Article
Oncology
Irene Dogliotti, Cristina Jimenez, Marzia Varettoni, Dipti Talaulikar, Tina Bagratuni, Martina Ferrante, Jose Perez, Daniela Drandi, Noemi Puig, Milena Gilestro, Maria Garcia-Alvarez, Roger Owen, Wojciech Jurczak, Alessandra Tedeschi, Veronique Leblond, Efstathios Kastritis, Marie Jose Kersten, Shirley D'Sa, Michal Kascak, Wolfgang Willenbacher, Aldo M. Roccaro, Stephanie Poulain, Pierre Morel, Charalampia Kyriakou, Falko Fend, Josephine M. Vos, Meletios A. Dimopoulos, Christian Buske, Simone Ferrero, Ramon Garcia-Sanz
Summary: This paper presents consensus recommendations and laboratory requirements for the diagnosis of Waldenstrom's macroglobulinemia (WM) developed by the European Consortium of Waldenstrom's Macroglobulinemia, providing guidance for clinicians and researchers on multiparametric flow cytometry, fluorescence in situ hybridization, and molecular tests.
Article
Hematology
Madeleine R. Berendsen, Diede A. G. van Bladel, Eva Hesius, Cristina Berganza Irusquieta, Jos Rijntjes, Annemiek B. van Spriel, Ellen van der Spek, Johannes F. M. Pruijt, Leonie I. Kroeze, Konnie M. Hebeda, Sandra Croockewit, Wendy B. C. Stevens, J. Han J. M. van Krieken, Patricia J. T. A. Groenen, Michiel van den Brand, Blanca Scheijen
Summary: Patients with lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (LPL/WM) occasionally develop diffuse large B-cell lymphoma (DLBCL), and the clonal B-cell dynamics and mutational landscape vary in these patients. About one-quarter of LPL/WM patients develop clonally unrelated DLBCL, suggesting multiple mechanisms of transformation.
Article
Multidisciplinary Sciences
Sara Rodriguez, Jon Celay, Ibai Goicoechea, Cristina Jimenez, Cirino Botta, Maria-Jose Garcia-Barchino, Juan-Jose Garces, Marta Larrayoz, Susana Santos, Diego Alignani, Amaia Vilas-Zornoza, Cristina Perez, Sonia Garate, Sarai Sarvide, Aitziber Lopez, Hans-Christian Reinhardt, Yolanda R. Carrasco, Isidro Sanchez-Garcia, Maria-Jose Larrayoz, Maria-Jose Calasanz, Carlos Panizo, Felipe Prosper, Jose-Maria Lamo-Espinosa, Marina Motta, Alessandra Tucci, Antonio Sacco, Massimo Gentile, Sara Duarte, Helena Vitoria, Catarina Geraldes, Artur Paiva, Noemi Puig, Ramon Garcia-Sanz, Aldo M. Roccaro, Gema Fuerte, Jesus F. San Miguel, Jose-Angel Martinez-Climent, Bruno Paiva
Summary: Normal B lymphocytes can accumulate mutations before lymphoma and MYD88(L265P) is a preneoplastic event. This study uncovers genetic and transcriptional pathways driving malignant transformation and models lymphoplasmacytic lymphoma in mice.
Article
Hematology
Maaya Awata-Shiraiwa, Akihiko Yokohama, Yukihiro Kanai, Nanami Gotoh, Tetsuhiro Kasamatsu, Hiroshi Handa, Takayuki Saitoh, Hirokazu Murakami, Junko Hirato, Hayato Ikota, Norifumi Tsukamoto
Summary: Using targeted next-generation sequencing (NGS), this study found no significant genetic differences between Waldenstrom macroglobulinemia (WM) and non-IgM-type lymphoplasmacytic lymphoma (LPL). These findings suggest genetic similarities between these two subsets of LPL.
