Review
Cell Biology
Nan Wang, Chong Wang, Hongyang Zhao, Yichun He, Beiwu Lan, Liankun Sun, Yufei Gao
Summary: The cooperation and antagonism between organelles within the cell play a crucial role in maintaining cellular homeostasis. The signaling crosstalk between the endoplasmic reticulum (ER) and mitochondria influences cell fate. Mitochondria-associated membranes (MAMs) are pivotal in regulating lipid synthesis, calcium homeostasis, ER-mitochondrial coordination, and death signal transduction, contributing to cell death mechanisms such as apoptosis.
Article
Agriculture, Multidisciplinary
Xin Li, Jiang Zhu, Qian Lin, Minjie Yu, Jianjun Lu, Jie Feng, Caihong Hu
Summary: This study found that curcumin has a protective effect, improving jejunal morphology and barrier function, enhancing mitochondrial function and ultrastructure, alleviating endoplasmic reticulum stress, and preventing excessive MAM formation and disorder.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Yu-ting Liu, Hao Zhang, Shao-bin Duan, Jian-wen Wang, Hong Chen, Ming Zhan, Wei Zhang, Ai-mei Li, Yan Liu, Yang Yang, Shikun Yang
Summary: Aims: This study aimed to investigate the regulatory effect of Mitofusin2 (Mfn2) on mitochondria-associated endoplasmic reticulum membrane (MAM) integrity and cellular injury in cisplatin-induced acute kidney injury (CP-AKI).Results: In CP-AKI mice, decreased Mfn2 expression, increased p-AMPK expression, abnormal mitochondrial morphology, reduced MAMs integrity, and activation of mitochondrial ROS and ER stress were observed. In vitro studies confirmed CP-induced mitochondrial ROS, ER-stress activation, and apoptosis accompanied by downregulation of Mfn2 and reduction in MAMs integrity. The restoration of MAMs integrity and inhibition of cell apoptosis were observed after pretreatment with Mfn2 plasmid in BUMPT cells, while Mfn2 siRNA treatment increased ER-stress and MAMs integrity violations in HK-2 cells under CP treatment. Coimmunoprecipitation analysis showed the interaction between Mfn2 and PERK/IRE1. AMP-activated protein kinase (AMPK) activation had a similar effect to Mfn2 plasmid, while ER-stress inhibitor had no effect on Mfn2 expression and MAMs integrity.Innovation and Conclusion: This study is the first to explore the association between MAMs, ER stress, and Mfn2 in CP-AKI. Downregulation of Mfn2 expression disrupted MAMs integrity, induced ER stress, mitochondrial ROS, and tubular cell apoptosis. The Mfn2-MAMs pathway may serve as a potential therapeutic target in CP-AKI.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Food Science & Technology
Chaoqun Wu, Guangwen Shu, Huiqi Huang, Kejian Pang, Xinzhou Yang, Guangzhong Yang
Summary: The chemical component methylgerambullin isolated from Glycosmis pentaphylla showed significant anti-hepatocellular carcinoma (HCC) activity by inducing mitochondrial apoptosis, activating ERS signaling pathways, and inhibiting the Akt and STAT3 pathways to inhibit HCC cell proliferation.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Junjun Peng, Xueyan Dai, Huiqin Fan, Chenghong Xing, Yu Zhuang, Xiaona Gao, Huabin Cao, Guoliang Hu, Fan Yang
Summary: Copper (Cu) at high levels can cause harm to the host physiology, specifically resulting in nephrotoxicity, although the exact mechanism remains unclear. This study explored the relationship between mitochondria-associated ER membranes (MAM) and Cu-induced kidney mitochondrial dysfunction and ER stress. The findings revealed that excessive Cu disrupted MAM integrity, leading to MAM dysfunction, mitochondrial dysfunction, and ER stress, contributing to Cu-induced nephrotoxicity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Medicine, Research & Experimental
Weiwei Yu, Haiqiang Jin, Yining Huang
Summary: Alzheimer's disease is a progressive neurodegenerative disorder, and recent studies suggest that mitochondria-associated membranes (MAMs) may play a crucial role in its pathogenesis. Understanding the role of MAMs could lead to new therapeutic strategies, shifting the focus from treatment to prevention in AD.
Article
Biochemistry & Molecular Biology
Vitali T. Cheshchevik, Nina G. Krylova, Nina G. Cheshchevik, Elena A. Lapshina, Galina N. Semenkova, Ilya B. Zavodnik
Summary: Hypochlorite induces oxidative damage to mitochondria, leading to changes in mitochondrial functional parameters and calcium release and swelling. Mitochondria play a key role in oxidant-induced injury mechanisms, mediating the redistribution of cytoplasmic Ca2+ ions and the release of calcium from the endoplasmic reticulum into the cytoplasm.
