Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Medical Laboratory Technology
Sofie K. M. van Zundert, Pieter H. Griffioen, Lenie van Rossem, Sten P. Willemsen, Yolanda B. de Rijke, Ron H. N. van Schaik, Regine P. M. Steegers-Theunissen, Mina Mirzaian
Summary: This study developed and validated a LC-MS/MS method to quantify five tryptophan metabolites and investigated their correlation with body mass index (BMI) in pregnant women. The results showed a negative correlation between BMI and the analytes TRP, 5-HTP, and 5-HIAA, and a positive correlation with KYN.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemical Research Methods
Cheryl Turner, Helga Refsum, Nasser E. Bastani
Summary: In this study, an analytical method based on liquid chromatography with electrospray ionization coupled to tandem mass spectrometry detection was developed for accurate quantification of various amino acids in human plasma. The method showed excellent linearity, accuracy, precision, sensitivity, and selectivity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nicolas Sambiagio, Jean-Jacques Sauvain, Aurelie Berthet, Reto Auer, Anna Schoeni, Nancy B. Hopf
Summary: Human biomonitoring of oxidative stress often relies on urinary biomarkers such as 8-oxodG and 8-isoprostane, but inconsistent levels reported in scientific literature can be attributed to the various analytical methods used. A newly developed UPLC-MS/MS method allows for simultaneous quantification of both biomarkers with high accuracy and precision. This method shows promise for biomonitoring and epidemiological studies focusing on oxidative damage.
Article
Biochemical Research Methods
Salome Poyer, Ilham Seffouh, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Jose L. Neira, Jean-Yves Salpin, Regis Daniel
Summary: The study developed a new analytical method for efficient differentiation and sensitive detection of different structures of chondroitin sulfate glycosaminoglycans, which was applied to analyze the disaccharide composition of biological reaction media.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Isabel Meister, Pei Zhang, Anirban Sinha, C. Magnus Skold, Asa M. Wheelock, Takashi Izumi, Romanas Chaleckis, Craig E. Wheelock
Summary: This study developed a method for measuring specific gravity in urine using a refractive index detector (RID) in a 96-well-plate format, providing a new solution for metabolomic research on this noninvasive biofluid. By developing an automated LC-MS workflow and utilizing multiple technical internal standards to monitor data quality, over 540 urinary metabolites were successfully identified.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Shengnan Ji, Xiaomeng Liu, Jing Ha, Lianfeng Ai, Zheng Li
Summary: A sensitive and simple LC-MS/MS method was developed for the determination of orelabrutinib in human plasma and cerebrospinal fluid. The method involved simple protein precipitation, efficient separations, and optimized mass spectrometric conditions to increase selectivity and sensitivity. The method was validated for accuracy, precision, selectivity, linearity, recovery, matrix effect, stability, and limits of quantification. It exhibited a lower limit of quantification of 0.50 ng/mL, with intraday and interday precision RSD both less than 15%, and a recovery rate of 85.7%-92.9%. The method was successfully applied for quantitation in clinical patients treated with orelabrutinib.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Masaki Takiwaki, Fumio Nomura, Mamoru Satoh, Sachio Tsuchida, Kazuo Otake, Junko Takagi
Summary: This study aimed to develop an LC/MS/MS-based method for detecting human plasma AVP and compare it with the traditional RIA method. Although there was a weak overall correlation between the results obtained using the two different methods, disagreements were observed at low concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Analytical
Nikita P. Bacalzo, Garret Couture, Ye Chen, Juan J. Castillo, Katherine M. Phillips, Naomi K. Fukagawa, Carlito B. Lebrilla