ACTA HAEMATOLOGICA
(2023)
Article
Hematology
Lena Brandefors, Birgitta Sander, Kristina Lundqvist, Eva Kimby
Summary: The study revealed differences in clinical features between non-WM LPL and WM patients, such as age, prognostic factors, etc., but no significant difference in survival rate.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Jun Hee Lim, James Q. Wang, Fiona Webb, Kartik Saxena, Daniel Enosi Tuipulotu, Abhimanu Pandey, Si Ming Man, Dipti Talaulikar
Summary: Waldenstrom macroglobulinemia (WM) is characterized by the presence of a specific mutation in both malignant plasma cells (PCs) and lymphoplasmacytic cells (LPCs), and the expression of the same auto-reactive IgHV sequences in both cell types. Malignant PCs are primarily responsible for the secretion of IgM, and targeting these cells may be beneficial in the treatment of WM.
Article
Oncology
Rie Furuta, Hiro Tatetsu, Jun-ichirou Yasunaga, Mitsunori Ueno, Kento Oshiro, Satoshi Kumanomido, Yawara Kawano, Yusuke Higuchi, Yumi Honda, Yoshiki Mikami, Kisato Nosaka, Masao Matsuoka
Summary: This is a rare case in which a patient presented with weight loss, hyperproteinemia, and anemia. Further examination revealed features of both Waldenstrom's macroglobulinemia and extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) lymphoma.
LEUKEMIA RESEARCH REPORTS
(2023)
Article
Pathology
Haiyan Lu, Lisa Durkin, Xiaoxian Zhao, Megan O. Nakashima
Summary: This study characterizes the clinicopathologic features of IgM plasma cell myeloma (IgMPCM), including MYD88 L265P and CXCR4 mutations. Distinguishing IgMPCM from other IgM-related disorders requires correlation with clinical, laboratory, and radiologic findings.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Oncology
Jialing Xie, Xia Shen, Qin Shi, Hongmei Yi, Binshen Ouyang, Zhihan Zhang, Yijin Gu, Lei Dong
Summary: MYD88 mutation has a significant impact on the prognosis of DLBCL patients. Compared with L265P mutation, non-L265P mutations occur less frequently in DLBCL of the central nervous system and breast tissue and have a lower coexistence rate with PIM1 mutation. Patients with DLBCL with MYD88 non-L265P mutation have a statistically better progression-free survival.
HEMATOLOGICAL ONCOLOGY
(2022)
Article
Hematology
Madeleine R. Berendsen, Diede A. G. van Bladel, Eva Hesius, Cristina Berganza Irusquieta, Jos Rijntjes, Annemiek B. van Spriel, Ellen van der Spek, Johannes F. M. Pruijt, Leonie I. Kroeze, Konnie M. Hebeda, Sandra Croockewit, Wendy B. C. Stevens, J. Han J. M. van Krieken, Patricia J. T. A. Groenen, Michiel van den Brand, Blanca Scheijen
Summary: Patients with lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia (LPL/WM) may develop diffuse large B-cell lymphoma (DLBCL), and the clonal dynamics of B-cells during transformation vary among patients. DLBCL can be clonally related or unrelated to the major malignant B-cell clone in LPL. Commonly mutated genes are observed in DLBCL transformation.
Review
Oncology
Francesco Piazza, Veronica Di Paolo, Greta Scapinello, Sabrina Manni, Livio Trentin, Luigi Quintieri
Summary: LPL is a rare subtype of B cell-derived non-Hodgkin lymphoma characterized by abnormal growth of transformed clonal lymphoplasmacytes and plasma cells. Despite progress in therapy, LPL is almost invariably incurable and exhibits a propensity towards development of therapy resistance. This review aims to describe the clinical and pathobiological features of LPL and analyze the mechanisms of drug resistance in this disease.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Emanuela Esposito, Valeria Varone, Claudio Siani, Maurizio Di Bonito, Maria Teresa Melucci, Ivana Donzelli, Raimondo di Giacomo, Ugo Marone, Ruggero Saponara, Francesca Collina, Alfredo Fucito, Franca Avino
Summary: This case report describes a 60-year-old female with a personal and familial history of monoclonal gammopathy of undetermined significance (MGUS) and a lump in her right breast. Biopsy revealed lymphoplasmacytic lymphoma (LPL) of the breast, leading to a diagnosis of Waldenstrom's Macroglobulinemia (WM). Breast lymphoma is a rare disease often misdiagnosed due to lack of specific features on mammogram and ultrasound, making core biopsy crucial for diagnosis.
