From Protein Engineering to Immobilization: Promising Strategies for the Upgrade of Industrial Enzymes

Structure-Based Engineering of Methionine Residues in the Catalytic Cores of Alkaline Amylase from Alkalimonas amylolytica for Improved Oxidative Stability

(2012) Haiquan Yang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improved Thermostability of Clostridium thermocellum Endoglucanase Cel8A by Using Consensus-Guided Mutagenesis

(2012) Michael Anbar et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Immobilization of the α-amylase of Bacillus amyloliquifaciens TSWK1-1 for the improved biocatalytic properties and solvent tolerance

(2012) B. A. Kikani et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Multiple amino acid substitutions significantly improve the thermostability of feruloyl esterase A from Aspergillus niger

(2012) Shuai-Bing Zhang et al.   BIORESOURCE TECHNOLOGY

Rational Co-Immobilization of Bi-Enzyme Cascades on Porous Supports and their Applications in Bio-Redox Reactions with In Situ Recycling of Soluble Cofactors

(2012) Javier Rocha-Martín et al.   ChemCatChem

A novel approach for efficient immobilization and stabilization of papain on magnetic gold nanocomposites

(2012) Banalata Sahoo et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Structure-guided mutagenesis for the improvement of substrate specificity ofBacillus megateriumglucose 1-dehydrogenase IV

(2012) Taiki Nishioka et al.   FEBS Journal

Coimmobilization of Detergent Enzymes onto a Plastic Bucket and Brush for Their Application in Cloth Washing

(2012) C. S. Pundir et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Rational Mutagenesis of Cyclodextrin Glucanotransferase at the Calcium Binding Regions for Enhancement of Thermostability

(2012) Poh Hong Goh et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Improvement of the acid stability of Bacillus licheniformis alpha amylase by error-prone PCR

(2012) Y.H. Liu et al.   JOURNAL OF APPLIED MICROBIOLOGY

Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase

(2012) Xinhao Ye et al.   Molecular BioSystems

Improving thermostability of phosphatidylinositol-synthesizing Streptomyces phospholipase D

(2012) J. Damnjanovic et al.   PROTEIN ENGINEERING DESIGN & SELECTION

An efficient method for enzyme immobilization evidenced by atomic force microscopy

(2012) C. Marcuello et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Mutations in the substrate entrance region of  -glucosidase from Trichoderma reesei improve enzyme activity and thermostability

(2012) H.-L. Lee et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Green coconut fiber: a novel carrier for the immobilization of commercial laccase by covalent attachment for textile dyes decolourization

(2012) Raquel O. Cristóvão et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Engineering a thermo-stable superoxide dismutase functional at sub-zero to >50°C, which also tolerates autoclaving

(2012) Arun Kumar et al.   Scientific Reports

Engineering Platforms for Directed Evolution of Laccase from Pycnoporus cinnabarinus

(2011) S. Camarero et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improvement in the Thermostability of d-Psicose 3-Epimerase from Agrobacterium tumefaciens by Random and Site-Directed Mutagenesis

(2011) Jin-Geun Choi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-β-d-glucanase

(2011) Jian-Wen Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Covalent immobilization of recombinant Rhizobium etli CFN42 xylitol dehydrogenase onto modified silica nanoparticles

(2011) Ye-Wang Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Glyoxyl-Disulfide Agarose: A Tailor-Made Support for Site-Directed Rigidification of Proteins

(2011) Cesar A. Godoy et al.   BIOMACROMOLECULES

Activity stabilization of Aspergillus niger and Escherichia coli phytases immobilized on allophanic synthetic compounds and montmorillonite nanoclays

(2011) Daniel Menezes-Blackburn et al.   BIORESOURCE TECHNOLOGY

The imminent role of protein engineering in synthetic biology

(2011) Jee Loon Foo et al.   BIOTECHNOLOGY ADVANCES

Potential applications of enzymes immobilized on/in nano materials: A review

(2011) Shakeel Ahmed Ansari et al.   BIOTECHNOLOGY ADVANCES

Development of thermostable Candida antarctica lipase B through novel in silico design of disulfide bridge

