Review
Cell Biology
Dan Wang, Haichun Ma, Yutong Zhao, Jing Zhao
Summary: USP14 is a ubiquitin-specific protease associated with the proteasome and plays crucial roles in cellular functions, viral infection, inflammatory responses, neurodegenerative diseases, and tumorigenesis. It appears to have a dual function in regulating intracellular proteolysis, inhibiting degradation of ubiquitinated proteins while also promoting protein degradation via proteasome activation. Additionally, USP14 is involved in the regulation of autophagy, suggesting a key role in intracellular proteolytic pathways.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun-Hao Wen, Xiang-Hong He, Ze-Sen Feng, Dong-Yi Li, Ji-Xin Tang, Hua-Feng Liu
Summary: The accumulation of protein aggregates is a characteristic feature of neurodegenerative diseases. Protein homeostasis imbalance caused by proteotoxic stress or mutant proteins leads to protein aggregation, interfering with cellular processes and promoting the progression of age-related neurodegenerative diseases. Cells have evolved mechanisms to rescue or eliminate protein aggregates, and potential therapeutic strategies targeting protein aggregates are being explored.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yan Zhu, Lukman O. Afolabi, Xiaochun Wan, Joong Sup Shim, Liang Chen
Summary: Neurodegenerative diseases are characterized by the degeneration of the central or peripheral nervous systems and the aggregation of misfolded proteins, leading to cellular dysfunction and brain damage. TRIM proteins play important roles in maintaining protein quality control and clearing misfolded protein aggregates associated with neurodegenerative diseases.
Review
Agriculture, Multidisciplinary
Hanieh Nargeh, Fatemeh Aliabadi, Marjan Ajami, Hamidreza Pazoki-Toroudi
Summary: Neurodegenerative diseases are a significant public health challenge due to their association with aging. Understanding the molecular and cellular mechanisms involved in their pathogenesis is crucial. Protein aggregations resulting from UPS inefficiency and gut microbiota alternations are key pathogenic features, with polyphenols potentially playing a role in decreasing disease development rates. The conversion of dietary polyphenols into different microbial metabolites with diverse biological properties highlights the need for thorough investigation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Neurosciences
Jenny Lozada Ortiz, Marina Betancor, Sonia Perez Lazaro, Rosa Bolea, Juan J. J. Badiola, Alicia Otero
Summary: Chronic accumulation of misfolded proteins can cause endoplasmic reticulum stress, triggering the unfolded protein response. In this study, the expression of three endoplasmic reticulum stress markers, PERK, BiP, and PDI, were investigated in sheep affected by scrapie. The results showed significantly higher levels of these markers in scrapie-infected sheep compared to healthy controls. In addition, increased accumulation of ubiquitin aggregates was observed in the brains of scrapie-affected animals, suggesting impairment of the ubiquitin-proteasome system in natural scrapie. These findings suggest that these proteins may serve as biomarkers and therapeutic targets for prion diseases.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Cekala, Karolina Trepczyk, Daria Sowik, Przemyslaw Karpowicz, Artur Gieldon, Julia Witkowska, Malgorzata Gizynska, Elzbieta Jankowska, Ewa Wieczerzak
Summary: In this study, we designed and obtained a series of peptidomimetic stimulators of 20S proteasome, which can enhance protein degradation and increase the activity of proteasome in cells. These compounds may have the potential to counteract proteasome impairment in patients with age-related diseases.
Article
Biotechnology & Applied Microbiology
Filipp Bezold, Johannes Scheffer, Philipp Wendering, Zahra Razaghi-Moghadam, Jonathan Trauth, Bastian Pook, Hagen Nusshaer, Sophia Hasenjaeger, Zoran Nikoloski, Lars-Oliver Essen, Christof Taxis
Summary: Dynamic metabolic engineering is a strategy to switch key metabolic pathways in microbial cell factories to increase production of valuable chemicals. In this study, optogenetic intervention was used to arrest the cell cycle of budding yeast, resulting in increased production of terpenoid and nucleoside analog. Proteomic analysis revealed significant changes in metabolic key enzymes, and integration of the data in metabolic models demonstrated modulation of fluxes associated with product synthesis. These results highlight the potential of optogenetically triggered cell cycle intervention in improving compound yields in cellular factories.