TRANSLATIONAL CANCER RESEARCH
(2023)
Review
Oncology
Ramon Garcia-Sanz, Cristina Jimenez
Summary: This review outlines the significant applications of single-cell technologies in hematological tumors, demonstrating their potential in understanding cellular heterogeneity and improving treatment strategies. By using a specific example of Waldenstrom's macroglobulinemia, the power of single-cell multi-omics in unraveling complex biology of heterogeneous populations is highlighted. Studies show that single-cell sequencing techniques can overcome the limitations of bulk sequencing, providing insights into tumor development stages and therapy resistance mechanisms.
Letter
Oncology
Yu Qiu, Xiao-shuang Wang, Yuan Yao, Yan-min Si, Xue-zhu Wang, Ming-nan Jia, Dao-bin Zhou, Jia Yu, Xin-xin Cao, Jian Li
Summary: Through single-cell RNA sequencing, we found that IgM MGUS and WM patients had fewer early B-cell subsets and more T cells and NK cells in their samples. Mature B cells of WM patients showed upregulation of several genes compared to IgM MGUS patients. We also identified a subpopulation of CD3 + CD19 + cells, which may have stemness features. Additionally, we revealed the communication pathways between CD3 + CD19 + cells and other immune cells.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2023)
Article
Oncology
Kathryn A. F. Kline, Seung Tae Lee, Jennie Y. Law, Michael Kallen
Summary: This is an article published in Clinical Lymphoma, Myeloma and Leukemia, Vol. 22, No. 8, e788???e791, 2022 Elsevier Inc.
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2022)
Article
Medical Laboratory Technology
Mylene Gayet, Vincent Leymarie, Paco Derouault, Estelle Guerin, Julien Vaidie, Virginie Pascal, Melanie Boulin, Nataliya Dmytruk, Jasmine Chauzeix, Franck Trimoreau, Nathalie Gachard, Jean Feuillard, David Rizzo
Summary: An original and simple flow cytometry analysis method proposed in this study successfully identified the ongoing plasma cell differentiation characteristic of Waldenstrom tumor B-cells, aiding in the distinction between different B-cell malignancies. The continuum of CD138 expression observed between monotypic B-cells and plasma cells was significantly associated with higher IgM peaks and MYD88 mutations, suggesting its potential as an objective marker for diagnosis.
CYTOMETRY PART B-CLINICAL CYTOMETRY
(2022)
Review
Immunology
Celine Delaloy, Wolfgang Schuh, Hans-Martin Jaeck, Amelie Bonaud, Marion Espeli
Summary: Plasma cells were traditionally seen as a homogeneous population dedicated to antibody secretion, but recent studies have revealed phenotypic and functional heterogeneity in this cell type. Advances in single cell technologies have shed light on the factors influencing the fate and diversity of plasma cells, contributing to a better understanding of their roles in immune responses and diseases.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Allergy
Anne Marchalot, Catherine Horiot, Jean-Marie Lambert, Claire Carrion, Christelle Oblet, Justine Pollet, Michel Cogne, Jeanne Moreau, Brice Laffleur, Laurent Delpy
Summary: This study demonstrates the effectiveness of an antisense oligonucleotide (ASO) strategy in reducing IgE secretion and promoting allergen tolerance by inducing apoptosis of IgE1 antibody-secreting cells.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Cell Biology
Nicolas Houde, Laurent Beuret, Amelie Bonaud, Simon-Pierre Fortier-Beaulieu, Kim Truchon-Landry, Rifdat Aoidi, Emilie Pic, Nagham Alouche, Vincent Rondeau, Geraldine Schlecht-Louf, Karl Balabanian, Marion Espeli, Jean Charron
Summary: This study reveals the critical role of fine-tuning the ERK/MAPK pathway in immune cell activation and function. It also demonstrates that MEK1 and MEK2 isoforms play distinct roles in lymphocyte activation and disease development.
