Nanobodies and their potential applications

A bacterial-two-hybrid selection system for one-step isolation of intracellularly functional Nanobodies

(2012) Mireille Pellis et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Advances in bispecific biotherapeutics for the treatment of cancer

(2012) Chad May et al.   BIOCHEMICAL PHARMACOLOGY

Pharmacokinetic, Biodistribution, and Biophysical Profiles of TNF Nanobodies Conjugated to Linear or Branched Poly(ethylene glycol)

(2012) Yulia Vugmeyster et al.   BIOCONJUGATE CHEMISTRY

Nanobody-Based Targeting of the Macrophage Mannose Receptor for EffectiveIn VivoImaging of Tumor-Associated Macrophages

(2012) Kiavash Movahedi et al.   CANCER RESEARCH

Nanobody-induced perturbation of LFA-1/L-plastin phosphorylation impairs MTOC docking, immune synapse formation and T cell activation

(2012) Sarah De Clercq et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Nanobodies Targeting Mouse/Human VCAM1 for the Nuclear Imaging of Atherosclerotic Lesions

(2012) Alexis Broisat et al.   CIRCULATION RESEARCH

Clinical Experience with Gene Therapy and Bispecific Antibodies for T Cell-based Therapy of Cancer

(2012) Patrick A. Baeuerle et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Cell-penetrating anti-GFAP VHH and corresponding fluorescent fusion protein VHH-GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging

(2012) Tengfei Li et al.   FASEB JOURNAL

Identification of broadly protective human antibodies toPseudomonas aeruginosaexopolysaccharide Psl by phenotypic screening

(2012) Antonio DiGiandomenico et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Fusion to a highly charged proteasomal retargeting sequence increases soluble cytoplasmic expression and efficacy of diverse anti-synuclein intrabodies

(2012) Shubhada N. Joshi et al.   mAbs

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

Pre-clinical studies of toxin-specific Nanobodies: Evidence of in vivo efficacy to prevent fatal disturbances provoked by scorpion envenoming

(2012) Issam Hmila et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Chaperonin GroEL a Brucella immunodominant antigen identified using Nanobody and MALDI-TOF-MS technologies

(2012) A.Q. Abbady et al.   VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY

Specific Cell Targeting with Nanobody Conjugated Branched Gold Nanoparticles for Photothermal Therapy

(2011) Bieke Van de Broek et al.   ACS Nano

Magnetosome Expression of Functional Camelid Antibody Fragments (Nanobodies) in Magnetospirillum gryphiswaldense

(2011) Anna Pollithy et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Antithrombotic drug candidate ALX-0081 shows superior preclinical efficacy and safety compared with currently marketed antiplatelet drugs

(2011) H. Ulrichts et al.   BLOOD

Using microdialysis to analyse the passage of monovalent nanobodies through the blood-brain barrier

(2011) G Caljon et al.   BRITISH JOURNAL OF PHARMACOLOGY

Protein transduction domain delivery of therapeutic macromolecules

(2011) Arjen van den Berg et al.   CURRENT OPINION IN BIOTECHNOLOGY

Immuno-imaging using nanobodies

(2011) Ilse Vaneycken et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering antibodies and proteins for molecular in vivo imaging

(2011) Tina Romer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Strategies for extended serum half-life of protein therapeutics

(2011) Roland E Kontermann   CURRENT OPINION IN BIOTECHNOLOGY

Targeting epidermal growth factor receptor in tumors: From conventional monoclonal antibodies via heavy chain-only antibodies to nanobodies

(2011) Isil Altintas et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Nanobody; an Old Concept and New Vehicle for Immunotargeting

(2011) Fatemeh Rahbarizadeh et al.   IMMUNOLOGICAL INVESTIGATIONS

A biparatopic anti-EGFR nanobody efficiently inhibits solid tumour growth

(2011) Rob C. Roovers et al.   INTERNATIONAL JOURNAL OF CANCER

Nanobody — Shell functionalized thermosensitive core-crosslinked polymeric micelles for active drug targeting