METABOLIC ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Min Qiu, Jimei Chen, Xiaohong Li, Jian Zhuang
Summary: Cardiovascular diseases (CVDs) are a major social problem worldwide, and oxidative stress plays a vital role in CVDs. The ubiquitin-proteasome system (UPS) is associated with oxidative stress and affects the development of CVDs. This review provides a comprehensive analysis of the roles of UPS and oxidative stress in CVDs, suggesting new targets for the treatment and prevention of CVDs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Xiaoguang Liu, Charbel Moussa
Summary: USP-13 is a de-ubiquitinase that reverses protein degradation by cleaving ubiquitin off protein substrates. It plays a role in neurodegenerative diseases by regulating protein clearance through ubiquitination and de-ubiquitination. Additionally, USP-13 also affects the process of protein clearance.
Review
Cell Biology
Michela Damizia, Ludovica Altieri, Patrizia Lavia
Summary: Nuclear import receptors play crucial regulatory roles in various cellular processes such as mitosis, ciliogenesis, and phase separation. Their expression levels have significant impacts on cellular functions and disease development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Applied
Luigia Di Stasio, Leonardo Sciammaro, Salvatore De Caro, Maria Victoria Salinas, Maria Cecilia Puppo, Gianfranco Mamone
Summary: In this study, the proteome composition of pistachio seeds was investigated using various proteomics analyses. The results identified proteins belonging to the cupin protein family and allergens, and also compared the proteomes of different pistachio varieties.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Review
Cell Biology
Anita V. Kumar, Joslyn Mills, Louis R. Lapierre
Summary: Efficient proteostasis is crucial for cellular maintenance, and the protein p62 plays a key role as a selective autophagy receptor and also participates in other cellular processes. p62 has context-dependent impacts on aging and diseases, reflecting its involvement in maintaining proteostasis. This review highlights recent advances in understanding p62's functions and mechanisms and its implications on aging and neurodegeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Paula Moyano, Emma Sola, Maria Victoria Naval, Lucia Guerra-Menendez, Maria De la Cabeza Fernandez, Javier del Pino
Summary: The amount and diversity of environmental pollutants (EPs) have increased due to human activities, and several neurodegenerative diseases (NDs) are believed to be related to EPs exposure. EPs induce neurotoxic effects, including the accumulation of misfolded toxic proteins, leading to neuronal dysfunction and cell death. Heat shock proteins (HSPs) and the proteasome system are involved in the elimination of these toxic proteins, but exposure to EPs can disrupt their function. Therapeutic approaches involving recombinant HSPs/proteasome or compounds that increase their expression or activity have been proposed. This article reviews the current literature on NDs related to EP exposure and their relationship with proteasome system and HSPs dysfunction, as well as discusses the toxic effects and contradictory findings. It also covers the therapeutic use of drugs and recombinant proteasome/HSPs to eliminate toxic proteins and prevent/treat EP-induced neurodegeneration.
Article
Cell Biology
Oygul Mirzalieva, Meredith Juncker, Joshua Schwartzenburg, Shyamal Desai
Summary: ISG15 is an interferon-associated protein that plays a dual role in various human diseases. Its expression is aberrantly elevated or compromised in diseases such as cancer, neurodegenerative disorders, and TBI. ISG15 and ISGylation are now considered as diagnostic/prognostic biomarkers and therapeutic targets due to their roles in cancer cell proliferation, migration, and metastasis, as well as drug sensitivity.