Editorial Material
Medicine, Research & Experimental
Nicolas Houde, Marion Espeli, Jean Charron
M S-MEDECINE SCIENCES
(2022)
Article
Immunology
Amelie Bonaud, Melanie Khamyath, Marion Espeli
Summary: Plasma cells and their antibodies play a crucial role in protection against infection, but can also be involved in diseases such as autoimmune diseases and multiple myeloma. The differentiation and survival of plasma cells are regulated by transcriptional switches and morphological changes. Although some cellular and molecular mechanisms have been identified, the mechanisms by which plasma cells survive and secrete large quantities of antibodies for extended periods of time remain unclear. This review aims to discuss the current understanding of plasma cell cellular biology, the challenges faced by research in this field, and the potential opportunities for studying the fundamental mechanisms underlying plasma cell survival and function.
IMMUNOLOGY LETTERS
(2023)
Article
Medicine, Research & Experimental
Melanie Khamyath, Amelie Bonaud, Karl Balabanian, Marion Espeli
Summary: CXCR4 plays a crucial role in cell migration and the development of the immune system. It is involved in B lymphocyte biology from their differentiation to plasma cell formation. Mutations in CXCR4 can lead to a rare immunodeficiency called the WHIM Syndrome, affecting receptor desensitization and increasing response to its ligand CXCL12. This review emphasizes the regulatory role of CXCR4 desensitization in humoral immune responses, using the WHIM Syndrome as an example, and highlights the importance of fine-tuning CXCR4 signaling for long-term antibody-mediated protection.
M S-MEDECINE SCIENCES
(2023)
Article
Multidisciplinary Sciences
Amelie Bonaud, Laetitia Gargowitsch, Simon M. Gilbert, Elanchezhian Rajan, Pablo Canales-Herrerias, Daniel Stockholm, Nabila F. Rahman, Mark O. Collins, Hakan Taskiran, Danika L. Hill, Andres Alloatti, Nagham Alouche, Stephanie Balor, Vanessa Soldan, Daniel Gillet, Julien Barbier, Francoise Bachelerie, Kenneth G. C. Smith, Julia Jellusova, Pierre Bruhns, Sebastian Amigorena, Karl Balabanian, Michelle A. Linterman, Andrew A. Peden, Marion Espeli
Summary: We identified SNARE Sec22b as a critical regulator of plasma cell maintenance and function. In the absence of Sec22b, plasma cells were hardly detectable and serum antibody titers were dramatically reduced, leading to a failure in mounting a protective immune response. Mechanistically, Sec22b contributes to efficient antibody secretion and is involved in regulating plasma cell transcriptional identity, as well as the morphology of the endoplasmic reticulum and mitochondria.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Pathology
Estelle Bourbon, Kaddour Chabane, Isabelle Mosnier, Anne Bouvard, Florian Thonier, Emmanuelle Ferrant, Anne-Sophie Michallet, Stephanie Poulain, Sandrine Hayette, Pierre Sujobert, Sarah Huet
Summary: Current guidelines recommend determining the mutation status of the IGHV gene in CLL patients before treatment initiation. However, commercially available NGS solutions have technical constraints and high costs. This study presents a new method called Next-CLL, which allows for IGHV gene mutation status evaluation using any NGS device in routine diagnostic laboratories. Next-CLL demonstrated excellent performance and concordance with the reference technique, making it a valuable tool for assessing CLL patients.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2023)
Article
Immunology
Alyssa Silva-Cayetano, Sigrid Fra-Bido, Philippe A. Robert, Silvia Innocentin, Alice R. Burton, Emily M. Watson, Jia Le Lee, Louise M. C. Webb, William S. Foster, Ross C. J. McKenzie, Alexandre Bignon, Ine Vanderleyden, Dominik Alterauge, Julia P. Lemos, Edward J. Carr, Danika L. Hill, Isabella Cinti, Karl Balabanian, Dirk Baumjohann, Marion Espeli, Michael Meyer-Hermann, Alice E. Denton, Michelle A. Linterman
Summary: Linterman and colleagues find that dysregulated CXCR4 expression in aged T-FH cells leads to their mislocation in germinal centers, impairing their ability to support B cell help and antibody production. The decline in the germinal center response with age results in poor vaccine-induced immunity in older individuals. However, this age-dependent defect can be reversed by providing T-FH cells that colocalize with follicular dendritic cells using CXCR5, highlighting the importance of T-FH cell localization in the antibody response and expansion of the FDC network.