Summary: A novel method for high-throughput and quantitative analysis of polysaccharides in food samples was developed, successfully quantifying nine polysaccharides with sufficient accuracy and reproducibility. Varied concentrations of polysaccharides were observed in different food samples, highlighting the potential implications for understanding the interactions of dietary polysaccharides, gut microbial community, and human health.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yongjuan Jia, Xingli Liu, Li Xu, Jinbao Zhao, Junjun Ni, Yuanyuan Zhang
Summary: A high-throughput solid-phase extraction method was developed for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry in this study. The method showed good linearities and sensitivity for each steroid hormone within the quantitative range. It has been successfully applied to series of samples from clinical trials with no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Mark E. Jennings, Jay R. Silveira, Kimberly M. Treier, Paula B. Tracy, Dwight E. Matthews
Summary: A novel LC-MS method utilizing both reverse phase C18 column and PGC column was developed to retain both hydrophobic and hydrophilic peptides, addressing the issue of undetected hydrophilic peptides in a C18 nanocolumn setup.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Veronika Pesakova, Hana Brozmanova, Pavel Sistik, Zuzana Kusnirikova, Ivana Kacirova, Milan Grundmann
Summary: A rapid and simple method was developed to determine the total and free concentration of Teriflunomide in the serum of patients with multiple sclerosis. The method showed high sensitivity and can be applied to clinical samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Environmental Sciences
Wenli Zhou, Min Yue, Qin Liu, Feng Wang, Liying Liu, Lu Wang, Xiaoqiang Liu, Meilin Zheng, Hong Xiao, Qunhua Bai, Jieying Gao
Summary: A study developed a method to quantify neonicotinoids in urine and found widespread exposure among adolescents in Chongqing, Southwest China. However, based on reference values, the health risks from exposure to neonicotinoids were low.
Article
Biochemical Research Methods
Gabor Toth, Domonkos Pal, Karoly Vekey, Laszlo Drahos, Lilla Turiak
Summary: Chondroitin sulfate (CS) is a widely studied class of glycosaminoglycans, commonly analyzed based on disaccharide analysis; vacuum evaporation is preferred over lyophilization for solvent exchange, with higher recovery in aqueous solutions; proper storage and handling conditions are essential to prevent decomposition of the resulting disaccharide mixture.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Urology & Nephrology
Colin Bauer, Federica Piani, Mindy Banks, Flor A. Ordonez, Carmen de Lucas-Collantes, Kaori Oshima, Eric P. Schmidt, Igor Zakharevich, Alfons Segarra, Cristina Martinez, Carlos Roncal-Jimenez, Simon C. Satchell, Petter Bjornstad, Marshall Scott Lucia, Judith Blaine, Joshua M. Thurman, Richard J. Johnson, Gabriel Cara-Fuentes
Summary: Our study suggests that alterations in the systemic and glomerular endothelium are present in almost all patients with MCD and SSNS, and circulating factors in the sera of patients during relapse can directly activate glomerular endothelial cells.
KIDNEY INTERNATIONAL REPORTS
(2022)
Article
Critical Care Medicine
Christopher D. Barrett, Hunter B. Moore, Ernest E. Moore, Janice Wang, Negin Hajizadeh, Walter L. Biffl, Lawrence Lottenberg, Purvesh R. Patel, Michael S. Truitt, Robert C. McIntyre, Todd M. Bull, Lee Anne Ammons, Arsen Ghasabyan, James Chandler, Ivor S. Douglas, Eric P. Schmidt, Peter K. Moore, Franklin L. Wright, Ramona Ramdeo, Robert Borrego, Mario Rueda, Achal Dhupa, D. Scott McCaul, Tala Dandan, Pralay K. Sarkar, Benazir Khan, Coimbatore Sreevidya, Conner McDaniel, Heather M. Grossman Verner, Christopher Pearcy, Lorenzo Anez-Bustillos, Elias N. Baedorf-Kassis, Rashi Jhunjhunwala, Shahzad Shaefi, Krystal Capers, Valerie Banner-Goodspeed, Daniel S. Talmor, Angela Sauaia, Michael B. Yaffe
Summary: This study investigated the effects of tissue plasminogen activator (tPA) on pulmonary function in severe COVID-19 respiratory failure. The results showed significant improvement in lung oxygenation in the tPA bolus group compared to the control group. However, there were no statistically significant differences in the improvement of PaO2/FIO2 ratio, composite outcome, ventilator-free days, and in-hospital mortality between the tPA bolus group and the control group.