(2011) Quang Anh Tuan Le et al.   BIOTECHNOLOGY AND BIOENGINEERING

Rationally selected single-site mutants of the Thermoascus aurantiacus endoglucanase increase hydrolytic activity on cellulosic substrates

(2011) Sneha Srikrishnan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enhancement of pH stability and activity of glycerol dehydratase from Klebsiella pneumoniae by rational design

(2011) Xianghui Qi et al.   BIOTECHNOLOGY LETTERS

Substitution of Asp189 residue alters the activity and thermostability of Geobacillus sp. NTU 03 lipase

(2011) Tsung-Wei Shih et al.   BIOTECHNOLOGY LETTERS

Improvement of the thermostability and enzymatic activity of cholesterol oxidase by site-directed mutagenesis

(2011) Yan Sun et al.   BIOTECHNOLOGY LETTERS

Construction and characterization of a fusion β-1,3-1,4-glucanase to improve hydrolytic activity and thermostability

(2011) Juntao Sun et al.   BIOTECHNOLOGY LETTERS

Co-immobilization of three cellulases on Au-doped magnetic silicananoparticles for the degradation of cellulose

(2011) Eun Jin Cho et al.   CHEMICAL COMMUNICATIONS

Enhancement of a bacterial laccase thermostability through directed mutagenesis of a surface loop

(2011) Nasrin Mollania et al.   ENZYME AND MICROBIAL TECHNOLOGY

Engineering of a metagenome derived lipase toward thermal tolerance: Effect of asparagine to lysine mutation on the protein surface

(2011) Pushpender Kumar Sharma et al.   GENE

Silver nanoparticle (AgNPs) doped gum acacia–gelatin–silica nanohybrid: An effective support for diastase immobilization

(2011) Vandana Singh et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate

(2011) Juan M. Bolivar et al.   JOURNAL OF BIOTECHNOLOGY

Shape reformable polymeric nanofibers entrapped with QDs as a scaffold for enzyme stabilization

(2011) Ee Taek Hwang et al.   JOURNAL OF MATERIALS CHEMISTRY

Improvement in the Catalytic Activity of β-Agarase AgaA from Zobellia galactanivorans by Site-Directed Mutagenesis

(2011) Seungwoo Lee   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Peptide-Modified Surfaces for Enzyme Immobilization

(2011) Jinglin Fu et al.   PLoS One

Engineering highly thermostable xylanase variants using an enhanced combinatorial library method

(2011) C. A. Hokanson et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Stabilization of a raw starch digesting amylase from Aspergillus carbonarius via immobilization on activated and non-activated agarose gel

(2011) Tochukwu N. Nwagu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Improving the Catalytic Activity of HyperthermophilicPyrococcus horikoshiiProlidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

(2011) Casey M. Theriot et al.   ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL

Fabrication and kinetic studies of a novel silver nanoparticles–glucose oxidase bioconjugate

(2010) N. Hashemifard et al.   ANALYTICA CHIMICA ACTA

Covalent immobilization of β-1,4-glucosidase from Agaricus arvensis onto functionalized silicon oxide nanoparticles

(2010) Raushan Kumar Singh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cloning and characterization of a thermostable xylitol dehydrogenase from Rhizobium etli CFN42

(2010) Manish Kumar Tiwari et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhanced activity and stability of l-arabinose isomerase by immobilization on aminopropyl glass

(2010) Ye-Wang Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Mutation of outer-shell residues modulates metal ion co-ordination strength in a metalloenzyme

(2010) Jee-Loon Foo et al.   BIOCHEMICAL JOURNAL

Enhanced thermal and ultrasonic stability of a fungal protease encapsulated within biomimetically generated silicate nanospheres

(2010) Ashkan Madadlou et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Kinetic studies of Gly28:Ser mutant form of Bacillus pumilus lipase: Changes in kcat and thermal dependence

(2010) Ismael Bustos-Jaimes et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Improving the thermostability of Geobacillus stearothermophilus xylanase XT6 by directed evolution and site-directed mutagenesis

