Genome engineering for improved recombinant protein expression in Escherichia coli

High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage

(2014) Junjie Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coli and preliminary purification process

(2014) Guillermo Marini et al.   BMC BIOTECHNOLOGY

Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering

(2014) Yan-Yan Cui et al.   Microbial Cell Factories

The dawn of evolutionary genome engineering

(2014) Csaba Pál et al.   NATURE REVIEWS GENETICS

Constraint-based models predict metabolic and associated cellular functions

(2014) Aarash Bordbar et al.   NATURE REVIEWS GENETICS

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

A Simple and Effective Method for Construction of Escherichia coli Strains Proficient for Genome Engineering

(2014) Young Shin Ryu et al.   PLoS One

Design and Optimization of Short DNA Sequences That Can Be Used as 5′ Fusion Partners for High-Level Expression of Heterologous Genes in Escherichia coli

(2013) Veronika Kucharova et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A novel strategy for enhancing extracellular secretion of recombinant proteins in Escherichia coli

(2013) Lingqia Su et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Genome-scale genetic engineering in Escherichia coli

(2013) Jaehwan Jeong et al.   BIOTECHNOLOGY ADVANCES

Dynamic transcriptional response ofEscherichia colito inclusion body formation

(2013) Faraz Baig et al.   BIOTECHNOLOGY AND BIOENGINEERING

Efficient export of prefolded, disulfide-bonded recombinant proteins to the periplasm by the Tat pathway in Escherichia coli CyDisCo strains

(2013) Cristina F. R. O. Matos et al.   BIOTECHNOLOGY PROGRESS

Cofactor engineering for advancing chemical biotechnology

(2013) Yipeng Wang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Genome-wide analysis of redox reactions reveals metabolic engineering targets for d-lactate overproduction in Escherichia coli

(2013) Hyun Ju Kim et al.   METABOLIC ENGINEERING

A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

(2013) Simone Balzer et al.   Microbial Cell Factories

Basic and applied uses of genome-scale metabolic network reconstructions of Escherichia coli

(2013) D. McCloskey et al.   Molecular Systems Biology

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

(2013) Dokyun Na et al.   NATURE BIOTECHNOLOGY

Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli

(2013) Seung Min Yoo et al.   Nature Protocols

Translational sensitivity of the Escherichia coli genome to fluctuating tRNA availability

(2013) Sibylle E. Wohlgemuth et al.   NUCLEIC ACIDS RESEARCH

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

(2013) Catherine Burke et al.   PLoS One

A Comparative Analysis of Industrial Escherichia coli K–12 and B Strains in High-Glucose Batch Cultivations on Process-, Transcriptome- and Proteome Level

(2013) Karoline Marisch et al.   PLoS One

Genomically Recoded Organisms Expand Biological Functions

(2013) M. J. Lajoie et al.   SCIENCE

COFACTOR MODIFICATION ANALYSIS: A COMPUTATIONAL FRAMEWORK TO IDENTIFY COFACTOR SPECIFICITY ENGINEERING TARGETS FOR STRAIN IMPROVEMENT

(2013) MEIYAPPAN LAKSHMANAN et al.   Journal of Bioinformatics and Computational Biology

Non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in rE. coli fed-batch cultures

(2013) Adilson da Silva et al.   SpringerPlus

The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology

(2012) Sofia J. Costa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High-yield export of a native heterologous protein to the periplasm by the tat translocation pathway in Escherichia coli

(2012) Cristina F.R.O. Matos et al.   BIOTECHNOLOGY AND BIOENGINEERING

Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions

(2012) Jeffrey D Orth et al.   BMC Systems Biology

Enhancing toxic protein expression in Escherichia coli fed-batch culture using kinetic parameters: Human granulocyte-macrophage colony-stimulating factor as a model system

(2012) Yogender Pal Khasa et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Metabolic responses to recombinant bioprocesses in Escherichia coli

(2012) Sónia Carneiro et al.   JOURNAL OF BIOTECHNOLOGY

An inverse metabolic engineering approach for the design of an improved host platform for over-expression of recombinant proteins in Escherichia coli

(2012) Chaitali Ghosh et al.   Microbial Cell Factories

Strong stimulation of recombinant protein production in Escherichia coli by combining stimulatory control elements in an expression cassette

(2012) Friederike Zwick et al.   Microbial Cell Factories

The new pLAI (lux regulon based auto-inducible) expression system for recombinant protein production in Escherichia coli

(2012) Salvatore Nocadello et al.   Microbial Cell Factories

Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)

(2012) Lingqia Su et al.   Microbial Cell Factories

SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

(2012) Julie Lobstein et al.   Microbial Cell Factories

Supplementation of Substrate Uptake Gene Enhances the Expression of rhIFN-β in High Cell Density Fed-Batch Cultures of Escherichia coli

(2012) Anuradha B. Singh et al.   MOLECULAR BIOTECHNOLOGY

Advances in shaking technologies

(2012) Wolf Klöckner et al.   TRENDS IN BIOTECHNOLOGY

A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli

(2012) Martin Dragosits et al.   Journal of Biological Engineering

Optimization of Human Granulocyte Macrophage-Colony Stimulating Factor (hGM-CSF) Expression Using Asparaginase and Xylanase Gene’s Signal Sequences in Escherichia coli

