Article
Biochemistry & Molecular Biology
Can Balaban, Martin Sztacho, Ludovica Antiga, Ana Miladinovic, Masahiko Harata, Pavel Hozak
Summary: In this study, Myosin Phosphatase Rho-Interacting Protein (MPRIP) was identified as a novel regulator of RNA polymerase II (RNAPII) transcription. MPRIP recruits Tyr1-phosphorylated CTD (Tyr1P-CTD) to nuclear PIP2-containing structures, affecting transcription and condensation. These findings shed light on the role of PIP2 in RNAPII transcription.
Article
Biology
Jingtao Li, Xiaoying Ma, Chenyang Wang, Sihui Liu, Gang Yu, Mingming Gao, Hengwei Qian, Mengjie Liu, Ben F. Luisi, Dean W. Gabriel, Wenxing Liang
Summary: The effector Fol-Secreted Virulence-related Protein1 (FolSvp1) of fungal pathogen Fusarium oxysporum f. sp. lycopersici (Fol) regulates pathogenicity by binding and translocating the tomato pathogenesis-related protein1 (SlPR1) via acetylation, leading to the abolishment of defense signaling and facilitation of pathogen invasion.
Article
Oncology
Jyoti Kashyap, Rakesh K. Tyagi
Summary: Mitosis is a crucial process for cell self-renewal, and recent studies have shown that some transcription factors remain attached to chromatin during cell division, ensuring the reactivation of necessary transcription factors. This study confirms Vitamin D Receptor (VDR) as a mitotic bookmarking factor that plays a crucial role in maintaining cell identity and genome bookmarking.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Ivana Malcova, Ladislav Bumba, Filip Uljanic, Darya Kuzmenko, Jana Nedomova, Jana Kamanova
Summary: Research has shown that the BteA effector protein binds to negatively charged membrane phospholipids through its lipid raft targeting domain. The binding constants for different lipids were determined, and it was found that phosphatidylinositol 4,5-bisphosphate, phosphatidic acid, and phosphatidylserine were required for targeting the BteA effector to the plasma membrane of yeast cells. Additionally, electrostatic and hydrophobic interactions were involved in the membrane association of the effector protein.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Cell Biology
Juane Lu, Tao Wu, Biao Zhang, Suke Liu, Wenjun Song, Jianjun Qiao, Haihua Ruan
Summary: This article summarizes the types of nuclear localization signals, focuses on recently reported proteins containing such signals, and briefly outlines mechanisms for nuclear entry that do not rely on nuclear localization signals.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Multidisciplinary Sciences
Jarred M. Whitlock, Evgenia Leikina, Kamran Melikov, Luis Fernandez De Castro, Sandy Mattijssen, Richard J. Maraia, Michael T. Collins, Leonid V. Chernomordik
Summary: In this study, it was found that the nuclear RNA chaperone La protein plays a role in regulating osteoclast fusion. The levels of La protein decrease during monocyte-to-osteoclast differentiation but reappear as a low molecular weight form at the surface of fusing osteoclasts, promoting fusion. The fusion-promoting role of La protein is independent of its canonical interactions with RNA and involves interactions with Annexin A5.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Guo-Qing Chen, Pei Li, Qing Yan, Yi-Hong Wu, Hao-Ran Wang, Shu-Fen Chao, Li-Juan Wu, Long Chen, Guo-Zhong Feng
Summary: Proteins with nuclear localization signals are transported into the nucleus through the importin-alpha/beta-mediated pathway. Importin-alpha proteins have been mainly studied in Drosophila, with little knowledge about them in Lepidoptera insects. This study identified four putative importin-alpha homologues in Sf9 cells, showing different nuclear localization patterns and specific interactions with AcMNPV DNA polymerase.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
So Woon Kim, Mi Kyung Kim, Seokwoo Hong, Areum Choi, Jee Hye Choi, Shmuel Muallem, Insuk So, Dongki Yang, Ana Maria Cuervo, Hyun Jin Kim
Summary: This study reveals that lipid-regulated TRPML3 is the Ca2+ release channel in the phagophore, providing the necessary Ca2+ for autophagy progress. Phosphatidylinositol-3-phosphate (PI3P) is a selective regulator of TRPML3, and its interaction with TRPML3 activates the channel and promotes autophagy. These findings demonstrate that TRPML3 is a key regulator of autophagy and PI3P is crucial for its activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Luis Adrian De Jesus-Gonzalez, Selvin Noe Palacios-Rapalo, Jose Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, Carlos Noe Farfan-Morales, Carlos Daniel Cordero-Rivera, Bulmaro Cisneros, Ana Lorena Gutierrez-Escolano, Rosa Maria del Angel
Summary: Recent evidence suggests that nuclear elements play a role in the replication of Flaviviruses, with certain viral proteins being imported into the nucleus. Furthermore, nuclear localization of these proteins has been observed in infected cells. The presence of ZIKV NS3 in the nucleus has been investigated, showing that NS3 is imported into the nucleus via the importin pathway and exported back to the cytoplasm.
