Review
Cell Biology
Gillian DeWane, Alicia M. Salvi, Kris A. DeMali
Summary: The focus has long been on the actin cytoskeleton's role in controlling cell shape, migration, and contact establishment, but recent studies suggest that metabolic adjustments in cells provide the energy necessary for cytoskeletal remodeling.
JOURNAL OF CELL SCIENCE
(2021)
Review
Multidisciplinary Sciences
Apratim Bajpai, Rui Li, Weiqiang Chen
Summary: Aging is a chronic and complex process that results in degenerative physical and biological changes in living organisms, affecting the mechanobiological features of cells. These mechanobiological changes can lead to dysfunctions and diseases in various organ systems. Understanding the mechanobiological effects of aging is important in developing strategies to halt and reverse the aging process.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Biology
Nicholas M. Cronin, Kris A. DeMali
Summary: This article summarizes how cells in the human body remodel their cytoskeleton in response to force, focusing on the actin cytoskeleton and its anchorage at adhesion sites. It examines the complex and coordinated response of cells to force, including changes in cell shape and the formation of new actin filaments. The article also discusses the current understanding of how the actin cytoskeleton is tethered to adhesion sites and highlights recent studies on its remodeling in response to force.
Article
Engineering, Biomedical
Mark Colasurdo, Elisa B. Nieves, Marc A. Fernandez-Yague, Christian Franck, Andres J. Garcia
Summary: Cell-extracellular matrix forces play a crucial role in regulating various physiological and pathological processes. However, the study of mechanobiology in three-dimensional systems is still limited. In this study, a platform integrating a synthetic hydrogel system with 3D traction force microscopy was developed to evaluate deformation and force responses within synthetic microenvironments. The findings shed light on the contributions of adhesive peptide density and polymer density, as well as matrix stiffness, to 3D force generation by fibroblasts. Rho-kinase-dependent contractility and vinculin expression were also found to be essential for significant 3D force generation. This research provides valuable insights into mechanobiology and how cells sense and transmit forces in three dimensions.
Article
Biochemistry & Molecular Biology
Siyuan Huang, Zhu Chen, Xiaoqiang Hou, Kuankuan Han, Bingshe Xu, Miao Zhang, Shukai Ding, Yongtao Wang, Yingjun Yang
Summary: Mechanical stimulation is believed to be a potential cause of acral melanoma. This study analyzed the influence of cyclic straining on melanoma cell proliferation and explored the mechanotransduction mechanism of promoted proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Carolyn E. Keating, D. Kacy Cullen
Summary: Traumatic brain injury is caused by extreme mechanical forces to the brain, leading to cellular responses and potential long-term dysfunction and degeneration. Understanding the biomechanics of TBI and how cells respond to these forces is crucial for identifying therapeutic targets for trauma-related sequelae.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Ophthalmology
Haiyan Li, Ayushi Singh, Kristin M. Perkumas, W. Daniel Stamer, Preethi S. Ganapathy, Samuel Herberg
Summary: This study investigates the molecular mechanisms of TGF-beta2 modulation of YAP/TAZ activity in human SC cells and examines the potential of pharmacological YAP/TAZ inhibition to improve outflow tissue dysfunction under glaucomatous conditions.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Engineering, Biomedical
Qian Sun, Yong Hou, Zhiqin Chu, Qiang Wei
Summary: Cell behaviors and functions show distinct contrast in different mechanical microenvironment. Flexible materials have great potential to sense and adapt to mechanical changes, promoting cellular mechanosensing and downstream phenotypes.
BIOACTIVE MATERIALS
(2022)
Article
Cardiac & Cardiovascular Systems
Davi M. Lyra-Leite, Andrew P. Petersen, Nethika R. Ariyasinghe, Nathan Cho, Megan L. McCain
Summary: This study demonstrates that the architecture of the mitochondrial network in cardiac myocytes is strongly influenced by cell shape and moderately influenced by ECM rigidity. The results indicate that mitochondrial volume increases with cell aspect ratio on all substrates, and mitochondrial surface area to volume ratio decreases as aspect ratio increases. Additionally, large mitochondria are more prevalent in cardiac myocytes with higher aspect ratio, and for specific aspect ratios, mitochondria are smaller as ECM rigidity increases.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2021)
Article
Biophysics
Josephine Solowiej-Wedderburn, Carina M. Dunlop
Summary: Tissue stiffness plays a crucial role in cell behavior, and the arrangement of adhesions between cells and substrate is also important in determining the effective substrate stiffness experienced by cells. Different adhesion patterns result in equivalent mean cell deformations but different gel stiffnesses. Energy considerations can explain the observed focal adhesion growth and elongation on stiff substrates, as well as the absence of focal adhesions on soft substrates. The simulations suggest that fewer and larger adhesions, randomly placed, are preferred and result in realistic cell shapes.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Eva Kulshammer, Merve Kilinc, Gabor Csordas, Tina Bresser, Hendrik Nolte, Mirka Uhlirova
Summary: Cancer development is affected by aberrant sensing and interpretation of mechanical cues and force-generating properties. In this study, the researchers identified the actin crosslinking protein Cheerio (Cher) and its mechanosensitive region (MSR) as instrumental to the malignancy of Ras-driven tumors in Drosophila epithelia. They showed that stimulating myosin activity can rescue the impaired growth and cytoskeletal contractility of cher-deficient tumors. The study also found that Cher interacts with several components of the cell cortex, including Bbg, Kst, and 14-3-3 epsilon, which were identified as tumor suppressors. This highlights the importance of Cher in controlling the contractile state of the cytoskeleton through interactions with myosin and specific components of the cell cortex.