Review
Neurosciences
Jennifer H. Pham, Dorota L. Stankowska
Summary: Glaucoma is a leading cause of blindness worldwide and is commonly associated with elevated intraocular pressure. Recent studies suggest that mitochondrial dysfunction plays a crucial role in glaucomatous neurodegeneration. The retina, especially the retinal ganglion cells (RGCs), relies heavily on mitochondrial energy for signal transduction, making them vulnerable to oxidative damage. Mitochondrial dysfunction and ER stress have been observed in RGCs in glaucoma models, and the potential cross-signaling and roles of MAMs need to be further investigated.
FRONTIERS IN NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Yi Luan, Guangyu Guo, Ying Luan, Yang Yang, Ruixia Yuan
Summary: Pathological cardiac hypertrophy is a precursor to heart failure and requires precise examination of cardiomyocyte subtypes and biological processes for therapeutic approaches. The study analyzed the expression of MAM proteins in cardiac hypertrophy and found a preferential accumulation in cardiomyocytes at the initial stage, followed by a gradual decline. Differentiation trajectory and regulon modules in cardiomyocyte subtypes were identified, along with a cluster of MAM-related genes correlated with diabetic cardiomyopathy. These findings provide potential therapeutic targets for combating cardiac hypertrophy.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Yu-Shen Luo, Quan-Kuo He, Ming-Xin Sun, Feng-Xin Qiao, Yue-Cen Liu, Chang-Long Xu, Zhi-Ran Xu, Si-Cheng Zhao, Hai-Long Wang, Zhong-Quan Qi, Yu Liu
Summary: Acrylonitrile treatment disrupts ovarian homeostasis, leading to impaired follicular development and reduced offspring. This is associated with inflammation, apoptosis, DNA damage, oxidative stress, endoplasmic reticulum abnormalities, and lysosomal aggregation caused by acrylonitrile exposure.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Mohamed A. Eldeeb, Cornelia E. Zorca, Mohamed A. Ragheb, Fatma B. Rashidi, Doaa S. Salah El-Din
Summary: Autophagy plays a crucial role in maintaining cellular homeostasis through both selective and non-selective degradation, with endoplasmic reticulum autophagy (ER-phagy) being a significant subclass. ER-phagy selectively encapsulates and degrades the endoplasmic reticulum in response to stress cues, with recent studies uncovering novel regulators involving post-translational modifications and functional cross-talk with other cellular processes.
Article
Biochemistry & Molecular Biology
Jingjing Pan, Yanfeng Wei, Linlin Ni, Xiaoyang Li, Yu Deng, Bin Xu, Tianyao Yang, Jingyi Sun, Wei Liu
Summary: This study investigates the role of calcium ion (Ca2+) homeostasis in MeHg-induced neurotoxicity. The results suggest that MeHg causes cell damage and increases Ca2+ release from the ER as well as free Ca2+ levels in the cytoplasm and mitochondria. Ca2+ channel inhibitors and Ca2+ chelator can partially inhibit MeHg-induced mitochondrial damage and apoptosis.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Biology
Vivian W. R. Moraes, Vivian M. Santos, Eloah R. Suarez, Leticia S. Ferraz, Rayssa de Mello Lopes, Giuliana P. Mognol, Joana D. Campeiro, Joao A. Machado-Neto, Fabio D. Nascimento, Mirian A. F. Hayashi, Ivarne L. S. Tersariol, Donald D. Newmeyer, Tiago Rodrigues
Summary: Mitochondria play crucial roles in cellular physiology, and their dysregulation has been linked to cancer cells. Thioridazine (TR) induces cytotoxicity in human leukemia cells through a mechanism involving calcium imbalance, mitochondrial permeabilization, endoplasmic reticulum stress, and apoptosis. Additionally, TR shows potential as an adjuvant in antitumor chemotherapy.