JOURNAL OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jonas Loetscher, Adria-Arnau Marti Lindez, Nicole Kirchhammer, Elisabetta Cribioli, Greta Maria Paola Giordano Attianese, Marcel P. Trefny, Markus Lenz, Sacha Rothschild, Paolo Strati, Marco Kuenzli, Claudia Lotter, Susanne H. Schenk, Philippe Dehio, Jordan Loeliger, Ludivine Litzler, David Schreiner, Victoria Koch, Nicolas Page, Dahye Lee, Jasmin Graehlert, Dmitry Kuzmin, Anne-Valerie Burgener, Doron Merkler, Miklos Pless, Maria L. Balmer, Walter Reith, Joerg Huwyler, Melita Irving, Carolyn G. King, Alfred Zippelius, Christoph Hess
Summary: This study reveals the importance of extracellular magnesium in cellular immunity, particularly in the activation of the co-stimulatory molecule LFA-1. The findings demonstrate that magnesium-sufficiency enhances the function of immune cells and the effectiveness of T cell therapies. Low serum magnesium levels are associated with disease progression and reduced overall survival in patients receiving CAR T cell and immune checkpoint antibody treatments.
Article
Biochemistry & Molecular Biology
Ivy Y. W. Chung, Lei Li, Oleg Tyurin, Alla Gagarinova, Raissa Wibawa, Pengfei Li, Elizabeth L. Hartland, Miroslaw Cygler
Summary: Legionella pneumophila is an intracellular pathogen that causes Legionnaire's disease in humans. It enters host cells through phagocytosis and affects host cellular processes by secreting over 300 effector proteins. RavA, a L. pneumophila effector, displays unique structural characteristics and localizes to the Golgi apparatus and plasma membrane in human cells.
Article
Microbiology
Lei Song, Yongchao Xie, Chuang Li, Lidong Wang, Chunlin He, Yong Zhang, Jingya Yuan, Jingjing Luo, Xi Liu, Yu Xiu, Hang Li, Marina Gritsenko, Ernesto S. Nakayasu, Yue Feng, Zhao-Qing Luo
Summary: The study reveals that SdjA functions as a dual enzyme, inhibiting the activity of SdeB and SdeC by glutamylation and acting as a deglutamylase to reverse SidJ-induced glutamylation on SdeA. This dual function of SdjA highlights the unique regulation of phosphoribosyl ubiquitination by the SidJ effector family.
Article
Multidisciplinary Sciences
Xiao Li, D. Eric Anderson, Yuen-Yan Chang, Michal Jarnik, Matthias P. Machner
Summary: Legionella pneumophila controls vacuole expansion by fine-tuning the generation of lysophospholipids within the vacuolar membrane. Overproduction of VpdC prevents the adequate expansion of the surrounding membrane, trapping the replicating bacteria within spatially confined vacuoles and reducing their capability to proliferate intracellularly.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Computer Science, Artificial Intelligence
Yige Zhang, Weixiong Rao, Kun Zhang, Lei Chen
Summary: In recent years, a large amount of data generated by telecommunication cellular networks has been used for outdoor mobile device localization and transportation mode detection. However, existing solutions cannot handle the heterogeneous mobility patterns of mobile devices. Therefore, this paper proposes a multi-task learning-based deep neural network framework, PRNet(+), which outperforms other methods according to extensive evaluation.