Review
Geriatrics & Gerontology
Danyuan Huang, Shuo Chen, Ding Xiong, Han Wang, Li Zhu, Yuanyuan Wei, Yuyu Li, Shujuan Zou
Summary: Cells need to sense and adapt to mechanical changes in their environment. Mitochondrial dynamics and the cytoskeleton play crucial roles in this process. However, the mechanisms underlying the integration of mechanosensing, mechanotransduction, and metabolic reprogramming remain poorly understood.
Review
Biochemistry & Molecular Biology
Elizabeth R. Kahle, Neil Patel, Harini B. Sreenivasappa, Michele S. Marcolongo, Lin Han
Summary: Mechanosensing at the interface of a cell and its surrounding microenvironment plays a crucial role in physiological processes. This review discusses the use of atomic force microscopy (AFM) integrated with immunofluorescence imaging to probe mechanobiology at the cell-matrix interface. It highlights the investigation of pericellular matrix biomechanics, cellular biomechanics, and mechanotransduction in various tissues. The review also presents technical advances that have facilitated more in-depth studies of mechanobiology.
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Nadia Chandra Sekar, Sergio Aguilera Suarez, Ngan Nguyen, Austin Lai, Peter Thurgood, Ying Zhou, Chanly Chheang, Scott Needham, Elena Pirogova, Karlheinz Peter, Khashayar Khoshmanesh, Sara Baratchi
Summary: Endothelial cells, which line blood vessels, are affected by biophysical cues and play an important role in vascular health and disease. This study investigated the impact of vessel stiffness on endothelial cells and found that cells cultured on softer substrates showed different cellular alignment and morphology compared to cells on stiffer substrates.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Cell Biology
Benjamin T. Goult, Magdalena von Essen, Vesa P. Hytonen
Summary: Mechanical signals play a crucial role in the functioning of organisms. Cells can sense physical forces and respond to them, and mechanical linkages connect different regions of the cell, allowing it to function as a mechanical system. The coordination of mechanical linkages enables the whole cell, its neighbors, tissues, and organs to function properly. Defects in these mechanical linkages can lead to the development of diseases.
JOURNAL OF CELL SCIENCE
(2022)
Article
Engineering, Biomedical
Kazuaki Nagayama, Shunsuke Saito, Takeo Matsumoto
BIO-MEDICAL MATERIALS AND ENGINEERING
(2015)
Article
Biochemistry & Molecular Biology
Sohei Nakayama, Kazushi Arima, Kotoe Kawai, Kurato Mohri, Chihiro Inui, Wakana Sugano, Hibiki Koba, Kentaro Tamada, Yudai J. Nakata, Kouji Kishimoto, Miyuki Arai-Shindo, Chiaki Kojima, Takeo Matsumoto, Toshihiko Fujimori, Kiyokazu Agata, Noriko Funayama
Article
Biophysics
Kazuaki Nagayama, Yumi Hamaji, Yuji Sato, Takeo Matsumoto
JOURNAL OF BIOMECHANICS
(2015)
Article
Biophysics
Shukei Sugita, Takeo Matsumoto
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2017)
Article
Biophysics
Atsutaka Tamura, Sadayuki Hayashi, Takeo Matsumoto
JOURNAL OF MECHANICS IN MEDICINE AND BIOLOGY
(2016)
Article
Cell & Tissue Engineering
Kazuaki Nagayama, Yuki Yahiro, Takeo Matsumoto
CELLULAR AND MOLECULAR BIOENGINEERING
(2013)
Article
Developmental Biology
Yusuke Hara, Kazuaki Nagayama, Takamasa S. Yamamoto, Takeo Matsumoto, Makoto Suzuki, Naoto Ueno
DEVELOPMENTAL BIOLOGY
(2013)
Article
Biophysics
Eijiro Maeda, Yoshinori Atsumi, Mai Ishiguro, Kazuaki Nagayama, Takeo Matsumoto
JOURNAL OF BIOMECHANICS
(2019)
Article
Engineering, Biomedical
Atsutaka Tamura, Jun-ichi Hongu, Takeo Matsumoto
CLINICAL BIOMECHANICS
(2019)
Article
Biochemistry & Molecular Biology
Jeonghyun Kim, Takashi Inagaki, Junko Sunaga, Taiji Adachi, Takeo Matsumoto
Summary: This study elucidated the multicellular behaviors of osteocytic spheroids in response to osteogenesis supplements. The results showed that the supplements altered the spheroids' behaviors by affecting their size, fusion speed, and collagen embedding. Actin filaments were identified as the driving force behind these changes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Yong Fan, Junfeng Wang, Jeonghyun Kim, Eijiro Maeda, Takeo Matsumoto
Summary: The deformation of the cell nucleus can enhance transcription, translation, and protein expression. Excessive stretch of smooth muscle cell nuclei caused by hypertension may lead to wall thickening in arteries. This study explains the unresponsiveness of the nuclei to axial stretch through a novel cell model.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Hiroshi Watanabe, Kohei Murase, Dongwook Kim, Takeo Matsumoto, Tokifumi Majima
Summary: The effects of the proximal tibial slope angle on the proximal tibial epiphysis were investigated using finite element analysis. Results showed a significant correlation between proximal tibial slope angles over 12 degrees and the risk of epiphyseal fractures during closure.
