Heparin and related polysaccharides: synthesis using recombinant enzymes and metabolic engineering

Analysis of Total Human Urinary Glycosaminoglycan Disaccharides by Liquid Chromatography–Tandem Mass Spectrometry

(2015) Xiaojun Sun et al.   ANALYTICAL CHEMISTRY

High Cell Density Cultivation of Recombinant Escherichia coli Strains Expressing 2-O-Sulfotransferase and C5-Epimerase for the Production of Bioengineered Heparin

(2015) Jianhua Zhang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Combinatorial one-pot chemoenzymatic synthesis of heparin

(2015) Ujjwal Bhaskar et al.   CARBOHYDRATE POLYMERS

Metabolic pathway balancing and its role in the production of biofuels and chemicals

(2015) J Andrew Jones et al.   CURRENT OPINION IN BIOTECHNOLOGY

Structural and Functional Study of d-Glucuronyl C5-epimerase

(2015) Yi Qin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Production of chondroitin in metabolically engineered E. coli

(2015) Wenqin He et al.   METABOLIC ENGINEERING

Heparin stability by determining unsubstituted amino groups using hydrophilic interaction chromatography mass spectrometry

(2014) Li Fu et al.   ANALYTICAL BIOCHEMISTRY

High cell density cultivation of a recombinantEscherichia colistrain expressing a 6-O-sulfotransferase for the production of bioengineered heparin

(2014) J. Zhang et al.   JOURNAL OF APPLIED MICROBIOLOGY

Molecular Mechanism of Substrate Specificity for Heparan Sulfate 2-O-Sulfotransferase

(2014) Chunhui Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and Activity of a New Low-Molecular-Weight Heparin Produced by Enzymatic Ultrafiltration

(2014) Li Fu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Homogeneous low-molecular-weight heparins with reversible anticoagulant activity

(2014) Yongmei Xu et al.   Nature Chemical Biology

Assays for determining heparan sulfate and heparin O-sulfotransferase activity and specificity

(2013) Eric Sterner et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Characterization of currently marketed heparin products: composition analysis by 2D-NMR

(2013) David A. Keire et al.   Analytical Methods

Tailored Design and Synthesis of Heparan Sulfate Oligosaccharide Analogues Using Sequential One-Pot Multienzyme Systems

(2013) Yi Chen et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Expression of Low Endotoxin 3-O-Sulfotransferase in Bacillus subtilis and Bacillus megaterium

(2013) Wenya Wang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production

(2013) Odile Francesca Restaino et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biosynthesis and function of chondroitin sulfate

(2013) Tadahisa Mikami et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Infliximab in Psoriasis and Psoriatic Arthritis

(2013) Marina Papoutsaki et al.   BIODRUGS

An 'omics approach towards CHO cell engineering

(2013) Payel Datta et al.   BIOTECHNOLOGY AND BIOENGINEERING

Transcriptional engineering ofEscherichia coliK4 for fructosylated chondroitin production

(2013) Qiulin Wu et al.   BIOTECHNOLOGY PROGRESS

Pathway and protein engineering approaches to produce novel and commodity small molecules

(2013) Namita Bhan et al.   CURRENT OPINION IN BIOTECHNOLOGY

The heparanase/syndecan-1 axis in cancer: mechanisms and therapies

(2013) Vishnu C. Ramani et al.   FEBS Journal

Biological functions of iduronic acid in chondroitin/dermatan sulfate

(2013) Martin A. Thelin et al.   FEBS Journal

Chemoenzymatic synthesis of glycosaminoglycans: Re-creating, re-modeling and re-designing nature's longest or most complex carbohydrate chains

(2013) P. L. DeAngelis et al.   GLYCOBIOLOGY

Bioengineered Chinese Hamster Ovary Cells with Golgi-targeted 3-O-Sulfotransferase-1 Biosynthesize Heparan Sulfate with an Antithrombin-binding Site

(2013) Payel Datta et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Immobilized enzymes to convert N-sulfo, N-acetyl heparosan to a critical intermediate in the production of bioengineered heparin

(2013) Jian Xiong et al.   JOURNAL OF BIOTECHNOLOGY

Structural Characterization of Pharmaceutical Heparins Prepared from Different Animal Tissues

(2013) Li Fu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Homologous overexpression of rfaH in E. coli K4 improves the production of chondroitin-like capsular polysaccharide

(2013) Donatella Cimini et al.   Microbial Cell Factories

Top-Down Approach for the Direct Characterization of Low Molecular Weight Heparins Using LC-FT-MS

(2012) Lingyun Li et al.   ANALYTICAL CHEMISTRY

Monosaccharide precursors for boosting chondroitin-like capsular polysaccharide production

