Article
Biochemistry & Molecular Biology
Gregory E. Conner
Summary: The redox status of cells is crucial for cellular activities; H2O2 metabolism regulates the redox status by modifying protein cysteine oxidation; Calcium-stimulated epithelial Duox H2O2 synthesis is transient and prevents harmful changes in redox tone following continuous stimulation.
Review
Biochemistry & Molecular Biology
Anthony L. Sylvester, David X. Zhang, Sophia Ran, Natalya S. Zinkevich
Summary: Reactive oxygen species (ROS) play important roles in health and disease, with both beneficial and harmful effects. Different sources of ROS have different roles in various pathologies. NOX1 is associated with hypertension through ROS production, making it a promising therapeutic target. NOX2 and NOX4 produce hydrogen peroxide, influencing cellular processes. Increased ROS production from NOX5 contributes to atherosclerosis.
Article
Pharmacology & Pharmacy
Anantha Harijith, Prathima Basa, Alison Ha, Jaya Thomas, Anjum Jafri, Panfeng Fu, Peter M. MacFarlane, Thomas M. Raffay, Viswanathan Natarajan, Tara Sudhadevi
Summary: This study aimed to determine the possible role of AT2 epithelial cell NOX4 in epithelial cell death from hyperoxic acute lung injury (HALI). The results demonstrated that deletion of the Nox4 gene or silencing NOX4 can attenuate epithelial cell apoptosis and mitochondrial ROS production, thus protecting the lungs from severe lung injury.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Toxicology
Masahide Inoue, Koji Sakamoto, Atsushi Suzuki, Shinya Nakai, Akira Ando, Yukihiko Shiraki, Yoshio Nakahara, Mika Omura, Atsushi Enomoto, Ikuhiko Nakase, Makoto Sawada, Naozumi Hashimoto
Summary: Our study using a murine model demonstrated that the pulmonary toxicity of silica nanoparticles depended on their physico-chemical properties through distinct mechanisms. Cellular uptake of larger particles by macrophages decreased, while surface amine modification modulated endosomal reactive oxygen species (ROS) signaling via NADPH oxidase 2 (NOX2), both of which were involved in mitigating the immune response in macrophages and resulting lung injury.
PARTICLE AND FIBRE TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Giulia Blandino, Mara Fiorani, Barbara Canonico, Rita De Matteis, Andrea Guidarelli, Mariele Montanari, Gloria Buffi, Lucia Coppo, Elias S. J. Arner, Orazio Cantoni
Summary: Long-term treatment of schizophrenia with clozapine may lead to metabolic disorders, and this study found that clozapine can inhibit adipogenesis and reduce reactive oxygen species produced by NADPH oxidase. Clozapine also suppresses antioxidant responses and promotes mitochondrial superoxide formation, which may be associated with insulin resistance and metabolic syndrome. These cellular events may provide insights into the molecular mechanisms underlying the adverse metabolic effects of clozapine.
Article
Cell Biology
Yueguo Wang, Zhenxing Ding, Youhui Tu, Xu Wu, Wenying Zhang, Shuang Ji, Jilong Shen, Li Zhang, Huimei Wu, Guanghe Fei
Summary: The study demonstrates that Poldip2 mediates LPS-induced oxidative stress and inflammation through interaction with Nox4, regulated by the PI3K-AKT signaling pathway. Targeting Poldip2 could be a beneficial therapeutic strategy for acute lung injury.
MEDIATORS OF INFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Yi Wen, Hansen Chen, Lu Zhang, Meiling Wu, Feng Zhang, Dan Yang, Jiangang Shen, Jianping Chen
Summary: 18-beta-glycyrrhetinic acid can promote the production of ROS and RNS through activating NADPH oxidases and iNOS, decreasing GSH and GPX activity, subsequently exacerbating lipid peroxidation and triggering ferroptosis in triple-negative breast cancer cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yanfeng Sun, Weihong Liang, Hui Cheng, Huan Wang, Dong Lv, Wei Wang, Modan Liang, Chen Miao
Summary: The study reveals that under salt stress, plasma membrane NADPH Oxidase-derived ROS promote mitochondrial alkalization, and mitochondrial alkalization may be regulated through certain pathways.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Cell Biology
Daniel Simoes de Jesus, Paula Bargi-Souza, Vinicius Cruzat, Vijay Yechoor, Angelo Rafael Carpinelli, Rodrigo Antonio Peliciari-Garcia
Summary: The pancreatic beta cells circadian clock and NADPH oxidase (NOX) family play important roles in glucose metabolism. Absence of BMAL1 may trigger an increase in NOX2-derived reactive oxygen species (ROS) in pancreatic beta cells, leading to inhibition of insulin secretion.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mara Fiorani, Rita De Matteis, Barbara Canonico, Giulia Blandino, Alessandro Mazzoli, Mariele Montanari, Andrea Guidarelli, Orazio Cantoni
Summary: The conversion of human SW872 preadipocytes to mature adipocytes involves time-dependent changes in differentiation markers' expression, morphological changes, and accumulation of lipid droplets. Reactive oxygen species (ROS) formation was significant at specific time points during differentiation, with NADPH oxidase (NOX)-2 playing a role. Mitochondrial ROS (mROS) were only detected in the late phase of differentiation, indicating potential mitochondrial dysfunction. These findings suggest a potential link between ROS, adipogenesis, and adipose tissue dysfunction in SW872 cells.