Article
Cell Biology
Fumiaki Murakami, Yoriko Ando, Asuka Miyagi, Shukei Sugita, Naoto Ueno, Takeo Matsumoto
DEVELOPMENT GROWTH & DIFFERENTIATION
(2017)
Article
Engineering, Biomedical
Toshiyuki Yaguchi, Yalin Cong, Kenji Shimo, Takahiro Kurokawa, Shukei Sugita, Kazuaki Nagayama, Hiroshi Masuda, Takeo Matsumoto
ANNALS OF BIOMEDICAL ENGINEERING
(2017)
Article
Biophysics
Nathan D. Camarillo, Rafael Jimenez-Silva, Frances T. Sheehan
Summary: This article discusses the statistical dependence between multiple measurements from the same participant and provides recommendations for using these measurements when they are not independent.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
J. Huet, A. -S. Boureau, A. Sarcher, C. Cornu, A. Nordez
Summary: Standard compression in freehand 3D ultrasound induces a bias in volume calculations, but minimal compression and gel pad methods have similar results. With a trained examiner and precautions, the bias can be minimized and become acceptable in clinical applications.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
C. Lariviere, A. H. Eskandari, H. Mecheri, F. Ghezelbash, D. Gagnon, A. Shirazi-Adl
Summary: Recent developments in musculoskeletal modeling have focused on model customization. Personalization of the spine profile may affect estimates of spinal loading and stability. This study investigates the biomechanical consequences of changes in the spinal profile and finds that personalizing the spine profile has medium to large effects on trunk muscle forces and negligible to small effects on spinal loading and stability.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Luke T. Mattar, Arash B. Mahboobin, Adam J. Popchak, William J. Anderst, Volker Musahl, James J. Irrgang, Richard E. Debski
Summary: Exercise therapy fails in about 25.0% of cases for individuals with rotator cuff tears, and one reason for this failure may be the inability to strengthen and balance the muscle forces that keep the humeral head in the correct position. This study developed computational musculoskeletal models to compare the net muscle force before and after exercise therapy between successfully and unsuccessfully treated patients. The study found that unsuccessfully treated patients had less inferiorly oriented net muscle forces, which may increase the risk of impingement.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Natsuki Sado, Takeshi Edagawa, Toshihide Fujimori, Shogo Hashimoto, Yoshikazu Okamoto, Takahito Nakajima
Summary: The existing methods for predicting hip and lumbosacral joint centres in Japanese adults are biased and differ between sexes. We propose new regression equations that consider soft-tissue thickness, sex differences, and a height-directional measure, and validate them using leave-one-out cross-validation.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Peimin Yu, Xuanzhen Cen, Qichang Mei, Alan Wang, Yaodong Gu, Justin Fernandez
Summary: This study aimed to explore the intra-foot biomechanical differences among individuals with chronic ankle instability (CAI), copers, and healthy individuals during dynamic tasks. The study found that copers and CAI individuals had smaller dorsiflexion angles and copers presented a more eversion position compared to healthy participants. Copers also had greater dorsiflexion angles in the metatarsophalangeal joint and more inversion moments in the subtalar joint during certain tasks. These findings can help in designing interventions to restore ankle joint functions in CAI individuals.
JOURNAL OF BIOMECHANICS
(2024)
Article
Biophysics
Jon Skovgaard Jensen, Anders Holsgaard-Larsen, Anders Stengaard Sorensen, Per Aagaard, Jens Bojsen-Moller
Summary: This study investigates the biomechanical effects of robot-assisted body weight unloading (BWU) on gait patterns in healthy young adults. The results show that dynamic robot-assisted BWU enables reduced kinetic requirements without distorting biomechanically normal gait patterns during overground walking.
JOURNAL OF BIOMECHANICS
(2024)