 expression, and knockout of HIFs reduces glutamate labeling from BCAAs in GBM cells. Inhibition of BCAT1 inhibits GBM cell growth under hypoxia, highlighting a previously unrecognized HIF-dependent metabolic pathway.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Giorgia F. Camagni, Giovanni Minervini, Silvio C. E. Tosatto
Summary: The Prolyl Hydroxylases (PHDs) are a family of enzymes that regulate cell oxygen-sensing by hydroxylating hypoxia-inducible transcription factors alpha (HIFs-alpha) and promoting their degradation. Hypoxia inhibits PHDs activity, leading to HIFs-alpha stabilization and cell adaptation to low oxygen levels. The different isoforms of PHDs have varying effects on tumor progression. Molecular dynamics simulations, conservation analysis, and binding free energy calculations were used to study the binding properties and substrate affinity of PHD2 with HIF-1 alpha and HIF-2 alpha. The findings suggest that the PHD2 C-terminus may play a role in regulating PHD activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Liyan Cao, Fang Fu, Jianfei Chen, Hongyan Shi, Xin Zhang, Jianbo Liu, Da Shi, Yong Huang, Dewen Tong, Li Feng
Summary: Porcine parvovirus NS1 shuttles between the nucleus and cytoplasm via the CRM1-dependent nuclear export pathway and importin alpha/beta-mediated nuclear import pathway. The NESs and NLS of NS1 are essential for PPV replication. Blocking NS1's nuclear import or export inhibits PPV proliferation.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Medicine, Research & Experimental
Min Kyung Park, Jun Ji, Keeok Haam, Tae-Hee Han, Seona Lim, Mi-Jung Kang, Soon Sung Lim, Hyun Seung Ban
Summary: Licochalcone A, a component of Glycyrrhiza uralensis, demonstrates potential as a therapeutic agent in hypoxic cancer cells by inhibiting HIF-1 alpha accumulation and mitochondrial respiration, leading to increased oxygen content and suppression of cancer cell viability.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Cell Biology
Mariana Melani, Ayelen Valko, Nuria M. Romero, Milton O. Aguilera, Julieta M. Acevedo, Zambarlal Bhujabal, Joel Perez-Perri, Rocio V. de la Riva-Carrasco, Maximiliano J. Katz, Eleonora Sorianello, Cecilia D'Alessio, Gabor Juhasz, Terje Johansen, Maria I. Colombo, Pablo Wappner
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Biochemical Research Methods
Angela Maria Candreva, Mario Ferrer-Navarro, Silvia Bronsoms, Alejandra Quiroga, Renata Curciarello, Ana Cauerhff, Silvana Petruccelli, Guillermo Horacio Docena, Sebastian Alejandro Trejo
Article
Developmental Biology
MaryJane Shimell, Xueyang Pan, Francisco A. Martin, Arpan C. Ghosh, Pierre Leopold, Michael B. O'Connor, Nuria M. Romero
Review
Developmental Biology
Lautaro Gandara, Pablo Wappner
MECHANISMS OF DEVELOPMENT
(2018)
Correction
Biochemistry & Molecular Biology
Suzana Markolovic, Qinqin Zhuang, Sarah E. Wilkins, Charlotte D. Eaton, Martine I. Abboud, Maximiliano J. Katz, Helen E. McNeil, Robert K. Lesniak, Charlotte Hall, Weston B. Struwe, Rebecca Konietzny, Simon Davis, Ming Yang, Wei Ge, Justin L. P. Benesch, Benedikt M. Kessler, Peter J. Ratcliffe, Matthew E. Cockman, Roman Fischer, Pablo Wappner, Rasheduzzaman Chowdhury, Mathew L. Coleman, Christopher J. Schofield
NATURE CHEMICAL BIOLOGY
(2018)
Editorial Material
Developmental Biology
Juan R. Riesgo-Escovar, Roberto Mayor, Pablo Wappner, Irene Yan
MECHANISMS OF DEVELOPMENT
(2018)
Article
Biochemistry & Molecular Biology
Derya Deveci, Francisco A. Martin, Pierre Leopold, Nuria M. Romero
Review
Biochemistry & Molecular Biology
Renald Delanoue, Nuria M. Romero
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Multidisciplinary Sciences
L. Gandara, L. Durrieu, C. Behrensen, P. Wappner
SCIENTIFIC REPORTS
(2019)
Article
Cell Biology
Rocio de la Riva-Carrasco, Sebastian Perez-Pandolfo, Sofia Suarez Freire, Nuria M. Romero, Zambarlal Bhujabal, Terje Johansen, Pablo Wappner, Mariana Melani
Summary: Exocytosis is a fundamental physiological process for communication between cells, organs, and organisms, involving various proteins and regulators. The Drosophila transmembrane immunophilin Zonda (Zda) is a novel component of the exocytic machinery, highly expressed in secretory tissues and regulating exocytosis in multiple organs including the ring gland, insulin-producing cells, and salivary gland. The small GTPase RalA was identified as a crucial regulator of secretory granule exocytosis, required for fusion with the plasma membrane similar to Zda.
Review
Endocrinology & Metabolism
Celia G. Barredo, Beatriz Gil-Marti, Derya Deveci, Nuria M. Romero, Francisco A. Martin
Summary: Puberty and metamorphosis are two major developmental transitions associated with reproductive maturation, regulated by neuroendocrine pathways. These pathways play key roles not only in reproductive functions, but also in metabolism, sleep, and behavior. Some neuroendocrine circuits may have originated from a common bilaterian ancestor Urbilateria, leading to many conserved functions.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Applied
Angela Maria Candreva, Paola L. Smaldini, Ana Cauerhff, Silvana Petruccelli, Guillermo H. Docena
Summary: This study explored a preventive protocol for milk allergy with the oral administration of a soy-derived peptide containing cross-reactive epitopes, successfully preventing IgE-mediated milk sensitization through the induction of Th1-mediated immunity and induction of blocking IgG. This safe soy-peptide may represent a promising alternative for preventing milk allergy.
Article
Cell Biology
Ayelen Valko, Sebastian Perez-Pandolfo, Eleonora Sorianello, Andreas Brech, Pablo Wappner, Mariana Melani
Summary: Hypoxia induces autophagy in Drosophila melanogaster, which is essential for adaptation and survival. The process involves a bona fide autophagic response with waves of autophagosome formation and maturation, and is induced cell autonomously in different tissues. Autophagy under hypoxic conditions can be studied using D. melanogaster as a model organism, offering insights into its role in hypoxia-associated pathologies and developmentally regulated processes.
Article
Biochemistry & Molecular Biology
Milton Osmar Aguilera, Esteban Robledo, Mariana Melani, Pablo Wappner, Maria Isabel Colombo
Summary: In this article, the protein FKBP38 is reported to be a novel modulator of the initial steps of the autophagic pathway, specifically in starvation-induced autophagy. FKBP38 interacts with the VPS34 lipid kinase complex, with the transmembrane domain of FKBP38 being critical for its biological function.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Developmental Biology
Pablo Wappner, Mario Zurita
Summary: Despite being a new society, the Latin American Society for Developmental Biology (LASDB) has had a significant impact on the advancement of Developmental Biology in Latin America and globally since its establishment. By organizing congresses, courses, and fostering collaborations with prominent societies, LASDB has successfully promoted Developmental Biology beyond Latin America. Now, LASDB looks towards the future to continue supporting scientific endeavors in the region and around the world.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)