(2011) Yogender Pal Khasa et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

An engineered Escherichia coli having a high intracellular level of ATP and enhanced recombinant protein production

(2011) Hye-Jung Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Self-cleaving fusion tags for recombinant protein production

(2011) Yifeng Li   BIOTECHNOLOGY LETTERS

Ribosome degradation in growing bacteria

(2011) Kerli Piir et al.   EMBO REPORTS

Quantification of metabolic limitations during recombinant protein production in Escherichia coli

(2011) Jan Heyland et al.   JOURNAL OF BIOTECHNOLOGY

Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineering

(2011) Hendrik Waegeman et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Manipulating respiratory levels in Escherichia coli for aerobic formation of reduced chemical products

(2011) Jiangfeng Zhu et al.   METABOLIC ENGINEERING

Analyzing the metabolic stress response of recombinant Escherichia coli cultures expressing human interferon-beta in high cell density fed batch cultures using time course transcriptomic data

(2011) Anuradha B. Singh et al.   Molecular BioSystems

Increasing recombinant protein production in Escherichia coli K12 through metabolic engineering

(2011) Hendrik Waegeman et al.   New Biotechnology

Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli

(2011) Brent L. Nannenga et al.   PROTEIN SCIENCE

Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement

(2011) Farren J. Isaacs et al.   SCIENCE

Genome-Scale Analysis of Translation Elongation with a Ribosome Flow Model

(2011) Shlomi Reuveni et al.   PLoS Computational Biology

Comparative transcriptomic profile analysis of fed-batch cultures expressing different recombinant proteins in Escherichia coli

(2011) Ashish K Sharma et al.   AMB Express

Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains

(2010) Y. J. Choi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Growth and recombinant protein expression with Escherichia coli in different batch cultivation media

(2010) Ralf Hortsch et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A review of advanced small-scale parallel bioreactor technology for accelerated process development: Current state and future need

(2010) Rachel Bareither et al.   BIOTECHNOLOGY PROGRESS

Promoter knock-in: a novel rational method for the fine tuning of genes

(2010) Marjan De Mey et al.   BMC BIOTECHNOLOGY

OptORF: Optimal metabolic and regulatory perturbations for metabolic engineering of microbial strains

(2010) Joonhoon Kim et al.   BMC Systems Biology

Measuring growth rate in high-throughput growth phenotyping

(2010) Anders Blomberg   CURRENT OPINION IN BIOTECHNOLOGY

Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation

(2010) Baley A Fong et al.   Microbial Cell Factories

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

(2010) Joseph R Warner et al.   NATURE BIOTECHNOLOGY

What is flux balance analysis?

(2010) Jeffrey D Orth et al.   NATURE BIOTECHNOLOGY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system

(2010) Jonghyeon Shin et al.   Journal of Biological Engineering

Extracellular recombinant protein production from Escherichia coli

(2009) Ye Ni et al.   BIOTECHNOLOGY LETTERS

Techniques for oxygen transfer measurement in bioreactors: a review

(2009) S Suresh et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Kinetic studies of recombinant human interferon-gamma expression in continuous cultures of E. coli

(2009) S. Thangminlal Vaiphei et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering

(2009) Joseph A. Chemler et al.   METABOLIC ENGINEERING

Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD)

(2009) Chan Chung et al.   Microbial Cell Factories

Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

(2009) Frank Kensy et al.   Microbial Cell Factories

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

(2009) Frank Kensy et al.   Microbial Cell Factories

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

Increased Hydrogen Production by Genetic Engineering of Escherichia coli

(2009) Zhanmin Fan et al.   PLoS One

Design Parameters to Control Synthetic Gene Expression in Escherichia coli

(2009) Mark Welch et al.   PLoS One

The expression of recombinant genes inEscherichia colican be strongly stimulated at the transcript production level by mutating the DNA-region corresponding to the 5′-untranslated part of mRNA

(2009) Laila Berg et al.   Microbial Biotechnology

Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli

(2009) Stéphanie Bury-Moné et al.   PLoS Genetics

Integrating metabolic, transcriptional regulatory and signal transduction models in Escherichia coli

(2008) Markus W. Covert et al.   BIOINFORMATICS

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

(2008) Nicola A. Burgess-Brown et al.   PROTEIN EXPRESSION AND PURIFICATION

Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners

(2008) Seonghun Kim et al.   PROTEIN EXPRESSION AND PURIFICATION

Single-step affinity purification of recombinant proteins using a self-excising module fromNeisseria meningitidisFrpC

(2008) Lenka Sadilkova et al.   PROTEIN SCIENCE

A pause for thought along the co-translational folding pathway

(2008) Anton A. Komar   TRENDS IN BIOCHEMICAL SCIENCES

Cellular response to accumulation of recombinant proteins in the E. coli inner membrane: Implications for proteolysis and productivity of the secretory expression system

(2007) Balaji Balagurunathan et al.   BIOCHEMICAL ENGINEERING JOURNAL

Redistribution of Metabolic Fluxes in the Central Aerobic Metabolic Pathway of E. coli Mutant Strains with Deletion of the ackA-pta and poxB Pathways for the Synthesis of Isoamyl Acetate

(2005) Cheryl R. Dittrich et al.   BIOTECHNOLOGY PROGRESS