Article
Cell Biology
Minwoo Baek, Wijeong Jang, Changsoo Kim
Summary: This study identifies dual oxidase (duox) as a source of neuronal oxidative stress associated with animal lifespan. The research found that reducing oxidative stress in neurons extends the lifespan of Drosophila melanogaster, with duox playing a crucial role in this process.
Article
Biochemistry & Molecular Biology
Stephenson B. Owusu, Elodie Hudik, Celine Ferard, Sophie Dupre-Crochet, Eric C. D. K. Addison, Kwasi Preko, Tania Bizouarn, Chantal Houee-Levin, Laura Baciou
Summary: Simulating oxidative stress with gamma irradiation leads to shortened half-lives of neutrophils but pre-activates surviving cells to produce superoxide anions. Incomplete assembly of the NADPH oxidase complex after irradiation significantly enhances neutrophil reactivity.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Rana M. Nassif, Elias Chalhoub, Pia Chedid, Margarita Hurtado-Nedelec, Elia Raya, Pham My-Chan Dang, Jean-Claude Marie, Jamel El-Benna
Summary: Metformin exhibits anti-inflammatory effects by inhibiting the differentiation of human monocytes into proinflammatory macrophages and by limiting ROS production by macrophages via the activation of AMPK.
Article
Microbiology
Hasnaa Maksouri, Dounia Darif, Jerome Estaquier, Myriam Riyad, Christophe Desterke, Meryem Lemrani, Pham My-Chan Dang, Khadija Akarid
Summary: This study evaluated the impact of Moroccan strains of L. major and L. tropica on PMNs. The results showed that L. tropica inhibited O-2(-) production, while L. major did not. Additionally, Leishmania soluble antigens from both species inhibited O-2(-) induction.
Article
Agronomy
Jie Li, Lidan Feng, Dong Li, Xianglin Liu, Yangyang Pan, Jing He, Junxia Zhang
Summary: This study investigates the role of ROS mediated by NADPH oxidase in the growth of F. solani. The results demonstrate that NCF2 gene plays a significant role in regulating the activity of NADPH oxidase and the expression of ROS metabolism enzyme genes, which affects colony growth in F. solani. Furthermore, exogenous H2O2 treatment also influences the growth and ROS metabolism of F. solani.
Article
Cell Biology
Jing Jin, Adrian Britschgi, Anna M. Schlafli, Magali Humbert, Deborah Shan-Krauer, Jasmin Batliner, Elena A. Federzoni, Marion Ernst, Bruce E. Torbett, Shida Yousefi, Hans-Uwe Simon, Mario P. Tschan
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2018)
Article
Microbiology
Masaharu Iwasaki, Petra Minder, Yingyun Cai, Jens H. Kuhn, John R. Yates, Bruce E. Torbett, Juan C. de la Torre
Article
Multidisciplinary Sciences
Guoli Shi, Stosh Ozog, Bruce E. Torbett, Alex A. Compton
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Medicine, Research & Experimental
Stosh Ozog, Craig X. Chen, Elizabeth Simpson, Olivia Garijo, Nina D. Timberlake, Petra Minder, Els Verhoeyen, Bruce E. Torbett
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2019)
Article
Hematology
Stosh Ozog, Nina D. Timberlake, Kip Hermann, Olivia Garijo, Kevin G. Haworth, Guoli Shi, Christopher M. Glinkerman, Lauren E. Schefter, Saritha D'Souza, Elizabeth Simpson, Gabriella Sghia-Hughes, Raymond R. Carillo, Dale L. Boger, Hans-Peter Kiem, Igor Slukvin, Byoung Y. Ryu, Brian P. Sorrentino, Jennifer E. Adair, Scott A. Snyder, Alex A. Compton, Bruce E. Torbett
Article
Medicine, Research & Experimental
Krista A. Delviks-Frankenberry, Daniel Ackerman, Nina D. Timberlake, Maria Hamscher, Olga A. Nikolaitchik, Wei-Shau Hu, Bruce E. Torbett, Vinay K. Pathak
MOLECULAR THERAPY-NUCLEIC ACIDS
(2019)
Article
Biochemistry & Molecular Biology
Li-Tao Guo, Rebecca L. Adams, Han Wan, Nicholas C. Huston, Olga Potapova, Sara Olson, Christian M. Gallardo, Brenton R. Graveley, Bruce E. Torbett, Anna Marie Pyle
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Review
Virology
Shentian Zhuang, Bruce E. Torbett
Summary: This paper reviews the interplay between the HIV-1 capsid and host factors during early stages of viral replication, highlighting the structure of the capsid, host factor interactions, and screening techniques. Understanding these interactions can inform HIV-1 pathogenesis and the development of capsid-centric antiretroviral therapeutics. The discovery of the capsid-targeting inhibitor GS-6207 represents a significant advancement in this field.