Article
Immunology
Nicolas Denis-Lagache, Christelle Oblet, Tiffany Marchiol, Audrey Baylet, Ophelie Teteau, Iman Dalloul, Zeinab Dalloul, Lina Zawil, Ophelie Deze, Jeanne Cook-Moreau, Alexis Saintamand, Hend Boutouil, Ahmed Amine Khamlichi, Claire Carrion, Sophie Peron, Sandrine Le Noir, Brice Laffleur, Michel Cogne
Summary: This study investigates the deletion of a specific gene structure that is associated with antibody gene variation and class switching, using a new mouse model. The results suggest that this gene deletion is associated with an increase in self-reactive antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Alexandre Perani, Sylvie Bourthoumieu, David Rizzo, Jasmine Chauzeix, Benjamin Dauriat, Pascal Turlure, Stephane Girault, Lea Veyrune, Maxime Roubinet, Jean Feuillard, Catherine Yardin, Nathalie Gachard
Summary: High-throughput sequencing is commonly used for diagnosis and prognosis, but it may identify potential germline variants that need to be confirmed on non-infiltrated tissues. Saliva is applicable for relatives, but only for 24% of patients. Collecting saliva during remission and using hair follicles as an alternative to skin biopsy are recommended.
FRONTIERS IN ONCOLOGY
(2023)
Article
Immunology
Eden Lebrault, Christelle Oblet, Keerthi Kurma, Nicolas Levoin, Robin Jeannet, Mickael Jean, Pierre Vacher, Patrick Legembre
Summary: This study investigates the stoichiometry of CD95L required for apoptotic and nonapoptotic signals. The researchers generated CD95L concatemers of different chemistries and found that a hexameric structure is best for triggering cell death, while a dimer is sufficient for inducing apoptotic response in CD95-sensitive cells. Interestingly, trimeric and hexameric forms are the only ones capable of implementing a potent Ca2+ response, indicating the requirement for both aggregation and conformation to activate the Ca2+ pathway.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Amelie Bonaud, Pierre Larraufie, Melanie Khamyath, Ugo Szachnowski, Shaun M. Flint, Nadege Brunel-Meunier, Francois Delhommeau, Annie Munier, Tapio Loennberg, Claire Toffano-Nioche, Daniel Gautheret, Karl Balabanian, Marion Espeli
Summary: Using single cell RNAseq and in silico transinteractome analyses, the researchers identified Leptin receptor positive (LepR(+)) mesenchymal cells as the stromal cell subset most likely to interact with long-lived plasma cells (PCs) within the bone marrow. Furthermore, they found that PCs may use different sets of integrins and adhesion molecules to interact with these stromal cells based on their isotype. These findings provide new insights into the specific targeting of bone marrow PCs.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Gregory Lazarian, Bernard Leroy, Floriane Theves, Myriam Hormi, Remi Letestu, Virginie Eclache, Giulia Tueur, Adam Ameur, Audrey Bidet, Pascale Cornillet-Lefebvre, Frederic Davi, Eric Delabesse, Marie-Helene Estienne, Pascaline Etancelin, Olivier Kosmider, Sophy Laibe, Marc Muller, Nathalie Nadal, Dina Naguib, Cedric Pastoret, Stephanie Poulain, Pierre Sujobert, Lauren Veronese, Samia Imache, Valerie Lefebvre, Florence Cymbalista, Fanny Baran-Marszak, Thierry Soussi
Summary: TP53 aberrations are a major predictive factor for resistance to chemoimmunotherapy in CLL. This study analyzed 1,056 TP53 variants from 683 patients in France and compared them to a dataset of 5,173 TP53 variants from published articles. The analysis identified CLL-specific hotspot mutations and a novel splice variant. The study also found frequent copy-neutral loss of heterozygosity in CLL and the presence of multiple TP53 variants in a high proportion of patients.