Polycistronic expression of the mitochondrial steroidogenic P450scc system in the HEK293T cell line

Impairment of a membrane-targeting protein translated from a downstream gene of a “self-cleaving” T2A peptide conjunction

(2018) Sudarat Hadpech et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression of Cholesterol Hydroxylase/Lyase System Proteins in Yeast S. cerevisiae Cells as a Self-Processing Polyprotein

(2017) Vera S. Efimova et al.   MOLECULAR BIOTECHNOLOGY

Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system

(2017) He Sun et al.   PLoS One

Effects of acrolein on the production of corticosterone in male rats

(2016) Yung-Hsing Yeh et al.   STEROIDS

Membrane-Mediated Protein-Protein Interactions of Cholesterol Side-Chain Cleavage Cytochrome P450 with its Associated Electron Transport Proteins

(2016) Clemens Kubeil et al.   ChemPlusChem

Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells

(2015) Jake Chng et al.   mAbs

2A self-cleaving peptide-based multi-gene expression system in the silkworm Bombyx mori

(2015) Yuancheng Wang et al.   Scientific Reports

Coexpression of cellulases in Pichia pastoris as a self-processing protein fusion

(2015) Juliana de Amorim Araújo et al.   AMB Express

Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

(2014) Beth A. Rasala et al.   PLoS One

Expression of Multiple Transgenes from a Single Construct Using Viral 2A Peptides in Drosophila

(2014) Richard W. Daniels et al.   PLoS One

Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations

(2014) Yao-Cheng Lin et al.   Nature Communications

A novel multigene expression construct for modification of glycerol metabolism in Yarrowia lipolytica

(2013) Ewelina Celińska et al.   Microbial Cell Factories

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Human cytochromes P450 in health and disease

(2013) D. W. Nebert et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Protein Coexpression Using FMDV 2A: Effect of “Linker” Residues

(2013) Ekaterina Minskaia et al.   Biomed Research International

Antisteroidogenic effects of hydrogen peroxide on rat granulosa cells

(2012) Po-Ling Yu et al.   FREE RADICAL RESEARCH

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice

(2011) Jin Hee Kim et al.   PLoS One

From structure and functions of steroidogenic enzymes to new technologies of gene engineering

(2010) L. A. Novikova et al.   BIOCHEMISTRY-MOSCOW

Localization and functional activity of cytochrome P450 side chain cleavage enzyme (CYP11A1) in the adult rat kidney

(2010) Melina A. Pagotto et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm

(2010) Sun-Hwa Ha et al.   PLANT BIOTECHNOLOGY JOURNAL

Signal sequence is still required in genes downstream of “autocleaving” 2A peptide for secretary or membrane-anchored expression

(2009) Jun Yan et al.   ANALYTICAL BIOCHEMISTRY

Inhibition of 2A-mediated ‘cleavage’ of certain artificial polyproteins bearing N-terminal signal sequences

(2009) Pablo de Felipe et al.   Biotechnology Journal