IEEE TRANSACTIONS ON KNOWLEDGE AND DATA ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Kumi Sakoe, Norifumi Shioda, Tohru Matsuura
Summary: Spastin is a microtubule-severing AAA ATPase that plays important roles in cell division and neurogenesis, with mutations in the SPAST gene leading to neurodegenerative disorders. The two main isoforms of Spastin, M1 and M87, differ in their N-terminal amino acids and have alternative splicing variants. A newly identified NES region containing exon4 regulates the subcellular localization of Spastin in coordination with NLS controlled by phosphorylation state.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Oncology
Saeed S. Akhand, Hao Chen, Stephen Connor Purdy, Zian Liu, Joshua C. Anderson, Christopher D. Willey, Michael K. Wendt
Summary: T-DM1 efficiently delivers microtubule inhibitor to HER2 overexpressing cells, while induction of EMT may promote emergence of T-DM1 resistant cells.
Article
Oncology
Mackenzie L. Davenport, John B. Echols, Austin D. Silva, Joshua C. Anderson, Philip Owens, Clayton Yates, Qing Wei, Shuko Harada, Douglas R. Hurst, Mick D. Edmonds
Summary: miR-31 demonstrates oncogenic properties in specific subtypes of lung cancer, highlighting its potential as a therapeutic target in these subtypes.
Article
Medicine, General & Internal
J. Robert Wong, G. Robert Gish, Ramsey Cheung, S. Amit Chitnis
Summary: The prevalence of vaccination or documented reactivity to hepatitis A antibody and hepatitis B surface antibody among US adults with chronic liver disease is alarmingly low. This is particularly concerning in individuals with nonalcoholic fatty liver disease and alcoholic liver disease.
AMERICAN JOURNAL OF MEDICINE
(2021)
Letter
Gastroenterology & Hepatology
Chari Cohen, Kate Moraras, Michalea Jackson, Maureen Kamischke, Robert G. Gish, Carol L. Brosgart, Mehlika Toy, David Hutton, Timothy M. Block, Su Wang, Samuel So
Article
Oncology
Roni Shouval, Ana Alarcon Tomas, Joshua A. Fein, Jessica R. Flynn, Ettai Markovits, Shimrit Mayer, Aishat Olaide Afuye, Anna Alperovich, Theodora Anagnostou, Michal J. Besser, Connie Lee Batlevi, Parastoo B. Dahi, Sean M. Devlin, Warren B. Fingrut, Sergio A. Giralt, Richard J. Lin, Gal Markel, Gilles Salles, Craig S. Sauter, Michael Scordo, Gunjan L. Shah, Nishi Shah, Ruth Scherz-Shouval, Marcel van den Brink, Miguel-Angel Perales, Maria Lia Palomba
Summary: TP53 mutations/CNAs are important determinants of response and survival in LBCL patients treated with CD19-CAR-T therapy. TP53 alterations are associated with poor treatment outcomes and lower survival rates.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Review
Gastroenterology & Hepatology
Yasser Fouad, Jean-Francois Dufour, Ming-Hua Zheng, Steven Bollipo, Hailemichael Desalegn, Henning Gronbaek, Robert G. Gish
Summary: There is a polarizing debate in hepatology regarding the name and redefinition of fatty liver disease associated with metabolic dysfunction. While there is growing evidence supporting the use of the term metabolic (dysfunction) associated fatty liver disease (MAFLD) for clinical and academic practice, controversy remains. This opinion piece argues that understanding scientific consensus and exploring alternative models for reaching agreement are crucial in this field.
LIVER INTERNATIONAL
(2022)
Article
Physiology
Helen E. Collins, Joshua C. Anderson, Adam R. Wende, John C. Chatham
Summary: This study discovered that STIM1 regulates the activity of calcium-dependent kinases and phosphatases in adult mouse hearts. This has important implications for understanding the role of STIM1 in cardiac physiology and pathophysiology.
PHYSIOLOGICAL REPORTS
(2022)
Letter
Gastroenterology & Hepatology
Salah Kivlighn, Eric Schuur, Robert G. Gish, Donna Cryer
Article
Immunology
Robert J. Wong, Harvey W. Kaufman, Justin K. Niles, Hema Kapoor, Robert G. Gish
Summary: Current treatment eligibility algorithms for chronic hepatitis B are complex and may contribute to missed opportunities for early antiviral therapy. Using a large US laboratory database, we propose a stepwise simplification of chronic hepatitis B treatment eligibility to be considered, aiming to improve timely treatment.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Gastroenterology & Hepatology
Nicolette Veracruz, Robert G. Gish, Ramsey Cheung, Amit S. Chitnis, Robert J. Wong
Summary: The impact of hepatitis B virus (HBV) and hepatitis C virus (HCV) on disability-adjusted life years (DALYs) has shown significant disparities globally. From 2010 to 2019, there has been an overall decrease in DALYs, but differences in DALY improvements across world regions suggest unequal access to viral hepatitis care and treatment.