(2010) Zhi-Gang Zhang et al.   BIORESOURCE TECHNOLOGY

Immobilization of horseradish peroxidase on nanoporous copper and its potential applications

(2010) Huajun Qiu et al.   BIORESOURCE TECHNOLOGY

Enhancement of the thermostability of the maltogenic amylase MAUS149 by Gly312Ala and Lys436Arg substitutions

(2010) Sameh Ben Mabrouk et al.   BIORESOURCE TECHNOLOGY

Stable and efficient immobilization technique of aldolase under consecutive microwave irradiation at low temperature

(2010) Anming Wang et al.   BIORESOURCE TECHNOLOGY

Three amino acid changes contribute markedly to the thermostability of β-glucosidase BglC from Thermobifida fusca

(2010) Xiao-Qiong Pei et al.   BIORESOURCE TECHNOLOGY

Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum

(2010) Zhuo-Lin Yi et al.   BIORESOURCE TECHNOLOGY

Immobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl ester

(2010) Elif Yilmaz et al.   BIORESOURCE TECHNOLOGY

Thermostable Variants of Pyranose 2-Oxidase Showing Altered Substrate Selectivity for Glucose and Galactose

(2010) Oliver Spadiut et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Immobilization of Cellulase on a Reversibly Soluble−Insoluble Support: Properties and Application

(2010) Jianqin Zhou   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Engineering of protease-resistant phytase from Penicillium sp.: High thermal stability, low optimal temperature and pH

(2010) Qianqian Zhao et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Calcium alginate entrapped preparation of α-galactosidase: its stability and application in hydrolysis of soymilk galactooligosaccharides

(2010) S. K. Shankar et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Improving a Natural Enzyme Activity through Incorporation of Unnatural Amino Acids

(2010) Isaac N. Ugwumba et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Comparison of Covalent Immobilization and Physical Adsorption of a Cellulase Enzyme Mixture

(2010) S. L. Hirsh et al.   LANGMUIR

Directed evolution of CotA laccase for increased substrate specificity using Bacillus subtilis spores

(2010) Nirupama Gupta et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Structure-Based Rational Design of a Phosphotriesterase

(2009) C. J. Jackson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Immobilization of Modified Papain with Anhydride Groups on Activated Cotton Fabric

(2009) Yong Xue et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enantioselective hydrolysis of (R/S)-Naproxen methyl ester with sol–gel encapculated lipase in presence of calix[n]arene derivatives

(2009) Ozlem Sahin et al.   APPLIED CATALYSIS A-GENERAL

β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production

(2009) Sang-Mok Lee et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Immobilized invertase

(2009) S.M. Kotwal et al.   BIOTECHNOLOGY ADVANCES

Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis

(2009) Chuan-Qi Zhong et al.   BIOTECHNOLOGY AND BIOENGINEERING

A thermostable triple mutant of pyranose 2-oxidase fromTrametes multicolorwith improved properties for biotechnological applications

(2009) Oliver Spadiut et al.   Biotechnology Journal

Selective Covalent Protein Immobilization: Strategies and Applications

(2009) Lu Shin Wong et al.   CHEMICAL REVIEWS

Protein structure prediction: when is it useful?

(2009) Yang Zhang   CURRENT OPINION IN STRUCTURAL BIOLOGY

Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution

(2009) Eugene Rha et al.   FEBS Journal

Engineering thermal stability of l-asparaginase by in vitro directed evolution

(2009) Georgia A. Kotzia et al.   FEBS Journal

Microwave-assisted immobilization of lipase fromCandida rugosaon Eupergit®supports

(2009) Dejan Bezbradica et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Synthesis of magnetic/silicananoparticles with a core of magnetic clusters and their application for the immobilization of His-tagged enzymes

(2009) EunJi Woo et al.   JOURNAL OF MATERIALS CHEMISTRY

Preparation of nanoparticles which contains histidine for immobilization of Trametes versicolor laccase

(2009) M. Emin Çorman et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Incorporation of unnatural amino acids for synthetic biology