Article
Biochemistry & Molecular Biology
Raymond T. Anderson, Thomas A. Bradley, David M. Smith
Summary: This study constructed an animal model of Caenorhabditis elegans that expressed a hyperactive, open gate proteasome, resulting in significantly increased lifespan, enhanced resistance to oxidative and proteotoxic stress, and decreased fertility. The findings suggest that targeting the proteasome gating mechanism may be a valid approach for future research on age-related diseases in mammals.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Alida Spalloni, Silvia Caioli, Elisa Bonomi, Cristina Zona, Emanuele Buratti, Antonella Alberici, Barbara Borroni, Patrizia Longone
Summary: FTD-CSF affects the cortical and spinal cord cultures differently, showing toxic effects on certain types of neurons such as GABA+ and calbindin-D28k+ neurons.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Valentina Vacca, Sara Marinelli, Federica De Angelis, Daniela F. Angelini, Eleonora Piras, Luca Battistini, Flaminia Pavone, Roberto Coccurello
Summary: This study identified gender differences in immune responses to neuropathic pain, with females showing higher infiltration of T cells in nerves and males showing higher infiltration of macrophages. Additionally, cytokine and chemokine levels were differently regulated in blood and nerve lysates from female mice compared to male mice. These findings provide insights into gender-associated inflammatory profiles in neuropathy and could contribute to the development of differential biomarkers and personalized medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Correction
Immunology
Daniela Francesca Angelini, Federica De Angelis, Valentina Vacca, Eleonora Piras, Chiara Parisi, Michele Nutini, Alida Spalloni, Francesca Pagano, Patrizia Longone, Luca Battistini, Flaminia Pavone, Sara Marinelli
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Simona D'Aguanno
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Rossella Luca, Maria Rita Assenza, Fabio Maiullari, Luisa Pieroni, Silvia Maiullari, Giulia Federici, Federica Marini, Roberto Rizzi, Andrea Urbani, Silvia Soddu, Fabiola Moretti
Summary: MDM4 impairs the metastatic process of EOC by inhibiting mTOR activity, reducing cell migration. High levels of MDM4 expression are associated with significantly increased 15-year survival rate and negative impact on the efficacy of mTOR inhibitors.
CELL DEATH & DISEASE
(2021)
Article
Pharmacology & Pharmacy
Silvia Scaricamazza, Illari Salvatori, Susanna Amadio, Valentina Nesci, Alessio Torcinaro, Giacomo Giacovazzo, Aniello Primiano, Michela Gloriani, Niccolo Candelise, Luisa Pieroni, Jean-Philippe Loeffler, Frederique Rene, Cyril Quessada, Tesfaye W. Tefera, Hao Wang, Frederik J. Steyn, Shyuan T. Ngo, Gabriella Dobrowolny, Elisa Lepore, Andrea Urbani, Antonio Musaro, Cinzia Volonte, Elisabetta Ferraro, Roberto Coccurello, Cristiana Valle, Alberto Ferri
Summary: The therapeutic potential of the multi-target drug trimetazidine was evaluated in SOD1(G93A) mice. The results showed that trimetazidine delayed disease progression, improved motor function and metabolism, and increased overall survival of the mice.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Valerio Caputo, Giulia Calvino, Claudia Strafella, Andrea Termine, Carlo Fabrizio, Giulia Trastulli, Arcangela Ingrasci, Cristina Peconi, Silvia Bardini, Angelo Rossini, Antonino Salvia, Giovanna Borsellino, Luca Battistini, Carlo Caltagirone, Raffaella Cascella, Emiliano Giardina
Summary: This study demonstrated the utility of a diagnostic kit for tracking the Omicron variant in Italy by testing COVID-19 samples. The results showed that the Omicron variant spread more rapidly compared to the Alpha and Delta variants. The use of this diagnostic kit could reduce the time and cost of monitoring strategies.