Review
Cell Biology
Andrea Markovinovic, Jenny Greig, Sandra Maria Martin-Guerrero, Shaakir Salam, Sebastien Paillusson
Summary: Recent advances have uncovered common pathological changes in neurodegenerative diseases, with a particular focus on the role of endoplasmic reticulum (ER)-mitochondria signaling and mitochondria-associated membranes (MAMs). MAMs appear to be associated with major neurodegenerative diseases and have been found to regulate neuronal health and synaptic transmission. Damage to the ER-mitochondria axis disrupts vital processes, leading to neurodegeneration.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Yew Mun Lee, Weifeng He, Yih-Cherng Liou
Summary: Cellular ROS can drive important biological signaling pathways through redox signaling, with mitochondria and mitochondrial H2O2 potentially implicated in the pathogenesis of neurodegenerative diseases.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Enzo Calautti, Lidia Avalle, Valeria Poli
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Lidia Avalle, Annalisa Camporeale, Giampaolo Morciano, Natascia Caroccia, Elena Ghetti, Valeria Orecchia, Daniele Viavattene, Carlotta Giorgi, Paolo Pinton, Valeria Poli
CELL DEATH AND DIFFERENTIATION
(2019)
Article
Oncology
Emanuele Monteleone, Valeria Orecchia, Paola Corrieri, Davide Schiavone, Lidia Avalle, Enrico Moiso, Aurora Savino, Ivan Molineris, Paolo Provero, Valeria Poli
Editorial Material
Oncology
Emanuele Monteleone, Valeria Poli
Article
Multidisciplinary Sciences
Anna Morena D'Alise, Guido Leoni, Gabriella Cotugno, Fulvia Troise, Francesca Langone, Imma Fichera, Maria De Lucia, Lidia Avalle, Rosa Vitale, Adriano Leuzzi, Veronica Bignone, Elena Di Matteo, Fabio Giovanni Tucci, Valeria Poli, Armin Lahm, Maria Teresa Catanese, Antonella Folgori, Stefano Colloca, Alfredo Nicosia, Elisa Scarselli
NATURE COMMUNICATIONS
(2019)
Article
Medicine, Research & Experimental
Lidia Avalle, Francesca Marino, Annalisa Camporeale, Chiara Guglielmi, Daniele Viavattene, Silvio Bandini, Laura Conti, James Cimino, Marco Forni, Cristina Zanini, Alessandra Ghigo, Roman L. Bogorad, Federica Cavallo, Paolo Provero, Victor Koteliansky, Valeria Poli
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2020)
Review
Oncology
Valeria Poli, Laura Secli, Lidia Avalle
Review
Biochemistry & Molecular Biology
Laura Secli, Federica Fusella, Lidia Avalle, Mara Brancaccio
Summary: Heat shock proteins play crucial roles in cellular homeostasis and stress response, with extracellular heat shock proteins potentially contributing to cancer development and metastasis by supporting cancer hallmarks and modulating the tumor microenvironment.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Oncology
Laura Secli, Lidia Avalle, Pietro Poggio, Giuseppe Fragale, Cristiana Cannata, Laura Conti, Andrea Iannucci, Giovanna Carra, Cristina Rubinetto, Barbara Miniscalco, Emilio Hirsch, Valeria Poli, Alessandro Morotti, Marco De Andrea, Emilia Turco, Federica Cavallo, Federica Fusella, Mara Brancaccio
Summary: This study found that the HSP90 co-chaperone Morgana is released by cancer cells and induces cancer cell migration through TLR2, TLR4, and LRP1. Targeting Morgana extracellular activity may inhibit metastasis and enhance the CD8(+) T-cell-mediated antitumor immune response, suggesting therapeutic value in cancer treatment.
Article
Biochemistry & Molecular Biology
Lidia Avalle, Laura Raggi, Emanuele Monteleone, Aurora Savino, Daniele Viavattene, Luisa Statello, Andrea Camperi, Simona Aversano Stabile, Vincenzo Salemme, Niccolo De Marzo, Francesca Marino, Chiara Guglielmi, Andrea Lobascio, Cristina Zanini, Marco Forni, Danny Incarnato, Paola Defilippi, Salvatore Oliviero, Valeria Poli
Summary: In the tumor microenvironment, cancer cells activate Cancer Associated Fibroblasts (CAFs) to increase their secretion of soluble factors, which contribute to tumor cell proliferation, invasion, and dissemination. STAT3 and IL-6, as key factors, work together to maintain high levels of IL-6 secretion and STAT3 activation. The study demonstrated the essential role of STAT3 in the pro-tumorigenic functions of murine breast cancer CAFs, and identified a STAT3 signature enriched for genes encoding secreted proteins.
Article
Oncology
Antonino Di Lorenzo, Elisabetta Bolli, Roberto Ruiu, Giuseppe Ferrauto, Enza Di Gregorio, Lidia Avalle, Aurora Savino, Pietro Poggio, Irene Fiore Merighi, Federica Riccardo, Mara Brancaccio, Elena Quaglino, Federica Cavallo, Laura Conti
Summary: High expression of TLR2 is associated with poor prognosis in breast cancer patients. Using a mouse model, we demonstrate the importance of TLR2 in maintaining breast cancer stem cells and inducing regulatory T cells. TLR2 inhibitors can reduce tumor growth and enhance the efficacy of chemotherapy.
Meeting Abstract
Oncology
Anna Morena D'Alise, Guido Leoni, Gabriella Cotugno, Fulvia Troise, Francesca Langone, Imma Fichera, Maria De Lucia, Rosa Vitale, Adriano Leuzzi, Veronica Bignone, Elena Di Matteo, Fabio Giovanni Tucci, Lidia Avalle, Valeria Poli, Armin Lahm, Maria Teresa Catanese, Antonella Folgori, Stefano Colloca, Alfredo Nicosia, Elisa Scarselli
CANCER IMMUNOLOGY RESEARCH
(2019)