(2012) Odile Francesca Restaino et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering plant metabolism into microbes: from systems biology to synthetic biology

(2012) Peng Xu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Synthetic heparin

(2012) Robert J Linhardt et al.   CURRENT OPINION IN PHARMACOLOGY

Uncovering Biphasic Catalytic Mode of C5-epimerase in Heparan Sulfate Biosynthesis

(2012) Juzheng Sheng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Construction of a Chondroitin Sulfate Library with Defined Structures and Analysis of Molecular Interactions

(2012) Nobuo Sugiura et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Metabolic engineering of Chinese hamster ovary cells: Towards a bioengineered heparin

(2012) Jong Youn Baik et al.   METABOLIC ENGINEERING

Metabolic engineering of Escherichia coli BL21 for biosynthesis of heparosan, a bioengineered heparin precursor

(2012) Chunyu Zhang et al.   METABOLIC ENGINEERING

Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin

(2012) A. F. Moon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural characterization of heparins from different commercial sources

(2011) Fuming Zhang et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Synthesis of heparosan oligosaccharides by Pasteurella multocida PmHS2 single-action transferases

(2011) Anaïs A. E. Chavaroche et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Control of the heparosan N-deacetylation leads to an improved bioengineered heparin

(2011) Zhenyu Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering of routes to heparin and related polysaccharides

(2011) Ujjwal Bhaskar et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Photochemical preparation of a novel low molecular weight heparin

(2011) Kyohei Higashi et al.   CARBOHYDRATE POLYMERS

Expression of heparan sulfate sulfotransferases in Kluyveromyces lactis and preparation of 3'-phosphoadenosine-5'-phosphosulfate

(2011) X. Zhou et al.   GLYCOBIOLOGY

The Dominating Role ofN-Deacetylase/N-Sulfotransferase 1 in Forming Domain Structures in Heparan Sulfate

(2011) Juzheng Sheng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disaccharide analysis of glycosaminoglycan mixtures by ultra-high-performance liquid chromatography–mass spectrometry

(2011) Bo Yang et al.   JOURNAL OF CHROMATOGRAPHY A

Chemoenzymatic Synthesis of Uridine Diphosphate-GlcNAc and Uridine Diphosphate-GalNAc Analogs for the Preparation of Unnatural Glycosaminoglycans

(2011) Sayaka Masuko et al.   JOURNAL OF ORGANIC CHEMISTRY

Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli

(2011) Van Nguyen et al.   Microbial Cell Factories

Chemoenzymatic Synthesis of Homogeneous Ultralow Molecular Weight Heparins

(2011) Y. Xu et al.   SCIENCE

Production of chondroitin sulfate and chondroitin

(2010) Chiara Schiraldi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Chemoenzymatic Design of Heparan Sulfate Oligosaccharides

(2010) Renpeng Liu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Improved fructosylated chondroitin production by kfoC overexpression in E. coli K4

(2010) Donatella Cimini et al.   JOURNAL OF BIOTECHNOLOGY

Isolation of an Escherichia coli K4 kfoC mutant over-producing capsular chondroitin

(2010) Anna Zanfardino et al.   Microbial Cell Factories

Production of capsular polysaccharide from Escherichia coli K4 for biotechnological applications

(2009) Donatella Cimini et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Two Dermatan Sulfate Epimerases Form Iduronic Acid Domains in Dermatan Sulfate

(2009) Benny Pacheco et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Organ-specific Heparan Sulfate Structural Phenotypes

(2009) Xiaofeng Shi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

AVE5026, a new hemisynthetic ultra-low-molecular-weight heparin for the prevention of venous thromboembolism in patients after total knee replacement surgery - TREK: a dose-ranging study

(2009) M. R. LASSEN et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Description of the chemical and pharmacological characteristics of a new hemisynthetic ultra-low-molecular-weight heparin, AVE5026

(2009) C. VISKOV et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Lessons learned from the contamination of heparin

(2009) Haiying Liu et al.   NATURAL PRODUCT REPORTS

Chondroitin sulphate: A complex molecule with potential impacts on a wide range of biological systems

(2008) Robert M. Lauder   COMPLEMENTARY THERAPIES IN MEDICINE

Immobilization of Heparin: Approaches and Applications

(2008) Robert Linhardt et al.   CURRENT TOPICS IN MEDICINAL CHEMISTRY

Identification of the Active Site of DS-epimerase 1 and Requirement ofN-Glycosylation for Enzyme Function

(2008) Benny Pacheco et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Solution Structures of Chemoenzymatically Synthesized Heparin and Its Precursors

(2008) Zhenqing Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events

(2008) Marco Guerrini et al.   NATURE BIOTECHNOLOGY

Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus Emerged in China

(2008) Y.-J. Zhou et al.   Transboundary and Emerging Diseases