Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells

Nonspecific interactions of chromatin with immunoglobulin G and protein A, and their impact on purification performance

(2014) Pete Gagnon et al.   JOURNAL OF CHROMATOGRAPHY A

Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells

(2013) Leon P. Pybus et al.   BIOTECHNOLOGY AND BIOENGINEERING

Predicting the expression of recombinant monoclonal antibodies in Chinese hamster ovary cells based on sequence features of the CDR3 domain

(2013) Leon P. Pybus et al.   BIOTECHNOLOGY PROGRESS

Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications

(2013) Ekaterina Minskaia et al.   BMC BIOTECHNOLOGY

Control of IgG LC:HC ratio in stably transfected CHO cells and study of the impact on expression, aggregation, glycosylation and conformational stability

(2013) Steven C.L. Ho et al.   JOURNAL OF BIOTECHNOLOGY

Characterization and removal of aggregates formed by nonspecific interaction of IgM monoclonal antibodies with chromatin catabolites during cell culture production

(2013) Hui Theng Gan et al.   JOURNAL OF CHROMATOGRAPHY A

Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for Monoclonal Antibody Expression Level and Quality in CHO Cells

(2013) Steven C. L. Ho et al.   PLoS One

An Internal Ribosome Entry Site (IRES) Mutant Library for Tuning Expression Level of Multiple Genes in Mammalian Cells

(2013) Esther Y. C. Koh et al.   PLoS One

Protein Coexpression Using FMDV 2A: Effect of “Linker” Residues

(2013) Ekaterina Minskaia et al.   Biomed Research International

Flp and Cre expressed from Flp–2A–Cre and Flp–IRES–Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange

(2012) Rachelle P. Anderson et al.   NUCLEIC ACIDS RESEARCH

Impact of gene vector design on the control of recombinant monoclonal antibody production by chinese hamster ovary cells

(2011) Sarah L. Davies et al.   BIOTECHNOLOGY PROGRESS

IRES-mediated Tricistronic vectors for enhancing generation of high monoclonal antibody expressing CHO cell lines

(2011) Steven C.L. Ho et al.   JOURNAL OF BIOTECHNOLOGY

2A Peptide-based, Lentivirus-mediated Anti-death Receptor 5 Chimeric Antibody Expression Prevents Tumor Growth in Nude Mice

(2011) Meng Li et al.   MOLECULAR THERAPY

2A peptides provide distinct solutions to driving stop-carry on translational recoding

(2011) Pamila Sharma et al.   NUCLEIC ACIDS RESEARCH

High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice

(2011) Jin Hee Kim et al.   PLoS One

Combination of the 2A/furin technology with an animal component free cell line development platform process

(2010) Thomas Jostock et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Coexpression of double or triple copies of the rabies virus glycoprotein gene using a ‘self-cleaving’ 2A peptide-based replication-defective human adenovirus serotype 5 vector

(2010) Yeping Tan et al.   BIOLOGICALS

Cell line-specific control of recombinant monoclonal antibody production by CHO cells

(2010) Peter M. O'Callaghan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Development trends for human monoclonal antibody therapeutics

(2010) Aaron L. Nelson et al.   NATURE REVIEWS DRUG DISCOVERY

A study of human furin specificity using synthetic peptides derived from natural substrates, and effects of potassium ions

(2009) Mario A. Izidoro et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Inhibition of 2A-mediated ‘cleavage’ of certain artificial polyproteins bearing N-terminal signal sequences

(2009) Pablo de Felipe et al.   Biotechnology Journal

Vector fragmentation: Characterizing vector integrity in transfected clones by Southern blotting

(2009) Say Kong Ng et al.   BIOTECHNOLOGY PROGRESS

Dissection of a co-translational nascent chain separation event

(2008) Victoria A. Doronina et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Mutated polyadenylation signals for controlling expression levels of multiple genes in mammalian cells

(2008) Yuansheng Yang et al.   BIOTECHNOLOGY AND BIOENGINEERING

A study of monoclonal antibody-producing CHO cell lines: What makes a stable high producer?

(2008) Janet Chusainow et al.   BIOTECHNOLOGY AND BIOENGINEERING

A clone screening method using mRNA levels to determine specific productivity and product quality for monoclonal antibodies

(2008) Christina J. Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Local secretion of anti-CTLA-4 enhances the therapeutic efficacy of a cancer immunotherapy with reduced evidence of systemic autoimmunity

(2008) Andrew D. Simmons et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition

(2008) S Yang et al.   GENE THERAPY

Site-Specific Release of Nascent Chains from Ribosomes at a Sense Codon

(2008) V. A. Doronina et al.   MOLECULAR AND CELLULAR BIOLOGY

On the Optimal Ratio of Heavy to Light Chain Genes for Efficient Recombinant Antibody Production by CHO Cells

(2005) Stefan Schlatter et al.   BIOTECHNOLOGY PROGRESS