Article
Biochemistry & Molecular Biology
Anna Bajnok, Timea Sereny-Litvai, Viktoria Temesfoi, Jasper Norenberg, Robert Herczeg, Ambrus Kaposi, Timea Berki, Emese Mezosi
Summary: Calcium (Ca2+) flux is an important signaling pathway in B cells, and changes in flux are associated with autoimmune dysregulation and B-cell malignancies. We developed a flow-cytometry-based method to study Ca2+ flux in circulating human B lymphocytes from healthy individuals and found that different stimuli trigger distinct Ca2+ flux responses. Our results also showed that the Ca2+ flux response patterns differ among B-cell subsets at different developmental stages. The findings suggest that Ca2+ flux can be used as a functional test for B cells and may provide insights into pathological B-cell activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jiayu Song, Yuan Ren, Lihua Liu, Yixin Wang, Chuandao Shi, Xiaoqing Ji, Yunlan Li, Qingshan Li
Summary: This study reveals that Di-n-butyl-di-(4-chlorobenzohydroxamato) tin(IV) (DBDCT) has strong antitumor activity similar to cisplatin, but also exhibits toxicity. Proteomic analysis identifies Gal-1 as a potential target for DBDCT-induced hepatotoxicity. Additionally, DBDCT promotes ROS production, activates NF-kappa B p65, inhibits Ras and p-ERK1/2 expressions, and modulates the expressions of several proteins related to apoptosis.
BIOINORGANIC CHEMISTRY AND APPLICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Ignacio Diaz-Franulic, Christian Verdugo, Felipe Gonzalez, Fernando Gonzalez-Nilo, Ramon Latorre
Summary: Living organisms rely on detecting environmental thermal cues for survival, with mammals having a complex system of Transient Receptor Potential ion channels that are controlled by various factors. The thermoTRP channels subgroup exhibits unique temperature dependence, and understanding the temperature-driven gating mechanisms remains a challenge.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Biochemistry & Molecular Biology
Federico M. Ruiz, Francisco J. Medrano, Anna-Kristin Ludwig, Herbert Kaltner, Nadezhda V. Shilova, Nicolai V. Bovin, Hans-Joachim Gabius, Antonio Romero
Summary: This study presents the first crystallographic information on the N-terminal extension of rat galectin-5, revealing the structured organization around the central Trp77 of the carbohydrate recognition domain. The ligand-bound structure demonstrates a shift in the amino acid alignment, potentially acting as a molecular switch for new contacts in this region.
Article
Biochemistry & Molecular Biology
Fei Fei, Mingfeng Zhang, Somayeh S. Tarighat, Eun Ji Joo, Lu Yang, Nora Heisterkamp
Summary: Galectin-1 and Galectin-3 have a complicated joint contribution to BCP-ALL survival, with stromal Galectin-3 playing an important role in maintaining BCP-ALL fitness during chemotherapy exposure, while stromal Galectin-1 does not significantly contribute to drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Stephanie J. Hanna, Danijela Tatovic, Terri C. Thayer, Colin M. Dayan
Summary: In recent years, significant progress has been made in the analysis of individual cells using RNA sequencing techniques, leading to exciting discoveries in the immunology of type 1 diabetes (T1D). Single-cell RNA sequencing has revealed specific gene expression patterns in T1D patients, allowing for predictions of disease development and offering insights into potential immunotherapy targets for T1D.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Xizhi Yu, Junjie Qian, Limin Ding, Shengyong Yin, Lin Zhou, Shusen Zheng
Summary: Galectin-1, a 14-kDa protein that interacts with carbohydrates, plays a crucial role in pathogen recognition and immune response. It has been shown to have immunosuppressive properties, but recent studies have also suggested its pro-inflammatory role in certain diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Zhiwen Shen, Hui Xu, Wen Song, Chuwen Hu, Mingyan Guo, Jinfeng Li, Junhua Li
Summary: The study showed that galectin-1 attenuated cognitive dysfunction induced by surgery in aged mice by inhibiting microglial activity and reducing inflammatory protein expression. Galectin-1 also prevented neuronal loss in the hippocampus and inhibited inflammation in microglial cells, suggesting its potential protective effects on surgery-induced neuroinflammation.
