Article
Biochemistry & Molecular Biology
Xueqiao Wang, Zhuying Wei, Mingjuan Gu, Lin Zhu, Chao Hai, Anqi Di, Di Wu, Chunling Bai, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li
Summary: Myostatin (MSTN) is a negative regulator of skeletal muscle growth and plays an important role in regulating mitochondrial energy metabolism in mice. Loss of MSTN inhibits oxidative phosphorylation and impairs ATP production in skeletal muscles. This inhibition may be mediated through the TGF-beta-Smad2/3 pathway. These findings demonstrate that MSTN is a crucial regulator of mitochondrial energy homeostasis in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Gemma Gaitskell-Phillips, Francisco E. Martin-Cano, Jose M. Ortiz-Rodriguez, Antonio Silva-Rodriguez, Eva da Silva-Alvarez, Patricia Rojo-Dominguez, Jose A. Tapia, Maria C. Gil, Cristina Ortega-Ferrusola, Fernando J. Pena
Summary: Even in stallions with normal sperm quality at ejaculation, there are subtle differences that can affect sperm conservation and fertility. Proteomic studies on stallion sperm have revealed differences in protein composition related to motility and velocity, providing insights into the molecular basis of sperm function. These findings may offer a molecular explanation for the link between sperm motility and fertility in stallions.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Youjun Zhang, Jonas Giese, Sandra M. Kerbler, Beata Siemiatkowska, Leonardo Perez de Souza, Jessica Alpers, David Barbosa Medeiros, Dirk K. Hincha, Danilo M. Daloso, Mark Stitt, Iris Finkemeier, Alisdair R. Fernie
Summary: This study identified the roles of two mitochondrial phosphatases, Sal2 and PP2c63, along with pyruvate dehydrogenase kinase (PDK), in regulating the tricarboxylic acid (TCA) cycle. PDK specifically regulates the pyruvate dehydrogenase complex (PDC), while PP2c63 nonspecifically regulates PDC. The study also showed that these phosphatases affect flux through the TCA cycle and altered metabolism compromises plant growth in mutant lines.
Article
Biochemistry & Molecular Biology
Nils Rugen, Frank Schaarschmidt, Juergen Eirich, Iris Finkemeier, Hans-Peter Braun, Holger Eubel
Summary: Mitochondrial biology relies on the presence and activity of large protein assemblies involved in respiration, TCA cycle, glycine oxidation, and oxidative phosphorylation. This study investigates changes in composition and molecular mass of protein complexes in response to light, revealing dynamic associations between matrix enzymes and components involved in oxidative phosphorylation, laying the groundwork for future research on protein interactions regulating plant mitochondrial functions.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Review
Cell Biology
Trang T. T. Nguyen, Enyuan Shang, Mike-Andrew Westhoff, Georg Karpel-Massler, Markus D. Siegelin
Summary: Glioblastoma, the most common primary brain tumor in adults, displays reprogrammed metabolism and utilizes various fuel sources. Blocking glycolysis may lead to the reactivation of beta-oxidation of fatty acids.
Review
Oncology
Meixi Peng, Yongxiu Huang, Ling Zhang, Xueya Zhao, Yu Hou
Summary: Acute myeloid leukemia (AML) is a hematologic malignancy with poor prognosis, characterized by various cytogenetic and molecular abnormalities. Current treatments often fail to eliminate leukemic stem cells (LSCs), which exhibit a unique metabolic profile dependent on oxidative phosphorylation (OXPHOS). Understanding the regulation of OXPHOS in LSCs may lead to effective clinical therapies in AML.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biology
Dylan Gerard Ryan, Ming Yang, Hiran A. Prag, Giovanny Rodriguez Blanco, Efterpi Nikitopoulou, Marc Segarra-Mondejar, Christopher A. Powell, Tim Young, Nils Burger, Jan Lj Miljkovic, Michal Minczuk, Michael P. Murphy, Alex von Kriegsheim, Christian Frezza
Summary: This study comprehensively evaluated the consequences of TCA cycle inhibition in murine kidney epithelial cells, revealing a rewiring of redox and amino acid metabolism dependent on ATF4 and ISR activation. Additionally, a divergent metabolic response was uncovered, where acute FHi but not SDHi could maintain asparagine levels through reductive carboxylation and cytosolic aspartate synthesis. The interplay between the TCA cycle, redox biology, and amino acid homeostasis was highlighted in this work.
Review
Biochemistry & Molecular Biology
Woojin Kang, Miki Suzuki, Takako Saito, Kenji Miyado
Summary: The TCA cycle serves as the main source of cellular energy and its dysfunction related to human diseases, such as neurometabolic disorders and tumors, has attracted increasing interest. The distinct diseases resulting from loss or dysfunction of TCA cycle-related enzymes suggest each enzyme has a unique function. This review aims to provide a comprehensive overview of the relationship between each TCA cycle-related enzyme and human diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Alexis Paulina Jimenez-Uribe, Estefani Yaquelin Hernandez-Cruz, Karla Jaqueline Ramirez-Magana, Jose Pedraza-Chaverri
Summary: Mitochondria play important roles in cell functions, especially in kidney function. Besides energy production, mitochondria also communicate with the rest of the cell through various pathways and participate in multiple metabolic processes, including the TCA cycle.
Article
Food Science & Technology
Sulaiman K. Matarneh, Con-Ning Yen, Jocelyn Bodmer, Samer W. El-Kadi, David E. Gerrard
Summary: This study aimed to test mitochondrial functionality under conditions simulating postmortem metabolism using an in vitro model. The results showed that mitochondria can mobilize pyruvate generated through glycolysis in conditions simulating muscle postmortem metabolism.
Article
Biochemistry & Molecular Biology
Sanjit K. Dhar, Timothy Scott, Chi Wang, Teresa W. M. Fan, Daret K. St Clair
Summary: This study reveals the regulatory role of mitochondrial-generated superoxide radicals in gene expression and establishes a new paradigm that recognizes O2· as an initiator of metabolic reprogramming and epigenetic regulation in response to mitochondrial dysfunction.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Cell Biology
Mohamed Zakaria Nassef, Jasmin E. Hanke, Karsten Hiller
Summary: Mitochondria are crucial for cellular energy production and immune response, especially in macrophages where rapid adaptation to metabolic conditions is essential for neutralizing pathogens. Mitochondrial dysfunction in macrophages can lead to a dampened immune response.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Oncology
Reema Singh, Ian G. Mills
Summary: Prostate cancer is a high-incidence cancer linked to increased mitochondrial metabolic activity. Genomic drivers such as the androgen receptor, c-Myc, PTEN, and p53 are associated with metabolic dysregulation and progression of prostate cancer.
FRONTIERS IN ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Vittoria Infantino, Anna Santarsiero, Paolo Convertini, Simona Todisco, Vito Iacobazzi
Summary: In cancer cells, metabolic reprogramming under low-oxygen conditions is crucial for tumor survival, with HIF-1 playing a pivotal role. Mitochondria are key players in regulating cellular energy and closely interact with HIF-1 to drive metabolic and functional changes in cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Sven Dennerlein, Sabine Poerschke, Silke Oeljeklaus, Cong Wang, Ricarda Richter-Dennerlein, Johannes Sattmann, Diana Bauermeister, Elisa Hanitsch, Stefan Stoldt, Thomas Langer, Stefan Jakobs, Bettina Warscheid, Peter Rehling
Summary: TMEM223 and SMIM4 were identified as ribosome-associated proteins involved in complex IV and III biogenesis, respectively. They stimulate mRNA translation and support early assembly steps, linking translation machinery to the biogenesis of OXPHOS complexes.