Article
Biochemistry & Molecular Biology
Yisong Deng, John A. Hammond, Raymond Pauszek, Stosh Ozog, Ilean Chai, Jessica Rabuck-Gibbons, Rajan Lamichhane, Scott C. Henderson, David P. Millar, Bruce E. Torbett, James R. Williamson
Summary: Most GCC species are mainly composed of monomeric or dimeric Gag molecules bound to ribosomal complexes, representing a population of Gag not yet functionally committed for incorporation into viable virion precursors. A subset of large GCC complexes, with more than 20 Gag molecules, may be equivalent to membrane-associated puncta as genuine assembling-virus intermediates. These findings provide a rationale for the existence of diverse GCCs and lay the foundation for characterizing on-pathway intermediates in early virus assembly.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Christian M. Gallardo, Shiyi Wang, Daniel J. Montiel-Garcia, Susan J. Little, Davey M. Smith, Andrew L. Routh, Bruce E. Torbett
Summary: This study overcomes technical challenges in RNA virus sequencing by developing a nanopore-based method to accurately sequence viral genomes from clinical samples. The method can detect rare viral genomes at <1% frequency, facilitating the study of genetic resistance to antiviral therapy and immune pressure.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shiyi Wang, Stephanea L. Sotcheff, Christian M. Gallardo, Elizabeth Jaworski, Bruce E. Torbett, Andrew L. Routh
Summary: Viruses adapt to their environments through acquiring new single-nucleotide variants (SNVs) and recombination events. However, studying the correlation between recombination events and SNVs has been challenging due to genetic complexity and lack of tools. This study improved a pipeline to measure linkage disequilibrium between recombination events and SNVs, and revealed their role in viral evolution.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Kristina Seiler, Magali Humbert, Petra Minder, Iris Mashimo, Anna M. Schlafli, Deborah Krauer, Elena A. Federzoni, Bich Vu, James J. Moresco, John R. Yates, Martin C. Sadowski, Ramin Radpour, Thomas Kaufmann, Jean-Emmanuel Sarry, Joern Dengjel, Mario P. Tschan, Bruce E. Torbett
Summary: This study found that HK3 is dispensable for glycolytic activity in AML cells while promoting cell survival, possibly through direct interaction with the BH3-only protein BIM during ATRA-induced neutrophil differentiation.
CELL DEATH & DISEASE
(2022)
Article
Hematology
Saritha S. D'Souza, Akhilesh Kumar, John Maufort, Jason T. Weinfurter, Matthew Raymond, Nick S. Strelchenko, Elizabeth Perrin, Jennifer Coonen, Andres Mejia, Heather A. Simmons, Bruce E. Torbett, Matthew Reynolds, James A. Thomson, Igor I. Slukvin
Summary: The administration of ex vivo expanded somatic myeloid progenitors is explored as a way to accelerate myeloid recovery. Recent studies have shown the potential of hematopoietic progenitors derived from MHC homozygous iPSCs in treating cytopenia with good tolerance and safety in experimental animals.
Article
Biochemistry & Molecular Biology
Christian M. Gallardo, Anh-Viet T. Nguyen, Andrew L. Routh, Bruce E. Torbett
Summary: Alternative splicing is important for viral proliferation and gene expression regulation. Traditional RNA-seq methods have limitations in coverage, while the CASPR method allows simultaneous analysis of viral and host cell transcripts, improving coverage and detection of long transcripts.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biology
Stephanea Sotcheff, Yiyang Zhou, Jason Yeung, Yan Sun, John E. Johnson, Bruce E. Torbett, Andrew L. Routh
Summary: Genetic recombination is important for viruses to adapt to new environments. However, detecting recombination events in high-throughput sequencing data is challenging due to the diverse nature of these events. In this study, the algorithm ViReMa was updated to accurately detect recombination events in longer reads and provide standardized output reports for multiple types of recombinant species.