CNS NEUROSCIENCE & THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Brady A. Travis, Kathryn M. Ramsey, Samantha M. Prezioso, Thomas Tallo, Jamie M. Wandzilak, Allen Hsu, Mario Borgnia, Alberto Bartesaghi, Simon L. Dove, Richard G. Brennan, Maria A. Schumacher
Summary: This study reveals a unique mechanism for Ft pathogenesis involving a virulence-specialized RNAP that employs two (MglA-SspA)-based strategies to activate virulence genes.
Article
Biology
Fenghui Zhao, Chao Zhang, Qingtong Zhou, Kaini Hang, Xinyu Zou, Yan Chen, Fan Wu, Qidi Rao, Antao Dai, Wanchao Yin, Dan-Dan Shen, Yan Zhang, Tian Xia, Raymond C. Stevens, H. Eric Xu, Dehua Yang, Lihua Zhao, Ming-Wei Wang
Summary: This study presents the cryo-EM structure of human GIPR in complex with GIP and a G(s) trimer, revealing a structural framework of hormone recognition and GIPR activation. It shows the specific interactions between GIP and GIPR that contribute to their metabolic functions.
Article
Immunology
Maureen Banach, Isaac T. W. Harley, Andrew Getahun, John C. Cambier
Summary: The presence of high-affinity insulin-reactive B cells is associated with the development of autoimmune type 1 diabetes. These B cells show specific phenotypes and express unmutated immunoglobulin light chains that are biased towards certain genes. The relaxation of tolerance mechanisms in NOD mice leads to the accumulation and partial activation of B cells expressing high-affinity B cell receptors, contributing to the development of autoimmunity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Nathanael A. Caveney, Robert A. Saxton, Deepa Waghray, Caleb R. Glassman, Naotaka Tsutsumi, Stevan R. Hubbard, Christopher Garcia
Summary: Janus kinases (JAKs) play a key role in cytokine signaling by mediating the transduction of signals downstream of cytokine receptors. The cryoelectron microscopy structure of a mouse JAK1 complex provides insights into the trans-activation step of JAK signaling and the allosteric mechanisms of JAK inhibition.
Article
Biochemistry & Molecular Biology
Zak Vilen, Eugene Joeh, Meg Critcher, Christopher G. Parker, Mia L. Huang
Summary: Galectin-1 is a key protein in promoting myogenic differentiation, but the glycoprotein interactors of galectin-1 are not fully understood. A proximity tagging strategy has been used to capture and identify the glycan-mediated glycoprotein interactors of galectin-1 in myoblasts, providing valuable resources for studying the mechanisms through which galectin-1 promotes myogenic differentiation.
ACS CHEMICAL BIOLOGY
(2021)
Review
Immunology
Yuhan Sun, Xueying Li, Tiantong Wang, Wenzhe Li
Summary: This review aims to discuss the importance of core fucosylation in immune response, including its role in protein conformation, signal transduction, and cell-cell interactions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Nairie Michaelian, Anastasiia Sadybekov, Elie Besserer-Offroy, Gye Won Han, Harini Krishnamurthy, Beata A. Zamlynny, Xavier Fradera, Phieng Siliphaivanh, Jeremy Presland, Kerrie B. Spencer, Stephen M. Soisson, Petr Popov, Philippe Sarret, Vsevolod Katritch, Vadim Cherezov
Summary: The leukotriene B4 receptor 1 (BLT1) plays a role in various human diseases and the crystal structure study reveals molecular determinants of ligand binding and selectivity toward different receptor subtypes, providing insights for future structure-based drug design.
NATURE COMMUNICATIONS
(2021)
Article
Hematology
Nagham Alouche, Amelie Bonaud, Vincent Rondeau, Rim Hussein-Agha, Julie Nguyen, Valeria Bisio, Melanie Khamyath, Etienne Crickx, Niclas Setterblad, Nicolas Dulphy, Matthieu Mahevas, David H. McDermott, Philip M. Murphy, Karl Balabanian, Marion Espeli
Summary: Enhanced Cxcr4 signaling due to defective receptor desensitization leads to exacerbated extrafollicular B-cell response, as demonstrated in a mouse model bearing a gain-of-function mutation of Cxcr4 found in human hematologic disorders. Mutant B cells exhibit enhanced mechanistic target of rapamycin signaling, cycle more, and differentiate more potently into plasma cells after Toll-like receptor stimulation. Additionally, Cxcr4 gain of function promotes enhanced homing and persistence of immature plasma cells in the bone marrow, resulting in increased and more sustained antibody production after T-independent immunization.