Article
Medicine, General & Internal
Angela J. Rogers, Deborah Wentworth, Andrew Phillips, Katy Shaw-Saliba, Robin L. Dewar, Neil R. Aggarwal, Abdel G. Babiker, Weizhong Chang, Nila J. Dharan, Victoria J. Davey, Elizabeth S. Higgs, Norman Gerry, Adit A. Ginde, J. W. Awori Hayanga, Helene Highbarger, Jeroen L. Highbarger, Mamta K. Jain, Virginia Kan, Kami Kim, Perrine Lallemand, Bradley G. Leshnower, Joseph K. Lutaakome, Gail Matthews, Ahmad Mourad, Eleftherios Mylonakis, Ven Natarajan, Maria L. Padilla, Lavannya M. Pandit, Roger Paredes, Sarah Pett, Srikanth Ramachandruni, M. Tauseef Rehman, Brad T. Sherman, D. Clark Files, Samuel M. Brown, Michael A. Matthay, B. Taylor Thompson, James D. Neaton, H. Clifford Lane, Jens D. Lundgren
Summary: This study conducted a cross-sectional study of plasma antigen levels in 2540 hospitalized patients with COVID-19, finding that plasma antigen levels were closely associated with the severity of pulmonary disease and clinically important outcomes, with multiple clinical and viral factors associated with baseline plasma antigen levels.
ANNALS OF INTERNAL MEDICINE
(2022)
Article
Chemistry, Applied
Bin Zhang, Deling Shi, Mengmeng Li, Feng Shi, Lianli Chi
Summary: Heparin is a widely used anticoagulant extracted from animal tissues, with porcine intestine mucosa being the main legal source. Differentiating heparins derived from porcine, ovine, and bovine is challenging, especially when ruminant heparin is adulterated into porcine heparin. This study establishes a quantitative mass spectrometry method to selectively monitor marker saccharides, allowing for the differentiation of porcine heparin adulterated with low amounts of ruminant heparin, ensuring the authenticity and safety of heparin drugs.
CARBOHYDRATE POLYMERS
(2023)
Article
Medicine, General & Internal
Nathan I. Shapiro, Ivor S. Douglas, Roy G. Brower, Samuel M. Brown, Matthew C. Exline, Adit A. Ginde, Michelle N. Gong, Colin K. Grissom, Douglas Hayden, Catherine L. Hough, Weixing Huang, Theodore J. Iwashyna, Alan E. Jones, Akram Khan, Poying Lai, Kathleen D. Liu, Chadwick D. Miller, Katherine Oldmixon, Pauline K. Park, Todd W. Rice, Nancy Ringwood, Matthew W. Semler, Jay S. Steingrub, Daniel Talmor, B. Taylor Thompson, Donald M. Yealy, Wesley H. Self
Summary: This study compared the effects of a restrictive fluid strategy and a liberal fluid strategy in the treatment of septic patients. The results showed that there was no significant difference in overall mortality before discharge home by day 90 between the two strategies.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Review
Cell Biology
Alicia N. Rizzo, Eric P. Schmidt
Summary: The alveolar epithelial glycocalyx is a layer of glycosaminoglycans (GAGs) and proteoglycans on the surface of alveolar epithelial cells. Recent studies have shown that the degradation of the glycocalyx is associated with acute respiratory distress syndrome (ARDS) in mice models and respiratory failure patients. This suggests that the alveolar epithelial glycocalyx plays a crucial role in the pathogenesis of ARDS.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Physiology
Christina Sul, Caitlin Lewis, Nathan Dee, Nana Burns, Kaori Oshima, Eric Schmidt, Christine Vohwinkel, Eva Nozik
Summary: Acute respiratory distress syndrome (ARDS) is a significant cause of morbidity and mortality in critically ill patients. This study investigates the protective effects of the R213G variant of extracellular superoxide dismutase (EC-SOD) in a methicillin-resistant S. aureus (MRSA) pneumonia model. The results suggest that the R213G EC-SOD variant preserves the integrity of the alveolar-capillary interface, attenuates proinflammatory cytokine levels, reduces neutrophil recruitment and neutrophil extracellular trap (NET) generation, and prevents extrapulmonary dissemination of bacteria.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Critical Care Medicine
Karan Rai, Ivor S. Douglas, Anuj B. Mehta
Summary: The weekend effect, which refers to increased mortality in patients admitted to hospitals on weekends, has been studied in intensive care units with conflicting results. This study found no significant difference in hospital mortality for patients admitted and initiated on mechanical ventilation on weekends compared to weekdays.