LIVER INTERNATIONAL
(2022)
Review
Cell Biology
Yanina Bogliotti, Mark Vander Roest, Aras N. Mattis, Robert G. Gish, Gary Peltz, Robin Anwyl, Salah Kivlighn, Eric R. Schuur
Summary: Liver disease is a major cause of global mortality, with late-stage liver disease being characterized by hepatocyte injury, inflammation, and aberrant tissue healing. Treatment options for liver disease are limited, but cell replacement therapy using hepatocyte-like cells shows promise as a safe and effective treatment option.
Article
Medicine, Research & Experimental
Christian T. Stackhouse, Joshua C. Anderson, Zongliang Yue, Thanh Nguyen, Nicholas J. Eustace, Catherine P. Langford, Jelai Wang, James R. Rowland, Chuan Xing, Fady M. Mikhail, Xiangqin Cui, Hasan Alrefai, Ryan E. Bash, Kevin J. Lee, Eddy S. Yang, Anita B. Hjelmeland, C. Ryan Miller, Jake Y. Chen, Yancey Gillespie, Christopher D. Willey
Summary: The key molecular regulators of acquired radiation resistance in recurrent glioblastoma (GBM) are largely unknown. In this study, patient-derived xenograft (PDX) models were generated to mimic the longitudinal evolution of patient recurrent tumors following radiation therapy. Whole-exome sequencing and bioinformatics analysis identified genomic alterations and kinomic alterations associated with acquired radiation resistance. The study also found multiple molecular routes to acquired radiation resistance and identified targetable kinases for potential therapeutic interventions. This research provides important insights into overcoming treatment resistance in GBM patients.
Article
Gastroenterology & Hepatology
David Lam, Robert J. Wong, Adla Tessier, Yenice Zapata, Elsie Saldana, Robert G. Gish
Summary: This study aimed to explore the impact of demographic-based and insurance-based barriers on HCV treatment access for the FQHC population. It found that insurance restrictions were the greatest challenge affecting treatment outcomes in the study population.
GASTROENTEROLOGY RESEARCH
(2022)
Article
Oncology
David Levitz, Yogen Saunthararajah, Kateryna Fedorov, Lauren C. Shapiro, Ioannis Mantzaris, Aditi Shastri, Noah Kornblum, R. Alejandro Sica, Nishi Shah, Marina Konopleva, Kira Gritsman, Ira Braunschweig, Dennis L. Cooper, Kith Pradhan, Amit Verma, Eric J. Feldman, Mendel Gold Finger
Summary: The once-weekly dosing regimen of LDDec and VEN in AML and MDS patients shows promising activity, with high response rates and extended time on therapy compared to standard dosing HMA/VEN regimens, highlighting the importance of noncytotoxic DNA methyltransferase 1-targeting.
CLINICAL CANCER RESEARCH
(2023)
Article
Oncology
Jiani Chai, Jui Choudhuri, Qing Wang, Yanan Fang, Yang Shi, Josette Kamel, Nishi Shah, R. Alejandro Sica, Noah Kornblum, Marina Konopleva, Ioannis Mantzaris, Aditi Shastri, Kira Gritsman, Amit Verma, Mendel Goldfinger, Swati Goel, Yanhua Wang, Xuejun Tian
Summary: Although PD-L1 overexpression is observed in solid tumors, its expression patterns in acute myeloid leukemia (AML) are not well studied. This study shows that PD-L1 expression is significantly upregulated in AML cases with activating mutations in JAK2/STATs compared to wildtype controls. There is a positive correlation between phosphorylated STAT3 expression and PD-L1 expression. The CPS scoring system could be used to quantitatively measure PD-L1 expression in leukemias and JAK2/STATs mutant AML could be potential candidates for checkpoint inhibitor trials.
LEUKEMIA & LYMPHOMA
(2023)