Editorial Material
Immunology
Patricia Ame, Helene Dumortier, Marion Espeli, Pierre Milpied, Nicolas Fazilleau
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Letter
Oncology
Lin-Pierre Zhao, Maxime Boy, Celia Azoulay, Emmanuelle Clappier, Marie Sebert, Ludivine Amable, Jihene Klibi, Kamel Benlagha, Marion Espeli, Karl Balabanian, Claude Preudhomme, Alice Marceau-Renaut, Lina Benajiba, Raphael Itzykson, Arsene Mekinian, Olivier Fain, Antoine Toubert, Pierre Fenaux, Nicolas Dulphy, Lionel Ades
Article
Immunology
Catherine Ouk, Lilian Roland, Nathalie Gachard, Stephanie Poulain, Christelle Oblet, David Rizzo, Alexis Saintamand, Quentin Lemasson, Claire Carrion, Morgane Thomas, Karl Balabanian, Marion Espeli, Marie Parrens, Isabelle Soubeyran, Melanie Boulin, Nathalie Faumont, Jean Feuillard, Christelle Vincent-Fabert
Summary: Activating mutations of MYD88, particularly MYD88(L265P), are commonly found in Waldenstrom macroglobulinemia and aggressive B-cell lymphomas. A mouse model expressing MYD88(L252P) in B-cells showed expansion of IgM B and plasma cells, leading to spleen enlargement and lymphoma, potentially offering new therapeutic targets.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Amelie Bonaud, Julia P. Lemos, Marion Espeli, Karl Balabanian
Summary: This review explores the bone marrow niches in mice that support hematopoietic cells, focusing on the roles of multipotent progenitors and plasma cells. The chemokine CXCL12/CXCR4 signaling axis is highlighted for its importance in these processes. The question of whether multipotent progenitors and plasma cells are neighbors or roommates within the bone marrow is raised for further exploration.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Richard Lewis, H. Carlo Maurer, Nikita Singh, Irene Gonzalez-Menendez, Matthias Wirth, Markus Schick, Le Zhang, Konstandina Isaakidis, Anna Katharina Scherger, Veronika Schulze, Junyan Lu, Thorsten Zenz, Katja Steiger, Roland Rad, Leticia Quintanilla-Martinez, Marion Espeli, Karl Balabanian, Ulrich Keller, Stefan Habringer
Summary: The study reveals that hyperactivation of CXCR4 is identified as a co-driver of an aggressive lymphoma phenotype.
Correction
Immunology
Natalie Burrows, Rachael J. M. Bashford-Rogers, Vijesh J. Bhute, Ana Penalver, John R. Ferdinand, Benjamin J. Stewart, Joscelin E. G. Smith, Mukta Deobagkar-Lele, Girolamo Giudice, Thomas M. Connor, Akimichi Inaba, Laura Bergamaschi, Sam Smith, Maxine G. B. Tran, Evangelia Petsalaki, Paul A. Lyons, Marion Espeli, Brian J. P. Huntly, Kenneth G. C. Smith, Richard J. Cornall, Menna R. Clatworthy, Patrick H. Maxwell
Review
Immunology
Celine Delaloy, Wolfgang Schuh, Hans-Martin Jaeck, Amelie Bonaud, Marion Espeli
Summary: Plasma cells were traditionally seen as a homogeneous population dedicated to antibody secretion, but recent studies have revealed phenotypic and functional heterogeneity in this cell type. Advances in single cell technologies have shed light on the factors influencing the fate and diversity of plasma cells, contributing to a better understanding of their roles in immune responses and diseases.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Nicolas Houde, Laurent Beuret, Amelie Bonaud, Simon-Pierre Fortier-Beaulieu, Kim Truchon-Landry, Rifdat Aoidi, Emilie Pic, Nagham Alouche, Vincent Rondeau, Geraldine Schlecht-Louf, Karl Balabanian, Marion Espeli, Jean Charron
Summary: This study reveals the critical role of fine-tuning the ERK/MAPK pathway in immune cell activation and function. It also demonstrates that MEK1 and MEK2 isoforms play distinct roles in lymphocyte activation and disease development.