(2011) Marina Talelli et al.   JOURNAL OF CONTROLLED RELEASE

Nanobody-coupled microbubbles as novel molecular tracer

(2011) Sophie Hernot et al.   JOURNAL OF CONTROLLED RELEASE

Research strategies to improve snakebite treatment: Challenges and progress

(2011) Robert A. Harrison et al.   Journal of Proteomics

Oriented conjugates of single-domain antibodies and quantum dots: toward a new generation of ultrasmall diagnostic nanoprobes

(2011) Alyona Sukhanova et al.   Nanomedicine-Nanotechnology Biology and Medicine

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

Fluorescent fusion protein knockout mediated by anti-GFP nanobody

(2011) Emmanuel Caussinus et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic  2-microglobulin variant

(2011) K. Domanska et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Case Of Convergence: Why Did a Simple Alternative to Canonical Antibodies Arise in Sharks and Camels?

(2011) Martin F. Flajnik et al.   PLOS BIOLOGY

High Affinity Nanobodies against the Trypanosome brucei VSG Are Potent Trypanolytic Agents that Block Endocytosis

(2011) Benoît Stijlemans et al.   PLoS Pathogens

Kinetic analysis of novel mono- and multivalent VHH-fragments and their application for molecular imaging of brain tumours

(2010) U Iqbal et al.   BRITISH JOURNAL OF PHARMACOLOGY

A bispecific nanobody to provide full protection against lethal scorpion envenoming

(2010) Issam Hmila et al.   FASEB JOURNAL

Single Domain Intracellular Antibodies from Diverse Libraries

(2010) Tomoyuki Tanaka et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bispecific antibodies for cancer therapy

(2010) Patrick Chames et al.   mAbs

Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells

(2010) Sai T Reddy et al.   NATURE BIOTECHNOLOGY

By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selection

(2010) U. Ravn et al.   NUCLEIC ACIDS RESEARCH

Direct Injection of Functional Single-Domain Antibodies from E. coli into Human Cells

(2010) Ana Blanco-Toribio et al.   PLoS One

CXCR4 nanobodies (VHH-based single variable domains) potently inhibit chemotaxis and HIV-1 replication and mobilize stem cells

(2010) S. Jahnichen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The culprit behind amyloid beta peptide related neurotoxicity in Alzheimer's disease: oligomer size or conformation?

(2010) Kerensa Broersen et al.   Alzheimers Research & Therapy

Specific immuno capturing of the Staphylococcal superantigen toxic-shock syndrome toxin-1 in plasma

(2009) Hendrik Adams et al.   BIOTECHNOLOGY AND BIOENGINEERING

Bispecific T-Cell Engaging Antibodies for Cancer Therapy

(2009) P. A. Baeuerle et al.   CANCER RESEARCH

Engineering of recombinant crystallization chaperones

(2009) Shohei Koide   CURRENT OPINION IN STRUCTURAL BIOLOGY

Anti-MUC1 Nanobody Can Redirect T-Body Cytotoxic Effector Function

(2009) Seyed Hamid Aghaee Bakhtiari et al.   HYBRIDOMA

Biotinylated Bionanocapsules for Displaying Diverse Ligands Toward Cell-specific Delivery

(2009) T. Shishido et al.   JOURNAL OF BIOCHEMISTRY

Production, characterization and in vitro testing of HBcAg-specific VHH intrabodies

(2009) B. Serruys et al.   JOURNAL OF GENERAL VIROLOGY

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

(2009) Janusz Wesolowski et al.   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins

(2009) B. R. McNaughton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Single-domain antibodies as building blocks for novel therapeutics

(2008) D SAERENS et al.   CURRENT OPINION IN PHARMACOLOGY

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Selection and characterization of KDEL-specific VHH antibody fragments and their application in the study of ER resident protein expression

(2008) Rinse Klooster et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Improved tumor targeting of anti-epidermal growth factor receptor Nanobodies through albumin binding: taking advantage of modular Nanobody technology

(2008) B. M. Tijink et al.   MOLECULAR CANCER THERAPEUTICS

Single-domain antibodies recognize selectively small oligomeric forms of amyloid β, prevent Aβ-induced neurotoxicity and inhibit fibril formation

(2008) Pierre Lafaye et al.   MOLECULAR IMMUNOLOGY

Toward chaperone-assisted crystallography: Protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (VHH) scaffold

(2008) Valentina Tereshko et al.   PROTEIN SCIENCE