(2009) Natalya Voloshchuk et al.   Molecular BioSystems

Polymer-assisted iron oxide magnetic nanoparticle immobilized keratinase

(2009) Rocktotpal Konwarh et al.   NANOTECHNOLOGY

Synthetic biology: The yin and yang of nature

(2009) Jeff Gore et al.   NATURE

PREPARATION AND ACTIVITY OF BUBBLING-IMMOBILIZED CELLOBIASE WITHIN CHITOSAN-ALGINATE COMPOSITE

(2009) Fang Wang et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase

(2009) C. J. Jackson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational design-based molecular engineering of the glycosyl hydrolase family 11 B. subtilis XynA endoxylanase improves its acid stability

(2009) T. Belien et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Protein structure prediction begins well but ends badly

(2009) Rhodri Saunders et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Influence of Silica-Derived Nano-Supporters on Cellobiase After Immobilization

(2008) Peng Wang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhancement of thermostability of fungal deglycating enzymes by directed evolution

(2008) Kozo Hirokawa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

An efficient immobilizing technique of penicillin acylase with combining mesocellular silica foams support and p-benzoquinone cross linker

(2008) Anming Wang et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation

(2008) M. Yakup Arıca et al.   BIORESOURCE TECHNOLOGY

Truncation of the cellulose binding domain improved thermal stability of endo-β-1,4-glucanase from Bacillus subtilis JA18

(2008) Yujuan Wang et al.   BIORESOURCE TECHNOLOGY

Improvements of enzyme activity and enantioselectivity via combined substrate engineering and covalent immobilization

(2008) Pei-Yun Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Spherezymes: A novel structured self-immobilisation enzyme technology

(2008) Dean Brady et al.   BMC BIOTECHNOLOGY

Understanding enzyme immobilisation

(2008) Ulf Hanefeld et al.   CHEMICAL SOCIETY REVIEWS

A Three-Enzyme Cascade Reaction through Positional Assembly of Enzymes in a Polymersome Nanoreactor

(2008) Stijn F. M. van Dongen et al.   CHEMISTRY-A EUROPEAN JOURNAL

Progress and challenges in protein structure prediction

(2008) Yang Zhang   CURRENT OPINION IN STRUCTURAL BIOLOGY

Galacto-oligosaccharides production during lactose hydrolysis by free Aspergillus oryzae β-galactosidase and immobilized on magnetic polysiloxane-polyvinyl alcohol

(2008) David F.M. Neri et al.   FOOD CHEMISTRY

Preparation and characterization of cross-linked enzyme aggregates (CLEA) of Subtilisin for controlled release applications

(2008) K. Sangeetha et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Engineering Hyperthermostability into a GH11 Xylanase Is Mediated by Subtle Changes to Protein Structure

(2008) Claire Dumon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Improving enzyme immobilization in mesocellular siliceous foams by microwave irradiation

(2008) Anming Wang et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Directed evolution of a maltogenic α-amylase from Bacillus sp. TS-25

(2008) A JONES et al.   JOURNAL OF BIOTECHNOLOGY

Novozym 435 displays very different selectivity compared to lipase from Candida antarctica B adsorbed on other hydrophobic supports

(2008) Zaida Cabrera et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Direct Site-Selective Covalent Protein Immobilization Catalyzed by a Phosphopantetheinyl Transferase

(2008) Lu Shin Wong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nanobiocatalysis and its potential applications

(2008) Jungbae Kim et al.   TRENDS IN BIOTECHNOLOGY

Conformational flexibility of a model protein upon immobilization on self-assembled monolayers

(2007) Saharnaz Bigdeli et al.   BIOTECHNOLOGY AND BIOENGINEERING

In Crystallo Capture of a Michaelis Complex and Product-binding Modes of a Bacterial Phosphotriesterase

(2007) Colin J. Jackson et al.   JOURNAL OF MOLECULAR BIOLOGY

Stabilization of a Multimeric β-Galactosidase from Thermus sp. Strain T2 by Immobilization on Novel Heterofunctional Epoxy Supports Plus Aldehyde-Dextran Cross-Linking

(2004) Benevides C. C. Pessela et al.   BIOTECHNOLOGY PROGRESS