Editorial Material
Medicine, Research & Experimental
Nicolas Houde, Marion Espeli, Jean Charron
M S-MEDECINE SCIENCES
(2022)
Article
Immunology
Amelie Bonaud, Melanie Khamyath, Marion Espeli
Summary: Plasma cells and their antibodies play a crucial role in protection against infection, but can also be involved in diseases such as autoimmune diseases and multiple myeloma. The differentiation and survival of plasma cells are regulated by transcriptional switches and morphological changes. Although some cellular and molecular mechanisms have been identified, the mechanisms by which plasma cells survive and secrete large quantities of antibodies for extended periods of time remain unclear. This review aims to discuss the current understanding of plasma cell cellular biology, the challenges faced by research in this field, and the potential opportunities for studying the fundamental mechanisms underlying plasma cell survival and function.
IMMUNOLOGY LETTERS
(2023)
Article
Medicine, Research & Experimental
Melanie Khamyath, Amelie Bonaud, Karl Balabanian, Marion Espeli
Summary: CXCR4 plays a crucial role in cell migration and the development of the immune system. It is involved in B lymphocyte biology from their differentiation to plasma cell formation. Mutations in CXCR4 can lead to a rare immunodeficiency called the WHIM Syndrome, affecting receptor desensitization and increasing response to its ligand CXCL12. This review emphasizes the regulatory role of CXCR4 desensitization in humoral immune responses, using the WHIM Syndrome as an example, and highlights the importance of fine-tuning CXCR4 signaling for long-term antibody-mediated protection.
M S-MEDECINE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Amelie Bonaud, Laetitia Gargowitsch, Simon M. Gilbert, Elanchezhian Rajan, Pablo Canales-Herrerias, Daniel Stockholm, Nabila F. Rahman, Mark O. Collins, Hakan Taskiran, Danika L. Hill, Andres Alloatti, Nagham Alouche, Stephanie Balor, Vanessa Soldan, Daniel Gillet, Julien Barbier, Francoise Bachelerie, Kenneth G. C. Smith, Julia Jellusova, Pierre Bruhns, Sebastian Amigorena, Karl Balabanian, Michelle A. Linterman, Andrew A. Peden, Marion Espeli
Summary: We identified SNARE Sec22b as a critical regulator of plasma cell maintenance and function. In the absence of Sec22b, plasma cells were hardly detectable and serum antibody titers were dramatically reduced, leading to a failure in mounting a protective immune response. Mechanistically, Sec22b contributes to efficient antibody secretion and is involved in regulating plasma cell transcriptional identity, as well as the morphology of the endoplasmic reticulum and mitochondria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Immunology
Alyssa Silva-Cayetano, Sigrid Fra-Bido, Philippe A. Robert, Silvia Innocentin, Alice R. Burton, Emily M. Watson, Jia Le Lee, Louise M. C. Webb, William S. Foster, Ross C. J. McKenzie, Alexandre Bignon, Ine Vanderleyden, Dominik Alterauge, Julia P. Lemos, Edward J. Carr, Danika L. Hill, Isabella Cinti, Karl Balabanian, Dirk Baumjohann, Marion Espeli, Michael Meyer-Hermann, Alice E. Denton, Michelle A. Linterman
Summary: Linterman and colleagues find that dysregulated CXCR4 expression in aged T-FH cells leads to their mislocation in germinal centers, impairing their ability to support B cell help and antibody production. The decline in the germinal center response with age results in poor vaccine-induced immunity in older individuals. However, this age-dependent defect can be reversed by providing T-FH cells that colocalize with follicular dendritic cells using CXCR5, highlighting the importance of T-FH cell localization in the antibody response and expansion of the FDC network.
Article
Immunology
Amelie Bonaud, Pierre Larraufie, Melanie Khamyath, Ugo Szachnowski, Shaun M. Flint, Nadege Brunel-Meunier, Francois Delhommeau, Annie Munier, Tapio Loennberg, Claire Toffano-Nioche, Daniel Gautheret, Karl Balabanian, Marion Espeli
Summary: Using single cell RNAseq and in silico transinteractome analyses, the researchers identified Leptin receptor positive (LepR(+)) mesenchymal cells as the stromal cell subset most likely to interact with long-lived plasma cells (PCs) within the bone marrow. Furthermore, they found that PCs may use different sets of integrins and adhesion molecules to interact with these stromal cells based on their isotype. These findings provide new insights into the specific targeting of bone marrow PCs.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)