JOURNAL OF INTENSIVE CARE MEDICINE
(2023)
Article
Respiratory System
Anuj B. Mehta, Jennifer K. Taylor, Gwenyth Day, Trevor C. Lane, Ivor S. Douglas
Summary: A retrospective cohort study using the Nationwide Readmissions Database was conducted to analyze treatment selection for patients with cardiopulmonary failure. The results showed that female patients, those with Medicaid, and those living in low-income neighborhoods were less likely to receive extracorporeal membrane oxygenation (ECMO) treatment.
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2023)
Article
Critical Care Medicine
August Longino, Katharine Martin, Katarina Leyba, Gabriel Siegel, Edward Gill, Ivor S. Douglas, Joseph Burke
Summary: Venous congestion is often overlooked as a cause of mortality in critically ill patients. A novel noninvasive Venous Excess Ultrasound (VExUS) score, which measures venous congestion using inferior vena cava (IVC) diameter and Doppler flow, has shown a strong correlation with right atrial pressure (RAP). This suggests that VExUS may be a useful tool for assessing and managing venous congestion in a variety of critical illnesses.
Letter
Medicine, General & Internal
Samuel A. Hofacker, Eric P. Schmidt
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Health Care Sciences & Services
Jason N. Mansoori, Brendan J. Clark, Edward P. Havranek, Ivor S. Douglas
Summary: Choice architecture may affect fluid resuscitation decisions in sepsis regardless of patient conditions, warranting further investigation in real-world contexts. These effects should be considered when implementing practice guidelines.
MDM POLICY & PRACTICE
(2022)
Article
Medicine, General & Internal
Ivan O. Rosas, Norbert Brau, Michael Waters, Ronaldo C. Go, Atul Malhotra, Bradley D. Hunter, Sanjay Bhagani, Daniel Skiest, Sinisa Savic, Ivor S. Douglas, Julia Garcia-Diaz, Mariam S. Aziz, Nichola Cooper, Taryn Youngstein, Lorenzo Del Sorbo, David J. De La Zerda, Andrew Ustianowski, Antonio Cubillo Gracian, Kevin G. Blyth, Jordi Carratala, Bruno Francois, Thomas Benfield, Derrick Haslem, Paolo Bonfanti, Cor H. van der Leest, Nidhi Rohatgi, Lothar Wiese, Charles Edouard Luyt, Rebecca N. Bauer, Fang Cai, Ivan T. Lee, Balpreet Matharu, Louis Metcalf, Steffen Wildum, Emily Graham, Larry Tsai, Min Bao
Summary: Tocilizumab did not improve mortality in COVID-19 patients through day 60, but it shortened hospital stay and did not impair viral clearance or antibody response. No new safety signals were observed. Future research may explore biomarkers for patient selection and combination therapy.
Review
Critical Care Medicine
Michael R. Pinsky, Maurizio Cecconi, Michelle S. Chew, Daniel De Backer, Ivor Douglas, Mark Edwards, Olfa Hamzaoui, Glenn Hernandez, Greg Martin, Xavier Monnet, Bernd Saugel, Thomas W. L. Scheeren, Jean-Louis Teboul, Jean-Louis Vincent
Summary: Hemodynamic monitoring plays a crucial role in acute care settings, but its effectiveness is hard to measure. This review summarizes the effectiveness of monitoring-linked resuscitation strategies, including process-specific monitoring, personalized resuscitation approaches, and machine learning methods. Future clinical trials should focus on process-specific monitoring to improve patient outcomes.