Article
Chemistry, Analytical
Mozghan Boroumand, Barbara Manconi, Simone Serrao, Federica Iavarone, Alessandra Olianas, Tiziana Cabras, Cristina Contini, Luisa Pieroni, Maria Teresa Sanna, Giovanni Vento, Chiara Tirone, Claudia Desiderio, Antonella Fiorita, Gavino Faa, Irene Messana, Massimo Castagnola
Summary: Glutathionylation and cysteinylation can protect critical cysteines from oxidative damages. Levels of S100A9 long and cystatin B in saliva were determined in preterm and at-term newborns, showing that the percentage of S-thiolated derivatives increased with postconceptional age, with the most significant variation occurring around 280 days. Differences in S-thiolated derivatives levels were found to be dependent on postconceptional age, not on preterm or at-term birth.
SEPARATION SCIENCE PLUS
(2022)
Article
Oncology
Cristina Banella, Gianfranco Catalano, Serena Travaglini, Elvira Pelosi, Tiziana Ottone, Alessandra Zaza, Gisella Guerrera, Daniela Francesca Angelini, Pasquale Niscola, Mariadomenica Divona, Luca Battistini, Maria Screnci, Emanuele Ammatuna, Ugo Testa, Clara Nervi, Maria Teresa Voso, Nelida Ines Noguera
Summary: Acute Myeloid Leukemias (AMLs) are rapidly progressing and clonal neoplastic diseases. In this study, the metabolic background of AML cells was characterized, and the combination of ascorbate and buformin was shown to be a potential innovative therapeutic option for elderly AML patients resistant to traditional therapy.
Article
Cell Biology
Christian Barbato, Paola Frisone, Laura Braccini, Simona D'Aguanno, Luisa Pieroni, Maria Teresa Ciotti, Caterina Catalanotto, Carlo Cogoni, Francesca Ruberti
Summary: RNA-binding proteins play important roles in miRNA-mediated gene silencing. This study discovered that SERBP1 is a partner protein of Argonaute2, and it participates in miR-92-mediated gene silencing by regulating the 3' UTR of KCC2. These findings advance our understanding of KCC2 translational regulation in neuronal cells and highlight the key role of SERBP1 in this gene regulation.
Article
Cell Biology
Patrizia Bottoni, Alessandro Pontoglio, Salvatore Scara, Luisa Pieroni, Andrea Urbani, Roberto Scatena
Summary: Mitochondrial bioenergetics play an important role in pathophysiology, especially as unintended targets of different drugs. PPAR ligands, a class of drugs that activate Peroxisome Proliferator-Activated Receptors, were found to inhibit NADH coenzyme Q reductase and induce complex mitochondrial oxidative metabolism damage. This damage affects not only ATP synthesis but also respiratory control ratio, spare respiratory capacity, coupling efficiency, oxidative stress, and mitochondrial structural damage.
Article
Clinical Neurology
Sara Cappelli, Alida Spalloni, Fabian Feiguin, Giulia Visani, Ursa Susnjar, Anna-Leigh Brown, Marco De Bardi, Giovanna Borsellino, Maria Secrier, Hemali Phatnani, Maurizio Romano, Pietro Fratta, Patrizia Longone, Emanuele Buratti
Summary: This study demonstrates a co-regulation relationship between Nitric Oxide Synthase 1 Adaptor Protein and TAR DNA-binding protein 43 kDa in related diseases, suggesting it as a potential therapeutic target.
BRAIN COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Elisabetta Valentini, Simona D'Aguanno, Marta Di Martile, Camilla Montesano, Virginia Ferraresi, Alexandros Patsilinakos, Manuela Sabatino, Lorenzo Antonini, Martina Chiacchiarini, Sergio Valente, Antonello Mai, Gianni Colotti, Rino Ragno, Daniela Trisciuoglio, Donatella Del Bufalo
Summary: Bcl-2 family anti-apoptotic proteins play a significant role in cancer, and finding new molecules targeting these proteins has therapeutic potential. Through virtual screening and experimental validation, IS20 and IS21 were identified as compounds that can inhibit the activity of Bcl-2 family proteins and have anti-proliferative and pro-apoptotic effects on various tumors.
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)